Supplementary Figure 1: Gene expression data obtained by RQ-PCR analysis. Graphics of 18
genes included in the set of predictor genes for APL (grey bars), AML with inv(16) (shaded bars)
and remaining AML (black bars) classes, as well as graphics of PBX3 and RUNX3 genes. Each
bar represents the log2 ratio between intensity value from each sample and from the average of
the three control samples.
Supplementary Figure 2: Gene expression patterns of the 11 genes that provide an optimal
class assignment to APL and non-APL classes. Each column represents a leukemia sample and
each row a gene. Red indicates a gene is expressed at a relatively high level while blue indicates
that a gene is expressed at a relatively low level. The upper 7 genes have relatively high
expression levels in APL samples and the lower 4 genes have relatively high expression levels in
non-APL cases. Color scale indicates relative expression in standard deviations from the mean.
Supplementary Figure 3: Graphics of expression levels of CBFB and MYH11 genes obtained by
RQ-PCR analysis. (1): gene fragment included in the CBFB-MYH11 fusion transcript. (2): gene
fragment not contained in the CBFB-MYH11 fusion transcript. Shaded bars correspond to AML
with inv(16) and black bars to AMLs without inv(16).
Supplementary Figure 4: Colorgram of genes distinguishing cluster A from cluster B in
remaining AML (22 samples not grouped into clusters according to karyotype or morphological
criteria). Each column represents a leukemia sample and each row a gene. Red color indicates
high level expression and blue, low level expression. Color scale indicates relative expression in
standard deviations from the mean. Cases marked with an asterisk correspond to
refractory/relapse patients.