Keys to Lab –
Sketches should be done in PENCIL, and to the best of your ability. – You can use your lab-book on all practicals. You must find tissue that we are
studying under microscope. (Try not to sketch straight from pictures – you may have to find tissues under the microscope) Labels should be included –
always label blood vessels. Take your time. You should sketch with a histology atlas as a reference.
Title: Skeletal Tissue Histology Lab
Objective: To understand the anatomy of skeletal tissue
Materials: Histology Slides, Microscope, Lab-book, PENCIL
Procedure: Do not copy in lab-book
1. Sketch and label the following types of epithelial tissue labeling (at least) the
following structures in italics.
a. Compact Bone (100X, 400X) At lower magnification – label Haversian
Canal, Haversian System, Interstitial Cells – At high magnification label
Haversian Canal, osteocyte, canaliculi, matrix
b. Endochondral Bone Formation – Monkey Phalanges
i. View macroanatomy using dissecting microscopes – view and label
joint space (JS), joint ligaments (JL), epiphysis which here is
composed completely of hyaline cartilage (E), epiphyseal plate
(EpPl), diaphysis (D), perichondrium (Pch), and periosteum (PO).
The numbered boxes are hot areas that lead to views of increased
magnification for that area.
ii. View ossification area under 100X -Progression from mature
cartilage (Mt) to areas where matrix calcification (CC) has occurred,
to areas where bone has begun to be deposited onto calcified
cartilage (Oss), Sharkey’s Fibers (Sf)
c. Intramembranous Ossification – (100X-400X) Label: P - periosteum; BS bone spicule; O - osteoblasts; Oc - osteocyte
Results
1. 6 histological sketches
Conclusion
What did you learn during this lab?