Grinspoon S, Corcoran C, Askari H, Schoenfeld D, Wolf L, Burrows B, Walsh M, Hayden D,
Parlman K, Anderson E, Basgoz N, Klibanski A 1998 Effects of androgen administration in
men with the AIDS wasting syndrome. A randomized, double-blind, placebo-controlled trial. Ann
Intern Med 129:18–26
ARTICLE
Effects of Androgen Administration in Men with
the AIDS Wasting Syndrome
A Randomized, Double-Blind, Placebo-Controlled Trial
Steven Grinspoon, MD; Colleen Corcoran, ANP; Hasan Askari, MBBS; David Schoenfeld, PhD;
Lisa Wolf, RN; Belton Burrows, MD; Mark Walsh, MS; Douglas Hayden, MA; Kristin Parlman,
MSPT; Ellen Anderson, MS, RD; Nesli Basgoz, MD; and Anne Klibanski, MD
1 July 1998 | Volume 129 Issue 1 | Pages 18-26
Background: Development of successful anabolic strategies to reverse the loss of lean body
mass is of critical importance to increase survival in men with the AIDS wasting syndrome.
Hypogonadism, an acquired endocrine deficiency state characterized by loss of testosterone,
occurs in more than half of all men with advanced HIV disease. It is unknown whether
testosterone deficiency contributes to the profound catabolic state and loss of lean body mass
associated with the AIDS wasting syndrome.
Objective: To investigate the effects of physiologic testosterone administration on body
composition, exercise functional capacity, and quality of life in androgen-deficient men with the
AIDS wasting syndrome.
Design: Randomized, double-blind, placebo-controlled study.
Setting: University medical center.
Patients: 51 HIV-positive men (age 42 ± 8 years) with wasting (body weight < 90% of ideal body
weight or weight loss > 10% of baseline weight) and a free testosterone level less than 42 pmol/L
(normal range for men 18 to 49 years of age, 42 to 121 pmol/L [12.0 to 35.0 pg/mL]).
Intervention: Patients were randomly assigned to receive testosterone enanthate, 300 mg, or
placebo intramuscularly every 3 weeks for 6 months.
Measurements: Change in fat-free mass was the primary end point. Secondary clinical end
points were weight, lean body mass, muscle mass, exercise functional capacity, and change in
perceived quality of life. Virologic variables were assessed by CD4 count and viral load.
Results: Compared with patients who received placebo, testosterone-treated patients gained fatfree mass ( –0.6 kg and 2.0 kg; P = 0.036), lean body mass (0.0 kg and 1.9 kg; P = 0.041), and
muscle mass ( –0.8 kg and 2.4 kg; P = 0.005). The changes in weight, fat mass, total-body water
content, and exercise functional capacity did not significantly differ between the groups. Patients
who received testosterone reported benefit from the treatment (P = 0.036), feeling better (P =
0.033), improved quality of life (P = 0.040), and improved appearance (P = 0.021). Testosterone
was well tolerated in all patients.
Conclusions: Physiologic testosterone administration increases lean body mass and improves
quality of life among androgen-deficient men with the AIDS wasting syndrome.
The AIDS wasting syndrome is characterized by loss of lean body mass out of proportion to
weight [1, 2]. The few effective treatments that have been identified are short-term pharmacologic
agonists. Because loss of lean body mass is associated with decreased survival in men with the
AIDS wasting syndrome [3], development of therapeutic strategies to increase lean body mass is
of critical importance. Half of all men with AIDS are hypogonadal [4], and serum androgen levels
correlate with lean body mass among hypogonadal men with the AIDS wasting syndrome [5].
Previous studies in non-HIV-infected hypogonadal men show that androgen administration has a
significant anabolic effect on body composition [6-10].
We hypothesized that loss of the potent anabolic hormone testosterone in men with the AIDS
wasting syndrome may contribute to the critical loss of lean body mass. Therefore, we
investigated the effects of physiologic testosterone administration in men with the AIDS wasting
syndrome.
