S t u d e ntGu i d e Name 22-1448 Date Dogfish Shark Dissection Introduction During this activity you will observeand dissecta shark to developa better understandingof vertebrate anatomy'The dogfishsharkis an excellentmodel organismfor comparativevertebrateanatomydue to its sizeand availability.Studyingdogfishanatomyalsohelpsprovide someinsighrinro verrebrate evoludon and classification.As you proceedthrough this investigation,ysu will be askedto consider how the form of a particularstructurefits its function. Correlatingstructureand function is an underlyingthemein all anatomicalstudies. Background Squalus a.canthias is one of the most common typesof migratorysharks,the spinydogfish.Dogfishtravel in largeschools,often composedo{ individualsof the samesizeand sex.Dogfishmay live up to 30 years. They reachsexualmaturity at about 6-12 years.Dogfishear a variety of small fish and marine animals such assquid,shrimp,crabs,and octopus.They are eatenby largerfish (especiallyother sharks)and by marine mammalssuch assealsand killer whales.While the name "spinydogfish"seemsominous,rhis sharkposeslittle threat to humans.A poisonousspinelocatedon the dorsalfin is usedfor protection againstpredators'The dogfishsl-rarkis often seenasa nuisanceto fishermanas theseanimalsshred through fishingnets to reach tl-reirfood. Another interestingfeatureof tl-iedogfishis its lengthy gestationpericld,up to 2 yearsl O2 00 8 Ca r olina Biologic al Supply Co m p a n y 9-1 DogfishDissection Name S t u d e ntGu i d e Date Pre,Lab ObservationSheet Part 1. Directional Termsand Body Planes Review the following directional terms and body planes. Write a definition for each term. Then, label the directionaltermsand planeson the dogfishillustration.Items markedwith an asrerisk(x) are not in the illustration. Direction Definition Lateral- Medial - Proximalx- Distal* - Dorsal- Ventral- Anterior (cranial)- Posterior(caudal)- S"n^.fi.i"l* - DeepxPlane Definition Sagittalplanex- Midsagittalplane* Tiansverseplane- Frontal plane- @2 00 8 C ar olina Biologic al Supply C o m p a n y 9-2 DogfishDissection Name S t u d e ntGu i d e Date Pre,Lab Observation Sheet (cont.) Orientation of Bodv Planes and Directions plane plane 02 00 8 Car olina Biologic al Supply Co m p a n y 5-3 DogfishDissection Name S t u d e ntGu i d e Date Pre'Lab Observation Sheet (cont.) Paft 2. Characteristicsof the Dogftsh Shark 1. What have you learned about the dogfishshark in terms of physical characteristics,range, habitat, behavior,reproduction,and food habits. 2. Why is the dogfisha good choicefor study and dissectionl 3' What are three questionsyou have concerningthe dog{ishshark that can be answeredonly through dissection? O20 08 Ca r olina Biologic al Supply C o m p a n y 5-4 DogfishDissection Name S t u d e ntGu i d e Date Procedure This dissectionprocedureis divided into severalparts,eachpart addressinga di{Ierentbody system. Beforeyou begin this dissection,read the entire procedurecarefully.As you follow the step-bystep instructions,any observationsand answersshouldbe recordedon the DogflshDisectionObservation Sheetprovidedby your instructor.Use the diagramsto reconcilewhat you have learnedfrom the procedure.And remember-form fits function. Part I. External Anatomy 1. The spinydogfishsharkis an agileswimmerwith a long, streamlinedbodir k possesses a caudal fin, two singledorsal fins, one pair of pelvic fins, and one pair of pectoral fins. Referto the figure below to locateeach type of fin on your specimen.Payparticularattention to the largepoisonsecretingspinethat precedeseach dorsalfin and the asymmetricalshapeof the caudalfin. Basedon the shapeand locatior-rof each {in, what type of funcrion, or movement,do you think it might provide the sharkl Recordyour ideason the ObservationSheet. 