GE Healthcare
Life Sciences
Fraction collector
F9-C and F9-R
Operating instructions
Original instructions
Table of Contents
Table of Contents
1
2
3
4
5
6
Introduction ..........................................................................................................
5
1.1
1.2
1.3
About this manual ................................................................................................................................
Important user information .............................................................................................................
Regulatory information ......................................................................................................................
6
7
9
Safety instructions ...............................................................................................
12
2.1
2.2
2.3
2.4
Safety precautions ...............................................................................................................................
Labels .........................................................................................................................................................
Emergency procedures ......................................................................................................................
Recycling information .........................................................................................................................
13
20
23
25
Fraction collector overview ...............................................................................
26
3.1
Fraction collector F9-C ......................................................................................................................
3.1.1
Function ..................................................................................................................................................
3.1.2
Fraction collector F9-C illustrations ............................................................................................
3.1.3
Cassettes, Cassette tray and racks ............................................................................................
3.2
Fraction collector F9-R ......................................................................................................................
3.2.1
Function ..................................................................................................................................................
3.2.2
Fraction collector F9-R illustrations ............................................................................................
3.2.3
Tubes .......................................................................................................................................................
27
28
29
33
37
38
39
42
Installation ............................................................................................................
44
4.1
4.1.1
4.1.2
4.1.3
4.2
4.2.1
4.2.2
4.3
4.4
4.5
Room and site requirements ...........................................................................................................
Delivery and storage .........................................................................................................................
Placement and space requirements ..........................................................................................
Site requirements ...............................................................................................................................
Connect to ÄKTA pure .........................................................................................................................
Power and communication ...........................................................................................................
Connect tubing ....................................................................................................................................
Prepare waste tubing for Fraction collector F9-C .................................................................
Set the delay volume ..........................................................................................................................
Performance test ..................................................................................................................................
45
46
47
50
51
52
58
60
62
64
Operation ..............................................................................................................
65
5.1
5.2
Fraction collector F9-C .......................................................................................................................
Fraction collector F9-R .......................................................................................................................
66
73
Maintenance .........................................................................................................
80
6.1
6.2
81
83
Fraction collector F9-C .......................................................................................................................
Fraction collector F9-R .......................................................................................................................
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
3
Table of Contents
7
Reference information ........................................................................................
84
A
Declaration of Hazardous Substances (DoHS) ................................................
85
Index .......................................................................................................................
88
4
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
1 Introduction
1
Introduction
About this chapter
This chapter contains important user information, description of safety notices, regulatory information and a general description of the intended use of Fraction collector F9-C
and Fraction collector F9-R.
In this chapter
This chapter contains the following sections:
Section
See page
1.1 About this manual
6
1.2 Important user information
7
1.3 Regulatory information
9
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
5
1 Introduction
1.1 About this manual
1.1
About this manual
Purpose of this document
The Operating Instructions provide you with the instructions needed to install, operate
and maintain Fraction collector F9-C and Fraction collector F9-R in a safe way.
Typographical conventions
Software items are identified in the text by bold italic text. A colon separates menu levels,
thus File:Open refers to the Open command in the File menu.
Hardware items are identified in the text by bold text (e.g., Power switch).
6
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
1 Introduction
1.2 Important user information
1.2
Important user information
Read this before operating
Fraction collector F9-C and
Fraction collector F9-R
All users must read the entire Operating Instructions before installing, operating or
maintaining Fraction collector F9-C and Fraction collector F9-R.
Always keep the Operating Instructions at hand when operating Fraction collector F9-C
and Fraction collector F9-R.
Do not operate Fraction collector F9-C and Fraction collector F9-R in any other way than
described in the user documentation. If you do, you may be exposed to hazards that
can lead to personal injury and you may cause damage to the equipment.
Intended use
Fraction collector F9-C and Fraction collector F9-R are automated fraction collectors
intended for the collection of fractions from ÄKTA™ pure purification runs. ÄKTA pure
and included modules are intended for research use only, and shall not be used in clinical
procedures, or for diagnostic purposes.
Prerequisites
In order to operate Fraction collector F9-C and Fraction collector F9-R safely and according to the intended purpose the following prerequisites must be met:
•
You should understand the concepts of liquid chromatography.
•
You should be familiar with the ÄKTA pure instrument and system and have read
the ÄKTA pure Operating Instructions.
•
You must read and understand the Safety chapter of these Operating Instructions.
•
The system must be installed according to the instructions in Chapter 4 Installation,
on page 44.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
7
1 Introduction
1.2 Important user information
Safety notices
This user documentation contains WARNINGS, CAUTIONS and NOTICES concerning the
safe use of the product. See definitions below.
Warnings
WARNING
WARNING indicates a hazardous situation which, if not avoided,
could result in death or serious injury. It is important not to proceed
until all stated conditions are met and clearly understood.
Cautions
CAUTION
CAUTION indicates a hazardous situation which, if not avoided,
could result in minor or moderate injury. It is important not to proceed until all stated conditions are met and clearly understood.
Notices
NOTICE
NOTICE indicates instructions that must be followed to avoid
damage to the product or other equipment.
Notes and tips
8
Note:
A note is used to indicate information that is important for trouble-free and
optimal use of the product.
Tip:
A tip contains useful information that can improve or optimize your procedures.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
1 Introduction
1.3 Regulatory information
1.3
Regulatory information
Introduction
This section lists the directives and standards that are fulfilled by Fraction collector F9-C
and Fraction collector F9-R.
Manufacturing information
The table below summarizes the required manufacturing information. For further information, see the EC Declaration of Conformity document.
Requirement
Content
Name and address of manufacturer
GE Healthcare Bio-Sciences AB,
Björkgatan 30, SE 751 84 Uppsala,
Sweden
Place and date of declaration
See EC Declaration of Conformity
Identity of person authorized to sign DoC
See EC Declaration of Conformity
CE Conformity
This product complies with the European directives listed in the table, by fulfilling the
corresponding harmonized standards.
The Declaration of Conformity is available on request.
Directive
Title
2006/42/EC
Machinery Directive (MD)
2004/108/EC
Electromagnetic Compatibility (EMC) Directive
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
9
1 Introduction
1.3 Regulatory information
CE Marking
The CE marking and the corresponding Declaration of conformity is valid for the fraction
collector when it is:
•
used as a stand-alone unit, or
•
connected to other CE marked instruments, or
•
connected to other products recommended or described in the user documentation,
and
•
used in the same state as it was delivered from GE Healthcare, except for alterations
described in the user documentation.
International standards
This product fulfills the requirements of the following standards:
Standard
Description
Notes
EN ISO 12100
Safety of machinery. General principles for design. Risk assessment and
risk reduction.
EN ISO standard is
harmonized with
EU directive
2006/42/EC
EN 61010-1, IEC 610101, UL 61010-1,
CAN/CSA C22.2 No.
61010-1
Safety requirements for electrical
equipment for measurement, control, and laboratory use.
No notes
EN 61326-1, IEC 613261
Electrical equipment for measurement, control and laboratory use EMC requirements
EN standard is harmonized with EU
directive
2004/108/EC
(Emission according to
CISPR 11, Group 1,
class A)
FCC 47 CFR Part 15
10
Federal Communications Commission (FCC) title 47 of the Code of
Federal Regulations (CFR), Part 15,
Radio Frequency Devices
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
1 Introduction
1.3 Regulatory information
FCC statement
Fraction collector F9-C and Fraction collector F9-R comply with FCC 47 CFR Part 15b.
Note:
This equipment has been tested and found to comply with the limits for a Class
A digital device, pursuant to part 15 of the FCC Rules. These limits are designed
to provide reasonable protection against harmful interference when the
equipment is operated in a commercial environment. This equipment generates,
uses and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to
radio communications. Operation of this equipment in a residential area is
likely to cause harmful interference in which case the user will be required to
correct the interference at his own expense.
