CURRICULUM VITAE
NAME:
Mohammed A. Abdel-Ghani
NATIONALITY:
Egypt
ADDRESS:
Department of Theriogenology, faculty of veterinary medicine, Assuit
University, Assuit, Egypt
Phone:
(+2) 010-2530-8772
E-mail:
mohammed_ali76@hotmail.com
EDUCATION:
PhD
The United Graduate School of Veterinary Sciences, Gifu University. (Japan). Major:
Reproductive Biotechnology. 2008-2012
MVSc
University of Assuit, Assuit (Egypt). Major: Theriogenology. 2001-2005
BVSc (Hons.)
Faculty of Veterinary medicine, Assuit (Egypt). 1995-2000.
KEY QUALIFICATIONS:
Areas Of Expertise: Reproductive physiology, gamete biology, cryopreservation, embryos,
oocytes, xenotransplantation, semen
Dr. Abdel-Ghani is a Research Scientist (Lecturer), Department of Theriogenology, Faculty of Veterinary
Medicine, Assuit University, Assuit, Egypt. During his career, he has worked on male and female
infertility (physiology and pathology of reproductive organs), sexual behavior, pubertal development,
semen quality improvement, cryopreservation of semen, cryopreservation of oocytes, cryopreservation of
ovarian tissue, cryopreservation of testicular tissue, In vitro maturation of oocytes, in vitro fertilization, In
vitro embryo culture, cryopreservation of embryos.
Dr. Abdel-Ghani taught Animal reproduction including Gynecology, Andrology, Obstetrics and
Artificial Insemination to undergraduate students at University of Assuit (2000 and 2008).
TEACHING EXPERIENCE:

Student, Faculty of veterinary medicine, Assuit University. Assuit, Egypt. 1995-2000.

Demonstrator, Department of Theriogenology, faculty of Veterinary Medicine, Assuit University,
Assuit, Egypt. 2000-2005.

Assistant Lecturer, Department of Theriogenology, faculty of Veterinary Medicine, Assuit
University, Assuit, Egypt. 2006-2012.

o
Taught Andrology and Gynecology to 3th and 4th grade V.M students.
o
Taught Obstetrics and artificial insemination to 5th grade V.M students.
Lecturer, Department of Theriogenology, faculty of Veterinary Medicine, Assuit University, Assuit,
Egypt. 2012
o
Taught Andrology and Gynecology to 3th and 4th grade V.M students.
o
Taught Obstetrics and Artificial Insemination to 5th grade V.M students.
o
Taught Andrology, Gynecology, Obstetrics, Artificial Insemination, and Reproductive
Biotechnology to Doctoral course students.
RESEARCH EXPERIENCE:

PhD, The United Graduate School of Veterinary Sciences, Gifu University. (Japan). September 2012.
o
Conducting research on the in vitro maturation of oocytes, embryos transfer, in vitro
organ culture, Xenotransplantation to NOD-SCID mice.
o

Conducting research on the cryopreservation of gametes, embryos, testes and ovaries.
Demonstrator, Department of Theriogenology, faculty of Veterinary Medicine, Assuit University,
Assuit, Egypt. 2000-2005.
o
Conducted research to the use of real time ultrasonography for diagnosis of infertility
problems in Egyptian cattle and buffaloes.

B. V Sc.
with grade (very good with honor), Faculty of veterinary medicine, Assuit University.
Assuit, Egypt. 2000.
TECHNICAL SKILLS:
Cryopreservation of gametes; In vitro fertilization of oocyte (oocyte maturation, in vitro sperm
capacitation, and fertilization); Embryo virification; Gametes survival assays; Embryo survival assays;
Cryopreservation of ovarian tissue; Cryopreservation of testicular tissue; nuclear status assays (using
Propidium Iodide and Hoechest stains); Intracytoplasmic sperm injection; In vitro embryo culture;
Embryo transfer using Cystoscope. In vitro organ culture; Computer-assisted sperm motion analysis
(CASA).
Xenotrasnplantation of ovarian tissue ovarian tissue into NOD-severe combined immune deficiency
(SCID) mice (transplanted under kidney capsule, into ovarian bursa, back muscle, and subcutaneously).
Fluorescence in situ hybridisation (FISH); Polymerase chain reaction (PCR); Immunohistochemistry.
DNA and RNA extraction. Elisa for hormonal analysis.
Ultrasonography for diagnosis of infertility problems; Pregnancy diagnosis; Sex determination;
Obstetrical operations (Cesarean section, uterine torsion, uterine prolapsed, and dystocia).
ACADEMIC HONORS:

Scholarship was awarded for PhD studies in Japan by the Ministry of Egyptian Higher Education –
from 2008 – 2012, on securing the highest grade point average in M.Sc. in all faculties of University
of Assuit, Assuit (Egypt).
PROFESSIONAL MEMBERSHIPS:

Japan Society for Reproductive Medicine (Japan).

