MANOLOPOULOU EUGENIA GENERAL INFORMATION Year of birth : Nationality : Profession : Marital status : 1960 Greek Agriculturist Married, two children Studies 1978-1984 : Agricultural University of Athens, Department of Food science and Technology PREVIOUS EXPERIENCE 1985-1986 : SYNERGAL Ltd: Responsible for the department of new products development.During this employment I was occupied in an European union financed project related with “ the development and optimization of the “strained yogurt”. 1986-today : Agricultural University of Athens, Department of Food Science and Technology, Laboratory of Dairy Research, Group of Dairy Microbiology. During this employment I am occupied in E.U. financed programs, related with isolation, identification, study of physicochemical and technological characteristics and preservation of dairy cultures. Scientific Activities a) Research E.U. programms -BAP (Biotechnology Action Program) BAP-0143-GR (1986-1989) Creation of a lactic acid cultures collection. Modelling and control techniques of thermophilic mixed cultures -MINE (Microbial Information Network in Europe) (1988- ) -BRIDGE (Biotechnology Research for Innovation, Development and Growth in Europe) LAB-T-Project BIOT-CT91-0263 (1991-1994) Improvement and exploitation of lactic acid bacteria for biotechnology purposes -ECLAIR (European Collaborative Linkage of Agriculture and Industry through Research) AGRE-CT91-0064 (1991-1994) Isolation of new starter strains of lactic acid bacteria from cheese and fermented milks b) Research Programms of GGET : 1- ΕΠΕΤ II (1995-1998) Standardisation of Greek traditional cheeses 2- ΠΑΒΕ (1995-1997) Development of new dairy products, using wild lactic acid bacteria strains c) Catalogue of Culture Collection ACA-DC 1. “Greek Co-ordinated Collection of Microorganisms”(GCCM), Athens, 1993. 2. Update of databank “ACA-DC ”. Publications 1. G.Kalantzopoulos, E. Tsakalidou and E. Manolopoulou 1990 Proteinase, peptidase and esterase activities of cell-free extracts from wild strains of Lactobacillus delbreuckii subsp. bulgaricus and Streptococcus salivarius subsp. thermophilus isolated from traditional Greek yoghurt. J. Dairy Res. 57, 593-601 2. E. Tsakalidou, E. Manolopoulou, B. Tsilibari, M. Georgalaki and G. Kalantzopoulos 1993 Esterolytic activities from Enterococcus faecium and Enterococcus durans strains. Neth. Milk Dairy J. 47, 145-150 3. E. Tsakalidou, E. Manolopoulou, E. Kabaraki, E. Zoidou, B. Pot, K. Kersters, and G. Kalantzopoulos 1994 The combined use of whole cell protein extracts for the identification (SDS-PAGE) and enzyme activity screening of lactic acid bacteria isolated from traditional Greek dairy products. System. Appl. Microbiol. 17, 444-458 4. E. Tsakalidou, R. Anastasiou, C. Papademetriou, E. Manolopoulou and G. Kalantzopoulos 1998 Purification and characterization of an intracellular X-prolyldipeptidyl aminopeptidase from Streptococcus thermophilus strain ACA-DC 4. J. Biotechnol. 59, 203-211 5. Papadelli M., E. Manolopoulou, G. Kalantzopoulos and E. Tsakalidou 2003 Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridisation. Int. J. Food Microbiol 81, 233-241 6. E. Manolopoulou, P. Sarantinopoulos, E. Zoidou, A. Aktypis, E. Moschopoulou, I. Kandarakis, G. Kalantzopoulos. Evolution of Feta cheese microbial flora, during traditional manufacture and ripening. Int. J. of Food Microbiol82 (2003)153-161. 7. Ρ. Αναστασίου, Μ. Γεωργαλάκη, Ε. Μανωλοπούλου, Vim De Malsche, Δ. Ψύρρα, Luc De Vuyst και Ε., Tσακαλίδου 2005. Ο Streptococcus macedonicus ACA-DC 198, ως προστατευτική καλλιέργεια στην παραγωγή του τυριού Κασέρι. Περιοδικό Γαλακτοκομία, Δεκέμβριος 2005. 8. Georgalaki M.D., A. Aktypis, E. Manolopoulou, A. Gavriel, M. Papadelli and E. Tsakalidou (2005) Induction of macedocin biosynthesis, a lantibiotic produced by Streptococcus macedonicus ACA-DC 198. 8th FEMS Symposium on Lactic Acid Bacteria, Egmond aan Zee, The Netherlands. 