Regulatory compliance of
connected equipment
Any equipment connected to Fraction collector F9-C and Fraction collector F9-R should
meet the safety requirements of EN 61010-1/IEC 61010-1, or other relevant harmonized
standards. Within EU, connected equipment must be CE marked.
Environmental Conformity
Directive
Title
2011/65/EU
Restriction of Hazardous Substances (RoHS) Directive
2002/96/EC
Waste Electrical and Electronic Equipment (WEEE) Directive
Regulation (EC) No
1907/2006
Registration, Evaluation, Authorization and restriction
of CHemicals (REACH)
ACPEIP
Administration on the Control of Pollution Caused by
Electronic Information Products, China Restriction of
Hazardous Substances (RoHS)
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
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2 Safety instructions
2
Safety instructions
About this chapter
This chapter describes safety precautions and emergency shutdown procedures for
Fraction collector F9-C and Fraction collector F9-R. The labels on the fraction collectors
and information regarding recycling are also described.
In this chapter
This chapter contains the following sections:
Section
12
See page
2.1 Safety precautions
13
2.2 Labels
20
2.3 Emergency procedures
23
2.4 Recycling information
25
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
2 Safety instructions
2.1 Safety precautions
2.1
Safety precautions
About this section
The safety precautions in this section are grouped in the following categories:
•
General precautions, on page 13
•
Flammable liquids, on page 14
•
Personal protection, on page 15
•
Installing and moving the fraction collector, on page 16
•
Operation, on page 16
•
Maintenance, on page 17
Unless stated otherwise, the precautions apply to both Fraction collector F9-C and
Fraction collector F9-R.
General precautions
WARNING
Do not operate Fraction collector F9-C and Fraction collector F9-R
in any other way than described in the Operating Instructions.
WARNING
Operation and user maintenance of Fraction collector F9-C and
Fraction collector F9-R should be performed by properly trained
personnel only.
WARNING
Do not use any accessories not supplied or recommended by GE
Healthcare.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
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2 Safety instructions
2.1 Safety precautions
WARNING
Do not use Fraction collector F9-C and Fraction collector F9-R if it
is not working properly, nor if it has suffered any damage, for example:
•
damage to the power cord or its plug
•
damage caused by dropping the equipment
•
damage caused by splashing liquid onto it
Flammable liquids
CAUTION
Explosion hazard during fractionation of flammable liquids. Do
not use a covered fraction collector (Fraction collector F9-C) to
fractionate flammable liquids. When using an open fraction collector (Fraction collector F9-R), do not place the fraction collector in
a closed compartment and make sure that the room ventilation
meets the local requirements.
Fraction collector F9-C
CAUTION
Fire Hazard. Do not fractionate flammable liquids using Fraction
collector F9-C. When running RPC methods, or other procedures
using organic solvents, collect fractions through the outlet valve
or Fraction collector F9-R.
CAUTION
Explosion hazard if flammable liquid leaks during cleaning of
the flow path. When cleaning the flow path of Fraction collector
F9-C with a flammable liquid like ethanol, carefully inspect the flow
path, including the waste tubing, to make sure there will be no
leakage.
14
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
2 Safety instructions
2.1 Safety precautions
Fraction collector F9-R
CAUTION
Fractionation with flammable liquids using Fraction collector
F9-R. Before you start the run, make sure that the tubes in the
fraction collector are of correct size according to fraction size settings.
CAUTION
Moving tubes with flammable liquids. Move tubes filled with
flammable liquids carefully, to prevent spillages that may come in
contact with an ignition source.
Personal protection
CAUTION
Always use appropriate personal protective equipment during operation and maintenance of Fraction collector F9-C and Fraction
collector F9-R.
CAUTION
Hazardous substances. When using hazardous chemical and biological agents, take all suitable protective measures, such as
wearing protective glasses and gloves resistant to the substances
used. Follow local and/or national regulations for safe operation,
maintenance and decommissioning of the equipment.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
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2 Safety instructions
2.1 Safety precautions
Installing and moving the
fraction collector
WARNING
UniNet cable. Only use UniNet cables delivered or approved by GE
Healthcare.
CAUTION
Heavy object. Use proper lifting equipment, or use two or more
persons when moving Fraction collector F9-C. All lifting and moving
must be performed in accordance with local regulations.
NOTICE
Vents on Fraction collector F9-C. To ensure adequate ventilation,
keep papers and other objects away from the vents of the fraction
collector.
Operation
CAUTION
Risk of breaking test vials. Do not use excessive force to press
vials with incorrect dimensions into the fraction collector. Glass
vials may break and cause injuries.
CAUTION
Avoid spillage and overflow. Make sure that the equipment is
prepared according to the settings in the method to be run.
16
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
2 Safety instructions
2.1 Safety precautions
Fraction collector F9-C
WARNING
Moving parts. Do not open the door of Fraction collector F9-C
while the fraction collector is active. If you need to access the
fraction collector, press Pause on the chromatography instrument,
and make sure that no parts are moving before opening the door.
CAUTION
Maximum weight on the top of Fraction collector F9-C. Do not
place more than 30 kg on top of Fraction collector F9-C.
Fraction collector F9-R
CAUTION
Replacement of the fraction collector bowl in Fraction collector
F9-R. Do not replace the bowl during a run.
CAUTION
Replacing tubes. Do not replace the tubes in the fraction collector
during a run.
Maintenance
CAUTION
Electrical shock hazard. All repairs should be done by service
personnel authorized by GE Healthcare. Do not open any covers
or replace parts unless specifically stated in the user documentation.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
17
2 Safety instructions
2.1 Safety precautions
CAUTION
Disconnect power. Always switch off power to Fraction collector
F9-C and Fraction collector F9-R, before cleaning any of its components, unless stated otherwise in the user documentation. This is
done by switching off the chromatographic instrument that provides Fraction collector F9-C and Fraction collector F9-R with
power.
CAUTION
Hazardous chemicals and biological agents. Before maintenance,
service and decommissioning, wash Fraction collector F9-C and
Fraction collector F9-R with a neutral solution to make sure that
any hazardous solvents and biological agents have been flushed
out from the fraction collector.
CAUTION
Always use appropriate personal protective equipment when decommissioning the equipment.
CAUTION
Cleaning Fraction collector F9-C and Fraction collector F9-R
before decommissioning.
•
Wipe Fraction collector F9-C and Fraction collector F9-R with
a damp tissue using a cleaning agent so that no hazardous
solvents or biological agents remain on the surface. Do not
forget to clean the interior of Fraction collector F9-C.
•
Perform a system CIP using a neutral solution. Make sure that
any hazardous solvents or biological agents are flushed out
from the fraction collector.
NOTICE
Cleaning. Keep Fraction collector F9-C and Fraction collector F9R dry and clean. Wipe regularly with a soft damp tissue and, if
necessary, a mild cleaning agent. Let Fraction collector F9-C and
Fraction collector F9-R dry completely before use.
18
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
2 Safety instructions
2.1 Safety precautions
CAUTION
Explosion hazard if flammable liquid leaks during cleaning of
the flow path. When cleaning the flow path of Fraction collector
F9-C with a flammable liquid like ethanol, carefully inspect the flow
path, including the waste tubing, to make sure there will be no
leakage.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
19
2 Safety instructions
2.2 Labels
2.2
Labels
About this section
This section describes the safety labels that are attached to Fraction collector F9-C and
Fraction collector F9-R.
Labels on Fraction collector F9-C
The label below is located both on the back of the fraction collector and on the top wall
of its interior.
Fraction collector F9-C
safety label
It is not allowed to fractionate flammable liquids using Fraction collector F9-C due to
the risk of build-up of flammable and explosive gases. When running RPC methods, or
other procedures using organic solvents, collect fractions through the Outlet valve or
with Fraction collector F9-R.
The safety label below is located on the inside of the door of Fraction collector F9-C.
