St. Luke's Health System Quick Guide Stability Chart CHEMISTRY/IA/URINALYSIS NOTES: • R.T. sensitive INDICATES THAT THE SPECIMEN IS STABLE AT ROOM TEMPERATURE FOR A TOTAL OF 8 HOURS OR LESS These specimens need to be processed and put into 2-8° C as soon as possible and transported in 2-8°C • • • • • All tubes should be spun and serum/plasma separated from cellular material within 4 hours after collection unless otherwise indicated. Clotting of Serum Specimens: Let the clot tube sit vertically at R.T. between 30-60 minutes, then spin using the designated centrifuge and time. Plasma specimens can be spun immediately upon receipt into the laboratory. Ideally specimens need to be stored and transported vertically. The minimum and required volumes are based as if the tests are ordered individually and they are the bare bones amount. Total stability is equal to R.T. time plus the frozen allowance time. 7/16/2013 D. McMahon; revised 6/12/2014 DMC Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred ABG Acceptable Plastic ABG syringeHeparin NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested 0.3 mL 1 mL Stability Heparin Serum 0.5 mL 1 mL Special Transport Conditions or other Information Sample Preparation and/or Centrifugation/ Instrument 18-28°C = R.T. 2-8 °C = Refrigerated 30 minutes @ R.T (ABG) Acetaminophen Physical Characteristics that Compromise Results Do not expose to air, tube cannot be uncapped. Do not use the pneumatic tube system. Whole blood Tube needs to be filled to capacity. Do not remove cap or exposed to air (ANO2) Cannot be delivered on Ice. Blood Gas Instruments/ iStat, ABL90 14 days @ R.T. Vitros (gel is acceptable (ACETA) 14 days @ 2-8 °C 45 days if kept colder than -18°C EDTA plasma Vitros Acetone (See Betahydroxybutyrate) Order Beta-hydroxybutyrate for serum ketones (BHB) Albumin (ALB) Alcohol Serum Heparin plasma Heparin plasma Serum 0.1 mL 0.3 mL 7 days @ R.T Hemolysis 0.1 mL 0.3 mL Indefinite if kept colder than - 18°C 2 days @ R.T. Need to keep tube capped 14days @ 2-8 °C (ALC) Serum Alkaline Phosphatase Heparin plasma Vitros 30 days 2-8°C 0.2 mL 0.4 mL 18°C one month 4 days @ R.T. Vitros Do not draw from a site that has been cleansed with Alcohol or other volatile disinfectants. Hemolysis Separate serum from cells within 4 hours 4 days @ 2-8°C Vitros (ALP) Alpha-1 Antitrypsin Serum 0.1 mL 0.3 mL 4 days @ - 18°C 24 hours @ R.T. Cannot test on sample that has been frozen Vitros - Ref Lab only 7 days @ 2-8 °C Vitros (AAT) ALT (SGPT) Heparin 0.2 mL 0.4 mL 3 days @ R.T. Hemolysis, 0.5 mL 1 mL 7 days @ 2-8°C 3 hours @ 2-8 °C Frozen sample If left @ R.T., the sample needs to be rejected and recollected. plasma (ALT) Ammonia Serum Heparinized Plasma only Whole blood Whole blood R.T. unacceptable 24 hours if kept colder than - 18°C Place tube on ice immediately after drawing Centrifuge immediately and remove from cellular material within 15 minutes of collection. R.T. unacceptable Vitros (AMMON) Serum Heparin 0.1 mL 0.3 mL 7 days @ R.T. Centrifuge specimens and remove the serum or plasma from cellular material within 4 hours of collection. Amylase 30 days @ 2-8 °C (AMY) Anti thyroglobulin AB (ATG) R.T. Sensitive Serum Plasma Heparin plasma Frozen sample unacceptable 0.2 mL 0.5 mL 8 hours @ R.T. 7 days @ 2-8°C put back into refrigerator ASAP Immulite - Ref Lab only 6 months @ - 20°C (ATG) APT Test See Fetal Hemoglobin 1 St. Luke's Health System Quick Guide Stability Chart Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred AST(SGOT) Serum Acceptable Heparin plasma NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested 0.2 mL 0.4 mL (AST) Stability 3 days @ R.T. Serum Heparin Plasma 0.3 mL Heparin Plasma 0.15 mL Serum 0.2 mL ( plasma or serum) 0.4 whole blood on Neonates with high hemacrit 1 mL Serum (Go live Aug. 14, 2013) (BHB) Neonatal Bilirubin (BuBc) Heparin plasma R.T. & Light Sensitive 0.50 mL Heparin plasma 0.1 mL 0.3 mL 6 months if kept colder than - 18°C 4 hours @ R.T. R.T. & Light Sensitive 7 days @ 2-8 °C (BILT, BILD) BNP R. T. Sensitive 0.2 mL 1 mL 6 months if kept colder than - 18°C 4 hours @ R.T. Heparin or EDTA plasma 0.4 mL 1 mL 3 days @ R.T Heparin or EDTA plasma 0.2 mL Heparin Plasma 0.2 mL Whole Blood EDTA (BNP) NT-pro BNP Serum (PBNP) BUN Serum (Urea Nitrogen) 0.4 mL Serum 0.4 mL (C3) C4, Complement Serum Heparin or EDTA plasma 0.2 mL 0.4 mL Serum R.T. Sensitive Heparin or EDTA Plasma 0.3 mL 0.5 mL Serum R.T. Sensitive (CA153) CA 19-9 R.T. Sensitive (LCA199) Serum Immulite - Ref Lab only Vitros Heparin or EDTA Plasma Heparin or EDTA Plasma Protect from light 0.3 