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Methods
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Methods
Patients
In 1995 and 1996, 51 HIV-positive men (42 ± 8 years of age) were recruited from the
multidisciplinary HIV practice at the Massachusetts General Hospital and from newspaper,
television, and radio advertisements. Weight, testosterone levels, and medication history were
determined at a screening assessment. To be included in the study, patients had to have
decreased free testosterone levels, defined as less than 42 pmol/L at screening (normal range for
men 18 to 49 years of age, 42 to 121 pmol/L [12.0 to 35.0 pg/mL]), and wasting, defined as
weight less than 90% of ideal body weight or involuntary weight loss greater than 10% of baseline
weight [11]. The CD4 count was not an inclusion criterion. We excluded patients with severe
diarrhea (>6 stools/d); hemoglobin value less than 5.0 mmol/L (<8 g/dL); platelet count less than
50 000 cells/mm3; creatinine concentration greater than 177 µmol/L (>2 mg/dL); new opportunistic
infection within 6 weeks of screening; use of testosterone, anabolic steroids, growth hormone,
ketoconazole, or systemic steroid therapy within 3 months before screening; or history of prostate
cancer. In addition, patients receiving antiretroviral agents, including protease inhibitors, were
required to be receiving a stable regimen for at least 6 weeks before study entry. Ten patients
were receiving long-term, stable therapy with megestrol acetate for at least 8 weeks before study
entry and were equally distributed between the two treatment groups (5 in the testosterone group
and 5 in the placebo group). All patients gave written consent, and the study was approved by the
Human Studies Committee of the Massachusetts General Hospital.
Protocol
Patients were randomly assigned to receive testosterone enanthate, 300 mg (Bio-Technology
General Corp., Iselin, New Jersey), or placebo intramuscularly every 3 weeks by self-injection.
Participants were stratified for weight less than or greater than 90% of ideal body weight and
megestrol acetate use before randomization. Randomization was performed by the
Massachusetts General Hospital Pharmacy by using a permuted block algorithm. The
correspondence between patient code number and drug was generated by the study statistician;
this list was available to the hospital pharmacist but not to the investigators or patients. The
placebo contained sesame oil with chlorobutanol as a preservative and matched testosterone
enanthate in color and consistency. The study drug was bottled by the Massachusetts General
Hospital Pharmacy in containers labeled with the study name, expiration date, and patient code.
Before the first injection, participants returned within approximately 2 weeks of the screening visit
for a 3-day baseline inpatient visit to the General Clinical Research Center at the Massachusetts
General Hospital for hormonal, nutritional, immune function, and body composition analysis,
which included assessment by dual-energy x-ray absorptiometry, bioimpedance analysis,
potassium-4040 K) isotope analysis, and measurement of urinary creatinine excretion. No patient
experienced the onset of a new opportunistic infection, other complication, or substantial weight
change between the screening and baseline visits. Patients were instructed on the proper
technique for intramuscular injection; those who were unable to self-administer the study drug
received injections every 3 weeks from the nursing staff of the General Clinical Research Center.
Patients returned for an outpatient visit at 3 months for assessment of weight and determination
of total-body potassium content and for a 3-day inpatient visit at 6 months; this visit was identical
to the baseline evaluation. Patients also reported on response to therapy at the 6-month visit.
Baseline data from 26 patients have been reported elsewhere [5]. Subsequent study visits were
timed to correspond to the midpoint between study drug injections. Study drug compliance was
confirmed by history, medication diaries, outpatient injection records, empty vial counts, and
serum testosterone levels. History of medication use was assessed at each visit. The change in
fat-free mass assessed by dual-energy x-ray absorptiometry was the primary clinical end point;
changes in weight, muscle mass, total body potassium content, and quality of life were secondary
end points.
