“Detection and quantification of polyphenol

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SHORT CURRICULUM VITAE
Styliani Christophoridou, Msc., PhD.
PERSONAL DATA
Date of birth:
Place of birth:
Home Address:
Home Telephone:
Mobile phone:
Web:
e-mail:
12 February 1972
Thessaloniki, Greece
Seferi 72, 41221, Larisa, Greece.
+30 2410 285684
+30 6932158628
http://www.teilar.gr/person_en.php?pid=350
stellax@chemistry.uoc.gr
EDUCATION

2006 June: Post doctoral fellow, Chemistry Dept., University of
Crete, Heraklion Crete, Greece.
“Extraction and processing of marine aerosol samples in order to
obtain NMR spectra, application of NMR spectroscopy”

2005 March: Ph.D. in Physical Chemistry, Chemistry Dept.,
University of Crete, Heraklion Crete, Greece.
“Development and application of new analytical techniques based on
NMR spectroscopy for the detection and quantification of
polyphenolic compounds in virgin olive oil.”

2001 January: M.Sc. in Applied Molecular Spectroscopy,
Chemistry Dept., University of Crete, Heraklion Crete, Greece.
“Detection and quantification of polyphenol-containing olive oil
model compounds by employing 31P NMR spectroscopy”.

1998 September: BS in Chemistry, Chemistry Dept., University of
Crete, Heraklion Crete, Greece.

1997 September: Diploma Thesis in Physical Chemistry,
Chemistry Dept., University of Crete, Heraklion Crete, Greece.
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“A study of the overall motion of poly-α-(13)-D-glucopyranose and
poly-β-(13)-D-glucopyranose in DMSO solution by viscosity
measurements”.
RESEARCH INTERESTS

Plant Chemistry

Development and application of new analytical techniques in
quality control and plant analysis.

Plant antioxidants – Polyphenols.

Fats and oils.
PROFESSIONAL EXPERIENCE

September 2010 – … :
Scientific Collaborator (on contract)
Technological Educational Institute of Larissa, Department of
Nutrition and Dietetics, Karditsa, Greece, Coarse Tought: Food
Analysis,

September 2008 – … :
Scientific Collaborator (on contract)
Technological Educational Institute of Larissa, Department of Food
Technology, Karditsa, Greece

September 2007 – 2010:
Associate Lecturer (on contract)
Department of Biochemistry and Biotechnology, University of
Thessaly, Greece.

November 2006:
Research Fellow
Chemistry Department, University of Crete, Heraklion Crete,
Greece.

January 2006 – June 2006:
Post Doctoral Researcher
Chemistry Department, University of Crete, Heraklion Crete,
Greece.

September 2005 – June 2006:
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Laboratory Assistant (on contract)
Technological Educational Institute of Crete, Department of natural
resources and environment, Chania, Crete, Greece.

June 2003 – March 2004:
Chemistry Researcher
Chemistry Department, University of Crete, Heraklion Crete,
Greece.

September 2000 – June 2001:
Chemistry Researcher
Chemistry Department, University of Crete, Heraklion Crete,
Greece.
PARTICIPATION IN RESEARCH PROGRAMS

November
2006:
Chemistry
Researcher,
Research
Program
“Conformational analysis of mycotoxins by NMR spectroscopy and
computational molecular modeling”. Research Program cofunded by
the European Social Fund (75%) and National Sources (25% EPEAEK).

January 2006 –June 2006: Post Doctoral Researcher, Research
Program “Marine Aerosol Production: Primary & Secondary Marine
Aerosol Production from Natural Sources”. Research Program funded
by the European Union.

June
2003
–
March
2004:
Researcher,
Research
Program
“Identification of variety and age of olive oil from variety “Koroneiki”
and study of its antioxidant properties with respect to other main
Greek varieties”. Research Program funded by Minerva.

July 2003 – September 2003: Researcher, Research Program
“Designing and construction of analytical instruments”. Research
Program funded by Research committee, University of Crete, Crete,
Greece.