1 . Mouth 4 . Pelvic fin 7. Posteriordorsalfin 7. Gill siits 5. Lateral line 8, Anterior dorsalfir-r 3. Pectoralfin 6. Caudal fin 2. The skin is coveredb)'tiny placoid scales,or dermal denticles, with sharpspinesthat project posteriorly.Ycrucan run your hand over the shark'sbody from tail to head to feel this rough texture. Run your hand in the oppositedirection and it feelssmooth.Amazingiy,thesescalesare considered modificationsof teeth and consistof enameland dentin. What benefit might the configurationof rhesescaleshave to a shark'sswimmingabihtyt Recordyour answeron the ObservationSheet. Placoidscale 4x magnification O2 00 8 Ca r olina Biologic al Supply Co m p a n y 9-5 3 . Locatethe narrow horizontalstripealongeachsideof the body of your shark.This interesting characteristiccommon to all fish is calledthe lateral line systenr.. Using your hand lens,observethe smallporesfound along this line. Tl-reyare openingsthat lead to sensitivenerve receptorsbelow the skin. What purposemight this lateral line systemhave to a fish, especiallyin rnurkywatersl Record your thoughtson the ObservationSheet. 4 Examinethe head of your specimen.The pointed snout is called the rostrum. Orr the undersideof the rostrum,the nares,or nostrils,allow water in to moistenthe olfactorysensorycellsand permit the sl-rarkto detect odorsin the environment.The eyesare very similar to human eyes,rvith an iris, pupil, cornea,and conjunctiva.just beirind the eyes,locate two tiny pores.Theseopeningslead deepvvitirinthe braincaseto the inner ear,the organof equilibrium.Horv doesthe taperedrostrum benefit the shark?Recordyour ideason the ObservationSheet. 5, Next locate tl-relargeopeningscalledthe spiracles,which serveaspassageways for water into the mouth. This rnakesit possiblefor rvaterto be bror,rghtin to tire gills for respirationwl-ienthe mouth is closed.How rvouldthis benefit a bottom-dwellir"rg sharkor rayJRecordvour ideason the ObservationSheet. 6. Determine the sexof your specitnen.Use the diagramsbelorvto comparenale and femalesharks. The nralespecimenhas a noticeablefinger-likestructurecalled a clasperon tl'remedial sideof each peivic fin. During copulation,the clasperis insertedinto the femaie{or transferof sperm.Record the sexof vour soecimenon the ObservationSheet. Part II. Internal Anatomy 7. Place the clogfishon the dissectir-rgtray rvith the ventral side facing up. Using a scalpel, make an incision just anterior to the pelr.ic fins. Because the skin is tough, you lna)'need to use scissorsto cut thrc''ugh the bt.{y rvall from the pelr'ic fins anteriorly to rhe riohr nec.roralfin. Make an incision at t1-iebase ot ' t hc l e ft p e c tu ra lfi n rrn dc u t c a l eful l y through the body wallback to the pelvic girdle. Then, make a transverse incision across the pelvic girdle (frorn the rigl'rt pectoral fin to the left pectoral fin). Contir-rue tl-reincision L-'osteriorlyto the peh,ic fins. Tl-ris incision should resemble a triar-igle.Remove the rrentral body rvall. You har.e now expcisedthe coelon, the large central body cavity found in all ieriebrates. In the shark, it is divided into trvo parts: the large posterior portion called the pleuroperitoneal cavity, and the small anterior portion called the pericardial cavity, which contains the heart. How rvoul.l an airbreathingvertebrate (such as a human) be ditferent from the.1ogfish in terms of its body cavitiesJ Refer tcr the following diagram, if needed, irnd recorcl your ideas on the Observation Sheet. O2008 C ar o lir - a Bio lo g ca l Supply Co n r p a n y ?