Body Composition Analysis
Body composition was determined by four methods: 1) dual-energy x-ray absorptiometry to
assess fat and fat-free mass (Hologic-2000 densitometer, Hologic, Inc., Waltham, Massachusetts;
precision error, 3% for fat and 1.5% for fat-free mass [12], 2) 40K isotope analysis to assess totalbody potassium content in a whole-body counter with sodium iodide detectors fixed above and
below the patient at the xiphoid level (Canberra Nuclear, Meriden, Connecticut; precision error <
2.5% on the basis of repeated calibration with a known potassium chloride source [Appendix]), 3)
urinary creatinine excretion averaged over 3 days (during which the patient received a meat-free
diet) multiplied by a constant of 18 kg of muscle per gram of urinary creatinine and indexed for
height to determine the percentage of predicted muscle mass [13, 14], and 4) bioimpedance
analysis to determine total-body water content (Bioelectrical Impedance Analyzer Model BIA-101,
RJL Systems, Clinton Turnpike, Michigan; correlation with deuterium oxide equivalent to R = 0.99
[15]). Lean body mass was derived from total-body potassium content by using the Equation of
Forbes and Lewis of 68.1 mEq of potassium per kg of lean body mass [16].
Nutritional Assessment
Weight was measured on the first day of each visit after an overnight fast. The percentage of
ideal body weight was calculated on the basis of standard height and weight tables [17]. Patients
were instructed on completion of a 4-day food record, which was analyzed for total calorie, fat,
protein, and carbohydrate content (Minnesota Nutrition Data Systems, version 8A/2.6,
Minneapolis, Minnesota) by the Clinical Research Center dietitian. Patients received an isocaloric,
meat-free, protein-substituted diet 3 days before and during the inpatient assessments at baseline
and at 6 months, during which creatinine excretion and nitrogen balance were measured. Total
urinary nitrogen excretion was measured by the Kjeldal technique from consecutive 24-hour
collections averaged over 3 days. Nonurinary nitrogen losses were assumed to be constant at 4
g/d [13, 18, 19]. Nitrogen intake was derived from total protein intake divided by a constant of
6.25 g of protein per g of nitrogen [19]. Calorie and protein intake were monitored on a daily basis
and were modified to match the reports in the outpatient food records immediately before these
visits. Resting energy expenditure was measured by indirect calorimetry with a metabolic cart.
Energy requirements were calculated by using the Harris-Benedict equation [20].
Patient Reports of Response to Therapy
Each patient's perceived well-being was assessed at the end of the study by using nine linear
analogue-scale questions on the overall treatment effect, change in quality of life, personal
appearance, weight, and appetite (Table 1) [21]. A Karnofsky score was also determined at each
visit.
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Table 1. Assessment of Patient Response to Therapy*
Exercise Functional Testing
Exercise history was assessed by a standardized questionnaire adapted from the study by Kohl
and coworkers [22]. Exercise functional status was determined at the baseline and final visits by
the physical therapy department of the Massachusetts General Hospital by using the 6-minute
walk test, the timed sit-to-stand test, and the timed get-up-and-go test [23-25]. The distance
covered in 6 minutes, the number of times the patient was able to move from a sitting to standing
position in 10 seconds, and the time to cover a distance of 3 meters after standing from a seated
position was recorded for each patient.
Biochemical and Immunologic Assays
Hematocrit and serum levels of follicle-stimulating hormone, luteinizing hormone, sex hormonebinding globulin, and prolactin were measured at the baseline and final visits by using published
methods [26]. Serum levels of total and free testosterone were measured by radioimmunoassay
kit (Diagnostics Products Corp., Los Angeles, California) with intra-assay coefficients of variation
of 5% to 12% for total testosterone and 3.2% to 4.3% for free testosterone. CD4 cell counts were
measured by flow cytometry (Becton Dickinson Immunocytochemistry Systems, San Jose,
California). Viral burden was determined by using the Amplicor HIV-1 monitor test (Roche
Molecular Systems, Branchburg, New Jersey).
Statistical Analysis
Sample size was based on the change in lean body mass in response to testosterone
administration among adult men with acquired hypogonadism [8]. A change of 3.2% ± 4.0% was
expected over 6 months. With 20 patients in each group, the study had an 80% chance of seeing
an effect of testosterone at a two-sided P value of 0.05 if the true difference in lean body mass
was 3.2% between the groups. Because some drop-out was expected, enrollment was planned
for 50 patients to ensure that 20 evaluable patients would remain in each randomization group.
