SEMINARIO
6 de Marzo de 2015, 12:30 PM
Instituto Cajal. CSIC
Avda. Dr. Arce, 37. Madrid
Ramón Reig
Department of Neuroscience
Karolinska Institutet
Stockholm (Sweden)
“Sensory integration in the mouse striatum”
Summary:
The basal ganglia are involved in several motor, cognitive, and emotional functions. The striatum is the input layer
of basal ganglia, receiving excitatory inputs from thalamus, and most cortical areas, including sensory, motor and
association areas. The striatum has been mainly studied with regard to motor and reward related functions but its
functional role in sensory processing is largely unknown. Our objective is to elucidate the role of the striatum in
the sensory processing. To that end we obtained in vivo whole-cell patch-clamp recordings from neurons in the
dorsal striatum and in cortical layer V of the barrel field (BF) in anesthetized mice. We recorded the evoked
responses to ipsi- and contralateral whisker deflections, and responses to simultaneous whisker and visual
stimulus. Recorded neurons were stained and identified by their morphological and electrophysiological
properties, as well as by their biochemical expression of D1 and putative D2 dopamine receptors (direct and
indirect MSNs respectively). Our results show that; A. all neurons recorded in dorsal striatum responded to both
whisker stimulation. B. individual striatal neurons in the dorsomedial striatum integrate multimodal information,
responding to both tactile and visual stimuli. C. the multimodal and bilateral integration was always sublinear,
with maximal summation occurring after synchronization of the onset of tactile and visual responses. D. direct and
indirect MSNs integrate bilateral whisker input differently. E. striatal interneurons (cholinergic and fast spiking)
integrate bilateral tactile information, and at least fast spiking interneurons can also integrate visual, F. that the
responses were mediated by excitatory and inhibitory inputs, with inhibition following the excitation by few
milliseconds of delay. In summary, our results strongly suggest that the striatum acts as a multisensory integration
structure.
Recent publications.- Ramon Reig, Gilad Silberberg (2014). Multisensory Integration in the Mouse Striatum. Neuron 83(5):1200-12.
-Ramon Reig, Yann Zerlaut; Ramiro Vergara; Alain Destexhe; Maria Sanchez-Vives. (2015) Gain modulation of
synaptic inputs by network state in auditory cortex in vivo. The Journal of Neuroscience 35(6):2689-702.
-Lotta Borgius, Hiroshi Nishimaru, Vanessa Caldeira, Yuka Kunugise, Peter Löw, Ramon Reig, Shigeyoshi
Itohara, Takuji Iwasato, Ole Kiehn (2014). Spinal glutamatergic neurons defined by epha4 signaling are essential
components of normal locomotor circuits. The Journal of Neuroscience. 34(11) 3841-3853.
- Sanchez-Vives MV, Mattia M, Compte A, Perez-Zabalza M, Winograd M, Descalzo Vf, Reig R (2010)
Inhibitory modulation of cortical up states. J Neurophysiol. 104(3):1314-24.
-Reig R, Mattia M, Compte, Belmonte C And Sanchez-Vives MV. Temperature modulation of slow and fast
cortical rhythms. J Neurophysiol. 103(3):1253-61
-Reig R, Sanchez-Vives MV (2007). Synaptic transmission and plasticity in an active cortical network. PloS
ONE. 2(8): e670.
- Reig R, Gallego R, Nowak L, Sanchez-Vives MV. Impact of cortical network activity on short-term synaptic
depression. Cereb Cortex.16(5), 688- 695.