mL 0.5 mL Vitros Tube has to be at least ½ full or recollection will be required. Turbidity or hemolysis i-Stat 3 days @ 2-8°C Separate serum from cells with 2 hours 1 years @ -20° Vitros - Ref Lab only 24 hours @ R.T. Separate serum from cells with 4 hours 6 months @ Colder than -18°C 1 day @ R.T. Can Not use EDTA plasma (C4 can) 3 days @ 2-8 °C 4 weeks if kept colder than - 20°C 1 day @ R.T. 4 weeks if kept colder than - 20°C 8 hours @ R.T. 4 weeks if kept colder than - 20°C 8 hours @ R.T. 0.5 mL 4 weeks if kept colder than - 20°C 8 hours @ R.T. 7 days @ 2-8 °C Fasting specimen is preferred Vitros - Ref Lab only but not essential Fasting specimen is preferred Vitros - Ref Lab only but not essential Turbidity Avoid Evaporation Vitros - Ref Lab only Return to 2-8°C ASAP Turbidity 7 days @ 2-8 °C 0.3 mL Vitros If exposed to Keep protected from light laboratory light greater than 2 hours, results may be decreased. 7 days @ 2-8 °C (CA125) CA 15-3 Exposure to light will cause decrease 3 days @ 2-8 °C (C4) CA 125 Lipemia 5 days @ 2-8°C (BUN) C3, Complement put back into refrigerator ASAP 7 days @ 2-8°C 0.3 mL (plasma or 4 hours @ R.T. serum) 0.7 whole blood on 7 days @ 2-8°C Neonates with high hemacrit (BILN) Total &/or Direct Bilirubin Serum 7 days @ 2-8°C 8 hours @ R.T. Re-suspension of platelets can cause false elevation of AST results. Vitros Needs to be centrifuged properly 14 days @ colder than 18°C 8 hrs @ R.T. (B2M) Sample Preparation and/or Centrifugation/ Instrument Avoid agitation or mixing of plasma samples after centrifugation. ↑ hemolysis 1 week @ 2-8°C (serum Interference by only) platelets R.T. Sensitive Beta hydroxybutyrate Special Transport Conditions or other Information 18-28°C = R.T. 2-8 °C = Refrigerated 3 months if kept colder than - 18°C Beta-2-Microglobulin Physical Characteristics that Compromise Results Avoid Evaporation Vitros - Ref Lab only Return to 2-8 °C ASAP Turbidity Avoid evaporation Vitros - Ref Lab only Return to 2-8°C ASAP 4 weeks if colder than 20°C 2 St. Luke's Health System Quick Guide Stability Chart Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred Calcium Serum R.T. Sensitive Acceptable heparinized plasma NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested 0.1 mL 0.3 mL Serum Heparinized (Tegretol) Gel tube not acceptable Plasma 0.3. mL 0.5 mL (LTEG) Carbon Monoxide (CO, Carboxyhemoglobin) (CO) Whole blood EDTA or Heparin 0.2 mL 0.5 mL CEA Serum 0.1 mL 0.3 mL Serum R.T. Sensitive 4 hour @ R.T. Heparin plasma 0.3 mL 0.6 mL (CHOL) Serum Heparin plasma 0.2 mL 0.4 mL (CK) Serum R.T. Sensitive Heparin or EDTA Plasma 0.2 mL 0.4 mL Blood Gas instruments with cooximetry (ABL90'S) 8 hours@ R.T. Avoid evaporation 4 weeks if kept colder than -20°C. 7 days @ 2-8°C Return to 2-8°C ASAP 3 hours @ R.T. 3 weeks if kept colder than - 18°C 4 hours @ R.T. Cord Blood Heparin Serum Heparin or EDTA Plasma (Arterial or Venous) 1 month if kept colder than - 18°C 3 hours @ R.T. 0.5 mL in a 3 mL syringe or 0.15 mL in a 1 mL syringe 0.65 mL 4 weeks if kept colder than - 20°C 60 minutes @ R.T. 0.1 mL 0.3 mL 48 hours @ R.T. Vitros- Ref Lab only extremely As soon as possible store in icteric samples 2-8°C decrease cholesterol result Vitros Hemolysis raises CK As soon as possible store in results. 2-8°C CK results are decreased with icteric samples. Separate serum from cells within 2 hours after collection. Turbidity Separate serum from cells within 2 hours after collection. 5 days @ 2-8°C (CKMB) Cord Blood Gases Vitros Vitros ≤6 months if kept colder than -18°C 4 weeks @ R.T. 5 days@ 2-8°C R.T. Sensitive CK MB As soon as possible store in 2-8°C 1 year if kept colder than - 18°C ≤5 days @ R.T. Gel Tubes are not acceptable ≤5 days @ 2-8 °C 3 days @ 2-8°C CK (Creatine Kinase) Sample Preparation and/or Centrifugation/ Instrument 4 weeks @ 2-8 °C Heparin Plasma (CEA) Cholesterol Special Transport Conditions or other Information 18-28°C = R.T. 2-8 °C = Refrigerated 22 days @ 2-8°C (CA) Carbamazepine Stability Physical Characteristics that Compromise Results As soon as possible store in 2-8°C Vitros Vitros Ice and pneumatic tube system Blood Gas Instruments Hemolysis or Turbidity Avoid evaporation Vitros R.T. Sensitive (CABG or CVBG) Cortisol 5 days @ 2-8 °C (ICORTA or ICORTP) Creatinine Serum Heparin or 0.1 mL 0.3 mL EDTA plasma (CREA) Creatinine Clearance Serum with Heparin 24 hour urine plasma (LCRP) Heparin or EDTA plasma Separate serum from cells within 4 hours after collection Indefinite if kept colder than -18°C 0.1 mL serum and 0.3 mL serum and 2 Serum: see above 0.5 mL urine mL urine Urine: 3 days @ R.T. The urine must be a 24 hour specimen and the serum must be drawn within 5 days of the urine collection. 