Downloaded from http://rstb.royalsocietypublishing.org/ on March 4, 2016 Single molecule cellular biophysics: combining physics, biochemistry and cell biology to study the individual molecules of life Papers of a Theme Issue compiled and edited by Mark C. Leake Contents Introduction The physics of life: one molecule at a time M. C. Leake 20120248 Articles Development of new photon-counting detectors for single-molecule fluorescence microscopy X. Michalet, R. A. Colyer, G. Scalia, A. Ingargiola, R. Lin, J. E. Millaud, S. Weiss, O. H. W. Siegmund, A. S. Tremsin, J. V. Vallerga, A. Cheng, M. Levi, D. Aharoni, K. Arisaka, F. Villa, F. Guerrieri, F. Panzeri, I. Rech, A. Gulinatti, F. Zappa, M. Ghioni and S. Cova Reversible optical control of cyanine fluorescence in fixed and living cells: optical lock-in detection immunofluorescence imaging microscopy Y. Yan, C. Petchprayoon, S. Mao and G. Marriott 20120035 20120031 Single-molecule imaging in live bacteria cells K. Ritchie, Y. Lill, C. Sood, H. Lee and S. Zhang 20120355 A critical survey of methods to detect plasma membrane rafts E. Klotzsch and G. J. Schütz 20120033 Imaging the cell surface and its organization down to the level of single molecules D. Klenerman, A. Shevchuk, P. Novak, Y. E. Korchev and S. J. Davis 20120027 Surface-enhanced Raman spectroscopy at single-molecule scale and its implications in biology Y. Wang and J. Irudayaraj 20120026 Towards physiological complexity with in vitro single-molecule biophysics D. Duzdevich and E. C. Greene 20120271 Twisting and subunit rotation in single FOF1-ATP synthase H. Sielaff and M. Börsch 20120024 High-resolution single-molecule characterization of the enzymatic states in Escherichia coli F1-ATPase T. Bilyard, M. Nakanishi-Matsui, B. C. Steel, T. Pilizota, A. L. Nord, H. Hosokawa, M. Futai and R. M. Berry Inferring diffusion in single live cells at the single-molecule level A. Robson, K. Burrage and M. C. Leake High-throughput gene expression analysis at the level of single proteins using a microfluidic turbidostat and automated cell tracking G. Ullman, M. Wallden, E. G. Marklund, A. Mahmutovic, I. Razinkov and J. Elf 1 20120023 20120029 20120025