Baseline comparisons between groups were performed by using t-tests for continuous variables
and the Fisher exact test for categorical variables. The treatment effect over 6 months was
estimated by analysis of covariance with all observations available from each patient in an
intention-to-treat analysis.
Forty-one patients completed the protocol (Figure 1). No follow-up data were available from eight
patients. Partial follow-up data were available from two patients. In this model, we compared the
change from baseline at 6 months in the two treatment groups while controlling for the baseline
value. We used a t-test to compare patient reports of response to therapy at 6 months between
the groups. Patients were classified according to whether they were receiving protease inhibitors
at baseline, started receiving them during the study, or never received them. We used the Fisher
exact test to compare the proportions in these three groups between the two treatment and to
compare survival between groups. To ensure that the differences in the primary end points were
not biased by mortality, we did a secondary analysis that combined mortality and body
composition. In the combined analysis, we classified deaths as having an extreme decrease in
mass, then used a Wilcoxon test to compare the groups. The change in body composition
variables was compared between users and nonusers of protease inhibitors by using the Student
t-test.
Figure 1. Flow of participants through the trial. R
= randomization.
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Methods
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Discussion
Author & Article Info
References
Results
Clinical Characteristics
Clinical characteristics of the study sample are shown in Table 2. Age, weight, body composition,
Karnofsky score, virologic variables, and screening testosterone levels did not significantly differ
between the testosterone and placebo groups. Except for serum albumin level, nutritional status
was the same between the groups at baseline. All patients had a decreased serum level of free
testosterone at screening and therefore met the entrance criterion for the study. Serum
testosterone levels, repeatedly measured at baseline for subsequent comparison with end-ofstudy values, were not below the normal range in any patient. Testosterone levels were the same
among patients who completed the study and were included in the treatment analysis (Table 3).
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Table 2. Clinical Characteristics of the Study Sample*
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Table 3. Clinical Response to Treatment*
Hormone Levels
Serum levels of total and free testosterone increased significantly, whereas serum levels of sex
hormone-binding globulin and follicle-stimulating hormone decreased significantly in the
testosterone group compared with the placebo group (Table 3).
Body Composition
Compared with patients who received placebo, testosterone-treated patients gained fat-free mass
(change, –0.6 kg and 2.0 kg; P = 0.036), lean body mass (change, 0.0 kg and 1.9 kg; P = 0.041),
and muscle mass (change, –0.8 kg and 2.4 kg; P = 0.005) (Figure 2, Table 3). The change in
total-body potassium content from which lean body mass was derived was also significantly
greater in the testosterone group (P = 0.041; data not shown). The changes in fat mass and totalbody water content did not differ (Table 3). Among testosterone-treated patients, a highly
significant correlation was found between the gain in fat-free mass as assessed by dual energy xray absorptiometry and the gains in lean body mass as assessed by the 40K-isotope technique (r =
0.65; P = 0.001) and muscle mass as assessed by urinary creatinine excretion (r = 0.56; P =
0.009). Use of protease inhibitor was not associated with a gain in lean body mass in either
treatment group. In the testosterone group, the 10 patients who were not taking protease
inhibitors had a more significant gain in fat-free mass than the 11 patients who were taking these
drugs (3.5 ± 2.8 kg and 0.6 ± 2.9 kg; P = 0.034).
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Figure 2. Mean change ± SE for fat-free
mass assessed by dual-energy x-ray
absorptiometry, lean body mass
determined from total-body potassium
content, and muscle mass from urinary
creatinine excretion in patients who
received testosterone (left) and patients
who received placebo (right) over 6
months. * P < 0.05 for the change from
baseline between the testosterone group and
placebo group by analysis of covariance. ** P
<0.01 for the change from baseline between
the testosterone group and placebo group by
analysis of covariance. n = number of patients
for whom paired baseline and end-of-study
data are available.
