Photosynthesis Online Investigation -- CLASS COPY -BACKGROUND Photosynthesis is the process in which plants use sunlight to convert carbon dioxide and water into the simple sugar glucose and oxygen gas. The rate at which photosynthesis occurs is not always the same. The intensity of light, amount of carbon dioxide and water, and the availability of minerals are important factors that affect the rate of photosynthesis in plants. The rate also varies by species and a plant’s health and maturity. In this online investigation, an Elodea plant will be used to simulate the effect of light intensity and carbon dioxide concentration on the rate of photosynthesis. Photosynthesis rate will be measured by counting the number of bubbles produced at a particular light intensity and different carbon dioxide levels. Objective/Purpose: Determine the effect of light intensity and carbon dioxide concentration on the rate of photosynthesis. Procedure: 1. Go to the following website: www.newpathlearning.com/MML/PHOTOSYNTHESIS/files/BubbleLab.swf 2. Click on the prepare plant button. 3. Using your knowledge of light intensity and photosynthesis, determine the light intensity that would produce the most bubbles in the simulation. Use this light intensity to run the rest of the simulations. 4. Select 0 ppm for the CO2 concentration. 5. Click on the start button and count the number of bubbles produced in 5 minutes. (IMPORTANT: Note that the speed of the timer is stimulated). Record the number of bubbles in a data table. 6. Change the CO2 concentration to 300 ppm and repeat step 5. 7. Change the CO2 concentration to 600 ppm and repeat step 5. 8. Change the CO2 concentration to 1200 ppm and repeat step 5. 9. Share your group data with the class. NAME: _______________________________________ PERIOD: ______ DATE: _______________ Photosynthesis Online Investigation Pre-Lab Questions (Answer questions in complete sentences) 1. What does a plant need for photosynthesis to occur? 2. What factors can affect the rate of photosynthesis? 3. Why can the number of bubbles produced indicate the rate of photosynthesis? Hypothesis: (Hypothesis should relate to the purpose of the investigation in an “If…, then…. because statement) Observations: Light intensity that would produce the most bubbles in the simulation: Data Table 1: The effect of carbon dioxide levels on the rate of photosynthesis. Carbon Dioxide 0 ppm 300 ppm 600 ppm Concentration Number of Bubbles 1200 ppm Qualitative Data: Collect data from all of the groups in your class and organize it in a data table below. Data Table 2: Graph On a separate sheet of paper, create a LINE graph that represents the effect of carbon dioxide concentration on the rate of photosynthesis. STAPLE the graph paper to this lab before turning it in. Analysis 1. What conclusion can you draw about the rate of photosynthesis and its relationship to carbon dioxide concentration? EXPLAIN. Conclusion Does the data support your hypothesis? (Restate hypothesis in the answer) List data from simulation to support the above claim. Use what you have learned in class to explain WHY you got these results. What is the relevance of the findings from this experiment? (Why would this be useful to know?) What are some possible sources of error (mistakes that were made) that could have affected the outcome of the experiment? What are some other questions or factors about the rate of photosynthesis that you could test?