- o Esophagus /4' . ::rt; Heart Smail intestine Liver Liver Stomacl-r Laf {L lntestlne q^t-^- Esophagus Stomach Small intestine :1.: rl:, ,lril,. ;Hi ig,liil 8. The most conspicuous organ in the pleuroperitoneal cavity is the liver. Note the eh 'ngated right and left lobes and the median lobe. In addition to producing bile and detoxifyir-rgwastes, the liver also stores energy in large amounts of oil, wl-rich can provide limited buoyancy in the absence of a swim bladder. How does tl-ie oil pror.'idethis abilityl Record yolrr answer on the Observation Sheet. The DIGESTIVE SYSTEM is responsible for chemically and mechanically breaking dorvn fuod intcr smaller compounds that can be released into the bloodstream and then transported to body cells. Let's investigate the specializedorgans of this system. 9; Using the diagrams provided, locate the najor orgarls of the digestive system, including the esophagus, stomach, small intestine, and colon. You rviil have to move the long liver lobes gently to tl-reside to see the esophagusand stomach. As you locate and observe these organs, provide a detailed description (relative size,shape,color) of each on your Observation Sheet. 10. Examine the esophagusand J-shaped stomach more closell'. Because it is sometimes difficult to distinguishthesetwo organsexternally,observe their internal structure.Make an incision and tl-rroughthe ventral rvali of the esophagus stomach.Open both organs, extend down to t1-re noting the stomachcontents,if an1,,on yout observationsheet.\bu tnay need to washout conrer'Itsto \/iew the lining. Tl're lining of the is coveredu'ith small,finger-iike esophagus projectionscalledpapillae,rvhile tire stomach lining has deepfolds calledrugae. Basedon your obsenations,which organis more suitedfor mechanicaldigestionof food?Explainyour icleas on the Obsen ation Sheet. 02 00 8 Ca r o"na Biologic al Supply Co m p a n y Papillae 5-7 11. The contentsof the stomachemptyinto the U-shapedduodenum,the {irst of threesectionsof the small intestine. At this point, digestivelluids are secretedinto the small intestine from two important accessory organs,the gallbladderand the pancreas.Locatethe gallbladderalongthe right edgeof middle lobe of the liver. It is responsiblefor concentratingand storingbile that is producedby the liver. Bile providesfor the emulsificationof fats.The lightly coloredpancreascan be founclat the curveof pancreaticjuice, a cocktailof digestive the duodenum.This two-lobedglandularorganreleases chemicals,into the duodenum.From the duodenum,foodstufftravels to the noticeably largervah'ular intestine,whoseouter surfaceis markedby rings.Cut open this structureby makinga shallowincision alongone side.Youwill seesomethingunique.In sharks,the spiral valve servesto increasesurface areain a very short intestine.How is this increasein surfaceareaaccomplished in highervertebrates, Recordyour answelon the ObservationSheet. suchasmarnmals? 12. The colon is a narrowedcontinuation of the valvular intestine.It terminatesat the anus, which opensinto the cloaca.Leadinginto the colon, a slender,finger-likestructurecalled the rectal gland excretessalt as a meansof regulatingexcessiveconcentrationsin tire blood. Why ra,ssli a shark needto regulateits saltconcentrations? Recordyour ideason your ObservationSheet. The RESPIRATORY SYSTEM functionsin gasexchange,in which outgoingcarbondioxide u,asteis replacedwith freshoxygento the bloodstream.The CIRCULATORY SYSTEM is responsiblefor the gases, transportbf respir:rtory nutrients,hormones,def'ense celis,and wastesthroughoutthe bodl'.Dissection of tl-ieprimitivesharkprovidesan excellentopportunityto conpare to moreadvancedrrertebrates. 