CURRICULUM VITAE NAME: Mohammed A. Abdel-Ghani NATIONALITY: Egypt ADDRESS: Department of Theriogenology, faculty of veterinary medicine, Assuit University, Assuit, Egypt Phone: (+2) 010-2530-8772 E-mail: mohammed_ali76@hotmail.com EDUCATION: PhD The United Graduate School of Veterinary Sciences, Gifu University. (Japan). Major: Reproductive Biotechnology. 2008-2012 MVSc University of Assuit, Assuit (Egypt). Major: Theriogenology. 2001-2005 BVSc (Hons.) Faculty of Veterinary medicine, Assuit (Egypt). 1995-2000. KEY QUALIFICATIONS: Areas Of Expertise: Reproductive physiology, gamete biology, cryopreservation, embryos, oocytes, xenotransplantation, semen Dr. Abdel-Ghani is a Research Scientist (Lecturer), Department of Theriogenology, Faculty of Veterinary Medicine, Assuit University, Assuit, Egypt. During his career, he has worked on male and female infertility (physiology and pathology of reproductive organs), sexual behavior, pubertal development, semen quality improvement, cryopreservation of semen, cryopreservation of oocytes, cryopreservation of ovarian tissue, cryopreservation of testicular tissue, In vitro maturation of oocytes, in vitro fertilization, In vitro embryo culture, cryopreservation of embryos. Dr. Abdel-Ghani taught Animal reproduction including Gynecology, Andrology, Obstetrics and Artificial Insemination to undergraduate students at University of Assuit (2000 and 2008). TEACHING EXPERIENCE: Student, Faculty of veterinary medicine, Assuit University. Assuit, Egypt. 1995-2000. Demonstrator, Department of Theriogenology, faculty of Veterinary Medicine, Assuit University, Assuit, Egypt. 2000-2005. Assistant Lecturer, Department of Theriogenology, faculty of Veterinary Medicine, Assuit University, Assuit, Egypt. 2006-2012. o Taught Andrology and Gynecology to 3th and 4th grade V.M students. o Taught Obstetrics and artificial insemination to 5th grade V.M students. Lecturer, Department of Theriogenology, faculty of Veterinary Medicine, Assuit University, Assuit, Egypt. 2012 o Taught Andrology and Gynecology to 3th and 4th grade V.M students. o Taught Obstetrics and Artificial Insemination to 5th grade V.M students. o Taught Andrology, Gynecology, Obstetrics, Artificial Insemination, and Reproductive Biotechnology to Doctoral course students. RESEARCH EXPERIENCE: PhD, The United Graduate School of Veterinary Sciences, Gifu University. (Japan). September 2012. o Conducting research on the in vitro maturation of oocytes, embryos transfer, in vitro organ culture, Xenotransplantation to NOD-SCID mice. o Conducting research on the cryopreservation of gametes, embryos, testes and ovaries. Demonstrator, Department of Theriogenology, faculty of Veterinary Medicine, Assuit University, Assuit, Egypt. 2000-2005. o Conducted research to the use of real time ultrasonography for diagnosis of infertility problems in Egyptian cattle and buffaloes. B. V Sc. with grade (very good with honor), Faculty of veterinary medicine, Assuit University. Assuit, Egypt. 2000. TECHNICAL SKILLS: Cryopreservation of gametes; In vitro fertilization of oocyte (oocyte maturation, in vitro sperm capacitation, and fertilization); Embryo virification; Gametes survival assays; Embryo survival assays; Cryopreservation of ovarian tissue; Cryopreservation of testicular tissue; nuclear status assays (using Propidium Iodide and Hoechest stains); Intracytoplasmic sperm injection; In vitro embryo culture; Embryo transfer using Cystoscope. In vitro organ culture; Computer-assisted sperm motion analysis (CASA). Xenotrasnplantation of ovarian tissue ovarian tissue into NOD-severe combined immune deficiency (SCID) mice (transplanted under kidney capsule, into ovarian bursa, back muscle, and subcutaneously). Fluorescence in situ hybridisation (FISH); Polymerase chain reaction (PCR); Immunohistochemistry. DNA and RNA extraction. Elisa for hormonal analysis. Ultrasonography for diagnosis of infertility problems; Pregnancy diagnosis; Sex determination; Obstetrical operations (Cesarean section, uterine torsion, uterine prolapsed, and dystocia). ACADEMIC HONORS: Scholarship was awarded for PhD studies in Japan by