5 days @ 2-8°C. (CRCL) Serum Return to 2-8°C as soon as possible 30 days @ 2-8 °C and 24 hour urine C-Reactive Protein (CRP) R.T. Sensitive 4 weeks if kept colder than – 20 °C 5 days @ R.T. 0.1 mL 0.3 mL Indefinite if kept colder than - 18°C 4 hours @ R.T. As soon as possible store in 2-8°C Separate serum from cells within 4 hours after collection 3 days @ 2-8 °C 6 months if kept colder than - 18°C 3 St. Luke's Health System Quick Guide Stability Chart Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred Cryoglobulins Acceptable Serum NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested 2.5 mL Serum 10 mL Gel is not acceptable Whole blood (CRYO) CSF Glucose (Cerebrospinal Fluid) R.T. Sensitive CSF 0.1 mL 0.3 mL Stability Physical Characteristics that Compromise Results Special Transport Conditions or other Information Sample Preparation and/or Centrifugation/ Instrument 18-28°C = R.T. 2-8 °C = Refrigerated Has to be kept @ 37°C for 1 hour until clotting is complete, separated from cells and kept at room temperature until tested. Patient needs to be fasting. Manual testing Tube needs to be put into 37°C for 1 hour until clotting is complete. Separate serum and hold at room Ref Lab only temperature until tested. 7 days @ 2-8 °C Store at 2-8 °C as soon as possible Run Stat Vitros Run Stat Vitros Trough specimen preferred Vitros - Ref Lab only 1 hour @ R.T. (CSFGLU) CSF Protein CSF 0.1 mL 0.3 mL 4 hours @ R.T. (Cerebrospinal Fluid) 3 days @ 2-8°C R.T. Sensitive (CSFPRO) Cyclosporine A R.T. Sensitive EDTA whole blood 0.5 mL 2 mL Serum only 0.1 mL 0.3 mL (LCSA) Digoxin R.T. Sensitive R.T. Sensitive Gel is acceptable Serum Heparin Plasma 0.1 mL 0.3 mL Gel tube not acceptable Serum 8 hours @ R.T. Vitros 7 days @ 2-8 °C 4 months if kept colder than -20°C 8 hours @ R.T. Serum tubes with Gel has shown decreased results Separate from cells within 4 hours after collection 7 days @ 2-8°C (LDIL) Electrolyte profile 6months @ colder than – 18 °C 8 hr@ R.T. 7 days @ 2-8°C (LDIG) Dilantin (Phenytoin) Turbidity and hemolyzed and xanthochromic samples falsely elevate results Heparinized plasma 0.4 mL 12 weeks @ colder than 18°C 24 hours @ R.T. 3 Hemolysis and TCO2 (ECO2) has to be prolonged contact days @ 2-8 °C kept in a tightly capped with cells increases K. tube until testing Individual test stability: NA: 4 days @ R.T. 7 days @ 2-8°C 1 mL (NA, K, CL, TCO2, AGAP) Vitros Separate from cells within 2 hours after collection Individual Test Handling: ECO2 and Cl: Separate from cells within 4 hours. ECO2 is R.T. Sensitive K: Separate from cells within 2 hours after collection. NA: Separate from cells within 2 days. K: 6 weeks @ R.T. or @ 2-8 °C CL: 7 days @ R.T. 4 weeks @ 2-8 °C Vitros ECO2: 24 hours@R.T. (LYTE) 3 days @ 2-8 °C (tightly capped) Estradiol Serum Heparin or 0.2 mL 0.5 mL 8 hours @ R.T. EDTA plasma R.T. Sensitive Hemolysis Avoid evaporation Lipemia Store in 2-8°C as soon as possible Turbidity Avoid Evaporation 2 days @ 2-8 °C (ESTRA) Ferritin Serum R.T. Sensitive (FERR) Fetal Hemoglobin APT Test (FETHGB) Folate Heparin plasma will show a negative 15% bias compared to serum and should not be used 0.2 mL 0.3 ml 4 weeks if kept colder than – 20 °C 8 hours @ R.T. Vitros - Ref Lab only Vitros - Ref Lab only Store @ 2-8 °C as soon as possible 5 days @ 2-8 °C 4 weeks if kept colder than -20°C ( cannot freeze Plasma ) Feces or Gastric Contents 0.5 mL 2 mL Serum 0.3 mL 0.6 mL Ran STAT Brown samples cannot be tested Specimen must be red in color Boise Lab only ≤24 hrs @ R.T. Turbidity Avoid Evaporation Vitros ≤ 7 days @ 2-8 °C Heparin not accepted Samples need to be due to frequent completely separated from inability to get results cellular material using plasma samples. ≤4 weeks if kept colder than – 20 °C Store @ 2-8°C as soon as possible 4 St. Luke's Health System Quick Guide Stability Chart Plasma or Serum Volume Folate Chemistry Test & (order code(s)) Specimen Type Preferred Free PSA (PSA, Free) R.T. Sensitive Acceptable Serum ONLY 0.3 mL mL of NOTE: For the minimum0.6 volume blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum 0.2 mL Requested 0.3 mL Stability Special Transport Conditions or other Information Sample Vitros Preparation and/or Centrifugation/ Instrument 18-28°C = R.T. 2-8 °C = Refrigerated 8 hrs @ R.T. 24hrs @ 2-8 °C (PSAF nonorderable result code) (PSAFT, IPSAR orderable codes) Physical Characteristics that Compromise Results longer @ colder than – 18 °C CANNOT ORDER JUST A FREE