Nutritional Status
Patients who received testosterone gained an average of 1.9 kg more than patients who received
placebo, but this difference was not statistically significant. Among the 21 testosterone-treated
patients in whom paired measurements of dual-energy x-ray absorptiometry and weight were
made, weight increased an average of 2.5 kg; this increase was similar to the increase in fat-free
mass in this group (2.9 kg). Changes in other nutritional variables did not differ significantly
between the groups (Table 3).
Patient Reports of Response to Therapy
Significant differences between the groups were noted in the responses to six of nine patient
reports of response to therapy (Table 1). Testosterone-treated patients reported overall benefit
from treatment (P = 0.036), feeling better (P = 0.033), improved quality of life (P = 0.040), and
improved appearance (P = 0.021) compared with patients who received placebo. The two groups
had similar Karnofsky scores.
Exercise Functional Testing
Changes in exercise performance did not significantly differ between groups (Table 3).
Virologic and Hematologic Values
The changes in viral load, CD4 count, and hematocrit did not significantly differ between the
groups (Table 3). Viral load decreased significantly in the testosterone group (P = 0.027) and the
placebo group (P = 0.031) (Table 3). A similar proportion of patients in each group was receiving
protease inhibitors at baseline (19% of patients who received testosterone and 16% of patients
who received placebo) and started receiving these drugs during the study (27% of patients who
received testosterone and 31% of patients who received placebo).
Compliance, Side Effects, and Adverse Events
All patients claimed compliance with the study; this was confirmed by medication diary, injection
records, and empty vial count. Seven patients died of AIDS-related complications before
completing the study (three in the testosterone group and four in the placebo group). No
difference in survival was noted between the groups. Results of the secondary analyses that
combined mortality with fat-free mass, muscle mass, and body cell mass were similar to the
results of primary analyses of these variables alone (P = 0.04, 0.03, and 0.07 for fat-free mass,
muscle mass, and lean body mass, respectively). Testosterone was well tolerated in all patients.
Two patients (one in each group) dropped out of the study for personal reasons. One additional
patient was withdrawn from the study at baseline before initiation of therapy because of
gastrointestinal bleeding. No patient withdrew from the study because of a drug-related side
effect. Prostate enlargement or new nodules were not detected in any patient.
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Methods
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Discussion
Author & Article Info
References
Discussion
The development of successful anabolic strategies to increase lean body mass is critical in men
with the AIDS wasting syndrome, in whom the decrease in lean body mass is associated with
decreased survival [3]. Previous short-term pharmacologic therapies have had limited efficacy in
increasing lean body mass. Hypogonadism, an acquired endocrine deficiency state, was
recognized recently to be highly prevalent among men with the AIDS wasting syndrome and
results in loss of the potent endogenous anabolic factor testosterone.
We assessed the effects of testosterone administration in men with the AIDS wasting syndrome
and decreased free testosterone levels. Because of the known variability in free testosterone
levels and regression toward the mean, some patients had a level within the normal range on
baseline retesting. Thus, some patients may have had only relative androgen deficiency. Data
obtained from our sample of HIV-infected men show that testosterone administration substantially
increases lean body mass and improves patient well-being.
The anabolic effect of testosterone was shown by the significant increase in fat-free mass in
patients in the testosterone group compared with patients in the placebo group. Testosterone
treatment also increased total-body potassium content, a sensitive index of lean body mass that is
lost out of proportion to fat mass and weight among men with the AIDS wasting syndrome [1, 2].
At baseline, patients weighed, on average, 94% of ideal body weight but had only 75% of
predicted muscle mass, suggesting a great need for anabolic therapy in this population. Of note,
the gain in lean body mass was not attributable to protease inhibitor use or to a difference in
disease status between the groups.
A limitation of our study was that we were not able to collect follow-up data on eight patients
because of death (seven patients) or discontinuation at baseline related to underlying morbidity
(one patient). However, partial data on two additional patients were available and were included
in the intention-to-treat analysis.
Patients who received testosterone gained 1.9 kg in body weight compared with patients who
received placebo, but this change was not statistically significant. The changes in total-body water
and fat content also did not differ significantly between the treatment groups. The absence of any
treatment effect on water or fat content suggests that the effect of testosterone is primarily
anabolic. The more substantial effect of testosterone on lean body mass than on weight is
consistent with the known anabolic effects of testosterone in androgen-deficient men [8, 27]. The
mechanism of androgen effects on fat-free mass and muscle mass in this population is not
known. In the two groups, caloric intake did not increase and nitrogen balance did not differ.
