Hellenic Accreditation System
Annex F1/H3 to the Certificate No. 142-3
SCOPE of ACCREDITATION
of the
A’ Chemical Division
of the
General Chemical State Laboratory
Tested materials/ products
Types of test/ Properties to be measured
Applied Standards/ Techniques to be
used
Biological tests
1. Soya: foodstuffs (beans,
flour), feedstuffs (flour)
Screening method for genetically
modified organisms (GMOs), by the
detection of CaMV 35S promoter.
2. Maize: foodstuffs (grains,
flour), feedstuffs (grains,
flour)
In house method (ΜΕΘ 0302 ΓΤΟ1)
based on the Official German Method
[LMBG § 35 of the German Federal
Foodstuffs Act, L 00.00.31 (1998)] and
by using the Eurofins GeneScan kit
(GMOScreen 35S/NOS), with PCR and
electrophoresis.
DNA extraction with the DNAExtractor
kit, Eurofins GeneScan.
Chemical testing
1. Soya sauce
1. Determination of Chloropropanols in
Foodstuffs
2. Fats and oils, Meat and
1. Determination of polycyclic aromatic
meat products, Cheese,
hydrocarbons in foodstuffs
Fishes, Milk powder,
Dried fruits, Spices, Infant
formulas, Food
supplements, Cocoa beans
and its products
Page 1 of 6
In house method: ΜΕΘ 03 02 CPGCMS
GC-MS, based on ΕΛΟΤ EN
14573:2005
In house method: ΜΕΘ 03 02 PAHHPLC
HPLC_UV-VIS-Fluorecence based on
ISO-WD 15753:2006
Annex F1/H3 to the Certificate ESYD No. 142-3
19.11.2015
Tested materials/ products
Types of test/ Properties to be measured
Applied Standards/ Techniques to be
used
1. Detection of allergenic peanut protein
In house method: ΜΕΘ 03 02 ΑΛΛ 01
based on the Biokits Peanut Assay Kit
(NEOGEN) and according to EN
15633-1:2009 Foodstuffs-Detection of
food allergens by immunological
methods Part1: General considerations
2. Determination of Cd, Pb
In house method 04 01 AASF1 based on
ELOT EN 14084:2003, ELOT EN
13604:2002 and AOAC 999.10:2000
with AAS with graphite furnaceZeeman
4. Coffee raisins and cereals
Determination of ochratoxin A.
Method 04 01 OTA: In–house method
using high performance liquid
chromatography (HPLC) after
immunoaffinity column cleanup.
5. Milk
Determination of aflatoxin M1.
Method 04 01 M1M: In–house method
using high performance liquid
chromatography (HPLC) after
immunoaffinity column cleanup.
6. Edible plant fats and oils
1. Determination of free fatty acids
3. Foodstuffs
2. Determination of peroxide value
3. Determination of fatty acid methyl
esters
4. Determination of sterols and
erythrodiol-uvaol
7. Olive oils and pomace oils
1. Spectrophotometric analysis in the
ultraviolet
ISO 660:2009
Method of Regulation EEC 2568/91,
Annex II / Titration, as in force
Method Code : 15 01 Λ1
Method of Regulation EEC 2568/91,
Annex III / Titration, as in force
Method Code : 15 01 Λ3
ISO 5508:1990.
Method of Regulation EEC 2568/91,
Annex X / Gas Chromatography, as in
force
Method Code : 15 01 Λ6
Method of Regulation EEC 2568/91,
Annex V and VI / TLC, Gas
Chromatography, as in force
Method Code : 15 01 Λ8
Method of Regulation EEC 2568/91,
Annex IX / Spectrophotometry, as in
force
Method Code : 15 01 Λ2
Method of Regulation EEC 2568/91,
2. Determination of triacylglyceroles with
Annex XVIII, HPLC, as in force
ECN42 (ΔECN42)
Method Code : 15 01 Λ7
3. Determination of waxes
Page 2 of 6
Method of Regulation EEC 2568/91,
Annex IV, column chromatography,
GC, as in force.