PSA. Must be ordered as the Free and Total battery(PSAFT) or as a Reflex to free(IPSAR). All samples are stable for 72 hours regardless of the presence or absense of ambient light. Separate from cells within 4 If reflex test (IPSAR) is hours after collection prior to ordered PSAF will be added if freezing serum. IPSA is within the range of 4.00-9.99. Immulite - Ref Lab only Serum Free T4 Serum ONLY 0.1 mL 0.3mL (T4, FREE) Serum Heparin or EDTA Plasma 0.2 mL 0.5 mL Serum Heparin Plasma 0.1 mL 0.3 mL Gel tube acceptable Serum (Gamma Glutamyl Transferase) (GGT) (GLU, GLU1HR, GLU2HR, GTT tests) HCG Quant, Serum (Serum HCG,Quant) R. T. Sensitive Heparin 0.1 mL 0.3 mL or EDTA plasma Serum Serum 8 hrs @ R.T. Turbidity Avoid Evaporation 4 weeks if colder than 20°C 2 days @ R.T. Remove serum/plasma from cells within 1 hour of collection Vitros 14 days if kept colder than -20 °C 7 days @ R.T. Remove from cellular material within 4 hours of collection 7 days @ 2-8 °C Heparin, EDTA, or Sodium fluoride plasma 0.1 mL Heparin, EDTA Plasma 0.3 mL 0.3 mL 2 months if kept colder than - 18°C 1 day @ R.T. Vitros lipemia ↑ avoid prolonged contact of serum with red cells Vitros Turbidity Avoid evaporation Separate serum from cellular material within 4 hours from collection. Vitros 7 days @ 2-8 °C I year if kept colder than 18° 0.6 mL 8 hours @ R.T. 5 days @ 2-8 °C see qualitative HCG under "serology testing" 4 weeks if kept colder than -20°C Vitros - Ref Lab only Store @ 2-8°C as soon as possible 7days @ 2-8 °C (LGENTP, LGENTT) Glucose Vitros - Ref Lab only Store at 2-8 °C as soon as possible 6 days @ 2-8 °C (LFSH) GGT Avoid Evaporation 4 weeks if kept colder than – 20 °C R.T. sensitive Gentamicin Turbidity 7 days @ 2-8 °C R.T. sensitive (IT4F) FSH(Follicle Stim Horm) 8 hrs @ R.T. As soon as possible put specimen into 2-8 °C. (BHCG) HDL (dHDL) Serum (HDL CHOLESTEROL) Heparin plasma 0.1 mL 0.3 mL 3 hours @ R.T. 3 days 2-8 °C Vitros R.T. Sensitive (LHDL) Hemoglobin A1C (Glycohemoglobin) (GHGB) Separate from cells within 3 hours of collection EDTA whole blood 0.5 mL 1 mL 3 weeks if kept colder than -20°C 24 hours @ R.T. Tosoh 14 days @ 2-8 °C 5 St. Luke's Health System Quick Guide Stability Chart Physical Characteristics that Compromise Results Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred H. pylori IgG Serum Acceptable Heparin NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested 0.3 mL 1mL 0.1 mL 0.3 mL (HPYLG) Serum (C-Reactive Protein, Highly Sensitive) Heparin Plasma Sample Preparation and/or Centrifugation/ Instrument 18-28°C = R.T. 2-8 °C = Refrigerated 1 day @ R.T. Immulite 3 days @ 2-8 °C Ref Lab only 4 hours @ R.T. Vitros 3 days @ 2-8 °C 6 months if kept colder than - 20°C R.T. sensitive (CRPHS) Hepatitis A Antibody, IgM Serum 0.1 mL 1 mL (HAVAB, IgM) 8 hours @ R.T. Not to exceed 3 hours on the Vitros - Ref Lab only system to avoid false results due to evaporation 7 days @ 2-8 °C Freeze at colder than 20°C if testing is not done within 7 days R.T. sensitive (LHAVM) Hepatitis A AB, Total Special Transport Conditions or other Information Plasma (Helicobacter Pylori, IGG) hsCRP Stability Serum 0.1 mL 1 mL - 8 hours @ R.T. Turbidity Not to exceed 3 hours on the Vitros - Ref Lab only system to avoid false results due to evaporation Turbidity Not to exceed 3 hours on the Vitros system to avoid false results due to evaporation Ref Lab only Turbidity Not to exceed 3 hours on the Vitros system to avoid false results due to evaporation (IgG and IgM) 5 days @ 2-8 °C R.T. sensitive 4 weeks if kept colder than - 20°C (LHAV) Hepatitis B Core Ab, Total Serum 0.1 mL 1 mL (IgG and IgM) 8 hours @ R.T. 48 hours @ 2-8°C R.T. sensitive (LHBCAB) Freeze at colder than 20°C if testing is not done within 48 hours Hepatitis B Core Ab, IgM Serum 0.1 mL 1 mL 7 days @ 2-8°C R.T. sensitive (LHBCM) Hepatitis B surface AB Ref Lab only 4 weeks @ - 20°C Serum 0.2 mL 1 mL (HBsAB) 8 hours @ R.T. Turbidity Not to exceed 3 hours on the Vitros system to avoid false results Ref Lab only due to evaporation Turbidity Not to exceed 3 hours on the Vitros - Ref Lab only system to avoid false results due to evaporation Turbidity Not to exceed 3 hours on the Vitros - Ref Lab only system to avoid false results due to evaporation 48 hours @ 2-8°C Freeze at colder than 20°C if testing is not done within 48 hours R.T. sensitive (LHBSAB) Hepatitis B surface AG 8 hours @ R.T. Serum 0.2 mL 1mL 8 hours @ R.T. (Hepatitis Bs Antigen) 48 hours @ 2-8°C (HBsAG) Freeze at colder than 20°C if testing is not done within 48 hours R.T. sensitive (LHBSAG) Hepatitis C Antibody