BOT 105 Name: ____________ P LANT B IOLOGY Key______________________ Summer Term 2002 Exam 2 1. Use the following diagram to answer the questions. O2 + 2[H] Enzyme H2 O a. Name this type of reaction Redox or Oxidation – reduction Reaction b. Name the substrate that gets reduced O2 c. Name the substrate that gets oxidized H d. Which side of the equation (Left or Right of the arrow) has a higher Potential Energy? Left 2. Enzymes are biologically essential because: a. They defy the Laws of Thermodynamics. b. They decrease the Activation Energy of reactions. c. They create Potential Energy. d. They increase the Activation Energy of a reaction. 3. In Respiration, the molecule NAD+ serves which of the following two roles: a. Cofactor, electron acceptor b. Enzyme, electron acceptor c. Cofactor, electron donor d. Enzyme, electron donor 4. Describe three major differences between Aerobic and Anaerobic Respiration. Aerobic Anaerobic Oxygen No Oxygen More ATP made Less ATP made Glycolysis, Kreb, ETC Glycolysis Part Occurs in Mitocondria Occurs in Cytoplasm Eukaryotes, not Prokaryotes Both 1 BOT 105 Name: ____________ Key______________________ 5. Answer the following questions about the carbon skeleton of glucose. a. What is the ultimate fate of the six carbons that formed the backbone of the original glucose molecule during Glycolysis? 2, 3 carbon Pyruvates b. What has occurred to those carbons by the end of the Krebs cycle? All turn into CO2 6. Name the process(es) which each of the following statements describes (Choices: Glycolysis, Acetyl CoA conversion, Krebs Cycle, Electron Transport Chain (ETC)) a. Generates reducing power in the form of NADH and FADH2 Krebs Cycle b. Produces ATP Glycolysis, Krebs Cycle, ETC c. Begins and ends with a 4-carbon molecule Krebs Cycle d. A catabolic pathway that breaks down glucose to pyruvate in the cytoplasm. Glycolysis e. The means by which the three carbons from pyruvate enter the mitochondria. Acetyl CoA Conversion f. Involves the process of chemiosmosis. ETC g. A series of oxidation/reduction reactions that result in a proton gradient across the inner membrane of the mitochondria. ETC h. The means by which primitive organisms underwent cellular respiration in a pre-oxygen atmosphere Glycolysis 2 BOT 105 Name: ____________ Key______________________ 7. Describe the purpose of the Electron Transport Chain (ETC). In your answer, define the oxidative phosphorylation and explain why oxygen in needed. The purpose of the ETC is to produce an electrochemical potential gradient of H+ across the membrane. Oxygen is the final electron acceptor in the ETC. And oxidative phosphorylation is making ATP with the potential created by the ETC. 8. Name the specific location(s) of each of the following processes: a. Glycolysis Cytosol / Cytoplasm b. Krebs Cycle Matrix of Mitochondria c. Respiration Electron Transport Chain (ETC) Inner Membrane of Mitochondria d. Light Reactions Thylokoid of Chloroplast e. Calvin Cycle in C3 Plants Stroma of Mesophyll Chloroplast f. Calvin Cycle in C4 Plants Stroma of Bundle Sheath Chloroplast g. Calvin Cycle in CAM Plants Stroma of Mesophyll Chloroplast 9. Using the diagram provided, answer each statement with a number corresponding to the site on the figure. a. 4 or 5 Which number corresponds to plastocyanin, which is reduced by the cytochrome complex and shuttles electrons to Photosystem I? b. __1_ Where do electrons first get energized, corresponding to excitement of Photosystem II by light energy? c. 4 or 5 Where is ATP made? d. __6_ Where do electrons get energized by sunlight a second time, corresponding to Photosystem I? e. 8 or 9 Which number represents the stage at which NADPH is made? 3 BOT 105 Name: ____________ Key______________________ 10. Respond to the following statements with one of these answer choices: ATP, NADPH, NADP, NAD, NADH. a. What molecule is a product of the Light reactions and is responsible for phosphorylation of carbon skeletons in the Calvin cycle? ATP b. What molecule provides energy for the regeneration of RuBP from G3P? ATP c. What molecule is a product of the Light reactions and is responsible