Attachment 1 to MPR#4 Project 2010-707 Investigations into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packing cooked, chilled farmed prawns. Prepared by Carl Paulo, Yasmina Sultanbawa & Sue Poole February 2012 CRC 2010-707MPR 4 1. Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns Introduction Expanded polystyrene boxes have been the method of choice for bulk distribution of cooked, chilled farmed prawns due to their insulation properties. Though still acceptable within the catering sector, there is pressure from the retail sector for a better alternative. This push stems from inherent problems arising from the current practice of packaging 5kg prawns topped with 1kg shaved ice. The use of wet ice by industry is two-fold; to limit bacterial growth by maintaining a chilled environment and to retain quality at point of sale, with a focus on the moist glossy outer appearance imparted by melting ice. Unfortunately, this melted ice water is of major concern within retail chains. During handling and distribution polystyrene is prone to crack and break with subsequent leakage of melt water. Another major cause for rejection of polystyrene by retailers is the lack of systems for disposal and adequate recycling of the bulky boxes. At retail level, the preferred material for packaging would be either cardboard or recyclable plastics. Some farms have recently trialled cardboard cartons for commercial product. However, similar issues to polystyrene were encountered. The design of the cartons allowed for capture of about 5cm of melted ice water. Anything more, or any slight angle of placement, would result in leakage of melt water from carton. Tilted boxes invariably occur during handling especially at the wholesale points in the distribution chain where individual cartons are added to a pallet of other mixed chilled goods, in various sizes, to be transported to specific retail stores. Commonly, prawn cartons are added to the top of pallets and may not sit flat depending on the shapes of the other goods loaded on the pallet. Consequently the likelihood of prawn ‘juice’ contaminating other chilled product (for example dairy) placed beneath prawn cartons is ever present. Hence the industry is seeking alternative packaging for the transportation of produce. To date, the most practical alternative is plastic polypropylene containers with a fitted lid. Successful trials have been carried out by the APFA confirming that containers are leak proof and capable of maintaining chilled temperatures during transportation. From these trials a storage life of 12-14 days was achieved. The polypropylene packaging meets the requirements for recyclability however issues remain surrounding correct disposal. A requirement for recycling is that the containers must be thoroughly rinsed for inclusion in the plastics-recycle load. If not adequately cleaned the resulting odour negates the recyclability of the entire plastics load. The APFA requested that we investigate alternative packaging options to replace expanded polystyrene. Here we document trials to determine the viability of design improvements made by manufacturers of cardboard carton boxes. To date, plastic tubs were unavailable to us for trialling. Fortunately, we have now obtained samples and will be incorporating tubs into future trials to establish their effectiveness and the actual storage life of product in combination with synergistic benefits derived from incorporating antimicrobial treatments. 2 CRC 2010-707MPR 4 2. Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns Materials and Methods Processing and shelf life Trial was conducted at Seafarm’s prawn processing facility near Cardwell (QLD, Australia). Banana prawns (Fenneropenaeus merguiensis) were all from the same pond harvest. Prawns used in the various packaging options trialled were from a single cooked batch and modified atmosphere packed (MAP) prawns from the batch previous. Post processing, cooked chilled prawns were packed into 5 kg boxes with a total of six boxes for each of the packaging options trialled. Boxes had hygienic Thermocron TC temperature loggers (OnSolution, NSW, Australia) placed internally at top and bottom. Loggers had a sampling rate every 15 minutes. Six 4 kg bulk MAP trays were also sourced from Seafarm’s stock of retail products. Boxes and MAP trays were transported to Brisbane (QLD, Australia) through Seafarm’s logistics chain. Three days post-harvest, boxes were transported from truck depot to the research facility inside iced one tonne insulated bins. On arrival at research facility boxes were held at 3°C ± 0.5°C until sampling on days 3, 6, 10, 13, 14 and 17 postharvest. On sampling