A CAN OF BULL? Do energy drinks really provide a source of energy? “A Can of Bull?” “Some Popular Energy Drinks” Student Question #1 Which statement best describes your use of energy drinks? A. I have NEVER tried an energy drink. B. I drink an energy drink OCCASSIONALLY. C. I drink an energy drink whenever I need a “BOOST” of energy. D. I drink an energy drink almost DAILY. Student Question #2 I drink energy drinks because: A. B. C. D. They do give me an energy boost. They taste good. They give me an energy boost and they taste good. I don’t drink energy drinks. Student Question #3 The average cost of a canned energy drink is: A. B. C. D. $1 $2 $3 $4 THE CASE – in summary Rhonda, a writer for “Runner’s World” magazine, was asked by her boss, Charley, to research the use and effectiveness of some popular energy drinks. As an team of students, you will help Rhonda by investigating the ingredients in these energy drinks. You will report your findings to the class. DO THE MARKETING CLAIMS MATCH THE SCIENTIFIC FACTS? Energy? Before you begin… You must first understand what is meant when we say something gives us “energy”. What is “BIOLOGICAL ENERGY”? Student Question #4 Food energy is the amount of stored chemical energy in food that is available after digestion and metabolism. The most common value for expressing the amount of energy in food is: A. B. C. D. Calvins Joules Ounces Calories How is food used for energy? We eat high energy organic molecules such as: carbohydrates, lipids (fats), proteins, & nucleic acids. These molecules are broken down through digestion/ metabolic processes. Energy obtained from food must be stored in form of ATP. What is ATP? Adenosine triphosphate (ATP) is the primary energy molecule. ATP provides the chemical energy that powers most cell work. Modified nucleotide: Adenine + ribose + Pi AMP AMP + Pi ADP ADP + Pi ATP How does ATP store energy? Lots of stored energy in each P bond. Bonding of each Pi group is unstable. Instability of P bonds makes ATP excellent energy donor. ATP ADP releases energy ∆G = -7.3 kcal/mole How does people store energy? ATP is too reactive (transfers Pi too easily) respiration 7.3 kcal/mole Short-term energy storage only Carbohydrates & A working muscle recycles over fats are long-term 10 million ATPs per second energy storage Metabolism Glucose is a key to metabolism. • Cells use glucose to build fats, carbohydrates, and other compounds; Cells recover glucose by breaking down these molecules. Metabolism: cells break down (or build up) glucose and produce ATP. Glucose Glucose is a carbohydrate molecule. It is a monosaccharide that is oxidized through cellular respiration or fermentation to produce ATP. In aerobic (O2 present) conditions: Respiration In anaerobic (no O2) conditions: Fermentation Cell Respiration: Overall equation: C6H12O6 + 6O2 6 CO2 + 6H2O + e- + 36-38 ATP’s 3 Stages: •Glycolysis •Kreb’s Cycle (aka CAC) •Electron Transport Chain Aerobic vs. Anaerobic: Comparison of glucose breakdown Fat, carbohydrate, and protein can be used to produce ATP aerobically. Anerobic: GLYCOLYSIS First step in glucose oxidation. Series of 10 chemical reactions (in cytoplasm). Glucose 2 pyruvates NAD+ (an e- carrier) is reduced to NADH End of glycolysis: 2 ATP, 2 NADH, 2 pyruvates Don’t try to memorize this slide! Take aways: •Glucose is broken down through a series of enzymatic reactions yielding: 2 pyruvates, 2 ATPs, & 2 NADH. •Pyruvate feeds into the Kreb’s Cycle. •NAD+ is a co-enzyme. Aerobic: KREB’S CYCLE Occurs in the mitochondria. In the presence of O2, the pyruvate from glycolysis is oxidized to 3 CO2. 6 NAD+ molecules are reduced to 6 NADH 2 FAD+ (another e- carrier) are reduced to 2 FADH2. 2 more ATP’s formed. Don’t try to memorize this slide! Take aways: •Pyruvate enters Kreb’s Cycle. •Yields: 6NADH, 2 FADH2, 4CO2, and 2 ATP. •NAD, FAD, Co-Enzyme A involved. Aerobic: ELECTRON TRANSPORT CHAIN (ETC) Most ATP is produced in the ETC. High energy released by oxidizing NADH & FADH2. Electrons are passed down ETC while protons are pumped across the inner mitochondria membrane. This proton gradient has high potential energy. Don’t try to memorize this slide! Take aways: •Proton gradient drives production of ATP. •34 more ATP made. •Co-Enzyme Q involved. Some Co-Enzymes in Energy Metabolism Your Task, Part 1: Now that you know what ATP is & how it’s produced: Work as a group to determine the following: 1. 2. 3. 4. What is the nature (sugar, a.a., vitamin, stimulant, etc) of each ingredient in your energy drink? What is the physiological role of each in the body? Which ingredients provide energy? Which ingredients contribute to body repair? Part 1, continued: As a group, fill in table on page 7. Also, answer the questions on pages 4 & 8. Part 2 – Qualitative Chemical Analysis: As a group, you will test the energy drinks for: Amino Acids Proteins Carbohydrates Lipids Sodium Chloride You will also test some comparative drinks (e.g. chocolate milk, soda, vitamin water, etc.) Part 2 –Chemical Analysis, continued: Follow procedure as outlined in your handout. READ all instructions carefully. Fill-in tables & answer questions as you work. Wear gloves & goggles for these tests – some chemicals are toxic. Avoid skin contact. Properly dispose of all chemical waste. Fun Facts About Caffeine! Fun Facts About Caffeine! Used by Native Americans via teas and chocolate First isolated in 1819 90% of adults will have a caffeinated beverage daily Americans = highest at 280 mg/day Caffeine: Delicious or Deadly??? Toxic at high dosages http://www.energyfiend.com/death-by-caffeine New Dangers: Four Lokos Alcohol & caffeine Mixing a stimulant and a depressant Nicknamed “Blackout in a Can” Following massive hospitalization of college students = Banned from states Forced to change recipe CLASS DISCUSSION: Let’s talk about each group’s results. You will be evaluated based on the thoroughness of your groups’ research & your individual participation. How do the energy drinks compare? Is caffeine a source of energy? What’s the difference between an energy source and a stimulant? Final Student Question #5 Should you simply buy a soft drink rather than one of these energy drinks when you need an energy boost? A. Yes B. No C. Still undecided