Department of Medical Biochemistry
MEDICAL BIOCHEMISTRY II
General Medicine-International students
2nd Year, Summer semester, Academic year 2013/2014
Academic
weeks
Date
1st
Feb
10/13
2014
2nd
Feb
17/20
2014
3rd
Feb
24/27
2014
4th
Mar
03/06
2014
5th
Mar
10/13
2014
LECTURES
Teacher
Nucleotides metabolism
Protein metabolism
- Protein digestion and absorption;
- Urea cycle. Regulation, transport and detoxification of ammonia;
- Amino acids in the intermediate metabolism.
Protein metabolism
Metabolism of individual amino acids
- Amino acids special metabolites.
Vitamins, role in the metabolism
- Vitamins as coenzymes and their significance in the metabolic pathways;
- The effect of hypovitaminosis on the metabolism in different tissues.
Tetrapyroles metabolism, jaundice types
- Hemoglobin synthesis and degradation, regulation;
- Biochemistry of jaundice.
Carbohydrate, proteins and lipids metabolic interrelationships. Nutrition
- Organs metabolic interrelationships.
Carbohydrate, proteins and lipids metabolic interrelationships:
obesity, stress, pregnancy, lactation, starvation, aging, exercise, vegetarian diet.
Biochemical basis of the diabetes mellitus
Atherosclerosis
- Biochemical and molecular aspects of atherosclerosis and the other civilization diseases.
1st CREDIT TEST
1
6th
7th
Mar
17/20
2014
Mar
24/28
2014
8th
Mar/Apr
31/03
2014
9th
Apr
07/10
2014
10th
Apr
14
2014
11th
Apr
24
2014
Cell signaling, signal molecules
Hormones and neurohormonal regulation
- Relationship between the hypotalamus, the anterior pituitary and target organs.
Steroid hormones
Hormones of the thyroid and parathyroid glands
Peptide hormones
Free radicals in the living system
- Production of reactive oxygen/nitrogen species in cells;
- Oxidative damage to lipids, proteins and DNA;
- Antioxidant systems;
- Oxidative stress in various pathological states.
Neurochemistry, neurotransmitters, synapse
Plasma enzymes and their diagnostic importance, isoenzymes
Blood proteins, erythrocyte metabolism
- Blood coagulation and fibrinolysis.
Minerals, role in the metabolism
- Transformation of Na+, K+, Cl-;
- Transformation of Ca2+, Mg2+, PO43-, regulation, parathyrin, calcitonin, calcitriol;
- Transformation of Fe2+, transport proteins, receptors;
- Transformation of Cu , Zn, Mn, Se, Cr and another trace elements.
Biochemical function of the kidney, urine production
Acid-base balance. Buffer systems
- Regulation of acid-base balance;
- Metabolic acidosis and alkalosis;
- Respiratory acidosis and alkalosis.
2
12th
Apr
28
2014
Connective tissue biochemistry
Muscle biochemistry
- Metabolism of muscle contraction;
- Effect of hormones and regulation of muscle biochemistry.
2nd CREDIT TEST
13th
May
05
2014
Biochemical functions of the liver
- Endogenous and exogenous functions of the liver;
- Xenobiochemistry and biotransformation reactions.
The neurodegenerative diseases, metabolic disorders of compound lipids
14th
May
12/15
2014
Biochemistry of immune system
The select functions of plasmatic enzymes
15th
May
19/22
2014
The compensatory lecture
3
Academic
weeks
Date
1st
Feb
10-14
2014
2nd
3rd
4th
5th
Feb
17-21
2014
Feb
24-28
2014
Mar
03-07
2014
Mar
10-14
2014
Seminars [S] – Lab. practicals [LP] – PP presentations [PPT]
S: Integration of carbohydrate and lipid metabolism. Regulation.
LP: Estimation of proteins in serum by the biuret reaction.
S: Metabolisms of purines and pyrimidines nucleotides. Regulation.
LP: Estimation of the uric acid in serum.
PPT:
1. Genetic defects of purines metabolism, basic divide.
2. Role of antimetabolites in medicine.
S: Metabolism of proteins. Urea metabolism. Nitrogen fixation. Glutamine cycle.
LP: Estimation of urea in serum.
PPT:
1. Hyperammonemia and ammonia toxicity.
2. Glutamine as an alternative substrate for extrahepatic tissues.
3. Pathological conformational changes in proteins – relationship to particular diseases.
S: Amino acids metabolism, inborn error of the amino acids metabolism.
P: Production of creatin from amino acids and detection of creatinine in serum.
PPT:
1. Metabolism of sulfur containing amino acids in connection with some pathological
processes.
2. Metabolic diseases of catabolism of aromatic amino acids.
3. Amino acids as signal molecules.
S: Vitamins, role in the metabolism. Hypovitaminosis.
PPT:
1. Nicotinic acid (niacin) and metabolism of lipoproteins.
2. Antioxidative vitamins and their conjugation to metabolism.
3. Osteoporosis and calciferols.
4. Deficit of folic acid in some pathologically diseases.
1st CREDIT TEST
Teacher
Assoc. Prof. RNDr. Peter
RAČAY, PhD.;
prof. RNDr. Ján
LEHOTSKÝ, D.Sc.
prof. RNDr. Peter
KAPLAN, PhD.
Mgr. Radovan MURÍN,
PhD.
prof. RNDr. Peter
KAPLAN, PhD.
Assoc. Prof. RNDr. Peter
RAČAY, PhD.