Method Code : 15 01 Λ11
Annex F1/H3 to the Certificate ESYD No. 142-3
19.11.2015
Tested materials/ products
Types of test/ Properties to be measured
8. Alcohol, spirit drinks, wine 1. Determination of alcoholic strength
9. Alcohol, spirit drinks
1. Determination of congeners
2. Determination of phthalates esters
3. Determination of the 14C content
10. Wine
11. Spirit drinks
1. Determination of total sulfur dioxide
1. Determination of sugars in spirit
drinks
Applied Standards/ Techniques to be
used
E.E.C. Reg. 625/2003, 2870/2000,
Electronic densimeters, Compendium of
International Methods of Wine and
Must Analysis OIV Edition 2012
Volume I (method OIV-MA-AS31201A),
Method Code : 1503 AΠ 4
E.E.C. Reg. 2870/2000,
Gas Chromatography
Method Code: 1503 AΠ 5
In house Method, based on J. AOAC
Int., 1995, 78(3), 730 by HPLC,
Method Code : 1503 AΠ 10
In house method based on
Method of Regulation (EC) 625/2003,
Liquid Scintillation Counter
Method Code: 1503 AΠ 9
Compendium of International Methods
of Wine and Must Analysis OIV Edition
2012 Volume II (method OIV-MAAS323-04A), Method Code : 1503 ΟΙ 1
RECUEIL DES METHODES D’
ANALYSE DES BOISSONS
SPIRITUEUSES, DES ALCOOLS, DE
LA FRACTION AROMATIQUE DES
BOISSONS, Alain Bertrand, 1ere
edition, 2011, OIV. HPLC,
Method Code: 1503 AΠ 11
13
12
12. Alcohol and spirit drinks, 1. Determination of C / C ratio by
isotope mass spectrometry (IRMS)
honey, sugar.
In-house Method, based on J. AOAC
Official Methods 978.17 και 991.41,
Reg. (EC) 440/2003, by EA- IRMS
Method Code: 1503 TΠ 1
13. Chilli peppers and chilli
products, mixes of spices,
sauces and snacks
1. Determination of Sudan Red dyes (I,
II, III, IV) content
In-house method based on Method of
European Commission
Notification 03/92 by HPLC,
Method Code: 1502 SUDAN
14. Foods soluble in water
(including drinks, jams,
syrups, confectionery)
1. Determination of water soluble food
colors content
In-house method by HPLC
Method Code: 1502 COLOURS
15. Bakery products,
beverages, non-alcoholic
drinks, juices and other fruit
drinks, marmalades, jams,
tomato products, vegetable
and fruit products, salad
dressings and sauces
1. Determination of Benzoates and
Sorbates content
Page 3 of 6
In-house method based on Method §
35,00.00-9 of LMGB (Lebensmittel
BundesGesetz) by HPLC
Method Code: 1502 SaBa-1
Annex F1/H3 to the Certificate ESYD No. 142-3
19.11.2015
Tested materials/ products
Types of test/ Properties to be measured
16. Bakery wares (bread,
1. Determination of Propionates content
bread type products, cakes,
pies, mixes for bakery wares)
Applied Standards/ Techniques to be
used
In-house method based on Method 35,
L-17.00 LMBG (Lebensmittel Bundes
Gesetz) by Gas Chromatography
Method Code: 1502 PROP-1
In-house method based on Method D3
17. Food of plant origin
1. Determination of Sulphur dioxide and
of Greek Food Code Part B’ by
(Vegetable and fruit products, Sulphites content
titrimetry
syrups, food stimulants)
Method Code: 1502 S-1
18. Cereals and cereal
products
1. Determination of Moisture content
2. Determination of Ash content
19. Honey
1. Determination of Hydroxymethylfurfural
In-house method based on Method of
Regulation EC 824/2000 (L100,
20.04.2000), Annex IV and Greek Food
Code, Part B', Method IB2 and ISO
712- 2009 by Gravimetry
Method Code: 1502 Y-1
In-house method based on Method of
Regulation EU 234/2010 (L72/320.03.2010), Annex II and Greek Food
Code, Part B', Method IB1 and ISO
2171- 2007 by Gravimetry
Method Code: 1502 T-900
In house Method, based on Harmonised
Methods of the International Honey
Commission (2002), Method 5.2, by
Spectrometry,
Method Code: 1503 M 9
In house Method, based on Harmonised
2. Determination of diastase activity with
Methods of the International Honey
Phadebas
Commission (2002), Method 6.2,
by Spectrometry,
Method Code: 1503 M 7
3. Determination of sugars
4. Determination of thyme pollen grains
in honey
In house Method, based on Harmonised
Methods of the International Honey
Commission (2002), Method 7.2,
by HPLC,
Method Code: 1503 M5
In house Method, based on Methods of
the International Honey Commission
[Apidologie 35 (Suppl.1) (2004)], by
Microscope
Method Code: 1503 M 16
Documented in-house multi-residue
Pesticide residues determination using
methods based on the Document
flexible scope protocol of the analytes of
SANCO 12571/2013 of the European
the categories:
Matrix categories
Commission
organochlorines,organophosphates,
(as defined in ESYD
using the following Analytical
Guideline for flexible scope - triazines, pyrethroids, carbamates,
Techniques:
ESYD ΟΕ1-KOneonicotinoids, triazoles, dinitroanilines,
ΕΥΕΛ/01/00/29.11.2011 amides, strobilourines, benzimidazoles,
(a) LC-MS-MS
and EU Regulations 396/2005
aryloxy-alkanoxy
acids
and
(b) GC-FPD-NPD
and 178/2006)
(c) GC-MS-MS
miscellaneous, given in the scope of
(d) GC-ECD
20. FOOD and WATER
matrices
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Annex F1/H3 to the Certificate ESYD No. 142-3
19.11.2015
Tested materials/ products
Types of test/ Properties to be measured
20.1. Fruit and vegetables
with high water content
(fruiting vegetables, leafy
vegetables, root and tuber
vegetables, citrus fruits et
al.)
accreditation (List of Accredited
Activities within flexible scope of
accreditation: STANDARD FORM : 15
05 5.04 01)**
20.2. Cereals, pulses
20.3. Vegetable oils and fats
http://www.esyd.gr/pweb/s/20/fil
es/kanonismoi/diadikasies/list_15
05 5.04 01.doc
20.4. High fat products of
animal origin: milk and
milk products, meat and
meat products, eggs,
fisheries products et al.