The Egyptian Society for Reproduction (Egypt).

The Egyptian Society for Cattle Diseases (Egypt).

The Egyptian Society of Animal Production (ESAP) (Egypt).
REFERENCES:
Dr. Hiroshi Suzuki, Professor, Research Unit for Functional Genomics, National Research Center for
Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, 0808555, Japan. The United Graduate School of Veterinary Sciences, Gifu University. (Japan). Phone: (+81)
155-49-5640. Email: hisuzuki@obihiro.ac.jp
Dr. Kazuyoshi Hashizume Professor, Department of veterinary science, Faculty of Agriculture, Iwate
University, Ueda-3-18-8, Morioka, Iwate, 020-8550. Japan. Phone: (+81) 019-621-6210. Email:
Kazuha@iwate-u.ac.jp
Dr. Takashi Shimizu, Associate Professor, Animal Reproduction Science, Graduate School of Animal
and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, 0808555, Japan. Phone: (+81) 155-49-5640. Email: shimizut@obihiro.ac.jp
PUBLICATIONS
Papers Published in Refereed Journals:
1. Abdel-Ghani, M. A. Shimizu, T. Suzuki, H. (2014): Expression Pattern of Vascular
Endothelial Growth Factor (VEGF) in Canine Folliculogensis and its Effect on the Growth
and Development of Ovarian Follicles after Organ Culture. Reprod. Dom. Anim. 49, 734–
739.
2. Abdel-Ghani, M. A. Suzuki, H. (2014): Effect of cumulus cells on canine oocyte
vitrification using DAP213 cryotop method. J. Asuit Vet. Med. 60, 14: 47-54.
3. Abdel-Ghani, M. A. Shimizu, T. Asano, T. Suzuki, H. (2012): In vitro maturation of canine
oocytes co-cultured with bovine and canine granulosa cell monolayers. Theriogenology
77, 2, 347-355.
4. Abdel-Ghani, M. A. Abe, Y. Asano, T. Hamano, S. Suzuki, H. (2011): Effect of bovine
cumulus-oocyte complexes conditioned medium on in vitro maturation of canine oocytes.
Reprod. Med. Biol. 10, 1: 43-49.
5. Abdel-Ghani, M. A. Abe, Y. Asano, T.
H. Suzuki (2011): Effect of graft site and
gonadotrophin treatment on follicular development of canine ovarian grafts transplanted
to NOD-SCID mice. Reprod. Med. Biol., 10, 4: 259-266.
6. Abe, Y. Suwa, Y. Asano, T. Ueta, Y. Kobayashi, N. Ohshima, N. Shirasuna, S. AbdelGhani, M. A. Oi, M. Kobayashi, Y. Miyoshi, M. Miyahara, K. Suzuki, H (2011):
Cryopreservation of Canine Embryos. Biol. Reprod. 84, 2: 363-368.
7.
Abe, Y. Asano, T. Abdel-Ghani, M. A. Suzuki, H. (2010): Vitrification of canine cumulusoocyte complexes in DAP213 with a cryotop holder. Reprod. Med. Biol. 9, 2: 115-120.
Papers/Abstracts Published in Proceedings:
8. Mohammed A. Abdel-Ghani: Changes in blood flow, progesterone, estradiol-17β, nitric oxide
after estrus synchronization during breeding season of sa'idi goats. The International Conference
of Animal Production. Pp 25 (2015, April, El-Harghda, Egypt).
9. Mohammed A. Abdel-Ghani: Effects of treatment with GDF-9 and VEGF on the progression and
survival of follicles in cryopreserved canine ovarian tissues in culture. The European Veterinary
Conference "Voorjaarsdagen". (2014, April, Amsterdam, Netherland).
10. Mohammed A. Abdel-Ghani: Nuclear competence profile of canine oocytes with/ without direct
contact between canine oocytes and bovine granulosa cell monolayers. The 24th Egyptian society
conference for animal infertility. (2014, February, El-Harghda, Egypt).
11. Mohammed A. Abdel-Ghani: Effect of bovine cumulus–oocyte complexes conditioned medium on in
vitro maturation of canine oocytes. The 45th Southern European Veterinary Conference. Pp 8 (2010,
October, Barcelona, Spain).
12. Mohammed A. Abdel-Ghani: Influence of graft site and gonadotrophins on follicular development of
canine ovarian grafts transplanted to NOD-SCID mice: inference for canine ovarian xenograft. The
45th Southern European Veterinary Conference. Pp 9 (2010, October, Barcelona, Spain).
13. Mohammed A. Abdel-Ghani: The effect of bovine and canine granulose monolayers on in vitro
maturation of canine oocytes. The 150th Japanese Veterinary Meeting. Pp 263 (2010, September,
Obihrio, Japan).