Application Note
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Cell Culture
®
Corning Matrigel Matrix
Corning Matrigel® Matrix offers you a more complete basement
membrane preparation for better cell growth and differentiation.
C
orning Matrigel® Matrix is a solubilized basement
membrane preparation isolated from EngelbrethHolm-Swarm (EHS) mouse sarcoma, which is a tumor
rich in extracellular matrix proteins. Its major components
include laminin, collagen IV, enactin, and heparin sulphate
proteoglycan. It also contains growth factors, namely
TGF-β, epidermal growth factor, fibroblast growth factor,
insulin growth factor, tissue plasminogen activator, and
other growth factors that
occur naturally in the EHS
tumor.
This model system closely
mimics the structure,
composition, physical
properties, and functional
characteristics of the
basement membrane in
Matrigel® Basement
Membrane Matrix
vivo, so that cells behave
as they do in vivo when
they are cultured on Corning Matrigel® Matrix. This system
provides a physiologically relevant environment for studies
of cell morphology, biochemical function, migration or
invasion, and gene expression.
Product Information
Matrigel® Applications
Cell Growth and Differentiation
Corning Matrigel® Matrix is especially suited for the culture
of polarized cells, such as epithelial cells. It promotes the
differentiation of many cells types, including hepatocytes(1),
neurons(2), mammary epithelial(3), endothelial(4), and smooth
muscle cells(5) as well as various other glandular cells(6).
In Vivo Angiogenesis Studies
Corning Matrigel® Matrix HC can be used to assess in vivo
angiogenic activity of different compounds by subcutaneous
injection into mice (Corning Matrigel® Plug Assay). The plugs
are subsequently removed and analysed for the formation of
blood vessels. The use of Corning Matrigel® Matrix has been
demonstrated in several in vivo studies(7, 8, 9).
Augmentation of Tumor Growth in Nude Mice
Corning Matrigel® Matrix HC has been shown to promote
successful transplantation of many human tumor cells,
including prostatic, breast, small-cell lung, colon, adrenal
carcinomas, melanomas, and lymphoblastic leukemia cells.
Also, it has been found to increase tumor growth rates in vivo.
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REF NO: MKTAUAPP00002 You can find these articles
in full online at: www.invitro.com.au/applications
Product
Application
Detail
Product Code
Corning Standard
Matrigel® Matrix
General cell culture for example 2D
and 3D cultures, angiogenesis and
cell invasion assays
Is suited for application where a more
highly defines basement membrane
preparation is desired
Is recommended for assays which
require colour detection
Is suited for in vivo applications
where a high protein concentration
augments growth of tumors
Typical protein concentrations
for Corning Matrigel® Matrix are
between 9-12 mg/mL
Available in standard GFR,
High Concentration (HC), and
Phenol Red-Free formats.
Also available in standard and
high concentration
Matrigel® Matrix High
Concentration is 18-22 mg/mL
FAL356234, FAL354234,
FAL356235
Growth Factor
Reduced(GFR)
Phenol Red Free
High Concentration
(HC)
FAL356230, FAL354230,
FAL354263, FAL356231
FAL356237, FAL354262
FAL354248
Corning Matrigel® Matrix Pre-Coated Products
Product Code
Corning BioCoat™ Matrigel® Invasion Chambers 8.0um (6-well)
Corning BioCoat™ Matrigel® Invasion Chambers 8.0um (24-well)
Corning BioCoat™ GFR Matrigel® Invasion Chambers 8.0um (24-well)
FAL354481
FAL354480
FAL354483
Customer Care
T 1300 156 862
F 1300 552 004
E care@invitro.com.au (general enquiries)
orders@invitro.com.au (purchase orders)
www.invitro.com.au
Complement your Migration
and Invasion assays with
Falcon Cell Culture Inserts
Available in several membrane pore sizes to suit your application!
Applications
Cell-based assays on microporous
membranes provide predictive in vitro
models for:
• Cell Migration and Invasion Assays
• Angiogenesis
• 3D Cell Culture Assays e.g. in breast
cancer and cell differentiation assays
• Cell Imaging
• Cell Differentiation and Functionality
during Co-culture
• Drug Bioavailability Studies.
The Falcon Insert Systems offer the
following advantages:
• Improved fluid handling
• Easier media changes
• Improved insert stability in the well.
Also available
Falcon Companion Plates:
Specifically designed for use with
Falcon Cell Culture Inserts to eliminate
evaporation and contamination
caused by an improper lid fit.
Falcon Cell
Culture Inserts
Product Information
Product
Code
Description
Pack
No. per
Case
FAL353102
FAL353097
FAL353502
FAL353504
Falcon Cell Culture insert for 6 well Plate with 1.0 µm Pore Transparent PET membrane
Falcon Cell Culture insert for 24 well Plate with 8.0 µm Pore Transparent PET membrane
Falcon 6 well Clear Flat Bottom TC-Treated Permeable support Companion Plate with lid
Falcon 24 well Clear TC-Treated Cell Culture insert Companion Plate with lid.
1
1
1
1
48
48
50
50
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*For a full list of references, email: info@invitro.com.au
Customer Care
T 1300 156 862
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orders@invitro.com.au (purchase orders)
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CORNING Matrigel® MATRIX References:
1. Bi, et al, 2006. Drug Metab. Dispos. 36:1971
2. Uemera et al 2010. Journal of Neuroscience Research
88(3): 542–551
3. Pires et al, 2013. Cancer Biology & Therapy 14:3,
246–253
4. Takeda et al 2007. Blood. 109:1524
5. Zbinden et al 2010. Arteriosclerosis, Thrombosis, and
Vascular Biology. 2010; 30: 477-482
6. Klarmann et al 2009. Clinical & Experimental Metastasis.
26(5): 433-446
7. Bid et al 2012. Mol Cancer Ther 11:649-659.
8. Coltrini et al 2013. Angionenesis 16(2): 469-477
9. Guedez and Stetler-Stevenson 2012. The Textbook of
Angiogenesis and Lymphangiogenesis: Methods and
Applications, pp 305-309
Customer Care
T 1300 156 862
F 1300 552 004
E care@invitro.com.au (general enquiries)
orders@invitro.com.au (purchase orders)
www.invitro.com.au