September 2000- June 2001: Researcher, Research Program “Novel
polymeric materials vinylpyrolidone copolymers with immunoactive
action”, Research Program funded by General Secretariat for Research
and Technology.
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TECHNICAL EXPERIENCE
Experience in the use of NMR spectrometers (Bruker AMX-500,
MSL-300), and chromatographic techniques (HPLC, Column
chromatography, preparative TLC), UV-VIS spectrometry, 2D gel
electrophoresis, Mass spectroscopy, Atomic Absorption, IR, RAMAN,
LASER spectroscopy.
Experience in the use of Office, AMIX, WINNMR, Mestrec,
Statistica, SPSS, Mathematica, CS ChemOffice, Origin, etc.
PUBLICATIONS
1. “31P Nuclear Magnetic Resonance spectroscopy of polyphenolcontaining olive oil model compounds”. S. Christophoridou, A. Spyros
and Photis Dais, Phosphorus Sulfur and Silicon, 2001, 170, 139.
2. “Determination of the diglycerides content in Greek virgin olive
oils and some commercial olive oils by employing
31P
NMR
spectroscopy”. P. Fronimaki, A. Spyros, S. Christophoridou and P.
Dais, J. Agric. Food Chem., 2002, 50, 2207.
3. “Separation and Identification of Phenolic Compounds in Olive
Oil by Coupling High Performance Liquid Chromatography with Postcolumn Solid Phase Extraction to Nuclear Magnetic Resonance
Spectroscopy (LC-SPE- NMR).” S. Christophoridou, P. Dais, Li-Hong.
Tseng, and M. Spraul, J. Agric. Food Chem., 2005, 53, 4667-4679.
4. “Novel Approach to the Detection and Quantification of Phenolic
Compounds in Olive Oil Based on
31P
Nuclear Magnetic Resonance
spectroscopy.” S. Christophoridou, P. Dais, J. Agric. Food Chem.,
2006, 54, 656-664.
5. “Comparison of quantitative NMR spectroscopy with
conventional methods of analysis for the determination of olive oil
constituents” Photis Dais, Apostolos Spyros, Emmanuel Hatzakis,
Stella Christophoridou, Georgia Fragaki, Alexia Agiomyrgianaki,
Emmanuel Salivaras, George Siragakis, Despina Daskalaki, Maria
Tassioula-Margari, and Manuel Brenes, J. Agric. Food Chem., 2007,
55, 577-584.
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6. “Geographical Characterization of Greek Virgin Olive Oils (Cv.
Koroneiki) Using 1H and 31P NMR Fingerprinting with Canonical
Discriminant Analysis and Classification Binary Trees”, Panos V.
Petrakis, Alexia Agiomyrgianaki, Stella Christophoridou, Apostolos
Spyros, and Photis Dais, J. Agric. Food Chem., 2008, 56, 3200-3207.
7. “Detection and Quantification of Phenolic Compounds in Olive
Oil by Employing High Resolution 1H NMR Spectroscopy. A proposal
for on-line olive oil samples screening.” S. Christophoridou, and P.
Dais, Analytica Chimica Acta, 633 (2), Feb 2009, p.283-292.
PARTICIPATION IN CONFERENCES
1. P. Dais, S. Christophoridou and A. Spyros “Detection and
quantification of polyphenol-containing olive oil model compounds by
employing
31P
NMR spectroscopy”: 5th International Conference on
Applications of Magnetic Resonance in Food Science, September 1820, 2000, Aveiro, Portugal.
2. P. Dais, S. Christophoridou, A. Spyros, P. Fronimaki
“Diglycerides, a useful index for the quality control of virgin olive oil”:
18th Panhellenic Chemistry Conference, March 10-13, 2001, Piraeus,
Greece.
3. A. Spyros, P. Fronimaki, S. Christophoridou, P. Dais “Vegetable
oils characterization and classification via chemometrics and NMR