Serum 0.1 mL 1 mL (HCV) 8 hours @ R.T. 48 hours @ 2-8°C Freeze at colder than 20°C if testing is not done within 48 hours R.T. sensitive (LHVC) HIV 1&2 Serum only (HIV 1/2 AB SCREEN W/RFX) R.T. sensitive Parent (original tube) required 0.5 mL 1 mL 8 hours @ R.T. Not to exceed 3 hours on instrument Vitros - Ref Lab only 48hours @ 2-8 °C Longer if kept colder @ 20 °C (LHIV) HIV Serum only Multispot Parent (original tube) required ( ) 0.4 mL 1 mL 2 days @ R.T. 7 days @ 2-8°C Long term if kept colder than -20°C Requires serum only and parent tube for HIV testing Manual kit Ref Lab only Homocysteine (HCY) Serum only Heparin or EDTA Plasma Tube has to be @ least 1/2 full to prevent negative bias 0.1 mL 0.5 mL 3 days @ R.T. 10 days @ 2-8°C 30 days if kept colder than - 20°C Sample must be put inice immediately after being drawn. All specimens can be kept on ice for up to 6 hours prior to centrifugation. At that time serum needs to be separated from cells and transferred to an aliquot tube. IgA Serum Heparin, EDTA plasma 0.1 mL 0.3 mL 1day @ R.T. Remove from cellular material within 2 hours after collection 6 St. Luke's Health System Quick Guide Stability Chart Physical Characteristics that Compromise Results Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred Serum NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Acceptable Minimum Requested Heparin, EDTA plasma 0.1 mL 0.3 mL Stability Special Transport Conditions or other Information 18-28°C = R.T. 2-8 °C = Refrigerated Sample Preparation and/or Centrifugation/ Instrument Remove from cellular material within 2 hours after collection 7 days @ 2-8 °C (IGA) IgG Serum Heparin, EDTA Plasma 0.1 mL 0.3 mL Serum Vitros - Ref Lab only Remove from cellular material within 2 hours after collection 7 days @ 2-8 °C (IGG) IgM 4 weeks if kept colder than - 18°C 1day @ R.T. Heparin, EDTA Plasma 0.1 mL 0.3 mL 4 weeks if kept colder than - 18°C 1day @ R.T. Vitros - Ref Lab only Remove from cellular material within 2 hours after collection 7 days @ 2-8 °C (IGM) Insulin Serum R.T. sensitive Heparin Plasma 1.0 mL 1.5 mL Heparinized Plasma Serum 0.1 mL 0.5 mL R.T. unacceptable (ICA for ABL analyzer, ICACOM for ISTAT) Iron * Serum (IRON) Iron/TIBC Heparin Plasma (CANNOT BE USED FOR TIBC) 0.1 mL 1 mL Serum ONLY 0.5 mL 2 mL with TIBC Sodium Fluoride (gray top) Heparin Plasma (on ice) Hemolysis ↓ 0.3 mL 1 mL R.T. sensitive for heparin tubes 7 days @ R.T. or 8°C if kept ANO2 4 days @ R.T. 2- keep capped, don't aliquot hemolysis ↑ 7 days @ 2-8 °C icteric sample ↓ 3 months @ colder than 18°C 4 days @ R.T. hemolysis ↑ (LACTIC) Serum Heparin Plasma 0.1 mL 0.3 mL (Lactic Dehydrogenase) Immulite icteric sample ↓ If Drawn with ABG can be ABL90 kept @ R.T. and tested within 10 minutes Serum must be from gel tube at least half full Do not use hemolyzed samples. i-Stat (cannot run serum on the i-Stat) Separate cells from serum within 4 hours Vitros Do not use hemolyzed samples. Separate cells from serum within 4 hours Vitros 3 months @ colder than 18°C Fluoride Oxalate Gray top has to be ½ Draw blood without using a stability: full or it will be tourniquet. Centrifuge and rejected remove from cells within 15 min of drawing. 8 hour @ R.T. Green top needs to 14 days @ 2-8 °C LDH Recommend overnight fast Ref Lab only 3 months @ colder than 18°C Plasma needs to be put Room temperature on ICE as soon as plasma is collected unacceptable 7 days @ 2-8 °C (IRONP) Lactic Acid Vitros - Ref Lab only 7 days @ 2-8 °C (INS) Ionized Calcium 4 weeks if kept colder than - 18°C 8 hours @ R.T. Vitros be on ice or it will be rejected Heparin needs to be run STAT 2 days @ R.T. hemolysis Refrigerated not recommended. Avoid prolonged contact with Separate from cells within 1 red cells hour after collection Do not use hemolyzed Vitros samples. (LDH) LDL (dLDL) Serum Heparin Plasma 0.1 mL 0.3 mL 1 day @ R.T. Separate from cells with 2 hours after collection Vitros 3 days @ 2-8 °C (LLDL) LH Serum (Luteinizing Hormone) Heparin, 0.2 mL 0.5 mL EDTA plasma 3 weeks if kept colder than -20 °C 5 days @ 2-8 °C 4 weeks if kept colder than -20 °C R.T. unacceptable Avoid evaporation Vitros Turbidity Return back to 2-8 °C as soon as possible Ref Lab only Samples are commonly drawn 12 hours after last dose of Lithium. Remove from cells within 4 hours of collection Vitros R.T. unacceptable (LLH) Lithium Serum EDTA plasma 0.1 mL 0.3 mL 1 day @ 2-8 °C R.T. Sensitive (LLI) Lipase 8 hours @ R.T. 6 months if kept colder than -18°C Serum Heparin plasma 0.1 mL 0.3 mL 1 week @ R.T. Gross lipemia Remove from cells as soon as possible Vitros hemolysis ↑ Vitros Turbidity or hemolysis Separate serum from cells with 2 hours 3 weeks @ 2-8 °C (LIPASE) Magnesium Serum Heparin plasma 0.1 mL 0.3 mL 1 week @ 2-8 ° C (MG) NT-pro BNP Serum Heparin or EDTA plasma 5 months if kept colder than -18 °C 1 week @ R.T. 0.4 mL 1 mL 1 month if kept colder than -18°C 3 days @ R.T 3 days @ 2-8°C 7 St. Luke's Health System Quick Guide Stability Chart Stability Physical Characteristics that Compromise Results 18-28°C = R.T. 2-8 °C = Refrigerated Turbidity or hemolysis Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Serum or Acceptable Preferred Heparin EDTA plasma NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below 0.4 mL Minimum 1 mL Requested 60 ųL 0.5 mL (PBNP) Osmolality (Serum) Special Transport Conditions or other Information 1 years @ -20° Serum Heparin plasma (OSM) Osmolality ( Urine) Urine (UOSM) Aliquot from 24 hour urine is perferred 60 ųL 0.5 mL Sample Preparation and/or Centrifugation/ Instrument Vitros - Ref Lab only 3 hours @ R.T. Keep specimen capped 3 days @ 2-8 ° C Keep @ 2-8 °C as much as possible prior to testing 7 days if kept colder than -20°C 3 hours @ R.T. Keep specimen capped 3 days @ 2-8 ° C Keep @ 2-8 °C as much as possible prior to testing 7 days if kept colder than -20°C Phenytoin (See Dilantin) (LDIL) Phosphorus Serum Heparin Plasma 0.1 mL 0.3 mL 3 days @ R.T. Separate from cellular material within 4 hours of collection. 7 days @ 2-8°C (PHOS) Prealbumin Serum Heparin Plasma 0.1 mL 0.3 mL 2 months if kept colder than -18°C ≤ 1 day @ R.T. Vitros Cannot test a previously frozen sample ≤ 14 days @ 2-8 C (PREALB) Separate from cellular material within 2 hours of collection. Vitros Ref Lab only Pro-BNP See NT-proBNP (PBNP) Procalcitonin Heparin Plasma Serum 0.2 mL 0.5 mL R.T. unacceptable 48 hours @ 2-8°C R.T. unacceptable 6 months if kept colder than - 25°C Need to run Stat 8 hours @ R.T. Turbidity The same type of sample MiniVidas must be used for subsequent testing on same patient. Reject specimen if not same type. (PCT) Prolactin Serum Heparin 0. 3mL 1mL Plasma R.T. sensitive (PROL) Protein electrophoresis &/or IFE, Serum (SPE, SPEPIF) Serum Only Immulite - Ref Lab only 7 days @ 2-8 °C 0.5 ml 1 ml 3 months if colder than – 20 °C 4 days @ R.T. Turbidity - not recommended, 2 weeks @ 2-8 °C 6 months if kept colder than - 18°C Quick Scan 2000 Ref Lab Only Gross Hemolysis 8 St. Luke's Health System Quick Guide Stability Chart Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred PSA, Total Acceptable Serum Only NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested 0.1 mL 0.3 mL R.T. sensitive Serum Heparin 1.0 mL 2 days @ 2-8 °C Serum or EDTA plasma 0.3 mL 1mL 4 weeks if kept colder than -20°c 2 days @ 2-8°C 4 weeks if kept colder than - 20°c R.T. unacceptable (ORPTH) Rheumatoid Factor Separate from cellular material within 4 hours after collection Serum Heparin Plasma Immulite R.T. unacceptable Put in 2-8°C as soon as possible after specimen is clotted and spun Vitros - Ref Lab only OR Specimens are run stat Avoid Evaporation 0.1 mL 0.3 mL Hemolysis R.T. unacceptable Turbidity Put back into 2-8 °C as soon as possible Hemolysis Vitros 1 day @ R.T. Separate serum/plasma from cells within 2 hours after collection 3 days @ 2-8 °C (RA) Rubella IgG Serum Heparin and EDTA Plasma 0.1 mL 0.3 mL (RUBTTR) Salicylate Heparin Plasma Serum Ref Lab only Turbidity (PTHCA) INTRAOPERATIVE PTH (The PTH ran during surgery) 8 hours @ R.T. Plasma Non Gel Heparin Plasma Sample Preparation and/or Centrifugation/ Instrument 18-28°C = R.T. 2-8 °C = Refrigerated Longer @ colder than – 20°C R.T. unacceptable PTH, Intact Special Transport Conditions or other Information 2 days @ 2-8 °C (PSASCR) -screen (IPSA) -diagnostic (IPSAR) -PSA with Reflex to PSA,FREE PTH, Intact & Calcium Stability Physical Characteristics that Compromise Results 0.1 mL 0.3 mL 6 months if kept colder than -20°C 1 day @ R.T. Vitros - Ref Lab only Turbidity 7 days @ 2-8 °C Store at 2-8 °C as soon as possible after clotting and spinning Store at 2-8°C as soon as possible Vitros - Ref Lab only 4 weeks if kept colder than - 20°C 7 days @ R.T. Gel is acceptable Separate from cells within 4 hours of collection Vitros 7 days @ 2-8 °C (SALI) Syphilis Screen (see Treponemal Antibody Screen) (SYP) Serum Heparin Plasma 0.2 mL 0.5 mL 6 months if colder than 18°C 1 day @ R.T. Immulite - Ref Lab only 3 days @ 2-8 °C Longer @ colder than – 18 °C T 3, Total or T4, Total Thyroxine See Total T3 or Total T4 Tacrolimus R.T. unacceptable Whole Blood EDTA 0.5 mL 2.0 mL 2 months if kept colder than - 70°C (LTACRO) Tegretol (see Carbamazepine) Theophylline Serum (LTHEO) Serum gel tubes are acceptable Thyroglobulin Serum R.T. sensitive Heparin Plasma 0.1 mL 0.3 mL Serum (Anti TPO AB) Heparin Plasma 0.2 mL Heparin Plasma 0.2 mL 0.5 mL 0.5 mL 60 days if kept colder than - 18°C 8 hrs @ R.T. 8 hours @ R.T. Vitros - Ref Lab only Specimen needs to be put into 2-8°C as soon as possible after collection Separate serum/plasma from cells within 4 hours after collection . Vitros PUT BACK INTO REFRIGERATOR ASAP Immulite - Ref Lab only Immulite Ref Lab only 2 days @ 2-8 °C (LTPOAB) Serum (LTOBT, LTOBP) Serum gel tubes are acceptable Total Protein Serum R.T. Sensitive Tube needs to be 1/3 full 7 days @ R.T. 3 days @ 2-8 °C 2 months at colder than 20°C R.T. Sensitive Tobramycin R.T. unacceptable 7 days @ 2-8 °C (TG) can only be ordered as part of the TGPNL panel Thyroid Peroxidase AB 7 days @ 2-8 °C Heparin or EDTA Plasma 0.1 mL Heparin plasma 0.1 mL 0.3 mL Longer is kept @ colder than - 20°C 2 hours @ R.T. Vitros 7 days @ 2-8 °C 0.3 mL 14 days if kept colder than -20°C 4 hours @ R.T. Separate serum/plasma from cells within 4 hours of collection 9 St. Luke's Health System Quick Guide Stability Chart Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred Serum R.T. Sensitive Acceptable Heparin plasma NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested 0.1 mL 0.3 mL Serum R.T. Sensitive Heparin Plasma 0.1 mL 0.3 mL Serum R.T. Sensitive Heparin Plasma 0.1 mL 0.3 mL Serum Heparin Plasma 0.2 mL 0.5 mL Serum Heparin plasma 0.1 mL 0.3 mL R.T. Sensitive Serum or heparinized plasma EDTA plasma 0.3 mL 1 mL Serum R.T. Sensitive Heparin Plasma 0.3 mL 1 mL Serum Heparin plasma 0.3 ml 1 ml Serum Heparin Plasma 0.5 mL 1 mL (URICA) Valproic Acid Vitros Store @ 2-8 °C as soon as possible Ref Lab only Avoid Evaporation Vitros - Ref Lab only Store @ 2-8 °C as soon as possible 4 weeks if kept colder than - 20°C 1 day @ R.T. Immulite - Ref Lab only Longer @ colder than – 18 °C 3 days @ R.T. Separate serum/plasma from cells within 4 hours of collection 6 months if kept colder than - 18°C 2 hours @ R.T. 4 weeks if kept colder than - 18°C 8 hours @ R.T. 4 weeks if kept colder than - 20°C 3 days @ R.T. 1 day @ R.T. 14 days @ 2-8 °C (LVALP) R.T. Sensitive Avoid Evaporation Vitros Turbidity Store at 2-8 °C as soon as possible after clotting and spinning Avoid evaporation Avoid Evaporation Vitros Vitros Store @ 2-8 °C as soon as possible lipemia ↓ Vitros 5 days @ 2-8 °C Serum Gel acceptable Vancomycin Turbidity 7 days @ 2-8 °C (ITSH) Uric Acid 4 weeks if kept colder than - 20°C 8 hours @ R.T. 7 days @ 2-8 °C (TROPI) TSH, 3rd Gen Turbidity 7 days @ 2-8 °C (LTRIG) Troponin I Vitros 3 days @ 2-8 °C (SYP) Triglyceride 6 months if kept colder than - 18°C 8 hours @ R.T. Sample Preparation and/or Centrifugation/ Instrument Separate serum/plasma from cells within 4 hours of collection 5 days @ 2-8 °C (IT4) Treponemal Antibody Screen (Syphilis Screen) 18-28°C = R.T. 2-8 °C = Refrigerated 7 days @ 2-8 °C (LT3) Total T4 Special Transport Conditions or other Information 3 days @ 2-8 °C (TP) Total T3 Stability Physical Characteristics that Compromise Results Valproic Acid samples should Vitros be monitored at a consistent time of day because concentration is affected by circadian rhythm. 14 days if kept colder than - 20°C Serum (foil) EDTA plasma Serum Gel Acceptable (foil) if protected from light 0.3 mL 1 mL 2 hours @ R.T. Separate serum/plasma from Vitros cells with 4 hours of collection 3 days @ 2-8 °C Light Sensitive (LVANCT, LVANCP) 14 days if kept colder than - 20°C Venous Blood Gases Heparinized R.T. Sensitive Whole blood 0.5 mL in a 3 mL syringe or 0.15 mL in a 1 mL syringe 0.65 mL i-Stat 30 minutes @ R.T. Blood gas instruments (VBG) 10 St. Luke's Health System Quick Guide Stability Chart Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred Vitamin B 12 R.T. unacceptable Acceptable Serum NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested 0.3 mL 1 mL Stability Physical Characteristics that Compromise Results Special Transport Conditions or other Information Sample Preparation and/or Centrifugation/ Instrument 18-28°C = R.T. 2-8 °C = Refrigerated 7 days @ 2-8 °C R.T. unacceptable Vitros 4 weeks if kept colder than - 20°C ONLY (B12) URINE TESTING Reducing Substance, Urine Urine Clinitest Urine 0.5 mL 10 mL 2 hours @ R.T. Clinitest Tablets 12 Hours @ 2-8 °C Manual testing 2 hours @ R.T. Manual kit R.T. Sensitive (URED) Urine HCG Screen First Morning Random Specimen Urine 0.1 1mL 48 hours @ 2-8°C (PREGU) Urine Macroscopic Dipstick testing Urine 0.1 mL 0.5 mL Longer