3^=>CUaPRcX^]PcT
U[P\\PQ[T[X`dXSb
20
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
2 Safety instructions
2.2 Labels
Labels on Fraction collector F9-R
The label is located on the back of the fraction collector.
Label information
The label contains information on the serial number, regulatory compliance symbols,
recycling symbols, and specifications.
The label information is explained in the table below.
Label text
Meaning
Code no
Fraction collector code number
Serial no
Fraction collector serial number
Mfg Year
Manufacturing year
Voltage
Supply voltage
Max Power
Max power consumption
Protection class
Protection class, ingress protection according to IEC
60529
Warning! Read the Operating Instruction before using
the module. Do not open any covers or replace parts
unless specifically stated in the Operating Instructions.
The equipment complies with the applicable requirements for Australia and New Zealand
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
21
2 Safety instructions
2.2 Labels
Label text
Meaning
The fraction collector complies with applicable European directives
This symbol indicates that electrical and electronic
equipment must not be disposed of as unsorted municipal waste and must be collected separately. Please
contact an authorized representative of the manufacturer for information concerning the decommissioning
of equipment.
This symbol indicates that the product might contain
hazardous materials in excess of the limits established
by the Chinese standard SJ/T11363-2006 Requirements
for Concentration Limits for Certain Hazardous Substances in Electronic Information Products.
22
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
2 Safety instructions
2.3 Emergency procedures
2.3
Emergency procedures
About this section
The ÄKTA instrument supplies the Fraction collector F9-C and Fraction collector F9-R
with power. This section describes how to perform an emergency shutdown of the
Fraction collector F9-C and Fraction collector F9-R by shutting down the ÄKTA instrument.
This section also describes the results in the event of power failure or network interruption.
Emergency shutdown
In an emergency situation, stop the run by either pausing the run or switching off the
instrument as described below:
If you want
to...
then...
pause the run
•
press the Pause button on the Instrument control panel:
Note:
The buttons on the Instrument control panel may be locked. This
is an option available in the System settings.
or
•
click the Pause icon in UNICORN™:
Result: All pumps in the instrument are stopped.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
23
2 Safety instructions
2.3 Emergency procedures
If you want
to...
then...
switch off the
instrument
•
press the Power switch to the 0 position, or
•
disconnect the power cord from the wall socket.
Result: The run is interrupted immediately.
Note:
The sample and data may be lost as a result of switching off the
power.
Power failure
In case of a power failure or if communication is lost, the run is interrupted immediately
and all moving parts in the fraction collector are stopped.
24
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
2 Safety instructions
2.4 Recycling information
2.4
Recycling information
About this section
This section describes the procedures for disposal and recycling of Fraction collector
F9-C and Fraction collector F9-R.
Decommissioning and disposal
of the equipment
When taking Fraction collector F9-C and Fraction collector F9-R out of service:
•
The equipment must be decontaminated.
•
The components must be separated and recycled according to national and local
environmental regulations
CAUTION
Always use appropriate personal protective equipment when
decommissioning the equipment.
Disposal of electrical
components
Waste comprising electrical and electronic equipment must not be disposed of as unsorted municipal waste and must be collected separately. Please contact an authorized
representative of the manufacturer for information concerning the decommissioning of
equipment.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
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3 Fraction collector overview
3
Fraction collector overview
About this chapter
This chapter gives overviews of Fraction collector F9-C and Fraction collector F9-R.
In this chapter
This chapter contains the following sections:
Section
26
See page
3.1 Fraction collector F9-C
27
3.2 Fraction collector F9-R
37
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
3 Fraction collector overview
3.1 Fraction collector F9-C
3.1
Fraction collector F9-C
About this section
This section shows an overview of Fraction collector F9-C.
Technical details are found in the ÄKTA pure System handbook.
In this section
This section contains the following subsections:
Section
See page
3.1.1 Function
28
3.1.2 Fraction collector F9-C illustrations
29
3.1.3 Cassettes, Cassette tray and racks
33
-
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
27
3 Fraction collector overview
3.1 Fraction collector F9-C
3.1.1 Function
3.1.1
Function
The fraction collector collects fractions from ÄKTA pure purification runs.
The fraction collector can be used for:
•
Fixed volume fractionation
•
Peak fractionation
•
Combined fixed volume fractionation and peak fractionation
Fraction collector F9-C has the following functions for reducing sample spill during
fractionation:
28
•
Drop Sync
•
Accumulator
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
3 Fraction collector overview
3.1 Fraction collector F9-C
3.1.2 Fraction collector F9-C illustrations
3.1.2
Fraction collector F9-C illustrations
Introduction
This section provides illustrations of Fraction collector F9-C. The main features and
components are indicated.
Front view
The illustration below shows the main parts of the exterior of Fraction collector F9-C.
1
2
3
4
Part
Description
1
Fractionation indicator
5
6
Symbol indicating that fractionation is ongoing. Do not open the door while
the indicator is lit.
2
Door
3
Window
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
29
3 Fraction collector overview
3.1 Fraction collector F9-C
3.1.2 Fraction collector F9-C illustrations
Part
Description
4
Door handle
5
Tubing connector for outlet valve tubing
6
Vents
Rear view
The illustration below shows the rear view of Fraction collector F9-C.
1
1
3
30
2
Part
Description
1
Vents
2
UniNet-9 D-type connector (for communication and power supply)
3
Waste tube
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
3 Fraction collector overview
3.1 Fraction collector F9-C
3.1.2 Fraction collector F9-C illustrations
Interior
The illustration below shows the main parts of the inside of Fraction collector F9-C.
3
4
5
6
7
8
9
2
10
11
1
12
14
13
Part
Description
1
Fractionation arm guide rail
2
Fractionation arm main rail
3
Lamp
4
Tubing guide
5
Tubing connection
6
Dispenser head
7
Fractionation arm
8
Tubing guide
9
Height exclusion bar
10
Waste funnel
11
Waste tube
12
Tray catch
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
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3 Fraction collector overview
3.1 Fraction collector F9-C
3.1.2 Fraction collector F9-C illustrations
Part
Description
13
Waste groove, in case of overflow
14
Tray guides
Dispenser head
The illustration below shows the Dispenser head of Fraction collector F9-C.
1
2
3
4
6
32
5
Part
Description
1
Dispenser head
2
Nozzle
3
Dispenser head cover
4
Accumulator (back part of Dispenser head)
5
Drop sync sensor
6
Type code reader
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
3 Fraction collector overview
3.1 Fraction collector F9-C
3.1.3 Cassettes, Cassette tray and racks
3.1.3
Cassettes, Cassette tray and racks
Introduction
Fractions can be collected in deep well plates and in tubes of different sizes. A number
of cassettes, trays and racks for different tubes and deep well plates are available. The
cassettes are placed on a tray with six cassette positions.
The cassette type codes, tray type code and rack type codes are scanned by the type
code reader to determine the type of cassettes, trays and racks used. For more information on requirements that need to be fulfilled by the tubes and deep well plates to be
used in Fraction collector F9-C, see the ÄKTA pure System handbook .
Available cassettes, trays and
racks
The following Cassettes and racks are available:
•
Cassette 3 ml tubes (for 40 tubes)
•
Cassette 8 ml tubes (for 24 tubes)
•
Cassette 15 ml tubes (for 15 tubes)
•
Cassette 50 ml tubes (for 6 tubes)
•
Cassette for deep well plate (24, 48, 96 wells)
•
Cassette tray (for six cassettes)
•
Rack for 50 ml tubes (for 55 tubes)
•
Rack for 250 ml bottles (for 18 bottles)
For information on dimension requirements for tubes and deep well plates to be used
in the fraction collector, see Fraction collector tubes, on page 35 and Deep well plates,
on page 35 respectively.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
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3 Fraction collector overview
3.1 Fraction collector F9-C
3.1.3 Cassettes, Cassette tray and racks
Illustration of Cassettes on the
Cassette tray
The illustration below shows Cassettes placed on the Cassette tray.
