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Type of SOP: ☐ Process ☒ Hazardous Chemical ☐ Hazardous Class
Purpose
Doxycycline is harmful if ingested or absorbed through the skin and may be harmful if inhaled. It causes irritation to the gastrointestinal tract, respiratory tract, skin, and eyes. It is a suspected reproductive toxicant.
Doxycycline is a synthetic tetracycline antibiotic derivative that functions by inhibiting bacterial protein synthesis. It is used to treat a variety of bacterial infections.
Physical & Chemical Properties/Definition of Chemical Group
CAS#: 564-25-0
Class: Harmful by ingestion, irritant, reproductive toxin
Molecular Formula: C
22
H
24
N
2
O
8
Form (physical state): Solid
Color: Yellow
Boiling point: Unknown
Potential Hazards/Toxicity
Doxycycline
1
Modified from SOP templates developed by UCLA EH&S ( http://www.sop.ehs.ucla.edu/ )
Date: Click here to enter a date.

Doxycycline is harmful if ingested or absorbed through the skin and may be harmful if inhaled. It causes irritation to the respiratory tract, gastrointestinal tract, skin, and eyes. It may cause birth defects or other reproductive harm. Symptoms of exposure include nausea, vomiting, diarrhea, anorexia, dermatitis, photosensitivity, pericarditis, hypersensitivity, epigastric burning, abdominal pain, esophageal ulceration, headache, edema, hypertension, and hematologic abnormalities.
Engineering Controls
Chemical fume hood. Adequate exhaust and capture filtration.
Personal Protective Equipment (PPE)
Respirator Protection
If lab personnel would like to use respirator on a voluntary basis, they must be trained and fit-tested by
EH&S. This is a regulatory requirement. ( http://or.ucsf.edu/ehs/8193-DSY/version/default/part/4/data/ )
Hand Protection
Handle with gloves. Nitrile gloves are recommended.
NOTE: Consult with your preferred glove manufacturer to ensure that the gloves you plan on using are compatible with doxycycline.
Refer to glove selection chart from the links below: http://www.ansellpro.com/download/Ansell_8thEditionChemicalResistanceGuide.pdf
OR http://www.allsafetyproducts.biz/page/74172
OR http://www.showabestglove.com/site/default.aspx
OR http://www.mapaglove.com/
Eye Protection
ANSI approved, tight-fitting glasses/goggles.
Skin and Body Protection
Appropriately-fitting lab coat, long pants, closed-toe shoes.
Hygiene Measures
Avoid contact with skin, eyes, and clothing. Wash hands before breaks and after handling.
First Aid Procedures
If inhaled
Move person into fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen.
Consult a physician.
In case of skin contact
Flush with plenty of water for at least 15 minutes while removing contaminated clothing. Consult a physician.
In case of eye contact
Flush eyes with plenty of water for at least 15 minutes lifting upper and lower eyelids and removing contact lenses. Consult a physician.
Doxycycline
2
Modified from SOP templates developed by UCLA EH&S ( http://www.sop.ehs.ucla.edu/ )
Date: Click here to enter a date.

If swallowed
Do not induce vomiting. Never give anything by mouth to an unconscious person. Rinse mouth with water.
Consult a physician.
Special Handling and Storage Requirements
Precautions for safe handling: Avoid contact with skin, eyes, and clothing. Avoid inhalation and ingestion.
Avoid dust formation. Provide adequate exhaust ventilation. Ensure normal measures for preventative fire protection.
Conditions for safe storage: Keep container tightly closed in a cool, dry, and well-ventilated area.
Recommended storage temperature is 2-8 °C. Avoid strong oxidizing agents.
Spill and Accident Procedure
Chemical Spill Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-
8522 (SFGH only)
Spill – Assess the extent of danger. Assist contaminated or injured persons. Evacuate the spill area.
Avoid breathing vapors. If possible, confine the spill to a small area using a spill kit or absorbent material. Keep others from entering contaminated area (e.g., use caution tape, barriers, etc.).
Small (<1 L) – If you have training, you may assist in the clean-up effort. Use appropriate personal protective equipment and clean-up material for chemical spilled. Double bag spill waste in clear plastic bags, label and take to the next chemical waste pick-up.
Large (>1 L) – Dial 9-911 from campus phone or 415-476-1414 from cell phone or 415-206-
8522 (SFGH only) for assistance.
Chemical Spill on Body or Clothes – Remove clothing and rinse body thoroughly in emergency shower for at least 15 minutes. If discomfort persists, proceed to the Emergency Department. If no further discomfort is experienced, have the SDS ready and contact Poison Control Hotline at 1-800-
222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-
1300 during work hours, or 9-911 during non-working hours and weekends.
Chemical Splash Into Eyes – Immediately rinse eyeball and inner surface of eyelid with water for
15 minutes by forcibly holding the eye open. If discomfort persists, proceed to the Emergency
Department. If no further discomfort is experienced, have the SDS ready and contact Poison
Control Hotline at 1-800-222-1222 for further exposure information. Notify your direct supervisor and EH&S at 415-476-1300 during work hours, or 9-911 during non-working hours and weekends.
Medical Emergency Dial 9-911 (campus phone) or 476-6911 (cell phone)
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Doxycycline
3
Modified from SOP templates developed by UCLA EH&S ( http://www.sop.ehs.ucla.edu/ )
Date: Click here to enter a date.

Non-Life Threatening Emergency– Go to Occupational Health Programs (OHP) Clinic, 415-885-
7580, 2330 Post Street, Suite 460 Hours of Operation for Appointments: Monday - Friday
7:30 a.m. - 4:00 p.m. (except Holidays).
Note: All serious injuries must be reported to EH&S at 415-476-1300 within 8 hours.
Needle stick/puncture exposure (as applicable to chemical handling procedure) – Wash the affected area with antiseptic soap and warm water for 15 minutes. For mucous membrane exposure, flush the affected area for 15 minutes using an eyewash station. Page the needle stick nurse by dialing 415-353-7842 (STIC).
Decontamination/Waste Disposal Procedure
Clean contaminated surfaces with soap and water and paper towels. Dispose of the paper towels as hazardous waste.
Safety Data Sheet (SDS) Location
Online SDS can be accessed at http://or.ucsf.edu/ehs/7241-DSY/msds.html
Protocol/Procedure
Quantities covered by this SOP:
______ (g , ml) to _______ (g, ml)
Temperature range covered by this SOP:
__ °C – __ °C
General Overview and Purpose:
Enter the experimental purpose
Procedure:
Enter experimental procedure. You can copy procedure from your lab notebook or from literature.
NOTE
Any deviation from this SOP requires approval from the Principal Investigator.
Doxycycline
4
Modified from SOP templates developed by UCLA EH&S ( http://www.sop.ehs.ucla.edu/ )
Date: Click here to enter a date.