Spectroscopy. ”:18th Panhellenic Chemistry Conference, March 10-13,
2001, Piraeus,Greece.
4. S. Christophoridou, A. Spyros and P. Dais “1D and 2D NMR
spectroscopy of model polyphenols. A first approach for the
determination of polyphenols in virgin olive oil”. 18th Panhellenic
Chemistry Conference, March 15-17, 2001, Piraeus, Greece.
5. S. Christophoridou and P. Dais “Determination of free and
esterified hydroxytyrosol and tyrosol in virgin olive oil by employing
31P-NMR
spectroscopy. Indices of oxidative stability of olive oil. “:19th
Panhellenic Chemistry Conference, November 6-10, 2002, Heraklion,
Greece.
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6. P. Dais, P. Fronimaki, S. Christophoridou, A. Spyros “A new
methodology for the determination of virgin olive oil quality and
authenticity”: 19th Panhellenic Chemistry Conference, November 6-10,
2002, Heraklion, Greece.
7. Photis Dais, S. Christophoridou “A Novel Approach for Detection
and Quantification of Phenolic Compounds in Olive Oil Based on
31P
NMR Spectroscopy.” 3rd Euro Fed Lipid Congress, September 5-8,
2004, Edinburgh, Scotland.
8. Photis Dais, S. Christophoridou, Li-Hong Tseng, M. Spraul
“Identification of Phenolic Compounds in Olive Oil by Coupling Liquid
Chromatography with Nuclear Magnetic Resonance Spectroscopy (LC
NMR).” The 7th International Conference on Applications of Magnetic
Resonance in Food Science, September 13-15, 2004, Copenhagen,
Denmark.
9. Photis Dais, S. Christophoridou “A Novel Approach for Detection
and Quantification of Phenolic Compounds in Olive Oil Based on
31P
NMR Spectroscopy.” 3rd Euro Fed Lipid Congress, September 5-8,
2004, Edinburgh, Scotland.
10.Stella Christophoridou, Photis Dais “A new method for the
determination and identification of phenolic substances in virgin olive
oil by coupling High Performance Liquid Chromatography with Postcolumn Solid Phase Extraction to Nuclear Magnetic Resonance
Spectroscopy (LC-SPE- NMR)” The 1st National conference of Greek
Lipid Forum, Athens Greece, 8-9 June 2005.
11. Stella Christophoridou, Photis Dais, Manfred Spraul
“Identification of phenolic compounds in olive oil by liquid
chromatography with nuclear magnetic resonance (LC NMR).” The 4th
International Conference on Instrumental Methods of Analysis Modern
Trends and Applications, 2 - 6 October, 2005 Heraklion, Crete Greece.
12. P. Dais, S. Christophoridou “Detection and Quantification of
Phenolic Compounds in Olive Oil by Employing 1H High Resolution
Nuclear Magnetic Resonance Spectroscopy. A proposal for on-line
screening” 6th Euro Fed Lipid Congress, "Oils, Fats and Lipids in the
3rd Millennium", 7-10 September 2008, Athens, Greece
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BOOKS AND DISSERTATIONS
•
“Physical Chemistry of Foods”, 2009, Course notes, Technological
Educational Institute of Larissa, Department of Food Technology
•
“Food processing”, 2009, Laboratory notes, Technological
Educational Institute of Larissa, Department of Food Technology
•
“Detection and quantification of polyphenol-containing olive oil model
compounds by employing
31P
NMR spectroscopy.” PhD Thesis
dissertation, 2005.
•
“Development and application of new analytical techniques based on
NMR spectroscopy for the detection and quantification of
polyphenolic compounds in virgin olive oil.” Master Thesis
dissertation, 2001.
TEACHING EXPERIENCE

