Table A. Thirteen previously reported mouse genes that are expressed in
spermatogonia but not in somatic tissues
Gene
Mage
Ube1y
Usp9y
Rbmy
Tuba3/Tuba7
Stra8
Ott
Sycp2
Sycp1
Figla
Sycp3
Ddx4
Dazl
Expression
Testis
Testis
Testis
Testis
Testis
Testis
Testis and ovary
Testis and ovary
Testis and ovary
Testis and ovary
Testis and ovary
Testis and ovary
Testis and ovary
Chromosome
X
Y
Y
Y
6
6
X
2
3
6
10
13
17
References
31
32
33
8
34
35
36
37
38
39
40
41
6,7
Supplementary references and notes
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33. Brown, G.M. et al. Characterisation of the coding sequence and fine mapping of the
human DFFRY gene and comparative expression analysis and mapping to the Sxrb
interval of the mouse Y chromosome of the Dffry gene. Hum. Mol. Genet. 7, 97-107
(1998).
34. Villasante, A. et al. Six mouse alpha-tubulin mRNAs encode five distinct isotypes:
testis-specific expression of two sister genes. Mol. Cell. Biol. 6, 2409-2419 (1986).
35. Oulad-Abdelghani, M. et al. Characterization of a premeiotic germ cell-specific
cytoplasmic protein encoded by Stra8, a novel retinoic acid-responsive gene. J. Cell
Biol. 135, 469-477 (1996).
36. Kerr, S.M., Taggart, M.H., Lee, M. & Cooke, H.J. Ott, a mouse X-linked multigene
family expressed specifically during meiosis. Hum. Mol. Genet. 5, 1139-1148 (1996).
37. Offenberg, H.H. et al. SCP2: a major protein component of the axial elements of
synaptonemal complexes of the rat. Nucleic Acids Res. 26, 2572-2579 (1998).
38. Sage, J. et al. The Sycp1 loci of the mouse genome: successive retropositions of a
meiotic gene during the recent evolution of the genus. Genomics 44, 118-126 (1997).
39. Liang, L., Soyal, S.M. & Dean, J. FIGalpha, a germ cell specific transcription factor
involved in the coordinate expression of the zona pellucida genes. Development 124,
4939-4947 (1997).
40. Klink, A., Lee, M. & Cooke, H.J. The mouse synaptosomal complex protein gene
Sycp3 maps to band C of chromosome 10. Mamm. Genome 8, 376-377 (1997).
41. Fujiwara, Y. et al. Isolation of a DEAD-family protein gene that encodes a murine
homolog of Drosophila vasa and its specific expression in germ cell lineage. Proc.
Natl. Acad. Sci. USA 91, 12258-12262 (1994).
42. We have also identified putative X-linked human orthologs of the other three novel
X-linked mouse genes: Tktl1, Tex16, and Pramel3. The human TKTL1 gene was
previously discovered and shown to be expressed in many somatic tissues [J. F. Coy
et al., Genomics 32, 309 (1996)]; we found that the gene is abundantly transcribed in
testes (not shown). We identified human X-linked sequences (Genbank Z95126)
homologous to mouse Tex16 but observed no expression (as judged by RT-PCR) in
any of the eight human tissues listed in Fig. 3; the human ortholog may be a
pseudogene. We identified human X-linked sequences (Genbank Z70226)
homologous to mousePramel3 but have not characterized the human gene's
expression or cDNA sequence. The search for human orthologs of the remaining
seven novel autosomal mouse genes (Pramel1, Tex17, Rnh2, Tex18, Piwil2, Tex20,
Tex19) is complicated by the existence of multiple related sequences in the human
genome and/or the inavailability of corresponding human genomic sequence. .
43. Lawson, D.M. et al. Solving the structure of human H ferritin by genetically
engineering intermolecular crystal contacts. Nature 349, 541-544 (1991).
44. Baker, R.T., Tobias, J.W. & Varshavsky, A. Ubiquitin-specific proteases of
Saccharomyces cerevisiae. Cloning of UBP2 and UBP3, and functional analysis of
the UBP gene family. J. Biol. Chem. 267, 23364-23375 (1992).
45. Coy, J.F. et al. Molecular cloning of tissue-specific transcripts of a transketolaserelated gene: implications for the evolution of new vertebrate genes. Genomics 32,
309-316 (1996).
46. Chiang, C.M. & Roeder, R.G. Cloning of an intrinsic human TFIID subunit that
interacts with multiple transcriptional activators. Science 267, 531-536 (1995).
47. van Baren, N. et al. PRAME, a gene encoding an antigen recognized on a human
melanoma by cytolytic T cells, is expressed in acute leukaemia cells. Br. J. Haematol.
102, 1376-1379 (1998).
48. Segref, A. et al. Mex67p, a novel factor for nuclear mRNA export, binds to both
poly(A)+ RNA and nuclear pores. Embo J. 16, 3256-3271 (1997).
49. Gruter, P. et al. TAP, the human homolog of Mex67p, mediates CTE-dependent RNA
export from the nucleus. Mol. Cell 1, 649-659 (1998).
50. Kang, Y. & Cullen, B.R. The human Tap protein is a nuclear mRNA export factor
that contains novel RNA-binding and nucleocytoplasmic transport sequences. Genes
Dev. 13, 1126-1139 (1999).
51. Herold, A. et al. TAP (NXF1) belongs to a multigene family of putative RNA export
factors with a conserved modular architecture. Mol. Cell. Biol. 20, 8996-9008. (2000).
52. Hanks, S.K. & Quinn, A.M. Protein kinase catalytic domain sequence database:
identification of conserved features of primary structure and classification of family
members. Methods Enzymol. 200, 38-62 (1991).
53. Ponting, C.P. Tudor domains in proteins that interact with RNA. Trends Biochem.
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54. Kobe, B. & Deisenhofer, J. The leucine-rich repeat: a versatile binding motif. Trends
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55. Mouse Rnf17 appears to encode a protein of 626 residues. A mouse cDNA sequence
corresponding to the 5' portion of Rnf17 was reported recently; it appeared to encode
a protein of 316 residues [X. Y. Yin, K. Gupta, W. P. Han, E. S. Levitan, E. V.
Prochownik, Oncogene 18, 6621 (1999)]. The discrepancy may be the result of
alternative splicing or sequencing errors near the 3' end of the previously reported
cDNA sequence (compare GenBank AF190166 [1098 nucleotides; 951 nucleotide
open reading frame] with GenBank AF285585 [2094 nucleotides; 1881 nucleotide
open reading frame]). Yin and colleagues demonstrated that the portion of the protein
encoded by their partial cDNA interacted with mad proteins in vitro. In the case of
human RNF17, alternative splicing appears to generate two protein isoforms. .
56. Cox, D.N. et al. A novel class of evolutionarily conserved genes defined by piwi are
essential for stem cell self-renewal. Genes Dev. 12, 3715-3727 (1998).
57. Schmidt, A. et al. Genetic and molecular characterization of sting, a gene involved in
crystal formation and meiotic drive in the male germ line of Drosophila
melanogaster. Genetics 151, 749-760 (1999).
58. Mooslehner, K., Muller, U., Karls, U., Hamann, L. & Harbers, K. Structure and
expression of a gene encoding a putative GTP-binding protein identified by provirus
integration in a transgenic mouse strain. Mol. Cell. Biol. 11, 886-893 (1991).