13. In fish, respirationoccursby the diffusionof oxygenand carbon dioxiclebetweenthe water and specialized structutescalledgills. Tb exanine thesestructllres,insert the scissorsinto the right cornerof 1,surshark'smouth. Cut posteriorlythrough the jaws and acrossthe externalgill slitsas far asthe pectoralgirdle.Finally,cut acrc)ss the ventral musclesto open this flap alongyour dissection tray and securervith pins.Ycruharreexposedthe pharynx, the muscularchamberthzrtextendsfrom Along the lateral walls,locate the five pairsof internal gill slits. the t rai cavity to the esophagus. They lead to tl"regill pouches,u'hich ir-rturn ieaclto the external gill slits. The gi1lslits are protected b1's[rsslaltinger-likestructurescalledgill rakers that act as strainersto block food particies.Norv examinethe cut surfacesof the gilis.Thesehighly fol.led filamentousgill lamellae are the primary " respirattrrl'por-titrrof the gilis.Using your haud lens,carefullyobservetheselamellaeto notice tirat eachis coveredby tiny, closelypackedsecondarylamellae.Hor.r'is tl'restructureof thesegills suited to tl-reirfunction in respiratic'rn? Recordyour ans\veron the ObservationSheet. Pharlr-rx External gill slit trlil lamellae Spiracle , raKers O20 08 Ca r olina Biolog c al Supply Co m p a n y 9-B 14. Now examinethe pericardialcavity anclheart. The dogfishI'rearthas four chambersarrangedir-ra tube-likeconfiguration:sinus venosus,atrium, ventricle, and the conus arteriosus.Using the diagramsprovided,locate thesefour chambersin your specimenand trace the path of deoxygenated blood through the l-reartbeginningwith the sinusarteriosus.Unlike the heart of mammalsand birds, the heart of the shark transportsonly deoxygenatedblood. \Vl-reredo you think rhe blood is oxygenatedin the sharkl Recordyour thoughtson rhe ObservationSheet. Conus arteriosus :&',,,,, ff , ..i*!!!S Ventricle The UROGEI{ITAL SYSTEM ir-rch-rdes both reproductive and excretory organs and structures. These systemsare often stlldiecl together because they shiire common ducts. The main purpose of the excretor,v organs (kidneys) is to filter nitrogenous wastes from the blood, producing urine. Ti're reproductive organs. of course, lre responsible fcrr the production of the egg and speln-iand the necessarymeans for the union of these tu,o special cells. 15. Returnir-rgto the pleuroperitoneal cavit)-,remove the liver at its anterior end. If you have not removed it alreadl',relnove the digestive tract from the anterior end of the esophagusto tl're posterior end of the colon. This u'iil reveal the slender paired kidneys in your specimen iocated along the clorsalwall lateral to the midline. While the kidneys are the primary organs of excretion, will -vou lcern that thel'also have a reproductive role in the male shark. Considering the sex of your specinen, rr'hat specific structures do you expect to observe?Record your ideas on the Observation Sheet. If 1*ourspecimenis male, contirTuewith ste116. If ,-our specimenis female, skipto srep'18.You willbe expected to be familiar witl't structtLres of eachsc.x,so it is impnrtant to work witl't another grottp that."hasa sharkof the opposrce sex. 16. Locate the paired testes acljacentto the cranial end of the kidr-reys.Minute ducules carry slrerm prc'duced in the testesto the anterior portion of the kidney called the epididpnis. The sperlr then travel tl'rrough the ductus deferens, ilhicl'r widens into t1-reseminal vesicle. ]n an immature specimen this tube will appear straight in comparison to the coiled tube of a mature male. The sperm-cunraining fluid is received by the paired sperm sacs (located just dorsal to the cloaca) and then passesthrough the ckraca to exit the body. (Ttre cloaca is also the exit for rectal rvasteand urine.) Study the diagrams belou' that c('mpare the shark anatoilly to that of a l-righervertelrrate. Explain the primitive nature of the shark's urogenital s)rstem.Record your ideas on the Obsen ation Sheet. 