Physiologic androgen replacement has been shown to directly affect muscle protein synthesis in
some studies of non-HIV-infected hypogonadal men [7, 10]; further studies are therefore needed
to determine the mechanism of androgen action in this population. Although we found no effect of
testosterone on exercise functional capacity, we did not strictly control for exercise, and patients
with advanced HIV disease may not have been able to attain their maximum performance on
effort-dependent testing.
The mechanisms of androgen deficiency in HIV-infected men are not known but may relate to
decreased gonadotropin production caused by severe illness [28, 29]; undernutrition [30]; or the
effect of medications, such as megestrol acetate [31]. Gonadotropin levels were elevated in 25%
of patients, consistent with primary gonadal failure; the remaining patients had normal or low
gonadotropin levels. Prolactin levels were elevated in 16% of patients at baseline and may have
contributed to hypogonadism in some patients. None of the patients had clinical evidence of
hemochromatosis or other diseases that might result in hypogonadotropic hypogonadism.
Pituitary imaging was not performed unless it was clinically indicated for symptoms of mass effect.
No differential effect of androgen replacement was found in men with primary or secondary
hypogonadism.
An important result of this study is that patients reported benefit from testosterone administration.
Previous studies documented reduced quality of life among symptomatic patients with AIDS,
including decreased physical function, role function, social function, and mental health perception
and increased pain [32]. Rabkin and Rabkin [33] showed that 80% of hypogonadal men reported
decreased energy. We chose to measure patients' perceived benefits in response to treatment by
using a standardized questionnaire (Table 1) that assessed appetite, image, weight, overall wellbeing, quality of life, and benefit of therapy. This instrument has been used previously to assess
treatment effects in men with the AIDS wasting syndrome [21]. Patients who received
testosterone reported an overall benefit from therapy and improvements in overall well-being,
quality of life, and concern about weight and appearance compared with patients who received
placebo. Positive trends toward improvement in appetite and enjoyment of eating were also noted
but were not statistically significant. These data suggest a true benefit with respect to quality of life
in testosterone-treated patients. However, further studies that use newly developed
questionnaires with more firmly established psychometric properties are needed to more
thoroughly investigate the effects of testosterone on quality of life in this population.
Few therapies have proven successful in the treatment of the AIDS wasting syndrome. These
therapies have used primarily pharmacologic methods to influence body composition and
appetite. In randomized, controlled studies, therapy with growth hormone or megestrol acetate
had substantial effects on nutritional and body composition values. However, the rationale for the
use of these agents differs greatly from that for the use of testosterone in our study. Megestrol
acetate is used primarily as an appetite stimulant to increase caloric intake and weight but not
lean body mass [21, 34]. Growth hormone administration at pharmacologic doses is also anabolic
and increases fat-free mass as well as weight but has not been shown to improve quality of life
[35]. Other anabolic agents, including the synthetic oral androgens, are currently being studied but
may be associated with increased risk for liver toxicity.
The anabolic effects of testosterone were achieved without reported side effects in any patient.
Testosterone was given at a dosage of 300 mg intramuscularly every 3 weeks, a standard
replacement dosage for androgen-deficient men [36]. Although pharmacologic levels may have
been reached immediately after administration in some of the study patients, the mean
testosterone level of the treated patients, measured on day 10 of the 21-day injection cycle, was
in the normal reference range. This suggests that the observed results were consistent with
physiologic androgen administration and not with sustained pharmacologic therapy.
Our study does not investigate testosterone administration for women with the AIDS wasting
syndrome. Women constitute an increasing percentage of HIV-infected patients [37] in whom
testosterone levels are decreased, and these levels have been shown to correlate with indices of
muscle mass [38]. Transdermal delivery of testosterone to women with AIDS wasting was
recently shown to be safe and was not associated with side effects during short-term
administration [39]. Determination of the clinical efficacy of testosterone administration in women
with the AIDS wasting syndrome is therefore an important area of future research.
