300417 VO Methoden in der Zellbiologie
Advanced Methods in Cell Biology
SS 2010, 2.0 Std
Roland Foisner, Andreas Brachner, Josef Gotzmann, Gang Dong, Christopher Gerner
1st Meeting (Vorbesprechung): Wednesday March 3, 2010, 2:00 pm, seminar room 3rd
floor, MFPL, Dr. Bohr-Gasse 9, 1030 Wien
The lecture series will take place May 5 to June 7, each Monday and Wednesday 2:00 – 4:00
pm in MFPL seminar room 3rd floor.
VO,
Mo 2.03.09
Wed.
May 5
1.
Topic
Vorbesprechung
Cell clulture and protein expression :
Mon.
May 10
2.
Gene Silencing techniques
 RNA-Interferenz; Antisense, morpholino, epigenetics
Josef Gotzmann
Wed.
May 12
3.
Mon.
May 17
4.
Light Microscopy:
Josef Gotzmann
Wed.
May 19
5.
Wed.
May 26
6.
DNA-Protein interactions:
Mon.
May 31
Tumorbiology techniques
 Cell culture, EMT, telomeres / FISH, DNA
damage, transgenic and knock out tumor
models
Proteomics:
7.
Wed.
June 2
8.
Mon
June 7
9.


Primary cultures, organtypical cultures, differentiation
models, eukaryotic expression systems, vector systems

In vitro transcription und translation, In vitro binding
assays, affinity (Scatchard), In situ analyses (IP), in vivo
analyses, FRET, yeast two hybrid
Bandshift, Footprinting, Chromatin-IP, Yeast one-hybrid
Reporter Gene Assays
Cell Cycle analyses:


Andreas
Brachner
Andreas
Brachner
Roland Foisner
Cell Cycle Stages (FACS, Elutriation, BrdU
incorporation, proliferation marker, synchronization
Protein-identification (MALDI), mass spec, 2D-PAGE
X-crystallography

Roland Foisner
Thomas Dechat
Immunofluorescence, wide field, confocal, live cell
imaging, FRAP, FRET
Protein-Protein interactions:

Lecturer
Andreas
Brachner
Christopher
Gerner
Gang Dong
Protein purification, Crystallisation, X-ray analyses,
structure determination
Exam: Wed. June 30, 2:00 – 3:00 pm, seminar room 3rd floor. MFPL, Dr. Bohr-Gasse 9