9. E. Μανωλοπούλου, Σ. Γιουλάτος, Α. Ακτύπης, Ε. Τσακαλίδου και Γ. Καλαντζόπουλος 2006. Η συμβολή των μικροβιακών συλλογών στη διατήρηση της βιοποικιλότητας. Το παράδειγμα της συλλογής ACA-DC του Εργαστηρίου Γαλακτοκομίας.. Hλεκτρονική έκδοση περιοδικού Εθνικής Επιτροπής Γάλακτος, τεύχος 10 2006 http://www.aua.gr/ndcg/teuxos1.pdf. 10. R. Anastasiou, M. Georgalaki, E. Manolopoulou, I. Kandarakis, Luc De Vust, E. Tsakalidou. The performance of Streptococcus macedonicus ACA-DC 198 as starter culture in Kasseri cheese production. Int. Dairy Journal 17(2007) 208-217. 11. M. Georgalaki, E. Manolopoulou, R. Anastasiou, M. Papadelli, E. Tsakalidou. Detection of Streptococcus macedonicus in Greek cheeses. International Dairy Journal vol 19(2) (2009) 96-99. COMMUNICATIONS 1. BRIDGE T-PROJECT CEC : «Biotechnology of Lactic Acid Bacteria» 3nd Meeting of Contractors in Athens, 1993. 2. Kalantzopoulos G, E. Manolopoulou, A. Aktypis and E. Tsakalidou The ACADC Greek Collection of Microorganisms. Conference of the European Culture Collections Organization, Crete, Greece,2001. 3. Papadelli M., E. Manolopoulou, G. Kalantzopoulos and E. Tsakalidou. Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridization. 53nd Meeting of the Hellenic Society of Biochemistry and Molecular Biology, Athens,2001. 4. Papadelli M., E. Manolopoulou, G. Kalantzopoulos and E. Tsakalidou. Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridization. 7th Symposium on Lactic Acid Bacteria, Egmond aan Zee, The Netherlands,2002. 5. R. Anastasiou, D. Kousta, E. Manolopoulou, M. Georgalaki, I. Kandarakis and E. Tsakalidou. Streptococcus macedonicus ACA-DC 198 and its performance as adjunct starter in Kasseri cheese production. IDF symposium on cheese: Ripening, characterization and Technology Praque, Check. Republic,2004. 6. Ι. Kandarakis, T. Massouras, E. Manolopoulou, D. Karagiorgos, N. Kritikos, E. Anyfantakis 2005. Study of physicochemical composition and nutrient profile of traditional graviera of Paros. Mediet 2005.. 7. E. Manolopoulou, M. Georgalaki, R. Anastasiou, M. Papadelli, A. Aktypis, G. Zoumpopoulou, P. Maragoudakis, I. Asteri, K. Papadimitriou, G. Kalantzopoulos and E. Tsakalidou The ACA-DC Greek Collection of microorganisms. The 11th International Conference on Culture Collections, Gosslar, Germany,2007. 8. Ε. Manolopoulou, M. Georgalaki, R. Anastasiou, M. Papadelli, A. Aktypis, G. Zoumpopoulou, P. Maragoudakis, I. Asteri, K. Papadimitriou, G. Kalantzopoulos and E. Tsakalidou. Τhe ACA-DC Greek Collection of Microorganisms. 10 Eθνικό Συνέδριο της Επιστημονικής Εταιρείας Μικροβιόκοσμος, με τίτλο «Ο ανεξερεύνητος φυσικός μας πλούτος», Αθήνα, 2008. 9. M. Papadelli, I. Lytra, G. Zoumpopoulou, E. Manolopoulou, M. Georgalaki, R. Anastasiou, E. Tsakalidou. The performance of Leuconostoc mesederoides subsp. mesederoides strain Lm139 as starter in Greek-style kalamon olives fermentation. 22nd International ICFMH Symposium Food Micro 2010. 2. 3. 4. 5. 6. 7. 1. Papadelli M, E. Manolopoulou, G. Kalantzopoulos and E. Tsakalidou 2003. Rapid detection and identification of Streptococcus macedonicus by species-specific PCR and DNA hybridisation. Int. J. Food Microbiol 81, 233-241 E. Manolopoulou, P. Sarantinopoulos, E. Zoidou, A. Aktypis, E. Moschopoulou, I. Kandarakis, G. Kalantzopoulos 2001. Evolution of Feta cheese microbial flora, during traditional manufacture and ripening. Int. J. of Food Microbiol82 (2003)153-161. Georgalaki M.D., A. Aktypis, E. Manolopoulou, A. Gavriel, M. Papadelli and E. Tsakalidou (2005) Induction of macedocin biosynthesis, a lantibiotic produced by Streptococcus macedonicus ACA-DC 198. 8th FEMS Symposium on Lactic Acid Bacteria, Egmond aan Zee, The Netherlands. R. Anastasiou, M. Georgalaki, E. Manolopoulou, I. Kandarakis, Luc De Vust, E. Tsakalidou. The performance of Streptococcus macedonicus ACA-DC 198 as starter culture in Kasseri cheese production. Int. Dairy Journal 17(2007) 208-217. M. Georgalaki, E. Manolopoulou, R. Anastasiou, M. Papadelli, E. Tsakalidou. Detection of Streptococcus macedonicus in Greek cheeses. International Dairy Journal vol 19(2) (2009) 96-99. M. Papadelli, I. Lytra, G. Zoumpopoulou, E. Manolopoulou, M. Georgalaki, R. Anastasiou, E. Tsakalidou. The performance of Leuconostoc mesederoides subsp. mesederoides strain Lm139 as starter in Greek-style kalamon olives fermentation. 22nd International ICFMH Symposium Food Micro 2010.