Our data suggest that men with the AIDS wasting syndrome may benefit from screening for
androgen deficiency, and, if they are androgen deficient, should receive testosterone replacement
to increase lean body mass and improve their sense of well-being. For patients with low-to-normal
free testosterone levels, repeated testing may provide sufficient evidence for androgen deficiency
to justify treatment. We cannot generalize our results to patients with borderline low levels of free
testosterone because all patients in our study had a low free testosterone level on screening.
Further studies are needed to address the critical issue of whether testosterone administration is
effective in men with persistently borderline or normal serum androgen levels and in women.
Appendix: Potassium-40 Counting
The total 40K count was corrected for simultaneous radon measurement before unblinding by
using regression equations developed independently for the upper and lower sodium iodide
detectors from 68 consecutive analyses performed over 72 hours on a calibrated standard. For
the upper detector, the change in 40K attributable to radon was calculated as follows:
Change 40K counts = (0.282 x radon count at 1765 keV) + 0.001 (r2 = 0.49; P < 0.001)
For the lower detector, the change in
40K
attributable to radon was calculated as follows:
Change 40K counts = (0.285 x radon count at 1765 keV) + 0.001 (r2 = 0.75; P < 0.001)
The 40K count at each detector for each scan was summed to obtain the total 40K count. Totalbody potassium content was derived from a constant of 95 g of potassium per 13 800 counts of
potassium determined in a calibrated phantom.
From Massachusetts General Hospital, Harvard Medical School, Boston Veterans Affairs Medical
Center, and Boston University Medical Center, Boston, Massachusetts.
Dr. Askari: Department of Endocrinology, Diabetes and Metabolism, Washington University
School of Medicine, 660 South Euclid Avenue, Box 8127, St. Louis, MO 63110-1093.
Dr. Schoenfeld, Mr. Hayden, and Ms. Anderson: Biostatistics and Nutrition Centers, General
Clinical Research Center, White 13, Massachusetts General Hospital, 55 Fruit Street, Boston, MA
02114.
Ms. Wolf: Joslin Diabetes Center, 1 Joslin Place, Boston, MA 02215.
Dr. Burrows: Radiology Department, Mail Route Stop 114, Boston Veterans Administration
Medical Center, 150 South Huntington Avenue, Boston, MA 02130.
Mr. Walsh: Radiation Safety Unit, Mail Route Stop 11, Boston Veterans Administration Medical
Center, 150 South Huntington Avenue, Boston, MA 02130.
Ms. Parlman: Physical Therapy Department, Wang Ambulatory Care Center 128, Massachusetts
General Hospital, 55 Fruit Street, Boston, MA 02114.
Dr. Basgoz: Infectious Disease Unit, Founders 8, Massachusetts General Hospital, 55 Fruit
Street, Boston, MA 02114.
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Author and Article Information
For author affiliations and current author addresses, see end of text.
Acknowledgments: The authors thank Martin Hirsch, MD, for continued support; Benjamin
Davis, MD, Howard Heller, MD, and John Doweiko, MD, for help with patient care and
recruitment; Andrew Guccione, PhD, Gregory Newbauer, BA, Madeline Costello, PT, Judy
Krempin, BS, Maxine Sleeper, BA, and Kristen Lee, BS, for technical assistance; and Karen
Hopcia, RN, and the nursing and nutrition staffs of the Massachusetts General Hospital General
Clinical Research Center for dedicated patient care.
Grant Support: In part by National Institutes of Health grants R01-DK49302, MO1-RR01066,
P32-DK07028, and F32-DK09218.
Requests for Reprints: Steven Grinspoon, MD, Neuroendocrine Unit, Bulfinch 457B,
Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114.
Current Author Addresses: Drs. Grinspoon and Klibanski and Ms. Corcoran: Neuroendocrine
Unit, Bulfinch 457B, Massachusetts General Hospital, 55 Fruit Street, Boston, MA 02114.
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References
References
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