02 00 8 Car olina Biologic al Supply Co m p a n y 5-9 Spermsac 17. How is sperm transferred frorn the male to female during copuladon? The previously identified claspers (located on the medial aspect of the pelvic fins) each have a dorsal groove that carries the fluid from the cloaca to tl-ie female. Also associatedwith the clasper is a thin-walled siphon that is connected to the dorsal groove just under the skin. You can make a transverse cut into the ventral surface of a pelvic fin to locate this structure. The siphons secrete a ir-ibricating fluid for the claspers. On your Observation Sheet, trace the path of sperm frctm its production in the testes to its release into the female. 18. In the female, loczite the large, paired ovaries adjacent to the anterior end of the kidneys. If vour specinen is immature, these egg-producing organs r.r'illappear quite small and smooth. Use the di:igram below to locate the paired oviducts ancl uterus in your specimen. The eggs are released from the ovary into the oviducts through a smallpore called the ostium. (lt is difficulr to see in mosr specimens.) The egg is fertilized here, then passesthrough tire shell gland, rvhich secreres a rhin metnhrane around the fertilized egg.From there, the egg continues down the oviduct to the uterus, rvhere gestation occurs. As the ),'ounggroq they are attached to the nourishing yolk sac by means of a stalk. Tl-rismethod of development in tl"redogfish shark is known as o+touiuiparous.Chickens are ouiparous, and humans are uiuiparous. Explain the differences on your Observation Sheet. Oviducts Devekrping young Yolk sac Ovar1, O20 08 Ca r olina Biologic al Supply Co m p a n y 5-10 DogfishDissection Name S t u d e ntGu i d e Date Dogfish Dissection Observation Sheet fins I Basedon the shapeand location, what type of function or movement does each fin provide the dogfish sharkl caudal fin: dorsal{ins: pelvic fins: pectoral fins 2 3 placoidscales \What benefit might the configuration of the placoid scaleshave to a shark'sswimming ability? lateralline system What purposemight the lateral line systemhave to a fish, especiallyin murky warers? rostrum 4 How does the tapered rostrum benefit the shark? spiracles ) How would being able to bring water in to the gills when the mouth is closed benefit a bottom.dwelling shark or ray? gender (male or female) 6 02 00 8 \il/hat genderis your specimen? E Mul" Car olina Biologic al Supply Co m p a n y E Female 5-11 body cavity 7 Compare the body cavity of the dogfish shark to a human. How are they different? liveroil 8 How does the oil from the liver provide limited buoyancy in the absenceof a swim bladder? maior organs of the digestive tract W'ritea detaileddescription(relativesize,shape,color) of eachof the followingorgans: 9 esophagus: stomach: small intestine: colon: esophagusand stomachlinings 10 1l t2 02 00 8 What are the contents of the stomach (if anyd-ring)/ Cornpare the lining of the esopl'ragus and the lining of the stomach. Wl-rich organ is rnore suited for mechanical digestion of food? spiral valve Comparethe smaliintestineof the dogfishsharkand the smallintestineof a mammal. How is the insidesurfaceareamaximizedin eachanimal? rectal gland !7hy would a shark need to regulateits internalsaltconcentration? C ar olina Biologic al Supply C o m p a n y 5- 12 I.3 gills How is the structure of the gills suited to their function in respiration? oxygenation I4 15 Unlike the heart of mammals and birds, the heart of the shark transporrs only deoxygenatedblood. lil/here do you think the blood is oxygenaredin the shark? reproductive structures Considering the sex of your specimen,what specificstructuresdo you expect to observe? male urogenitalsystem t6 I7 18 Compare the shark anatomy to that of a higher vertebrate. Explain the primitive nature of the male shark'surogenitai system. path of sperm Dralva diagramor simplesketchof the path of spermfrom productionin the testes to its releaseinto the female. ovoviviparous,oviparous,viviparous Explain the differencesof each method of offspring development listed below: o\/ovlvlparous: ovlparous: vlvlparous: @20 08 C ar olina Biologic al Supply C o m p a n y 5-13 DogfishDissection Name StudentGuide Date Questions 1. Discussthe hydrodynamicsof the dogfish shark. 