Part
Description
1
Cassette tray
2
Cassette position number
3
Cassette
4
Cassette type code
5
Tubes or deep well plates placed in a Cassette
Illustrations of Fraction collector
F9-C tray and racks
The illustrations below show the Cassette tray, the Rack for 50 ml tubes and the Rack
for 250 ml bottles.
The fronts of the tray and the racks are marked with the GE-logotype.
In the Cassette tray, the cassette positions are marked 1 to 6.
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3 Fraction collector overview
3.1 Fraction collector F9-C
3.1.3 Cassettes, Cassette tray and racks
Cassette tray
Rack for 50 ml tubes
Rack for 250 ml bottles
Note:
The tray and racks are inserted into the fraction collector with the GE-logotype
facing outwards.
Note:
Do not use the Cassette tray when a rack for tubes or bottles is placed in the
fraction collector.
Fraction collector tubes
The tubes used in Fraction collector F9-C must fulfill the requirements listed in the table
below. Examples of manufacturers are also listed in the table.
Tube size
[ml]
Diameter [mm]
Height [mm]
Min.
Max.
Min.
Max.
Max.
volume
[ml]
Examples of
manufacturers
3
10.5
11.5
50
56
3
NUNC
8
12
13.3
96
102
8
BD Biosciences,
VWR
15
16
17
114
120
15
BD Biosciences
50
28
30
110
116
50
BD Biosciences
Deep well plates
Requirements
The deep well plates used in Fraction collector F9-C must fulfill the requirements listed
in the table below.
Property
Specification
No. of wells
24, 48, or 96
Shape of wells
Square, not cylindrical
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3 Fraction collector overview
3.1 Fraction collector F9-C
3.1.3 Cassettes, Cassette tray and racks
Property
Specification
Well volume
10, 5, or 2 ml
Approved deep well plates
The plates listed in the table below are tested and approved by GE Healthcare to be used
with Fraction collector F9-C.
Plate type
Manufacturer
Part no.
96 deep well plate
Eppendorf™
951033405/ 0030 501.306
BD Biosciences
353966
Greiner Bio-One
780270
Porvair Sciences
219009
Seahorse Bioscience™
S30009
Whatman™
7701-5200
Seahorse Bioscience
S30004
Whatman
7701-5500
Seahorse Bioscience
S30024
Whatman
7701-5102
48 deep well plate
24 deep well plate
36
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
3 Fraction collector overview
3.2 Fraction collector F9-R
3.2
Fraction collector F9-R
About this section
This section shows an overview of Fraction collector F9-R.
Technical details are found in the ÄKTA pure System handbook.
In this section
This section contains the following subsections:
Section
See page
3.2.1 Function
38
3.2.2 Fraction collector F9-R illustrations
39
3.2.3 Tubes
42
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37
3 Fraction collector overview
3.2 Fraction collector F9-R
3.2.1 Function
3.2.1
Function
The fraction collector collects fractions from ÄKTA pure purification runs.
The fraction collector can be used for:
•
Fixed volume fractionation
•
Peak fractionation
•
Combined fixed volume fractionation and peak fractionation
Fraction collector F9-R has the following function for reducing sample spill during fractionation:
•
38
Drop Sync
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3 Fraction collector overview
3.2 Fraction collector F9-R
3.2.2 Fraction collector F9-R illustrations
3.2.2
Fraction collector F9-R illustrations
Introduction
This subsection provides illustrations of Fraction collector F9-R. The main features and
components are indicated.
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3 Fraction collector overview
3.2 Fraction collector F9-R
3.2.2 Fraction collector F9-R illustrations
Front view
The illustration below shows the main parts of the Fraction collector.
3
4
2
5
6
1
7
8
40
Part
Function
1
Lock knob
2
Stationary part of delivery arm
3
Delivery arm
4
Tubing connector
5
Tube sensor
6
Collection tubes
7
Tube rack
8
Base unit
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3 Fraction collector overview
3.2 Fraction collector F9-R
3.2.2 Fraction collector F9-R illustrations
Connector panel
The illustration below shows the main parts of the connector panel on the fraction collector.
1
22
Part
Function
1
Node ID switch
2
UniNet-9 F-type connector (for communication and power supply)
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3 Fraction collector overview
3.2 Fraction collector F9-R
3.2.3 Tubes
3.2.3
Tubes
Introduction
For Fraction collector F9-R the fractions are collected in tubes of different sizes. For more
information on requirements that need to be fulfilled by the tubes to be used in Fraction
collector F9-R, see the ÄKTA pure System handbook.
Available tubes
Tubes with the following diameter can be used with Fraction collector F9-R:
•
12 mm
•
18 mm
•
30 mm
The tubes can have a tube length between 50-180 mm.
42
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3 Fraction collector overview
3.2 Fraction collector F9-R
3.2.3 Tubes
Illustration of the Fraction
collector F9-R Tube rack
Each tube rack is made up of a combination of a Bowl, Tube support, Tube guide and
Tube holder. For more information on the assembly of the tube rack, see Assemble the
Tube rack, on page 74. For information on which Tube rack to use, see the ÄKTA pure
System handbook.
Part
Function
1
Single cutout
2
L-shaped cutout
3
Bowl
4
Tube support
5
Tube guide
6
Tube holder
Note:
Note that the tube guide has both single and L-shaped cutouts, while the tube
holder only has single cutouts.
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4 Installation
4
Installation
About this chapter
This chapter provides required information to enable users and service personnel to install,
move and transport Fraction collector F9-C and Fraction collector F9-R.
In this chapter
Section
44
See page
4.1 Room and site requirements
45
4.2 Connect to ÄKTA pure
51
4.3 Prepare waste tubing for Fraction collector F9-C
60
4.4 Set the delay volume
62
4.5 Performance test
64
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4 Installation
4.1 Room and site requirements
4.1
Room and site requirements
About this section
This section describes the site planning and the preparations necessary for the installation
of Fraction collector F9-C and Fraction collector F9-R. The purpose is to provide planners
and technical staff with the data needed to prepare the laboratory for the installation.
In this section
Section
See page
4.1.1 Delivery and storage
46
4.1.2 Placement and space requirements
47
4.1.3 Site requirements
50
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45
4 Installation
4.1 Room and site requirements
4.1.1 Delivery and storage
4.1.1
Delivery and storage
About this section
This section describes the requirements for receiving the delivery box and storing Fraction
collector F9-C and Fraction collector F9-R before installation.
When you receive the delivery
•
Record on the receiving documents if there is any apparent damage on the delivery
box. Inform your GE Healthcare representative of such damage.
•
Move the delivery box to a protected location indoors.
Storage requirements
The delivery box should be stored in a protected place indoors. The following storage
requirements must be fulfilled for the unopened box:
46
Parameter
Allowed range
Ambient temperature, storage
-25°C to +60°C
Relative humidity
up to 90% atmospheric humidity at 40°C for 48
hrs
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
4 Installation
4.1 Room and site requirements
4.1.2 Placement and space requirements
4.1.2
Placement and space requirements
About this section
This section describes different options of where to place Fraction collector F9-C and
Fraction collector F9-R and the space required.
Location
Place the fraction collector on a clean, flat and stable area that is able to support the
weight of the fraction collector.
The following locations are recommended for the Fraction collector F9-C and the Fraction
collector F9-R:
•
on the bench to the left of the ÄKTA pure instrument
•
on a shelf below the bench
Note:
Longer tubing length increases back pressure and band broadening in the
chromatographic process. Place the fraction collector so that the total tubing
length is minimized.
Space requirements
The illustration below shows the space recommended for the system including Fraction
collector F9-C.