Mgr. Radovan MURÍN,
PhD.
prof. RNDr. Ján
LEHOTSKÝ, D.Sc.
Assoc. Prof. Mgr. Eva
BABUŠÍKOVÁ, PhD.
Ing. Zuzana TATARKOVÁ,
PhD.
4
6th
7th
8th
9th
Mar
17-21
2014
Mar
24-28
2014
Mar/Apr
31-04
2014
Apr
07-11
2014
S: Hemoglobin metabolism. Tetrapyroles.
P: Bilirubin estimation in plasma.
PPT:
1. Hyperbilirubinemia connected with increased production of unconjugated bilirubin.
2. Biochemical diagnostic tests for liver diseases.
3. Secretion of bile and its failure.
S: Metabolic interrelationships, nutrition. Energy metabolism during the fasting and overweight.
P: Estimation of transaminase activity in serum.
PPT:
1. Biochemical aspects of obesity and stress.
2. Metabolism during aerobic and anaerobic exercise.
3. Function of different organs in the metabolic interrelationship.
4. Metabolic interrelationship during aging and pregnancy.
S: Diabetes mellitus, atherosclerosis.
P: Estimation of glucose by glucometer. Oral glucose tolerance test.
PPT:
1. OxyLDL and atherosclerosis.
2. Incretins and their utilization in pharmacology.
3. Non-enzymatic glycation of proteins.
4. Metabolism of proteoglycans and diabetes mellitus.
S: Metabolic pathways of hormonal regulation.
P: Estimation of 17-oxosteroids in urine.
PPT:
1. Parameters describing thyroid function.
2. Ovary and regulation of reproduction cycle.
3. Function of IP3 in metabolism.
4. Role of catecholamines in the heart.
Assoc. Prof. Mgr. Eva
BABUŠÍKOVÁ, PhD.
Ing. Zuzana TATARKOVÁ,
PhD.
Mgr. Radovan MURÍN,
PhD.
Assoc. Prof. Mgr. Eva
BABUŠÍKOVÁ, PhD.
Ing. Zuzana TATARKOVÁ,
PhD.
prof. RNDr. Ján
LEHOTSKÝ, D.Sc.
5
10th
11th
Apr
21-25
2014
12th
S: Plasma enzymes and proteins. Importance for clinical diagnostics.
PL: Determination of GMT in serum. Estimation of the urine and an unknown sample.
PPT:
1. Role of cytokines in the metabolism.
2. Role of proteins in inflammation.
S: Plasma enzymes and proteins. Importance for clinical diagnostics.
PL: Determination of GMT in serum. Estimation of the urine and an unknown sample.
PPT:
1. Role of cytokines in the metabolism.
2. Role of proteins in inflammation.
S: Minerals metabolism.
P: Iron and transferrin estimation in the blood.
PPT:
1. Imbalance of calcium homeostasis.
2. Imbalance of potassium homeostasis.
3. The role of chrome, selenium and manganese in cells.
Assoc. Prof. Mgr. Eva
BABUŠÍKOVÁ, PhD.
Ing. Zuzana TATARKOVÁ,
PhD.
Assoc. Prof. Mgr. Eva
BABUŠÍKOVÁ, PhD.
Ing. Zuzana TATARKOVÁ,
PhD.
Mgr. Radovan MURÍN,
PhD.
2nd CREDIT TEST
13th
14th
15th
May
05-09
2014
May
12-16
2014
May
19-23
2014
S: Acid-base balance. Biochemical functions of the kidney, urine production.
(Seminar is realize on the Department of Clinical Biochemistry in Hospital)
Retake of credit tests
Compensatory laboratory practicals, credits
prof. Dušan DOBROTA,
M.D., PhD.;
Daniel ČIERNY, M.D.
RNDr. Jozef HATOK, PhD.
RNDr. Jozef HATOK, PhD.
6
REQUIREMENTS FOR CREDITS
General Medicine-International students
nd
2 Year, Summer semester, Academic year 2013/2014
Department of Medical Biochemistry provides these rules to grant 9 credits for the subject “Medical Biochemistry” in summer semester in conformity with
Act 131/2002 Coll. and Study regulation of Comenius University in Bratislava, Jessenius Faculty of Medicine in Martin, Article 22 and Article 23.
1. 100 % attendance at seminars and practical exercises. All absences due to diseases must be excused by a medical confirmation and substitute in
the substitution week (one excused absence is without any substitution). Maximal number of absences is 3. During the time of a seminar/lab.
practical (substitution week) only one seminar/lab. practical can be substituted at that time.
2. During the semester are written two credit tests. From each of the test 60 % of total score points is necessary. Maximal number of points for the
one test is 50. It is possible to obtain 100 points from two credit tests and 10 points from a student presentation. Credit tests can be substituted
maximally two times.
3. Exam from the subject “Medical Biochemistry” is composed from two parts, written and oral*. Written part is one or two days before the oral part
and the test consists of 40 questions. 60 % of total score points is necessary for continuing in the oral part of the exam. Final evaluation of the exam
consists from the results of oral and written parts as well as total results from the summer semester.
4. *If a student obtained from both tests and presentations classification “A” – will not do the written test, only oral form of exam!
Study and test results:
A (1)
100 – 91 %
B (1.5)
90.9 – 81 %
C (2)
80.9 – 73 %
D (2.5)
72.9 – 66 %
E (3)
65.9 – 60 %
Fx
less than 60 %
100 – 91 points
90 – 81 points
80 – 73 points
72 – 66 points
65 – 60 points
59 and less points
7