DNA Sequencing Methods

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Unit 12 DNA Sequencing Methods

Name ______________________ ( Save file with your name at the end ) Points: ________/50

NOTE: Please use bullets for the list of steps in the procedure. The helpful notes are for your use and are ungraded.

Direct

Sequencing

Chemical

Sequencing

(Maxam-

Gilbert)

Type

Manual method

6.5 points

Dideoxy Chain termination

Sequencing

(Sanger)

Dye Primer – a type of

Dideoxy Chain termination

Sequencing

Manual method

6.5 points

Automated

Fluorescent

Sequencing

Method

6.5 points

Dye terminator type of

Dideoxy Chain termination

Automated

Fluorescent

Sequencing

Method

Sequencing 6.5 points

Pyrosequencing 6.5 points

Bisulfite DNA sequencing or methylationspecific sequencing

Modification of chain termination

6.5 points

Briefly describe and list the steps in the procedure.

Sequencing Briefly describe and list the steps in the procedure.

RNA sequencing 3.5 points

Next Generation Sequencing

3.5 points – Description only

Project

Hierarchal Shotgun sequencing 2 points

Whole-genome Shotgun

Sequencing 2 points

NIH (National Institute of

Health)

Institution

Human Genome Project

BRIEF Description

TIGR (The Institute for

Genomic Research) and

Celear

Helpful Notes

Helpful Notes

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