63 cm
60 cm
39 cm
54 cm
Min 10 cm
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
80 cm
47
4 Installation
4.1 Room and site requirements
4.1.2 Placement and space requirements
The illustration below shows the space recommended for the system including Fraction
collector F9-R.
63 cm
60 cm
32 cm
Note:
54 cm
Min 10 cm
80 cm
Fraction collector F9-R requires 10 cm free space above the fractionation arm
for handling of tubes.
Fraction collector weight
48
Item
Weight
Fraction collector F9-C
21 kg
Fraction collector F9-R
5 kg
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
4 Installation
4.1 Room and site requirements
4.1.2 Placement and space requirements
Fraction collector dimensions
The outer dimensions of Fraction collector F9-C are shown in the illustration below.
320 mm
585 mm
390 mm
The outer dimensions of Fraction collector F9-R are shown in the illustration below.
250 mm
400 mm
320 mm
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4 Installation
4.1 Room and site requirements
4.1.3 Site requirements
4.1.3
Site requirements
About this section
This section describes the site requirements for installation of Fraction collector F9-C
and Fraction collector F9-R.
Operating conditions
The following requirements must be fulfilled:
•
The instrument is intended for indoor use only.
•
The room must have exhaust ventilation.
•
The instrument should not be exposed to direct sunlight.
•
Dust in the atmosphere should be kept to a minimum.
Allowed operating conditions are specified in the table below.
Parameter
Allowed range
Ambient temperature, operating
4°C to 35°C
Ambient temperature, storage
-25°C to +60°C
Relative humidity, operating
20% to 95%, non condensing
Altitude
Maximum 2000 m
Pollution degree
2
Note:
50
Do not use buffers with a freezing point close to or below the temperature in
the room or in the cold room cabinet.
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4 Installation
4.2 Connect to ÄKTA pure
4.2
Connect to ÄKTA pure
About this section
This section contains information on how to set up tubing and a connection for power
and communication between Fraction collector F9-C or Fraction collector F9-R and the
ÄKTA pure instrument.
It is possible to install up to two fraction collectors in the following combinations when
using ÄKTA pure:
•
one Fraction collector F9-C and one Fraction collector F9-R, or
•
two Fraction collector F9-R.
If two fraction collectors are to be used, the following applies to the second one:
•
it must be a Fraction collector F9-R,
•
it has to be configured as Fraction collector F9-R, 2nd,
•
the node ID has to be changed, as described in Check/change Node ID, on page 56.
In this section
Section
See page
4.2.1 Power and communication
52
4.2.2 Connect tubing
58
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51
4 Installation
4.2 Connect to ÄKTA pure
4.2.1 Power and communication
4.2.1
Power and communication
Introduction
This subsection describes how to set up the power and communication connection between Fraction collector F9-C or Fraction collector F9-R and the ÄKTA pure instrument.
Connect fraction collector
The following cables are used to connect the fraction collectors to the ÄKTA pure instrument. Use the specified cable and follow the instructions below.
Fraction collector
Cable
Fraction collector F9-C
UniNet-9 cable, D-type
Fraction collector F9-R
UniNet-9 cable, F-type
WARNING
UniNet cable. Only use UniNet cables delivered or approved by GE
Healthcare.
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4 Installation
4.2 Connect to ÄKTA pure
4.2.1 Power and communication
Step
Action
1
Switch off the power to the ÄKTA pure instrument.
2
Remove the jumper from the UniNet-9 port to be used on the rear of the
ÄKTA pure instrument (port 7 or 8 for Fraction collector F9-C, port 1 to 6 for
Fraction collector F9-R).
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53
4 Installation
4.2 Connect to ÄKTA pure
4.2.1 Power and communication
Step
Action
3
Connect the UniNet-9 cable between the UniNet-9 ports on the back of the
fraction collector and on the back of the ÄKTA pure instrument.
NOTICE
Do not use the Test port on the ÄKTA pure instrument.
2
Fraction collector F9-C
4
Fraction collector F9-R
Make sure that all unused UniNet-9 ports on the ÄKTA pure instrument are
plugged with jumpers.
Software configuration
When the fraction collector has been installed, the System properties for the system
has to be updated in UNICORN. The system will restart automatically when the configuration has been changed and the system can be reconnected.
From hereon, UNICORN refers to UNICORN 6.3 or other compatible versions of the software. The examples given in these Operating Instructions refer to UNICORN 6.3.
Follow the instructions below to update the system in UNICORN.
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4 Installation
4.2 Connect to ÄKTA pure
4.2.1 Power and communication
Step
Action
1
•
In the Administration module, choose Tools:System Properties or click
the System Properties icon to open the dialog.
Result: The System Properties dialog is displayed.
•
Select a system in the System Properties dialog.
Note:
Only active systems can be edited.
•
Click the Edit button.
Result: The Edit dialog is displayed.
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55
4 Installation
4.2 Connect to ÄKTA pure
4.2.1 Power and communication
Step
Action
2
In the Edit dialog:
•
Select Fraction collectors from the Component types list.
Result: All available fraction collectors are shown in the Component selection list.
Note:
Instrument modules are referred to as Components in UNICORN.
Note:
An outlet valve has to be selected in order to select a fraction collector.
•
Select the Fraction collector check box.
•
Choose what kind of fraction collector to add from the drop down list
box.
•
Note:
Fraction collector 2 is only available in the Component selection list if
Fraction collector is already selected.
3
Click the OK button to apply the changes.
Check/change Node ID
The Node ID for Fraction collector F9-C cannot be changed.
The Node ID for Fraction collector F9-R needs to be changed if
•
56
the fraction collector will be used as secondary fraction collector, fraction collector,
2nd.
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4 Installation
4.2 Connect to ÄKTA pure
4.2.1 Power and communication
•
the fraction collector is to be used as primary fraction collector after it has been used
as secondary.
The Node ID for Fraction collector F9-R is set by positioning the arrow of the rotating
switch at the back of the Fraction collector. Use a screwdriver to set the arrow of the
switch to the desired number.
Fraction collector
Order
Node ID
Fraction collector F9-C
1st
0
Fraction collector F9-R
1st
0
2nd
1
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57
4 Installation
4.2 Connect to ÄKTA pure
4.2.2 Connect tubing
4.2.2
Connect tubing
Introduction
This subsection contains information on how to connect tubing from ÄKTA pure to
Fraction collector F9-C and Fraction collector F9-R.
Fraction collector F9-C
Fraction collector F9-C is delivered with all internal tubing in place. The tubing between
the fraction collector and ÄKTA pure need to be installed.
Follow the instructions in the table below to connect the tubing from the ÄKTA pure instrument to the fraction collector.
58
Step
Action
1
Connect the tubing Frac to the Frac port on the outlet valve on the ÄKTA
pure instrument.
2
Connect the other end of the Frac tubing to the inlet port on the fraction
collector.
3
Adjust the delay volume setting in UNICORN, see Section 4.4 Set the delay
volume, on page 62 for more details.
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4 Installation
4.2 Connect to ÄKTA pure
4.2.2 Connect tubing
Fraction collector F9-R
Follow the instructions in the table below to connect the tubing from the fraction collector
to the ÄKTA pure instrument.
Step
Action
1
Lift out the Tubing holder (4) from the Delivery arm (1).
3
5
6
4
2
Loosen the nut of the Tubing holder. Do not remove the Tubing holder nut
(5) from the Tubing holder.
3
Insert the tubing through the Tubing holder.
4
Place the Tubing holder with the tubing over the Tube adjustment cavity (2)
of the Delivery arm. Push the tubing down against the bottom of the Tube
adjustment cavity, and then fingertighten the Tubing holder nut. This ensures
the correct length of the exposed tubing end (6).
5
Re-install the tubing holder in the Delivery arm.
6
For Fraction collector F9-R: Connect the tubing from the fraction collector
to the port Frac on the outlet valve.
