North Carolina State University is a landGrant university and a constituent institution Department of Forest Biomaterials of The University of North Carolina NC STATE UNIVERSITY College of Natural Resources Campus Box 8005 Raleigh, NC 27695-8005 919.515.5807 919.515.6302 (fax) Paper Science & Engineering Wood Products WP Extension 919.515.2888 919.515.3181 919.515.5637 STANDARD OPERATING PROCEDURE (SOP) FOR HANDLING LIQUID NITROGEN Location: 3225A Pulp and Paper Labs; 1235 & 1235A Pulp and Paper Labs Contact: Barbara White, Research Operations Manager Telephone: 919-513-0298 or 919-753-8523 CAUTIONS ● ● FROSTBITE HAZARD. Liquid nitrogen boils at -196° C. During operation, components of this equipment become very cold. Contact with unprotected skin can result in severe burns. DO NOT begin this procedure without wearing appropriate personal protective equipment (PPE). ASPHYXIATION HAZARD. Operation of this equipment liberates large quantities of nitrogen gas. There is an asphyxiation hazard due to displacement of oxygen. Take appropriate measures to ventilate the space and stand away from the unit during liquid nitrogen transfer. [D:\533574135.doc] [D:\533574135.doc] Standard Operating Procedure 1. Review the MSDS information before handling liquid nitrogen. 2. When handling liquid nitrogen, observe the following safe procedures: i. Wear safety goggles. ii. Wear gloves which are designed to protect the hands from extreme cold. iii. Perform any task with adequate ventilation. When transferring liquid nitrogen from a commercial tank to dewars, observe the following safe procedures: i. Attach the appropriate cryogenic delivery tube to the liquid connection of the commercial tank. ii. Open the liquid valve slowly. iii. DO NOT overfill the Dewar. iv. Close the liquid valve when finished. v. Remove the cryogenic delivery tube from the dewar and disconnect it from the commercial tank, avoiding contact with the skin. vi. Insert the liquid withdrawal device into the Lab 50 Dewar slowly to avoid spattering liquid nitrogen. [D:\533574135.doc]