the Ministry of Egyptian Higher Education – from 2008 – 2012, on securing the highest grade point average in M.Sc. in all faculties of University of Assuit, Assuit (Egypt). PROFESSIONAL MEMBERSHIPS: Japan Society for Reproductive Medicine (Japan). The Egyptian Society for Reproduction (Egypt). The Egyptian Society for Cattle Diseases (Egypt). The Egyptian Society of Animal Production (ESAP) (Egypt). REFERENCES: Dr. Hiroshi Suzuki, Professor, Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, 0808555, Japan. The United Graduate School of Veterinary Sciences, Gifu University. (Japan). Phone: (+81) 155-49-5640. Email: hisuzuki@obihiro.ac.jp Dr. Kazuyoshi Hashizume Professor, Department of veterinary science, Faculty of Agriculture, Iwate University, Ueda-3-18-8, Morioka, Iwate, 020-8550. Japan. Phone: (+81) 019-621-6210. Email: Kazuha@iwate-u.ac.jp Dr. Takashi Shimizu, Associate Professor, Animal Reproduction Science, Graduate School of Animal and Food Hygiene, Obihiro University of Agriculture and Veterinary Medicine, Inada, Obihiro, 0808555, Japan. Phone: (+81) 155-49-5640. Email: shimizut@obihiro.ac.jp PUBLICATIONS Papers Published in Refereed Journals: 1. Abdel-Ghani, M. A. Shimizu, T. Suzuki, H. (2014): Expression Pattern of Vascular Endothelial Growth Factor (VEGF) in Canine Folliculogensis and its Effect on the Growth and Development of Ovarian Follicles after Organ Culture. Reprod. Dom. Anim. 49, 734– 739. 2. Abdel-Ghani, M. A. Suzuki, H. (2014): Effect of cumulus cells on canine oocyte vitrification using DAP213 cryotop method. J. Asuit Vet. Med. 60, 14: 47-54. 3. Abdel-Ghani, M. A. Shimizu, T. Asano, T. Suzuki, H. (2012): In vitro maturation of canine oocytes co-cultured with bovine and canine granulosa cell monolayers. Theriogenology 77, 2, 347-355. 4. Abdel-Ghani, M. A. Abe, Y. Asano, T. Hamano, S. Suzuki, H. (2011): Effect of bovine cumulus-oocyte complexes conditioned medium on in vitro maturation of canine oocytes. Reprod. Med. Biol. 10, 1: 43-49. 5. Abdel-Ghani, M. A. Abe, Y. Asano, T. H. Suzuki (2011): Effect of graft site and gonadotrophin treatment on follicular development of canine ovarian grafts transplanted to NOD-SCID mice. Reprod. Med. Biol., 10, 4: 259-266. 6. Abe, Y. Suwa, Y. Asano, T. Ueta, Y. Kobayashi, N. Ohshima, N. Shirasuna, S. AbdelGhani, M. A. Oi, M. Kobayashi, Y. Miyoshi, M. Miyahara, K. Suzuki, H (2011): Cryopreservation of Canine Embryos. Biol. Reprod. 84, 2: 363-368. 7. Abe, Y. Asano, T. Abdel-Ghani, M. A. Suzuki, H. (2010): Vitrification of canine cumulusoocyte complexes in DAP213 with a cryotop holder. Reprod. Med. Biol. 9, 2: 115-120. Papers/Abstracts Published in Proceedings: 8. Mohammed A. Abdel-Ghani: Changes in blood flow, progesterone, estradiol-17β, nitric oxide after estrus synchronization during breeding season of sa'idi goats. The International Conference of Animal Production. Pp 25 (2015, April, El-Harghda, Egypt). 9. Mohammed A. Abdel-Ghani: Effects of treatment with GDF-9 and VEGF on the progression and survival of follicles in cryopreserved canine ovarian tissues in culture. The European Veterinary Conference "Voorjaarsdagen". (2014, April, Amsterdam, Netherland). 10. Mohammed A. Abdel-Ghani: Nuclear competence profile of canine oocytes with/ without direct contact between canine oocytes and bovine granulosa cell monolayers. The 24th Egyptian society conference for animal infertility. (2014, February, El-Harghda, Egypt). 11. Mohammed A. Abdel-Ghani: Effect of bovine cumulus–oocyte complexes conditioned medium on in vitro maturation of canine oocytes. The 45th Southern European Veterinary Conference. Pp 8 (2010, October, Barcelona, Spain). 12. Mohammed A. Abdel-Ghani: Influence of graft site and gonadotrophins on follicular development of canine ovarian grafts transplanted to NOD-SCID mice: inference for canine ovarian xenograft. The 45th Southern European Veterinary Conference. Pp 9 (2010, October, Barcelona, Spain). 13. Mohammed A. Abdel-Ghani: The effect of bovine and canine granulose monolayers on in vitro maturation of canine oocytes. The 150th Japanese Veterinary Meeting. Pp 263 (2010, September, Obihrio, Japan).