SYLLABUS
CELL BIOLOGY
INSTITUTE OF APPLIED BIOTECHNOLOGY
AND BASIC SCIENCES
COURSE TITLE
FACULTY/INSTITUTE
COURSE CODE
DEGREE PROGRAMME
FIELD OF STUDY
DEGREE LEVEL
FORMA STUDIÓW/STUDY
MODE
BS
LABORATORY
SECOND YEAR, SUMMER SEMESTER
WNUK MACIEJ
COURSE OBJECTIVES
The successful student in this course will develop a deeper understanding of what life is and how it
functions at the cellular level. Students will develop skills in interpreting scientific data, designing
and executing cellular experiments, and communicating scientific data and concepts clearly and
effectively. develop a basic knowledge and understanding of the major integrating concepts of the
biological sciences (chemical basis for life, cell theory, inheritance, anatomy and physiology);
develop knowledge of the structure and function of the cell.
COURSE FORMAT
YEAR AND SEMESTER
NAME OF THE TEACHER
PREREQUISITES
LEARNING OUTCOMES
Students are expected to possess a basic proficiency in general
chemistry, organic chemistry, and biology. It is the student’s
responsibility to review background materials as necessary
KNOWLEDGE:
Understand and utilize the scientific vocabulary used in
communicating information in cell and molecular biology
• Understand and apply general concepts of cell and molecular
biology to relevant, specific problems
• Describe and discuss the properties and biological significance of
the major classes of molecules found in living organisms and the
relationship between molecular structure and biological function
• Represent and illustrate the structural organization of genes and
the control of gene expression
• Conceptualize and describe protein structure, folding and sorting
• Explain the structure of membranes and intracellular
compartments and relate these to function.
• Summarize the processes of energy transduction in cells
• Relate how cell movement and cell-cell communication occur and
discuss mechanisms of signal transduction
• Outline the processes that control eukaryotic cell cycle and cell
death.
• Link the rapid advances in cell and molecular biology to a better
understanding of diseases, including cancer.
SKILLS:
Basic skills in pipetting, liquid handling, balances, pH meter,
preparation of buffers, Sterile technique for cell culture, Cell culture
and cryostorage, Isolation of plasmid DNA from E. coli, Monitoring
enzymatic digestion of yeast cell wall, Cell transformation by
introduction of plasmid DNA, Light and fluorescence microscopy:
Detection of GFP-fusion proteins in cells
Cell fractionation: cell disruption and organelle
separationPreparation of soluble cell extracts and detergentsolubilized cell extracts, Affinity purification of proteins from cell
extracts, Detection of protein
-protein interactions, Determination of protein concentra
tion by Bradford assay, Column chromatography
-desalting columns, SDS PAGE analysis of proteins
Western blot detection of proteins
COURSE ORGANISATION –LEARNING FORMAT AND NUMBER OF HOURS
15 HRS LECTURE, 30 HRS - LABORATAORY
COURSE DESCRIPTION
LECTURE – 15 hrs
 MOLECULAR GENETICS, GENES, AND CHROMOSOMES: Nucleic Acid Structure,
Transcription, and Decoding, Chromosomal Organization of Eukaryotic Genes, Chromatin
Structure -3hrs
 CONTROL OF GENE EXPRESSION: Eukaryotic and Prokaryotic Transcription Control
Elements and RNA Polymerases Cytoplasmic Posttranscriptional Control; RNA Interference;
mRNA Degradation – 3hrs

CELLULAR FUNCTIONS: Membranes and Membrane Transport, Protein Targeting and
Transport, Cellular Energetics, Secretion and Endocytosis, Cell Signaling 3 hrs

CYTOSKELETON: Cytoskeleton: Actin and Myosin, Microtubules, Intermediate Filaments,
Cell Adhesion, & Extracellular Matrix 2 hrs