For Fraction collector F9-R, 2nd: Connect the tubing from the second fraction
collector to:
7
•
port Out 10 on Outlet valve V9-O
•
port Out 1 on Outlet valve V9-Os
Adjust the delay volume setting in UNICORN to the volume of the tubing, see
Section 4.4 Set the delay volume, on page 62 for more details.
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4 Installation
4.3 Prepare waste tubing for Fraction collector F9-C
4.3
Prepare waste tubing for Fraction collector F9-C
The waste outlet is located on the rear of the fraction collector. See instructions below
for how to prepare the waste tubing.
Instructions
Follow the instructions below to prepare the waste tubing.
Step
Action
1
Insert the waste tubing in a vessel.
NOTICE
The maximum level of the vessel for the waste tubing
must be lower than the fraction collector.
60
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4 Installation
4.3 Prepare waste tubing for Fraction collector F9-C
Step
Action
2
Cut the waste tubing to appropriate length. It is important that the tubing
is not bent and will not be submerged in liquid during the run.
Note:
If the tubing is too short, replace it with new tubing. Do not lengthen the tubing
as this might cause obstruction of the tubing.
3
Securely fasten the tubing to the waste vessel.
CAUTION
Make sure that the waste vessel will hold all the produced volume
of the run.
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4 Installation
4.4 Set the delay volume
4.4
Set the delay volume
Introduction
The delay volume settings are used to make sure that the fractions collected during
fractionation, using the outlet valve or the fraction collector, correspond to the fractions
indicated in the chromatogram.
Set the delay volume
Follow the instructions below to set the delay volume between the UV monitor and the
outlet valve and between the UV monitor and the fraction collector. Refer to the ÄKTA
pure System handbook for more information about delay volumes.
Step
1
Action
Select System:Settings in the System Control module.
Result: The System Settings dialog opens.
2
62
•
Select Tubing and Delay Volumes and select Delay volume: Monitor to
outlet valve.
•
Type in the volume in the Volume field and click OK.
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4 Installation
4.4 Set the delay volume
Step
Action
3
In the System Settings dialog:
•
Select Delay volume: Monitor to outlet valve or Monitor to frac according to the configuration you are using.
•
Type in the volume in the Volume field and click OK.
Note:
The system will use the delay volume appropriate to the configuration used
and ignore other settings (e.g. the value for Monitor to outlet valve will be
ignored if you are using a fraction collector). It is however recommended to
set all delay volumes so that the volumes remain correct if you change fractionation method.
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4 Installation
4.5 Performance test
4.5
Performance test
Before taking the Fraction collector F9-C and Fraction collector F9-R instrument into
use, run a performance test to check the function of the equipment. See the ÄKTA pure
System handbook for further instructions.
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5 Operation
5
Operation
About this chapter
This chapter describes how to prepare and assemble the fraction collector before a run.
In this chapter
Section
See page
5.1 Fraction collector F9-C
66
5.2 Fraction collector F9-R
73
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65
5 Operation
5.1 Fraction collector F9-C
5.1
Fraction collector F9-C
About this section
This section describes how to prepare and assemble Fraction collector F9-C before a
run.
The fraction collector is connected to ÄKTA pure and controlled by UNICORN. Control of
the fraction collector can be achieved automatically in a method run, or manually.
CAUTION
Fire Hazard. Do not fractionate flammable liquids using Fraction
collector F9-C. When running RPC methods, or other procedures
using organic solvents, collect fractions through the outlet valve
or Fraction collector F9-R.
Note:
The tray and racks can tilt slightly when not fully inserted into the fraction
collector and may harm the fractionation arm. The tilt is due to the height difference between the door and the floor of the fraction collector. The tendency
to tilt is affected by the placement and weight of the cassettes, tubes or bottles
Prepare the fraction collector
Before starting to prepare Fraction collector F9-C, check the fractionation settings in the
method to be run. Perform the steps described below according to the settings in the
method.
•
Insert the Cassette tray or a rack for tubes or bottles.
•
Change the System Settings in UNICORN to set the fractionation mode and other
settings for fraction collection.
How to insert a tray or a rack is shown below.
For information on how to change the System Settings before a run, see the ÄKTA pure
System handbook.
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5 Operation
5.1 Fraction collector F9-C
Prepare and insert the Cassette
tray
Follow the instructions below to add cassettes to the Cassette tray and insert the tray
into the fraction collector.
Step
Action
1
If you are to use cassettes with the QuickRelease function, open the cassettes.
For more information on the QuickRelease function see the ÄKTA pure System
handbook.
2
Place the tubes and deep well plates in the cassettes. Make sure that the
deep well plates are rotated so that the well marked A1 is positioned above
the A1 marking on the cassette.
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67
5 Operation
5.1 Fraction collector F9-C
68
Step
Action
3
Close the cassettes that have the QuickRelease function.
4
Place the cassettes on the Cassette tray. Make sure that the cassette type
code (see illustration below) faces the front of the tray marked with the GE
logo.
5
Open the door of the fraction collector using the handle.
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5 Operation
5.1 Fraction collector F9-C
Step
Action
6
Insert the tray into the fraction collector:
•
make sure that the front of the tray (marked with the GE logo) faces
outwards
•
position the tray and slide it into the fractionation collector until it
reaches the end
•
make sure that the Tray catch snaps into closed position, as shown below.
Note:
The tray can tilt slightly when not fully inserted into the fraction collector and
may harm the fractionation arm. The tilt is due to the height difference between
the door and the floor of the fraction collector. The tendency to tilt is affected
by the placement and weight of the cassettes.
Note:
A height exclusion bar ensures that the tubes and deep well plates are correctly positioned and cannot damage the dispenser head.
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5 Operation
5.1 Fraction collector F9-C
Step
Action
7
Close the door. Make sure that it closes properly.
Result: After the door has been closed, the fractionation arm scans the cassette type code of each cassette to identify the cassette types. If deep well
plates are used, the instrument also identifies the types of deep well plates.
Note:
If the rack is inserted with the front of the rack facing the wrong way it will
not be possible to close the door.
Prepare and insert a rack for
tubes or bottles
Follow the instructions below to insert a rack for tubes or bottles into the fraction collector.
70
Step
Action
1
Place the tubes or bottles in the rack.
2
Open the door of the fraction collector using the handle.
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5 Operation
5.1 Fraction collector F9-C
Step
Action
3
Insert the rack into the fraction collector:
•
make sure that the front of the rack (marked with the GE logo) faces
outwards
•
position the rack and slide it into the fractionation collector until it
reaches the end
•
make sure that the Tray catch snaps into closed position, as shown below.
Note:
The rack can tilt slightly when not fully inserted into the fraction collector and
may harm the fractionation arm. The tilt is due to the height difference between
the door and the floor of the fraction collector. The tendency to tilt is affected
by the placement and weight of the tubes or bottles.
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5 Operation
5.1 Fraction collector F9-C
Step
Action
Note:
A height exclusion bar ensures that the tubes and deep well plates are correctly positioned and cannot damage the Dispenser head.
Note:
Do not use the Cassette tray when the Rack for 50 ml tubes or the Rack for
250 ml bottles is placed in the fraction collector.
4
Close the door. Make sure that it closes properly.
Note:
If the rack is inserted with the front of the rack facing the wrong way it will
not be possible to close the door.
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Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
5 Operation
5.2 Fraction collector F9-R
5.2
Fraction collector F9-R
About this section
This section describes how to prepare and assemble Fraction collector F9-R before a
run.
The Fraction collector F9-R is connected to ÄKTA pure and controlled by UNICORN.
Control of the fraction collector can be achieved automatically in a method run, or
manually.
Prepare the fraction collector
Before starting to prepare Fraction collector F9-R, check the fractionation settings in the
method to be run. Perform the steps described below according to the settings in the
method.
•
Assemble the tube rack
•
Insert collection tubes
•
Adjust the Delivery arm
•
Change the System Settings in UNICORN to set the fractionation mode and other
settings for fraction collection.
