BIOL 1406- 1408 Lab Practical I Review
The practical will have 50 questions which will be fill in the blank and short answer. The
practical exam is worth 30% of your lab grade .
Lab Safety
 Lab safety handout
 Lab safety information in lab manual
Measurement
 Metric conversions
 Convert to and from scientific notation
 Prefixes and their multipliers (10, 100, 0.1, 0.01, etc.) to the base unit
 Be able to identify and properly use laboratory equipment and glassware
 Base units
Scientific method
 Know the summary of the scientific process as well how each step pertained to
our experiment.
 Be able to define theory, hypothesis, null hypothesis, independent and
dependent variables, alternative hypothesis, as well as other bolded terms from
this unit.
 Be familiar with these terms in relation to the experiment performed in class
 Be familiar with the significance of the p-value and know how to interpret it.
Spectrophotometry
 Describe the visible portion of the electromagnetic spectrum
 Describe the principles behind Spectrophotometry
 Know the principle components of the spectrophotometer
 Be able to determine the concentration of an unknown with the use of a
standard curve graph
 Understand protein concentration and how it effects absorbance
 Spectrophotometer calibration – can you do it?
 What is a serial dilution? How is one performed?
 Know the importance of and characteristics of a blank
 Dependant variable
 Independent variable
Acids, Bases, pH
 Know the different methods in which to test for pH
 Be able to define pH
 Define what constitutes an acid and a base
 Be able to define and discuss buffering capacity
 Define anthocyanin
 Know the colors associated with pH scale (see end of review for example)
Macromolecules
 Be able to define and give examples of the four general types of macromolecules
 Be able to identify and interpret the results from all colorimetric tests performed
in lab
 Be able to summarize and/or discuss the results from all exercises
Microscopy & Cytology
 Understand the proper use and handling of the compound microscope
 Be able to identify parts of the compound microscope
 Difference between a compound microscope and a dissecting microscope
 Total magnification ( ocular and objective lenses)
 Microscopic field of view
 Identify and state the function of major structures found in plant and animal
cells
 Define Eukaryotic
 Review photos of cheek and plant cells
Osmosis and Diffusion
Understand the importance of osmosis and tonicity in the function of living cells.
What governs the movement of substances?
Be familiar with reagents used in this lab. Which reagents contained copper? Which contained
zinc?
Definitions:
Osmosis
Diffusion
Hypotonic
Isotonic
Hypertonic
Semi-permeable membrane
This is not a comprehensive list of material that could potentially show up on the
practical. Study all old quizzes and lab questions for each unit. Review the procedures
for each exercise/experiment performed in class. If you have questions ~ JUST ASK!!
Have you visited the lab tutorial website? If not, check it out!
http://iws.collin.edu/mgarcia/1406_Lab/Units/Unit_1_Metric/
http://iws.collin.edu/mgarcia/1406_Lab/Units/Unit_2_Spectrophotometry/
http://iws.collin.edu/mgarcia/1406_Lab/Units/Unit_3_Acids_and_Bases/
http://iws.collin.edu/mgarcia/1406_Lab/Units/Unit_4_Macromolecules/
http://iws.collin.edu/mgarcia/1406_Lab/Units/Unit_5_Microscopy/
http://iws.collin.edu/mgarcia/1406_Lab/Units/Unit_6_Diffusion/