DANNIELLE A. RYMAN
248 Amherst Rd Apt D4
Sunderland, MA 01375
Phone (302)359-9035
dannielle.ryman@gmail.com
dryman@mcb.umass.edu
UMass ID 25759014
EDUCATION
Spring 2008-Summer 2009
Delaware State University, Dover, DE
Biotechnology Major
Courses completed in Cell Biology, Genetics, Molecular Biology, Forensic Biology, Protein
Biotechnology, Nucleic Acid Biotechnology, Graduate Molecular Biology, Organic Chemistry I &
II and Biochemistry
2007-2009
Dean’s List, Delaware State University, Cum Laude
2003-2007
Delaware Technical and Community College, Georgetown, Delaware
Biotechnology Major
Courses completed in Biology I & II, Microbiology, and Biotechnology
2004-2007
Dean’s List, Delaware Technical and Community College
2004-2005
Alpha Beta Gamma Honor Society, Delaware Technical and Community College
RESEARCH EXPERIENCE
July 2009-July2010
Research Technician
Contract by Franklin Co. Position held at Pioneer, A DuPont Company, Wilmington, DE
Robert Williams
Prepare and evaluate Arabidopsis plants. Recordkeeping. Plant husbandry. Seed collection. Cold
shock. Transformation. MiniPrep. Infiltration. LemnaTec imaging. Media prep. DNA extraction.
PCR. Sequencing. Cloning. Drought and Hydrotropism assay.
January 2008-July 2009
Research Assistant
Dr. Vincent Fondong, Associate Professor,
Delaware State University
Plant tissue culture. Media preparation, large scale culture preparation. Plant husbandry.
Molecular cloning. DNA and RNA extraction. Recordkeeping. Accounting. Ordering of lab
equipment and reagents. Supervising and assisting graduate and undergraduate student
researchers. Transformation of E. coli with vaccine protein genes. Mini prep of plasmid DNA.
PCR amplification. DNA recovery from agarose gel using QIAGEN DNA Recovery Kit.
Recombination reaction using BP Clonase (construction of entry vectors). Mini prep of entry
vector. Gateway Reactions. Mini prep of expression vectors. Agrobacterium transformation.
Agrobacterium infiltration of Nicotiana benthiamana plant tissue using expression vectors. Gel
Electrophoresis. Protein extraction for Western blot analysis. SDS-PAGE. Western hybridization.
Affinity chromatography (protein purification). Enzyme Linked Immuno-Sorbent Assay (ELISA)
of purified protein aliquots to quantify protein concentration . Bacterial cell culture techniques
(preparation of mid-log cell cultures to prepare a competent cell culture). Restriction analysis.
Rapid colony transformation of E. coli. Classical transformation of E. coli. Mini prep.
Recombination of antibiotic resistance genes. Transformation using recombinant antibiotic
resistance plasmids and replica plating for identification. Purification & identification of
recombinant plasmid DNA. Recombination of PCR product. Southern Hybridization. VNTR
analysis (DNA fingerprinting) using CODIS primers and PCR. DNA methylation analysis
(epigenetics).
June-August 2007
Delaware State University REU (Research Experience for Undergraduates) Internship
Role of three proteins encoded in EACMV (East African Cassava Mosaic Virus) on the replication and
spread of ACMV (African Cassava Mosaic Virus
Cloning genes in binary vectors. DNA Extraction. Electrophoresis. Agar preparation. Classical
and rapid colony transformation of E coli. Miniprep. Recombination. Agro bacterium infiltration.
Southern hybridization. Protein extraction. Western blot.
PUBLICATIONS
2009
Characterization of the cassava geminivirus transcription activation protein putative nuclear
localization signal, Chowda-Reddy RV, Dong W, Felton C, Ryman D, Ballard K, Fondong VN.
Department of Biological Sciences, Delaware State University, 1200 North DuPont Highway,
Dover, DE 19901, United States. Virus Research. 2009, Aug 6.