It is shown how to assemble the tube rack, insert collection tubes and adjust the delivery
arm below. For more information on how to change the System Settings before a run,
see the ÄKTA pure System handbook.
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73
5 Operation
5.2 Fraction collector F9-R
Assemble the Tube rack
Follow the instructions below to assemble the Tube rack. See the ÄKTA pure System
handbook for information about which Tube rack parts to use.
Step
Action
1
Insert the Tube support (4), if required, into the bowl (3). The circular marks
on the Tube support should face down.
Note:
When assembling a Tube rack, Single cutouts (1) and L-shaped cutouts (2),
are used for various inserts depending on the length of the collection tubes.
See ÄKTA pure System handbook for detailed information.
2
74
Insert the Tube guide (5) with the tube position numbers upwards. The Tube
guide should rest about 1 cm above the Tube support.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
5 Operation
5.2 Fraction collector F9-R
Step
Action
3
Insert the Tube holder (6) with the tube position numbers upwards:
•
Check that tube position 1 (7) is directly above tube position 1 (7) of the
Tube guide.
7
•
Push the flexible bowl out at each rib and snap the Tube holder under
the top lip of the rib (8).
8
Note:
Do not force the tube holder into place as this may damage the lip.
•
4
Check that the surface of the Tube holder is level.
Gently move the Delivery arm (10) out to the outer stop.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
75
5 Operation
5.2 Fraction collector F9-R
Step
Action
5
Place the Tube rack (9) over the Central spindle (11) and pull the spring
loaded Drive sleeve (12) out so the Tube rack comes to rest.
9
10
12
11
Insert collection tubes
Insert sufficient collection tubes in to the Tube rack, starting at position 1, pushing each
one down as far as they will go. All the tubes must be of the same length and diameter
and there should be no empty positions in the sequence.
76
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
5 Operation
5.2 Fraction collector F9-R
Adjust the Delivery arm
Follow the instructions in the table below to adjust the Delivery arm.
Step
Action
1
Gently lift and then lower the Delivery arm (1), and allow it to move in so the
Tube sensor (2) touches the collection tubes of the outer track.
1
2
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
77
5 Operation
5.2 Fraction collector F9-R
Step
Action
2
Adjust the height of the Delivery arm as follows:
•
Loosen the lock knob (3)
3
•
Adjust the height so that the horizontal mark (5) on the Tube sensor (6)
is at the same level as the top of the flat collection tubes and approximately 2 mm over the top of the flanged collection tubes (4).
4
5
6
•
78
Lock the Delivery arm at this height with the lock knob.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
5 Operation
5.2 Fraction collector F9-R
Step
Action
3
Check that the Tube sensor (9) is in the correct position for the used tubes
(10). The eluent tubing (7) should be above the center of the collection tube.
Use the Sensor control (8) to position the Tube holder.
7
8
9
10
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
79
6 Maintenance
6
Maintenance
About this chapter
This chapter describes maintenance and provides instructions for how to clean Fraction
collector F9-C and Fraction collector F9-R. For further information, see the ÄKTA pure
System handbook.
CAUTION
Explosion hazard if flammable liquid leaks during cleaning of
the flow path. When cleaning the flow path of Fraction collector
F9-C with a flammable liquid like ethanol, carefully inspect the flow
path, including the waste tubing, to make sure there will be no
leakage.
In this chapter
Section
80
See page
6.1 Fraction collector F9-C
81
6.2 Fraction collector F9-R
83
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
6 Maintenance
6.1 Fraction collector F9-C
6.1
Fraction collector F9-C
Maintenance interval
Clean the fraction collector when required, for example if liquid has been spilled in the
fraction collector chamber.
The internal tubing of the fraction collector may need to be replaced for maintenance
or for process purposes. Information on how and when to replace the internal tubing
can be found in the ÄKTA pure System handbook.
Required material
The following material is required:
•
Wash bottle
•
Water or 20% ethanol
•
Cloth
Instruction
Follow the instruction below to clean the interior of the Fraction collector. The locations
of the components of the fraction collector are shown in Section 3.1.2 Fraction collector
F9-C illustrations, on page 29.
Step
Action
1
In System Control, select Manual:Execute Manual Instructions:Fraction
collection:Frac cleaning position . Click Execute.
Result: The Dispenser head moves to cleaning position, and the Instrument
display states System pause.
2
Open the Frac door and remove the rack
3
Wash the Cassette tray or rack and the Cassettes (if applicable), with water
and a mild cleaning agent.
4
Lift off the Waste groove and wash it with water and a mild cleaning agent.
Refit the Waste groove.
5
Wipe off the interior of the fraction collector using a damp cloth. Wipe off
stains using a mild cleaning agent or 20% ethanol.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
81
6 Maintenance
6.1 Fraction collector F9-C
Step
Action
6
Wipe off the Dispenser head and its diode windows (the Drop sync sensor
and the Type code reader) using a wash bottle with water or 20% ethanol
and a cloth.
7
Let the fraction collector dry completely before starting a run.
8
Close the door of the fraction collector.
Result: Automatic scanning is performed.
9
In the System Control module, press the End icon in the toolbar.
Result: The Dispenser head moves to home position.
82
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
6 Maintenance
6.2 Fraction collector F9-R
6.2
Fraction collector F9-R
Maintenance interval
Clean the Fraction collector when required, for example in case of liquid spill.
Required material
The following material are required:
•
Water or 20% ethanol
•
Cloth
Instruction
Follow the instructions below to clean the instrument externally.
Step
Action
1
Check that no run is in progress.
2
Switch off the instrument.
3
Wipe the surface with a damp cloth. Wipe off stains using a mild cleaning
agent or 20% ethanol. Wipe off any excess.
4
Let the Fraction collector F9-R dry completely before restart.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
83
7 Reference information
7
Reference information
See the ÄKTA pure Product information and the ÄKTA pure System handbook for information on the following:
•
Technical specifications
•
Chemical resistance
For information on operating conditions, see Section 4.1.3 Site requirements, on page 50.
Environmental ranges
Parameter
Data
Storage and transport temperature range
-25°C to +60°C
Chemical environment
See the ÄKTA pure System handbook.
Equipment noise level
Equipment
Acoustic noise
level
Fraction collector F9-C
< 60 dB A
Fraction collector F9-R
< 60 dB A
Heat output
Equipment
Heat output
Fraction collector F9-C
Typically 6 W
Maximum 18 W
Fraction collector F9-R
84
2W
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
A Declaration of Hazardous Substances (DoHS)
Appendix A Declaration of Hazardous
Substances (DoHS)
Introduction
The following product pollution control information is provided according to SJ/T113642006 Marking for Control of Pollution caused by Electronic Information Products.
㫈㗽SJ/T11364-2006ᇵ䟄⷟≰㋾ℶ❐㻰㩢㘶Ⓟ㪖幕尐㻑ᇶ䔈㙟∪Ⱁₚ㦘␂㻰㩢 㘶Ⓟ
㡈槱䤓≰㋾
Symbols used in pollution control
label
䟄⷟≰㋾ℶ❐㻰㩢㘶Ⓟ㪖㉦広㢝
Label
Meaning
This symbol indicates the product contains hazardous materials in excess of the limits established by the Chinese standard SJ/T11363-2006
Requirements for Concentration Limits for Certain Hazardous Substances in Electronic Information Products. The number in the symbol
is the Environment-friendly Use Period (EFUP), which indicates the period
during which the toxic or hazardous substances or elements contained
in electronic information products will not leak or mutate under normal
operating conditions so that the use of such electronic information
products will not result in any severe environmental pollution, any
bodily injury or damage to any assets. The unit of the period is “Year”.
In order to maintain the declared EFUP, the product shall be operated
normally according to the instructions and environmental conditions
as defined in the product manual, and periodic maintenance schedules
specified in Product Maintenance Procedures shall be followed strictly.