20.5. High sugar and low
water content
Applied Standards/ Techniques to be
used
and Flexible Scope SOP :
SOP 15 05 5.04 04/FLEX
20.6. Water (potable, surface
and groundwater intended
or not for human
consumption)
20.7. Wines
21. Water and ‘soda-like’
non-alcoholic beverages
Determination of dissolved hexavalent
chromium
22. Drinking and surface
waters
Determination of Cd, Pb, Cu, Cr, and Ni. Method 04 01 AAS W-1: In house
method with atomic absorption
spectrometry / graphite furnace.
23. Drinking water
Determination of bromates
24. Drinking water, surface
water and ground water of
low pollution
Page 5 of 6
Method 04 01 IC-BRO: In house
method with ion chromatography / post
column derivatisation / UV/Vis
spectrophotometry
1. Determination of dissolved anions (F, In house, Ion Chromatographic Method
Cl, Br, NO3, PO4, SO4, NO2) and cations (14-IC-W)
(Na, K, Ca, Mg)
2. Determination of dissolved Pb, As
25. Drinking water, surface
water and ground water
Method 04 01 IC CHRO: In house
method with ion chromatography / post
column derivatisation / UV/Vis
spectrophotometry based on method
EPA 218.6
In house, Atomic Absorption
Spectometry – Graphite Furnace
Method
Method AAS-As,Pb-W
1. Determination of Total Organic
Carbon (T.O.C.)
In house, Catalytic Oxidation – IR
Spectrometric Method (TOC-W)
2. Determination of dissolved Cu
In house, Atomic Absorption
Spectometry – Graphite Furnace
Method (AAS-Cu)
Annex F1/H3 to the Certificate ESYD No. 142-3
19.11.2015
Tested materials/ products
Types of test/ Properties to be measured
Applied Standards/ Techniques to be
used
In house, Inductively coupled plasma
3. Determination of dissolved Cr, Mn, Ni, atomic emission spectroscopy Method
Fe and Al
(ICP-OES-W)
Microbiological tests
1. Drinking water, surface
water, ground water and
swimming pool water
1.Enumeration of culturable
microorganisms at 22 and 37 oC
ISO 6222: 1999
2. Detection and enumeration of
Pseudomonas aeruginosa
ISO 16266:2006
2. Drinking water, surface
1. Detection and enumeration of
water, ground water,
intestinal enterococci
swimming pool water and sea
water
2. Detection and enumeration of
Coliforms and Escherichia coli
3. Drinking water, surface
water, ground water
ISO 7899-2: 2000
ISO 9308-1:2014
1. Detection and enumeration of
Clostridium perfringens
Annex ΙΙΙ of the J.M.D. Y2/2006/2001
as modified, completed and valid until
now
2. Detection and enumeration of the
spores of sulfite-reducing anaerobes
(clostridia)
ISO 6461-2:1986
Site of assessment: Laboratory permanent premises: 16 A. Tsoxa str. , 11521 Athens, Greece.
Approved signatories: D.Tsipi, T.Klada, V. Tzamtzis, I. Gardikis, N.Zoulis, C. Rizou, D. Chrysafidis,
S.Kyriakidis, T.Markidis, N.Nikolis, E.Papavasileiou, E.Billa, S.Iossifidou, O.Robokou, G.Karygiannis,
A.Arvanitaki, Chr.Sakellariou, Κ. Βergele, Z.Plyta, T.Kourouli, E.Botitsi, S.Antoniou, A. Constantinou,
E. Moschona, G. Spiliopoulos, C.Vakka, G.Karanikolopoulos.
This Scope of Accreditation replaces the previous of the annex F1/X3 dated 26.08.2013, F1/IX2 dated 2.09.2014,
F1/H2 dated 8.01.2014 except for 15. “Materials and articles intended to come into contact with food” and F1/X17
dated 11.02.2014.
The validation of the Accreditation Certificate No 142-3, to ELOT EN ISO/IEC 17025:2005, has been
extended up to 06.05.2016 by decision of E.SY.D’s National Accreditation Council.
Athens, November 19th, 2015
Ioannis Sitaras
Director of the Laboratories Accreditation Division
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Annex F1/H3 to the Certificate ESYD No. 142-3
19.11.2015