PRESS OFFICE • 15 MetroTech Center, 6th Floor, Brooklyn, NY 11201
For Immediate Release:
Contact: Kathleen Hamilton
Thursday, October 23, 2014
718-260-3792/mobile 347-843-9782
kathleen.hamilton@nyu.edu
NYU RESEARCHERS BREAK NANO BARRIER
TO ENGINEER THE FIRST PROTEIN MICROFIBER
New Material Advances Tissue Engineering and Drug Delivery
Researchers at the New York University Polytechnic School of Engineering have broken new ground in the development of
proteins that form specialized fibers used in medicine and nanotechnology. For as long as scientists have been able to create
new proteins that are capable of self-assembling into fibers, their work has taken place on the nanoscale. For the first time,
this achievement has been realized on the microscale—a leap of magnitude in size that presents significant new opportunities
for using engineered protein fibers.
Jin Kim Montclare, an associate professor of chemical and biomolecular engineering at the NYU School of Engineering, led
a group of researchers who published the results of successful trials in the creation of engineered microfiber proteins in the
journal Biomacromolecules.
Many materials used in medicine and nanotechnology rely on proteins engineered to form fibers with specific properties. For
example, the scaffolds used in tissue engineering depend on engineered fibers, as do the nanowires used in biosensors. These
fibers can also be bound with small molecules of therapeutic compounds and used in drug delivery.
Montclare and her collaborators began their experiments with the intention of designing nanoscale proteins bound with the
cancer therapeutic curcumin. They successfully created a novel, self-assembling nanoscale protein, including a hydrophobic
pore capable of binding small molecules. To their surprise, after incubating the fibers with curcumin, the protein not only
continued to assemble, but did so to a degree that the fibers crossed the diameter barrier from the nanoscale to the microscale,
akin to the diameter of collagen or spider silk.
“This was a surprising and thrilling achievement,” said Montclare, explaining that this kind of diameter increase in the
presence of small molecules is unprecedented. “A microscale fiber that is capable of delivering a small molecule, whether it
be a therapeutic compound or other material, is a major step forward.”
Montclare explained that biomaterials embedded with small molecules could be used to construct dual-purpose scaffolds for
tissue engineering or to deliver certain drugs more efficiently, especially those that are less effective in an aqueous
environment. Using microscopy, the team was able to observe the fibers in three dimensions and to confirm that the
curcumin, which fluoresces when bound to structural protein, was distributed homogeneously throughout the fiber.
-more-
Despite the enormity of the jump from nano- to microscale, the research team believes they can devise even larger fibers. The
next step, Montclare says, is developing proteins that can assemble on the milliscale, creating fibers large enough to see with
the naked eye. “It’s even possible to imagine generating hair out of self-assembly,” she says.
Researchers from three institutions collaborated on this work. In addition to Montclare, NYU School of Engineering doctoral
candidate Jasmin Hume, graduate student Rudy Jacquet, and undergraduate student Jennifer Sun co-authored the paper.
Richard Bonneau, an associate professor in NYU's Department of Biology and a member of the computer science faculty at
NYU's Courant Institute of Mathematical Sciences, and postdoctoral scholar P. Douglas Renfrew also contributed, along with
M. Lane Gilchrist, associate professor of chemical engineering at City College of New York and master’s degree student
Jesse A. Martin, also from City College. Their work was supported by the Army Research Office and the National Science
Foundation.
The full study, “Engineered Coiled-Coil Protein Microfibers,” is available at
http://pubs.acs.org/doi/pdf/10.1021/bm5004948).
The NYU Polytechnic School of Engineering dates to 1854, when the NYU School of Civil Engineering and
Architecture as well as the Brooklyn Collegiate and Polytechnic Institute (widely known as Brooklyn Poly) were
founded. Their successor institutions merged in January 2014 to create a comprehensive school of education and
research in engineering and applied sciences, rooted in a tradition of invention, innovation and entrepreneurship.
In addition to programs at its main campus in downtown Brooklyn, it is closely connected to engineering
programs in NYU Abu Dhabi and NYU Shanghai, and it operates business incubators in downtown Manhattan
and Brooklyn. For more information, visit http://engineering.nyu.edu.
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