Name Date. 58 External Anatomy of the Frog BACKGROUND The frog is a member of the phylum Chordata because a gristly rod, the notochord, forms along the dorsal midline of the frog embryo. .Frogs are also classified in the subphylum Vertebrata because the adult frog has a segmented backbone, the vertebral column. Humans are also chordates and vertebrates. Although humans belong to the class Mammalia and frogs belong to the class Amphibia, their bodies show many similarities of structure and organization. OBJECTIVt:S In this activity you will observe the external anatomy of the frog. MATERIALS preserved frog (Rana sp) dissecting tray scissors dissecting needles forceps hand lens PROCEDURES AND OBSERVATIONS Part I. External Anatomy a. Examine the b9dy of the frog. It is mostly composed of a head and a trunk. Note that the neck is so short that it is almost nonexistent; But the frog can still move its head a limited amount. 1; What kind of symmetry frog body? is exhibited moist and slippery with mucus secreted mucus glands in the skin. by b. Observe the skin of the frog. Note the darkcolored dorsal side and the light-colored ventral sid~~With a hand lens, look closely at the skin . 1 ~ 2. Is the skin scaly or smooth? 3. How many nostrils are there? by the The frog skin has concealing coloration known as countershading. It tends to make the animal blend into its surroundings. Seen from above in dark water, the frog is hidden because its back is dark. Seen from below, the light-colored ventral skin blends with light coming from the sky. The skin. of the living frog is kept. constantly( surface. c. Observe the large mouth extending posteriorly on both sides of the head. Find the nostrils near the front tip of the head~ The eyes of the frog protrude when they are open. The frog can close its eyes only by withdrawing them into their sockets. The upper eyelid is not movable, and lower eyelid moves only slightly. The frog also has a third eyelid, a transparent nictitating membrane that closes upward over the eye. It protects the eye when the animal is on land, and allows the frog to see underwater. In the human eye, the "third eyelid" is a small fold of tissue in the corner of the eye next to the nose. d. Examinethe eyes of the frog. Try to identify the three eyelids. The outer part of the frog's ear is the tympanum, a flat, oval membrane. Sound waves cause the tympanum to vibrate. The vibrations are transferred to a small bone inside the ear, which in turn stimulates impulses in certain nerve endings. 253 e. Locate the tympanum, which is posterior to the eye on each side of the head. f. Note the two dorsal folds of skin running from the eye to the anus, high between the hindlegs. g. Observe the two forelegs and the two hindlegs. 4. Where are the four legs attached? A living frog sits with its hindlegs folded and ready to jump. i. Examine a hindleg. Attached to the body is the large thigh. The lower part of the limb is called the leg. Note the long ankle. The foot is attached . to the ankle. II .'c\' 6. Describe the structure of the f'd6t' of a hindleg. ." The forelegs are used mainly to brace the body as the frog lands after a jump. They are also used for climbing, but not for swimming. h. Examinea foreleg. 5. Describe a foot and toes of a foreleg. / 7. Draw an external view of the. frog, dorsal side up, with the anterior end at the top. Label the head, trunk, mouth, nostril, eye, tympanum, skin fold, anus, foreleg and foot, and hindleg and foot. Name, Date. Part II. Mouthparts of the Frog The mouth cavity of the frog is lined with a mucus membrane composed of ciliated cells. The large mouth. surface is a part of the frog's respiratory surface. Oxygen and carbon dioxide are exchanged across the mouth membrane. a. Place the frog ventral side up, anterior end toward you. With the scissors, cut the corners of the mouth on each side where the two jaws meet, so that you can open the mouth wi~e. Pull the lower jaw away from the upper jaw until you can see the back of the mouth. The back of the mouth becomes the pharynx (throat). The frog can flip out its tongue to catch insects in flight. When extended, 'the tongue may be 6 to Bcm long. A sticky mucus on the tongue helps to trap the insect. The insect is popped into the pharYnx where it is swallowed whole. Frogs attempt to capture 'any moving objects small enough to be swallowed. b. Examinethe tongue. 1. Where is the tongue attached? . ' 2. Describe the shape of the free end of the tongue. point of your probe into one nostril from the outside. Watch the tip enter the mouth. Then find the glottis, a slit in the raised area at the back of the mouth. Feel the opening with the point of the probe. e. Between the openings of the nostrils in the roof of ~e mouth are paired vomerine teeth, bony ridges attached to the skull. Feel the vomerine teeth with the probe. Then examine them with the hand lens. These teeth are used for holding, not for chewing. 4. Describe the vomerine teeth. , f. On each side of the upper part of the mouth, near the place where the two jaws meet, find the openings to the Eustachian tubes. The tubes connect the mouth with the middle ear. g. At the back of the mouth, posterior to the glottis, is the opening to the esophagus, which Jeads to the digestive system. Push the handle of the scalpel into the pharyn" to locate the opening. The wrinkled membranes indicate how much the pharynx can stretch to accomodate large pieces of food. ' 5. Make a drawing of the inside of the frog mouth. Label the upper jaw, lower jaw, pharynx, tongue, maxillary teeth, opening of nostril, glottis, vomerine teeth, opening to Eustachian tube, and opening to esophagus. c. Run your finger around the inside of the upper jaw., Use your hand lens to see the maxillary teeth. These hold a large insect in place until it can be swallowed. Then examine the lower jaw. 3. Are there any teeth on the lower jaw? The frog breathes through nostrils. To inhafe, the frog lowers the floor of its mouth. The mouth fills with air through the nostrils. The nostrils then close by means of valves. To fill the lungs, the frog raises the floor of its mouth, pushing the air,. )hrough the glottis. The air then passes th1i)ugh the larynx, or voice box, into the lungs. d. The openings of the nostrils are near the ant~rior end of th~ roof of the mouth. Gently push the 255 CONCLUSIONS AND APPLICATIONS 1. Describe countershading in animal coloration. Of what advantage is it for an animal? 10. . ~\I. d1<. ..1." 2. Describe the body covering of the frog. 3. How does a frog close its eyes? Describe the eyelids of a frog. 4. Describe the structure and functions of the frog's forelegs and hindlegs. 5. Explain how a frog captures and swallows an insect.