September 2010 – …
Technological Educational Institute of Larissa, department of
Nutrition and Dietetics, Karditsa, Greece
Course tought: Food Analysis (theory and laboratory)

September 2010 – …
Technological Educational Institute of Larissa, Department of Food
Technology, Karditsa, Greece
Course tought: 1) Physical Chemistry of Foods (theory), 2) Organic
Chemistry Laboratory.

September 2008 – February 2009
Technological Educational Institute of Larissa, Department of Food
Technology. Karditsa, Greece
Course tought: 1) Physical Chemistry of Foods (theory),
2) Laboratory of Food Analysis II, 3) Laboratory of Technology and
Quality control of Oils and Lipids, 4) Food Processing Laboratory II,
5) Laboratory of Food Chemistry
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
September 2008 – February 2009
Department of Biochemistry of Biotechnology, University of
Thessaly, Larissa,Greece.
Course tought: General Chemistry (theory and laboratory)

September 2005 – June 2006
Technological Educational Institute of Crete, Department of natural
resources and environment, Chania, Crete, Greece.
Course tought General Chemistry Laboratory.

September 2000 – January 2001
Chemistry Department, University of Crete, Heraklion Crete,
Greece.
Teaching assistant in the undergraduate Inorganic Chemistry
Laboratory.
DISSERTATION SUPERVISION

Co-supervision: Aswnitou Elsa (2001), Tsirwni Vagia, Kokolaki
Sofia
(2002),
Βίγλη
Γεωργία,
Φιλιπίδης
Άγγελος
(2004),
“Polyphenolic content analysis in virgin olive oils using Ultraviolet–
visible spectroscopy”, Chemistry Department, University of Crete.

Member of the 3 memberd advisory committee of Undergraduate
Dissertation for the Technological Educational Institute of Larissa,
Department of Food Technology:
o Alimpante Aggeliki “Removal of polyphenols from olive mill
wastes using chemically clean methods for the creation of valueadded food products”,
o Koutrou Politimi, “Removal of polyphenols from wine wastes
using chemically clean methods for the creation of value-added
food products.”
o 2010-... Anni Kontou “Impact of genetic characteristics to the
nutritional value of fasoleus vulgaris”.
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o Bosmali Eirini “Impact of genetic characteristics to the
antinutritional factors of fasoleus vulgaris”

Supervising, Panagiotidou Sofia-Maria, tittle “Composition and
oxidative stability of egg yolk originating from organic and non
organic eggs”
INVITED LECTURES

Max-Planck-Institut für chemische Ökologie, Jena, Germany: “NMR
Methodologies for the Analysis of Minor Food Components”:
18/9/2008
•
Institute of Functional Genomics University of Regensburg,
Regensburg, Germany: 28/7/2007
•
1st International Competition of Food Science and Nutrition which
took place on Aristidis Daskalopoulos Federation, “Separation and
Identification of Phenolic Compounds in Olive Oil by Coupling High
Performance Liquid Chromatography with Post-column Solid Phase
Extraction to Nuclear Magnetic Resonance Spectroscopy (LC-SPENMR).” : Athens, 31 October, 2006
GENERAL
Member of the Association of Greek Chemists (E.E.X.).
Member of the Greek Lipid Forum
ECDL
in
Information
Word
and
Processing,
Spreadsheets,
Communication,
Using
the
Presentations,
Computer
and
Managing Files
Languages:
Greek
(mother language)
English
excellent (TOEIC 920)
Italian
fair
German
fair
Dr. Christophoridou Styliani
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References
Professor Photis Dais
Director of the NMR Laboratory
University of Crete
Department of Chemistry
P.O. Box 2208
Voutes, 71003 Heraklion
Crete, Greece
Tel.+30.2810.545037, +30.2810.545047
Fax +30.2810.545001
e-mail dais@chemistry.uoc.gr
http:/www.chemistry.uoc.gr
Dr. Euripides G. Stephanou
Professor
of
Environmental
Chemistry
Environmental Chemical Processes Laboratory
School
of
Sciences
and
Engineering
University of Crete
PO Box 2208
71003 Voutes, Heraklion,
Greece
Tel. +30 2810 545028/545079
Mobile: +30 6936996075
Fax +30 2810 393678
e-mail: stephanou@chemistry.uoc.gr
http://ecpl.chemistry.uoc.gr
Spiros A. Pergantis
Assistant Professor of
Analytical Chemistry
Department of Chemistry
University of Crete
PO Box 2208
71003 Voutes, Heraklion,
Greece
Tel. +30 2810 545084
Fax +30 2810 545001
e-mail:
spergantis@chemistry.uoc.gr
Website: http://www.chemistry.uoc.gr/spergantis/
Dr. Christophoridou Styliani
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15/02/2016
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