Systems-level analysis of host-pathogen interactions using mass

Systems-level analysis of host-pathogen interactions using mass spectrometry-based proteomics
(Times New Roman, 12, bold)
Xiaoyun Liu1, Beile Gao2, Jorge Galan2
Peking University, College of Chemistry, Beijing, 100871, China
Yale University, Department of Microbial Pathogenesis, New Haven, CT, 06519
Corresponding to:
Over the years characterization of individual virulence factors has been the mainstream in the
field of bacterial pathogenesis. However, a deeper and overall understanding of host-pathogen
interactions requires systems-level studies of dynamic microbial infection from an integrative
perspective. Emerging high-throughput technologies such as mass spectrometry-based proteomics
can be an ideal toolbox because it enables global quantitative measurements of protein constituents
in an organism as well as their post-translational modifications. Here the state-of-the-art proteomic
tools were utilized to examine the landscape of bacterial protein expression during infection with
Campylobacter jejuni as a model. Proteome-wide analysis indicated that this bacterium undergoes a
significant metabolic downshift including both aerobic and anaerobic respirations. Furthermore, our
results suggested that intracellular C. jejuni favors anaerobic respiration using fumarate as the
primary electron acceptors. These data are consistent with the observation that C. jejuni does not
replicate within cultured mammalian cells and provide an explanation to its poor ability to grow
under standard laboratory conditions when harvested from infected cells. (Times New Roman, 12)
1. Liu X, Valentine SJ, Plasencia MD, Trimpin S, Naylor S, Clemmer DE. J. Am. Soc. Mass Spectrom., 2007, 18(7),
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