2. '!7hat are the two classesof fishl In which classis the sharkl 3. Draw the digestivetract of the sharkfrom mouth to anus.Label the structures. @2 00 8 Ca r olina Biologic al Supply Co m p a n y 9-14 4. What is the advantageof the spiral valve in the shark'sintestine?How doesthis differ from the extremelylong intestine of the humanJ 5. How do the gills function in respiration? 6. What advantages does a caftilaginous skeleton have over a bony skeleton? @20 08 Car olina Biologic al Supply C o m p a n y 9-15 DogfishDissection Name St u d e n tGu i d e Date Glossary Ampullae. Senseorgansthat form a network of jelly-filledcanals. Anterior chamber.The fluid-filled spaceinside the eyebetweenthe iris and the cornea. Anus. The openingthrough which solid wasteis eliminatedfrom the body. Atrium. Either of the two upper chambersof the heart. The left atrium receivesoxygenatedblood into the heart via the pulmonaryveins,and tl-reright atrium receivesblood from theluperior and inferior vena cava, Bile. A greenish'yellowfluid secretedfrom the liver that is releasedinro rhe duodenumof the small intestine.Bile aidsin the processof digestion. Caudal fin. The tail fin, or main propeiling fin of a fish. Ciliary body. Thin, vascular,rniddlelayerof the eye,locatedbetu,eenthe scleraand the rerlna. Claspers.Pairedmale copulatoryorganslocatedon the rear baseof the pelvic fi1s. Cloaca. Tl-reposterioropeningthat servesas the openingfor the intestinal,urinar1,,and genital tracts. coelom. A secondaryb.dy cavity thar surroundsthe digestivesystem. Colon. Largeintestine;functionsin formation of fecesfrom undigestedremainsof food through the reabsorptionof water and action of intestinalbacteria. Conjunctiva. The thin, transparenttissuethat coversthe outer surfaceof the eye. Conus arteriosus.Tl-recavity of the heart which begir-rs at the suprayentricularcresta1d e.ds in the pulmt'rrary rrunk. Cornea. Tiansparentcoveringthat allowslight to enter the eye;in a preservedspecimen,the eyeis cloudy. Dermal denticles (placoid scales).Tiny scaleswith sharpspinesrhat cover the skin of tl-reshark. Dorsal fin. Fin locatedon rhe back of a fish. Ductus deferens.Tlrbethat carriesspermfrom the testis (via epididymis)to the urethra. .-.. Duodenum. The {irst part of tl'resrnallintestine,connectingthe stomachto the jejunum. This is the location of further breakdownof food a{terbreakdownin the stomach Epididymis. Connectsthe testisto the vas deferens.Also servesas a srorageareafor spermoroducedbv the testisbut not yet ejaculated. Esophagus.Musculartube through which food passesfrom the pharl,nx to the stomach. Gallbladden Small sacattachedto the liver; storesbile producedby the liver. Gill arches.The bony or cartilaginoussupportto which gill filamentsand gill rakersare atrached. Gill lamellae.An arrangementof thin platesthat increasesurfaceareafor gasexchange. Gill pouches.Area berweenthe internal and exrernalgill slits. Gill rakers. Bony or cartilaginousprojectionsthat poinr forward and inwarclfrom tl'regill arches. Gill slits. Gills with individual openingsrather than a' outer cover. Gill. A highly vascularrespiratoryorgan through which oxygenis obtainedfrom warer. Iris. Diaphragmthat regulatesthe sizeof the pupil. Kidneys. Rean'shapedexcretoryorgansrespor-rsible for filtering excesswater and wasteprodlictsfrom the blood, Lateral line. Senseorgan usedto detect nlovenent and vibration in the surroundinswater. O20 08 Ca r olina Biologic