George Mason University – Graduate Council
Graduate Course Approval Form
All courses numbered 500 or above must be submitted to the Graduate Council for final approval after approval by the
sponsoring College, School or Institute.
Graduate Council requires submission of this form for a new course or any change to existing courses. For a new course,
please attach a copy of the syllabus and catalog description (with catalog credit format, e.g. 3:2:1). The designated
representative of the College, School or Institute should forward the form along with the syllabus and catalog description, if
required, as an email attachment (in one file) to the secretary of the Graduate Council. A printed copy of the form with
signatures and the attachments should be brought to the Graduate Council meeting. Please complete the Graduate Course
Coordinator Form if the proposed changes will affect other units.
Note: Colleges, Schools or Institutes are responsible for submitting new or modified catalog descriptions (35 words or
less, using catalog format) to Creative Services by deadlines outlined in the yearly Catalog production calendar.
Please indicate: New_______
Modify___X____
Delete_______
Department/Unit:___MMB___________ Course Subject/Number:__BIOL 585____________________________
Submitted by:____Paulette W. Royt___________ Ext:___31058________ Email:_____proyt@gmu.edu____
Course Title
Eukaryotic Cell Biology Laboratory____________________________________________
Effective Term (New/Modified Courses only): ___Spring 08__
Credit Hours: (Fixed) __2___
(Var.) _____ to ____
Final Term (deleted courses only):____________
Grade Type (check one):
__X___ Regular graduate (A, B, C, etc.)
_____ Satisfactory/No Credit only
_____ Special graduate (A, B, C, etc. + IP)
Repeat Status*(check one): ___ NR-Not repeatable __X__ RD-Repeatable within degree ____ RT-Repeatable within term
*Note: Used only for special topics, independent study, or internships courses
Total Number of Hours Allowed: ___4__
Schedule Type Code(s): 1.___X_ LEC=Lecture SEM=Seminar STU=Studio INT=Internship IND=Independent Study
2.___X_ LAB=Lab RCT=Recitation (second code used only for courses with Lab or Rct component)
Prereq _X__ Coreq ___ (Check one):__BIOL 484 or BIOL 682_or POI____________________________
__________________________________________________________________________________________
Note: Modified courses - review prereq or coreq for necessary changes; Deleted courses - review other courses to correct prereqs that list the deleted course.
Description of Modification (for modified courses):____Change prerequisites, and credits from 1 to 1-2.
Special Instructions (major/college/class code restrictions, if needed):______A catalogue description and new syllabus
are attached.
Department/Unit Approval Signature:______Paulette W. Royt_____________ Date: 11/13/06_____________
College/School Committee Approval Signature:__________________________________ Date:_____________
Graduate Council Approval Date:____________ Provost Office Signature:_________________________________
Catalogue Description
585 Eukaryotic Cell Biology Laboratory (1-2:0-1:3) Prerequisites:BIOL 484 or BIOL 682, or
permission of instructor. Selected topics of laboratory procedures used in the study of
eukaryotic cells. May be repeated one time with permission of program director.
George Mason University
Graduate Course Coordination Form
NA
Approval from other units:
Please list those units outside of your own who may be affected by this new, modified, or deleted course. Each of these units must
approve this change prior to its being submitted to the Graduate Council for approval.
Unit:
Head of Unit’s Signature:
Date:
Unit:
Head of Unit’s Signature:
Date:
Unit:
Head of Unit’s Signature:
Date:
Unit:
Head of Unit’s Signature:
Date:
Unit:
Head of Units Signature:
Date:
Graduate Council approval: ______________________________________________ Date: ____________
Graduate Council representative: __________________________________________
Date: ____________
Provost Office representative: ____________________________________________
Date: ____________
SAMPLE SYLLABUS
BIOL 585 Eukaryotic Cell Biology Laboratory
Instructor: Dr. Geraldine Grant,
Email: ggrant1@gmu.edu
Phone: 703-993-4292
Office Hours: By appointment. Discovery Hall 207
Class Time: Spring term. Weekly lectures to spring break. Spring break Monday to Friday 9am – 5pm BRH
Rm 363.
Prerequisites: Biol 484 or 682 or equivalent, or permission of instructor. This 2 credit
lecture/ laboratory course is designed to introduce the student to many of the techniques essential to the study of
eukaryotic cell biology through tissue/cell culture techniques. The student will be introduced to primary tissue
culture and tissue culture applications such as toxicity testing, and gene delivery. Students will also be
introduced to histological, immunological, and fluorescent microscopy and forensic PCR.
Course Requirements: Students are required to attend lectures prior to spring break, and to attend laboratory
sessions each day of spring break. Students will complete each of the tasks as designed keeping an accurate
notebook of the week’s experiments and results. Students will be graded on their laboratory notebook, results
presentation, and a final exam given one week post the course completion.
Homework: Student are required to keep and accurate daily notebook and prepare a complete report of the
week’s work to be completed and submitted one week post the completion of the course. Students must come
prepared for class.
BIOL 585 Grading
Final Exam one week post course completion
Laboratory Note Book and report
50%
50%
Grade Scale: 96-100 = A+, 93-95 = A, 90-92=A-, 87-89=B+, 83-86=B, 80-82=B-, 77-79=C+, 70-76=C, 69 or
below=F.
Attendance Policy: Attendance is required.
Class Website: http://mason.gmu.edu/~ggrant1
Honor Code: The Honor Code will be in force for this and all classes at GMU.
Text:
Culture of Animal Cells – A manual of basic techniques. R. Ian Freshney 4th edition (3rd is fine also) WileyLiss. Publication. ISBN 0-471-34889
Library Location: Fenwick Stacks Call Number: QH585.2 .F74 2000
Additional Reading:
Cell and Tissue Culture: Laboratory Procedures in Biotechnology. Alan Doyle and J. Bryan Griffiths. Wiley
Publication. ISBN 0-471-98255-5
Animal Cell Culture Methods. Jennie P. Mather and David Barnes. Academic Press. ISBN 0-12-544159-2 (hp)
TECHNIQUES
1.
General cell culture techniques
a. Media preparation
b. Sterility confirmation
c. Trypsinization of cells
d. Cell Counting
e. Viability testing of cells
f. Freezing of cells
g. Thawing of cells
2.
Transfection of rat H4IIE cells with reporter plasmid
a. Transfection – Lipofectamine V electroporation
b. Efficiency of transfection.
3.
Cyto-toxicity testing
a. Prep of miniaturized assay/ 96 well assay
b. Dilution series
c. Addition of drug
d. Determination of cell survival
e. Analysis of results
4.
Primary cell culture
a. Culture of mouse kidney cells
b. Histochemistry H&E staining
c. Immunohistochemistry
5.
Cross contamination detection using PCR
6.
Chromosomal analysis – G banding