if kept colder than -20°C 2 hours @ R.T Atlas Clinitek Manual reading of strips (UMAC,UMACCS,UROG) Urine Myoglobin Screen Urine R.T. Sensitive 0.5 mL for confirmation to Quest 5.0 mL 2 hours @ R.T. Atlas Clinitek (UMYO) Manual reading of strips See urine preservative chart URINE CHEMISTRY Urine Amylase Urine 0.3 mL 1.0 mL For 24 hours urine see collection information 7 days @ R.T. Method Vitros 30 days @2-8°C Urine Calcium Urine 0.3 mL 1 mL Frozen is unacceptable 5 days @ R.T. Vitros - Ref Lab only 5 weeks @ 2-8°C (UCA,UCAR) Urine Creatinine Urine 0.3 mL 1 mL 6 months is kept colder than - 18°C 3 days @ R.T. Vitros 5 days@ 2-8°C (UCR,UCRR,CRCL) Urine Glucose Urine 0.3 mL 1 mL 0.3 mL 1 mL Indefinite if kept colder than - 18°C R.T. unacceptable Need to analyze as soon after collection as possible Vitros R.T. Unacceptable (UGL, UGLR) Urine Magnesium (UMG, UMGR) Urine 1 week @ R.T. Vitros - Ref Lab only 1 week @ 2-8 °C 1 month if kept colder than -18°C 11 St. Luke's Health System Quick Guide Stability Chart Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred Acceptable NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested Stability Urine 0.3 ml 1 ml (MALR, MAL24) (for WRFM - MALBSQ) Urine Phosphorous Special Transport Conditions or other Information See urine preservative chart For 24 hours urine see collection information Sample Preparation and/or Centrifugation/ Instrument 18-28°C = R.T. 2-8 °C = Refrigerated URINE CHEMISTRY Urine Microalbumin Physical Characteristics that Compromise Results 1 day @ R.T. Method Vitros - Ref Lab only 7 days@ 2-8 °C Urine 0.3 ml 1 ml 6 hours @ R.T, Vitros - Ref Lab Only 2 days @ 2-8 °C (UPO4, UPOR) Urine Potassium Urine 0.3 ml 1 ml 6 months if kept colder than - 18°C 4 days @ R.T. Vitros 7 days @ 2-8 °C (UK,UKR) Urine Protein Electrophoresis &/or IFE Urine, random or 24 hour 10 ml 20 ml R.T. Sensitive Icludes Urine Total Protein (UPE, UPEPIF) Urine Sodium 6 months if kept colder than - 18°c 8 hours @ R.T, Quick Scan 2000 5 days @ 2-8 °C Ref Lab only 6 months if kept colder than - 18°C Urine 0.3 ml 1 ml 4 days @ R.T. Vitros 7 days @ 2-8 °C (UNA,UNAR) Urine Urea Nitrogen Urine 0.3 mL 1 mL R.T. unacceptable (UUN,UUNR,UNCL) Urine Uric Acid (UUA,UUAR) Urine 0.3 ml 1 ml 6 months if kept colder than - 18°c Refrigerate specimens that cannot be analyzed immediately 3 days @ R.T. R.T. unacceptable Vitros - Ref Lab only Need to be kept @ 2-8 °C Refrigerated or frozen urine samples Vitros - Ref Lab Only 12 St. Luke's Health System Quick Guide Stability Chart Plasma or Serum Volume Chemistry Test & (order code(s)) Specimen Type Preferred Acceptable NOTE: For the minimum volume of blood that needs to be collected draw double the minimum volume for plasma/serum listed below Minimum Requested 0.4 mL 0.5 mL Stability Physical Characteristics that Compromise Results Special Transport Conditions or other Information Sample Preparation and/or Centrifugation/ Instrument 18-28°C = R.T. 2-8 °C = Refrigerated Serology/Manual Tests Cryptococcal Antigen Serum CSF 6 months if kept colder than - 20°C 1 gm solid or 1 mL Approximate 2 gms 1 hour @ R.T liquid solid or 2 mL liquid 5 days @ 2-8°C Stool R.T. Sensitive (FECFAT) Stool (OCCBLD) Fecal Reducing Substance Stool Small amount to make thin smear 0.5 mL Once on card, 5 days 1.0 mL I hour @ R.T. Gastric contents 30 days if frozen at colder than -20°C 5 days Once on card Small amount to make thin smear (OCCGAS) Mononucleosis Screen Serum R.T. Sensitive (MONOSC) HIV-1/2 Rapid AB Serum HIV Screen (HIVEXP) HCG Serum /Urine Screen (BHCGSC,PREGU) Serum VDRL, Spinal Fluid CSF Heparinized or EDTA Plasma 0.1 mL Heparinized or EDTA plasma 0.1 mL Random urine 0.1 mL 0.5 mL 1.0 mL Urine first morning specimen (VDRLS) Serum Gastric contents have to be < Manual Testing 2 hours old prior to putting on card Manual Testing 24 hours @ 2-8°c 72 hours @ 2-8 °C 48 Hours @ 2-8°C Need to test Mother’s blood of infant < 9 months old Clear View Kit Manual Testing Manual Testing 48 hours if kept at colder than - 20°C 0.1 mL 1.0 mL (VDRL) R.T. sensitive Manual Testing 0.5 mL R.T. Sensitive VDRL, Serum Stool has to be < 2 hours old Manual Testing prior to be putting on card 5 days @ 2-8°C (RSST) Occult Blood, Gastric Manual test - Ref Lab only 30 days if frozen Fecal Occult Blood Gastric Occult Blood Manual test - Ref Lab only 13 days @ 2-8 °C (CRYPT) Fecal Fat, Qualitative Serum and CSF: 1.0 mL 2.0 mL 4 hours @ R.T. Manual Testing 4 days @ 2-8°C At the Ref Lab only 5 days if kept colder than - 20°C 4 hours @ R.T. Sent to State 4 days @ 2-8°C 5 days if kept colder than - 20°C 13