al Supply Com p a n y 5-16 Lens. Biconvex,transparentstructurethat focusesthe light coming in througl-rthe corneaand pupil of the eye. Liven Accessorydigestiveorganwith many functions,including fat digestionand storage,bile production,glucosemetabolism,and detoxification;the largestvisceralorgan. Nares. Nosrils. Incurrent openingsfor the respiratorysystem. Ostium. Small poresin which eggsare fertilizedand passfrom the ovariesto the oviduct. Ovaries. Femalegonads.Releaseeggsinto the fallopiantubes. for eggcellsfrom the ovariesto the uterus. Oviducts. Fallopiantubes;passageway Pancreas.Accessoryglandwith both an endocrineportion, producinginsulin and glucagon,and an exocrineportion, producingdigestiveenzymes. Papillae.Srnall,finger-likeprojectionscoveringthe esophagus. Pectoral fins. Pairedfins iocatedon each side,usuallyjust behind the operculum. Pectoral girdle. Portion of the appendicularskeletonsupportingthe forelimbs. Pelvic fins. The most posteriorpairedfins of a fish, usedfor stability. Pelvic girdle. Portionof the appendicularskeletonsupportingthe hind limbs. peritoneumthat enclosesthe heart; surroundedby pericardialfluid that Pericardium. Sacof specialized cushionsand protectsthe heart. Peritoneum. Thin membranethat lines the abdominal cavity. Pharynx. The muscularchamberthat extendsfrom the oral cavity to the esophagus. Placoid scales(dermal denticles). Tiny scaieswith sharpspinesthat cover the skin of the shark. Pleuroperitonealcavity. Largerposteriorportion of the coelom. Pupil. The openingthrough which light entersthe eye. Rectal gland. Salt-secretingorgan that regulatessalt concentrationsin the shark'sblood. Retina. Light-sensitiveportion of the eye,composedof receptorcellscalledconesand rods. Rostrum. Structureof the anterior,ventral regionof the cerebrum,extendingventrallyfrom dre genu rd the septumpellucidum. foldsin the wall of the stomachthat allow for expansion. Rugae.Severallongitudir-ral Sclera.Outer coveringof the eyeball;a tough, opaquesheetof connectivetissuethat protectsthe inner structuresof tl-reeyeballand helpsmaintain rigidity. Seminal vesicle.Gland locateddorsalto the urinary bladderin males.This gland releasesfluid tl-iat combineswith spermto make semen. Shell gland. Glarld wllich secretesa thin membranearound the fertilized egg. Sinus venosus.Largechanber ofthe heart that receivesdeoxygenatedblood from the body and has a valve that opensto the atrium. Siphon. Thin-walled sacthat secreteslubricatingfluid for the claspers. Sperm sacs.Sperm-collectingsacslocatedjust abovethe cloaca. Spiracle.Small openingfound behind each eye;usedto pump water through the gilis while the animal is at rest. Spiral valve. The lower portion of tl-reintestine. organto the circulatorysystemthat produceslymphocytesand destroysold and Spleen.An accessory defectivered blood cells. Stomach. Organ responsiblefor tl-rebreakdownof food into absorbableparticles.Locatedin the digestivetract betweenthe esophagusarid the small intestine. Testes.Organsresponsiblefor production of spermin males. O2 00 8 C ar olina Biologic al Supply C o m p a n y 5-17 Urinary papilla. The external projection of the male urinary bladder. Urogenital papilla. A small conical tube located just aheadof the anal fin. Urogenital sinus, Cavity that surroundsboth the urethral and vaginal openings. IJterus. Femalereproductiveorganthat holds developingfetuses. Valvular intestine. The second,and much larger portion of the small intestine. Ventricle. One of two lower chambersof the heart; the left ventricle forcesblood into rhe aorra, and the right ventricle forcesblood into the pulmonary arteries. Vitreous chamber. A cavity of the eye posterior to the crystalline lens and anterior to the retina. O20 08 Car oiina Biologic al Supply C o m p a n y 9-18