Consumables or certain parts may have their own label with an EFUP
value less than the product. Periodic replacement of those consumables
or parts to maintain the declared EFUP shall be done in accordance
with the Product Maintenance Procedures.
This product must not be disposed of as unsorted municipal waste,
and must be collected separately and handled properly after decommissioning.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
85
A Declaration of Hazardous Substances (DoHS)
Label
Meaning
年㪖㉦嫷㢝㦻ℶ❐⚺㦘怔扖SJ/T11363-2006ᇵ䟄⷟≰㋾ℶ❐₼㦘㹡
㦘⹂䓸德䤓棟 摞尐㻑ᇶ₼棟摞䤓㦘㹡㦘⹂䓸德ᇭ㪖㉦₼䤓㟿ⷦ⃉㦻
ℶ❐䤓䘾≬∎䞷㦮᧨嫷㢝㦻 ℶ❐⦷㷲デ∎䞷䤓㧰ↅₚ᧨㦘㹡㦘⹂䓸
德ₜ↩♠䞮⮥㽓㒥䴐♧᧨䞷㓆∎䞷㦻ℶ❐ ₜ↩⺈䘾⬒抯㒟₴摜㻰㩢
㒥⺈␅ⅉ愺ᇬ徱ℶ抯㒟₴摜㗮⹂䤓㦮棟ᇭ◤⇜⃉㄃ᇭ
⃉≬幐㓏䟂㢝䤓䘾≬∎䞷㦮棟᧨ㄣ㖘ℶ❐㓚␛₼㓏屓⸩䤓䘾⬒㧰ↅ
✛㡈㽤扪嫛㷲 デ∎䞷᧨ㄅ₴㫋挄⸗ℶ❐冃≽㓚␛₼屓⸩䤓㦮冃≽✛
≬␊尐㻑ᇭ
ℶ❐₼䤓䀗劦ↅ✛㩟K榅捷ↅ♾厌㦘␅◤䕻䤓䘾≬∎䞷㦮棟㪖㉦᧨
ㄅ₣␅䘾≬∎ 䞷㦮棟㦘♾厌㹣㠃₹ℶ❐㦻愺䤓䘾≬∎䞷㦮棟䩼ᇭㄣ
Ⓙ㦮㖘ℶ❐冃≽䲚ㄞ㦃㗱挲 K䀗劦ↅ✛榅捷ↅ᧨ⅴ≬幐㓏䟂㢝䤓㠃
₹ℶ❐䤓䘾≬∎䞷㦮棟ᇭ
㦻ℶ❐⦷∎䞷⺎✌兢㧮㢅ₜ♾⇫⃉㣽抩䞮㿊⨒⧍⮓䚕᧨ㄣ嬺◤䕻㟅
楕ⰴ⠓⮓䚕
List of hazardous substances and
their concentrations
ℶ❐₼㦘㹡㦘⹂䓸德㒥⏒侯䤓⚜䱿♙⚺摞
Indication for each major part if substance exceeds limit
Value
Meaning
O
Indicates that this toxic or hazardous substance contained in all of the
homogeneous materials for this part is below the limit requirement in
SJ/T11363-2006.
嫷䯉年㦘㹡㦘⹂䓸德⦷年捷ↅ㓏㦘⧖德㧟㠨₼䤓⚺摞⧖⦷SJ/T113632006 㪖⑕屓⸩䤓棟摞尐 㻑ⅴₚ
X
Indicates that this toxic or hazardous substance contained in at least
one of the homogeneous materials used for this part is above the limit
requirement in SJ/T11363-2006.
•
Data listed in the table represents best information available at the
time of publication
嫷䯉年㦘㹡㦘⹂䓸德咂⺠⦷年捷ↅ䤓㩟₏⧖德㧟㠨₼䤓⚺摞怔⒉
SJ/T11363-2006 㪖⑕屓⸩䤓
棟摞尐㻑
•
86
㷳嫷㓏⒦㟿㗽⃉♠を㢅㓏厌噆㈦䤓㦏∂≰㋾
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
A Declaration of Hazardous Substances (DoHS)
List of hazardous substances
Component
name
捷ↅ⚜䱿
1
Hazardous substance
㦘㹡㦘⹂䓸德㒥⏒侯
Pb
Hg
Cd
Cr6+
PBB
PBDE
杔
㻭
柘
⏼ↆ杻
⮩䅃勣啾
⮩䅃ℛ啾搩
29-0277-43 1
0
0
0
0
0
0
29-0113-621
0
0
0
0
0
0
The product has not been tested as per the Chinese standard SJ/T11363-2006 Requirements
for Concentration Limits for Certain Hazardous Substances in Electronic Information Products.
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
87
Index
Index
A
ÄKTA pure
Cassettes and racks, 33
preparation, 66, 73
C
Labels, 20
Fraction collector F9-C instrument label, 21
M
Cassettes, 33
CE
Conformity, 9
Marking, 10
Manufacturing information, 9
N
Notes and tips, 8
D
Delay volume
set, 62
Dimensions
instrument, 49
E
Emergency procedures
emergency shutdown, 23
F
Fractionation
assemble tube rack, 43
Fraction collector F9-C
Cassettes and racks, 33
cleaning, 81
connect tubing, 58
requirements on deep well
plates, 35
requirements on tubes, 35
Tubes, 42
Fraction collector F9-R
cleaning, 83
connect tubing, 59
Delivery arm, 77
Insert collection tubes, 76
Tube rack, 74
I
Installation
performance test, 64
Instrument overview
Fraction collector F9-R, 40
88
L
O
Operating conditions, 50
P
Prepare the system
ÄKTA pure, 66, 73
waste tubing, 60
Prerequisites, 7
Purpose of this document, 6
R
Racks, 33
Recycling
disposal, 25
Recycling procedures, 25
Reference information
recycling, 25
Regulatory information
international standards, 10
regulatory compliance of
connected equipment, 11
S
Safety notices, 8
Safety precautions, 13
emergency procedures, 23
labels, 20
Storage, 46
T
Tube rack
assembly, 43
Typographical conventions, 6
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
Index
U
W
User information, important, 7
Waste tubing
prepare, 60
Weight
instrument, 48
Fraction collector F9-C and Fraction collector F9-R Operating Instructions 29-0256-06 AA
89
For local office contact information, visit
www.gelifesciences.com/contact
GE, imagination at work and GE monogram are trademarks of General Electric
Company.
GE Healthcare Bio-Sciences AB
Björkgatan 30
751 84 Uppsala
Sweden
Seahorse Bioscience is a trademark of Seahorse Bioscience Corporation.
www.gelifesciences.com/AKTA
ÄKTA, UNICORN and Whatman are trademarks of GE Healthcare companies.
Eppendorf is a trademark of Eppendorf AG.
© 2012 General Electric Company – All rights reserved.
First published Nov. 2012
All goods and services are sold subject to the terms and conditions of sale of
the company within GE Healthcare which supplies them. A copy of these terms
and conditions is available on request. Contact your local GE Healthcare representative for the most current information.
UNICORN: Any use of this software is subject to GE Healthcare Standard Software
End-User License Agreement for Life Sciences Software Products. A copy of this
Standard Software End-User License Agreement is available on request.
GE Healthcare Europe GmbH
Munzinger Strasse 5, D-79111 Freiburg, Germany
GE Healthcare UK Limited
Amersham Place, Little Chalfont, Buckinghamshire, HP7 9NA, UK
GE Healthcare Bio-Sciences Corp.
800 Centennial Avenue, P.O. Box 1327, Piscataway, NJ 08855-1327, USA
GE Healthcare Japan Corporation
Sanken Bldg. 3-25-1, Hyakunincho Shinjuku-ku, Tokyo 169-0073, Japan
imagination at work
29-0256-06 AA 11/2012