CELL GROWTH: Cell Cycle, Stem Cells and Cell Differentiation, Regulation of Cell Death
and Cancer, Cancer: Tumor Cells, Genetic Basis, Senesence and Aging 4hrs
LABORATORY -30Hrs
 In vitro cell culture: Cell morphology, general procedure of cell culture- 3 hrs
 Preparation of Metaphase Spread from in vitro cell culture- 3hrs
 Detection centromeric sequences by Fluorescence in situ Hybridization – 6 hrs
 Apoptosis – Cell death tests: TUNEL assay, AO/ETBR, cell cultures for in vitro assays – 6
hrs
 Cell Cycle – p53 and p21 detection by immunofluorescence – 6 hrs
 Fluorimetric ROS assay, In vitro Thiol content determination – 3 hrs
 Cell Senescence – morphology and beta gal test- 3hrs.
POWERPOINT PRESENTATION (LECTURE), Practical
Course (Laboratory)
AND Lecture –Oral presentation from Lecture subject
(presentation should be limited to 60 minutes)- examination
Laboratory- on the basis Research paper analysis and
Written reports
After the completion of an experiment, each student should
prepare a journal style article for the lab report.This should
include:
•Abstract: concise summary of what happened during the
METHODS OF INSTRUCTION
REQUIREMENTS
ASSESSMENTS
GRADING SYSTEM
TOTAL STUDENT WORKLOAD
NEEDED TO ACHIEVE
EXPECTED LEARNING
OUTCOMES EXPRESSED
IN TIME AND ECTS CREDIT
POINTS
LANGUAGE OF INSTRUCTION
INTERNSHIP
MATERIALS
experiment (2-3 sentences for each experiment)
•Introduction: to provide adequate b
ackground pertaining to the cell line and experiments to
give anyreader knowledge of why you did the experiment.
This should include your hypothesis.
•Materials and Methods: concise summary of what you did
including how the cells were prepared and
maintained.
•Results: This section will include both figures and written
results. Here you state simply what you saw.
•Discussion: This section you will analyze your results and
state why you observed what you did during
the experiment. (Conclusions section)
•References: you will need to be looking for references to
support your introduction and discussion.
2-5
110 HRS/4 ECTS
ENGLISH
PRIMARY OR REQUIRED BOOKS/READINGS:
Molecular biology of the cell, Fifth edition.
(2008) Bruce Alberts, Alexander Johnson,
Julian Lewis, Martin Raff, Keith Roberts, and Peter Walter.
Garland Science Publishers, New York, NY.
ISBN: 978-0-8153-4105-5.
SUPPLEMENTAL OR OPTIONAL
BOOKS/READINGS:
PUBMED- DIFFERENT SCIENTIFIC PAPERS
LABORATORY AND SAFETY CONDUCT:
FAILURE TO COMPLY WITH SAFETY CONDUCT WILL RESULT IN
IMMEDIATE EXPULSION FROM LAB FOR THE DAY AND A ZERO
WILL BE GIVEN FOR THE DAY’S ASSIGNMENTS.
1.FOOD AND DRINKS ARE NOT ALLOWED IN THE LAB
2.CLOSED TOED SHOES THAT COMPLETELY COVER THE FOOT MUST BE WORN AT ALL TIMES
IN LAB. (BALLET FLATS OR SIMILAR SHOES THE EXPOSE THE TOP OF THE FOOT ARE NOT
ALLOWED)
3.LONG JEANS OR PANTS MUST BE WORN. SHORTS AND CAPRIS ARE NOT ALLOWED.
4.LONG HAIR MUST BE TIED BACK AND KEPT AWAY FROM FLAME
5.LATEX OR VINYL GLOVES MUST BE WORN WHILE CONDUCTING EXPERIMENTS OR
HANDLING REAGENTS ORORGANISMS
6.SPILLS OR BROKEN GLASS MUST BE REPORTED IMMEDIATELY
7.BIOLOGICAL WASTES MUST BE DISPOSED OF CORRECTLY. (MICROPIPETTE TIPS IN THE RED
BOXES, PIPETTE TIPS IN THE CREAM CYLINDERS, CULTURE DISHES, TEST TUBES, ETC. IN THE
BIOLOGICAL WASTE/GLASS CONTAINER BOXES)
8.AVOID BREATHING IN OR TASTING CHEMICALS–NEVER PIPETTE BY MOUTH
9.NO UNAUTHORIZED EXPERIMENTS ARE ALLOWED
10.CULTURE TUBES ARE TO BE PLACED IN A RACK, NEVER ON THE COUNTER
11.CELL PHONES SHOULD BE PLACED ON SILENT OR BE TURNED OFF
12.DISRUPTIVE BEHAVIOR WILL RESULT IN EXPULSION FROM LAB
13.REMEMBER TO USE CAUTION WHEN HANDING GLASSWARE OR SHARP TOOLS.
14.AT THE START OF EACH LAB THE BENCH TOP MUST BE WIPED OFF WITH DISINFECTANT.