for reduction or donation of H+ to carbon skeletons in the Calvin cycle? NADPH d. Which product of the Calvin cycle is used to make NADPH in the light reactions? NADP 11. Extreme Thermophiles, extreme Halophiles, and Methanogens belong in the a. Domain Archaea b. Domain Bacteria c. Domain Eukarya d. Domain Fungi e. Domain Plantae f. Domain Protista 12. Purple Sulfur Bacteria a. are Chemosynthetic autotrophs b. use light energy to break H2O c. use light energy to break H2S d. have Photosystem I but not Photosystem II e. have Photosystem II but not Photosystem I f. b and e g. c and d 13. What is the typical Life Style for most Fungus? a. Sporic b. Gametic c. Zygotic 4 BOT 105 Name: ____________ Key______________________ 14. Answer the following questions as True or False in accordance with the Endosymbiotic Theory. a. False Over time, DNA from the host cell gets transferred to the endosymbiont’s nucleus b. False Endosymbiosis is a relationship that can be described in terms of a host and a parasite c. False All organelles of eukaryotes are thought to have evolved from symbiotic relationships d. True Mitochondria originated from bacteria 15. ________________ are thought to be the photosynthetic partners in the Endosymbiotic formation of modern Eukaryotic plant cells because_____________. a. Chloroplasts; they have Thylokoids b. Cyanobacteria; they have the same pigments as modern Eukaryotic plant cells c. Prochlorates; they have the same pigments as modern Eukaryotic plant cells d. Chloroplats; they have the same pigments as modern Eukaryotic plant cells e. Cyanobacteria; they have Thylokoids f. Prochlorates; they have Thylokoids 16. Lichens undergo meiosis in a. Zygosporangia b. Basidium (Basidiocarp) c. Ascocarp d. Soredia e. Lichens don’t sexually reproduced 17. A symbiotic relationship between plant roots and fungal hyphae in which the hyphae enter the cells of the cortex. a. Lichens b. Endomycorrhizae / VAM c. Ectomycorrhizae d. Yeast e. Both b and c 5 BOT 105 Name: ____________ Key______________________ 18. In symbiotic relationships between plants and fungus, the fungus supplies ___________ to the plant. a. Carbon dioxide and water b. Carbohydrates and vitamins c. Water and minerals d. Secretory acids e. Haustoria 19. Which of these abilities allows lichens to live in harsh conditions? a. absorb minerals from the soil b. live heterotrophically c. dry out completely d. parasitism of higher plants e. rapid growth rate 20. Answer the statements about Brown algae as either True or False. a. True Many have a bladder is used to help the alga stay up-right b. False Brown algae are mainly freshwater algae c. False Carageenan is a product of brown algae d. True Brown algae have cells that are specialized for conducting compounds around its body internally e. True Mannitol is an important carbohydrate for transport 21. In Greens, the pyrenoid is the site of: a. Carbohydrate storage b. Flagellum insertion c. Lipid storage d. Mitosis e. Chloroplast endoplasmic reticulum 22. The Chloroplasts of a Cryptomonad are thought to have been acquired by Secondary Endosymbiosis. Name the symbiont and the evidence presented to support that claim. Red algae is thought to be the photosymbiont in Cryptomonads. Evidence found in their similar pigments and the chloroplasts of Cryptomonads have 3 membranes instead of the normal 2. 6 BOT 105 Name: ____________ Key______________________ 23. Fill in the blanks in the chart. Phylum Haptophyta Haptophytes Bacillariophyta Diatoms Chrysophyta Chrysophytes Phaeophyta Brown algae Pigments Ca, Cc, fucoxanthin Ca, Cc, fucoxanthin Ca, Cc, fucoxanthin Ca, Cc, fucoxanthin Food Flag Chryso 0 or 2 lam. Chryso 0 lam. Chryso 2 lam. Laminarin 0 Cell Wall Cellulose calcified Silica Silica or cellulose Cellulose in algin 24. Name 3 ways in which a typical photosynthetic member of the Euglenophyta is different from a typical photosynthetic member of the Dinophyta. Characteristics Euglenophyta Dinophyta Pigments Ca, Cb, Carotinoids Ca, Cc, Peridinin Flagella 1 functional 2 or 0 Food Storage Paramylon Starch Cell Wall No Cell Wall Cellulose Plates Eyespot Yes No 7