day a box from each of the packaging options and an MAP tray were analysed for microbial and sensorial changes. Packaging options Two different packaging materials were trialled: Expanded polystyrene approved for seafood air transport (internal dimensions 350x250x165 mm (LxWxH)) (Polyfoam, QLD, Australia), and PET lined cardboard carton (internal dimensions 350x250x165 mm (LxWxH)) (AMCOR, QLD, Australia) Three different internal chilling agents were trialled for each packaging material: Plastic bag lining with 1 kg ice placed on top of prawns prior to sealing bag Plastic bag lining with 1.2 kg ice pack (Gel Ice Company, NSW, Australia) placed on top of sealed prawns, and Absorbent pads (Thermasorb, VIC, Australia) and rigid plastic board placed at the bottom of prawns with 1 kg ice on top. Microbial analysis Prawn samples. Two samples of five whole prawns were taken from each box, one sample from top layer and other from bottom. Prawns were aseptically placed in a stomacher bag (Biocorp, Australia) and homogenized at room temperature for 60 s using a Lab Blender 400, Stomacher (Seward Medical, UK). A sub-sample (10 g) of homogenized whole prawns was transferred aseptically into a filter stomacher bag (PX0020S, Elmex, Japan) containing 90 ml of 0.1% peptone water and homogenized for 60 s using a Lab Blender 400. For microbial enumeration a 1.0 ml sample of serial dilutions (1:10, diluent, 0.1% peptone water) were mixed with 10 ml molten agar using the pour plate method [AS 5013.5-2004]. For Total viable counts (TVC) enumeration, Plate Count Agar (PCA, CM0463, Oxoid, Basingstoke, UK) was used and counts were determined after incubation for 72 h at 30°C. For H2S-producing bacteria (including Shewanella putrefaciens) Iron Agar (IA, CM 867, Oxoid, 3 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns Basingstoke, UK) was used. After setting a 10 ml overlay of molten medium was added. Iron agar plates were incubated for 72 h at 30°C and black colonies formed by the production of H2S were enumerated. Two replicates of appropriate dilutions were enumerated. Microbiological data were transformed into logarithms of the number of colony forming units (CFU/g). All plates were examined visually for typical colony types and morphology characteristics associated with each growth medium. Drip loss and melted ice. 25 ml of liquid was drawn from each box using a sterile plastic pipette into a sterile container. A sub-sample (1 ml) was transferred aseptically into 9ml of 0.1% peptone water and mixed. Microbial, TVC and H2S enumeration was performed as per prawn samples. Sensorial analysis Sensorial qualities of banana prawns were assessed using the Quality Index Scheme for black tiger prawns (cooked) (Australian Seafood Quality Index Manual, 2009) with appropriate modification with respect to colour. Through repeat trials, a preliminary quality index scheme can be developed specific for banana prawns although this has not yet been done. A group of 3-5 panellists trained in the Quality Index method assessed prawns individually and held round table discussions until consensus on each assessable parameter. Any notable changes not described on the black tiger prawn QI Scheme were documented and monitored during storage. Thermal properties Preparation of trial was conducted at 3°C ± 0.5°C. Thermocouple wires were attached to the inside top and inside bottom of two polystyrene and two cardboard carton boxes. 5 kg cooked tiger prawns (Penaeus monodon) sourced from various farms (QLD, Australia) were placed in each box and either 1 kg ice or an ice pack as chilling agent placed on top. Boxes were closed and lids securely taped as per commercial practices. Temperature was recorded with a DataTaker Data Logger DT800 (Thermo Fisher Scientific, VIC, Australia) with a sampling rate of 1 minute. A single thermocouple wire was freely recording ambient room temperature. Boxed prawns were relocated to room temperature for 48 hours. Trial was replicated. 4 CRC 2010-707MPR 4 3. Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns Results and Discussion To limit contamination, and to ensure a starting microbial profile inline with product in the retail sector, packaging of prawns was conducted onsite at Seafarm’s processing facility. No changes were made to Seafarm’s processing practices with the exception of substituting their current packaging material with the various options being trialled. To maintain identical bacterial profiles within boxes prawns used were all sourced from the same cooked batch. Unfortunately, due to the processing setup of the farm, prawns for MAP had to be sourced from the batch previous. However, since MAP prawns were from the same pond harvest and processed identically in the same production line, the starting bacterial profile should be near identical. Effects of handling and temperature on flora changes during the distribution chain were kept identical to those experienced within retail products by utilising Seafarm’s logistic network to transport samples to the research facility. The various packaging options presented were tested on their suitability to meet key industry issues, namely recyclability, maintaining low microbial counts and eliminating leakage. The custom designed PET lined cardboard cartons were proposed by packaging company AMCOR, based on claims that they would solve such issues. An improvement to previous designs, carton boxes were made from a single piece of non-perforated cardboard and PET lined on both sides, affording waterproof capacity to the rim. Dimensions were matched to polystyrene boxes trialled to maintain equal headspace and surface area between packaging materials and product. Chilling agents trialled were aimed towards removing either the use of ice or the melted ice water. On arrival at the research facility some polystyrene boxes were leaking because of splitting and breakage sustained during distribution, confirming industry claims that this malfunction is a frequent occurrence. Continued leaking was observed throughout the shelf life storage trial. No such issues were identified from any of the PET lined cardboard boxes. Though cardboard boxes did not leak other issues inherent in their design were encountered. Carton boxes were devised as a two component system; waterproof casement with fitted lid. This meant that a relatively upright position must be maintained to prevent liquid from pouring over the lip of the casement and running down the side of the lid. Though an improvement to the current carton boxes used by industry, boxes of this design need careful handling and stacking in an upright position and further development work is needed to overcome this. During chilled storage no obvious difference could be identified between microbial counts from polystyrene and cardboard carton boxes but, variations were noticed within individual boxes. It should be noted that microbial counts depicted are best observed as a trend over the total storage period rather than absolute for any one sampling time. This is because the count achieved by the plating method may vary 5 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns by ±0.5 log cfu due to metabolic and physiological factors associated with solid agar plating. Total volatile counts from prawns located on the top layer of the majority of boxes were found to be higher than prawns located at the bottom (Figure 1- Figure 4). This pattern was consistently encountered and found to be independent of packaging material or chilling agents used. However, counts were only marginally different and could be representative of the standard error within laboratory analysis or the result of a limited sampling size or localised concentration in flora populations within prawn samples analysed. Further replicates would need to be performed to confirm if this profile is the naturally prevalent pattern. It is also interesting to note that many of the microbial populations showed a ‘drop’ in organisms present between day 14 and day 17 of storage. This currently is unexplained but does not appear to be directly related to chilling agent nor box type. Again replication of trial would add more information. Very similar trials are being designed in which microbial population shifts are to be determined. Figure 1 - Total viable count on prawns in polystyrene and carton boxes with ice as chilling agent. 8 Polystyrene top Polystyrene bottom Carton top 7 Carton bottom Log CFU/g 6 5 4 3 0 2 4 6 8 10 12 14 16 18 Days from harvest If symbolic of flora evolution during cold storage, these results would run contrary to general industry belief that pooled liquid at the bottom of packaging (composed of melted ice water and drip loss) imparts a higher bacterial count in soaked prawns (pers. comms.). Analysis of liquid from all boxes during the storage trial (Figure 5) found this not to be the case. Microbial loads remained relatively constant at around 105 CFU/g until day 13 where a sharp increase was noticed. Towards end of storage micro counts from several prawn samples were found to be well above 106 and some over 107 CFU/g. Contrarily, micro loads of liquid from corresponding boxes were found to be lower with some counts upwards of a log scale different. 6 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns Figure 2 - Total viable count on prawns in polystyrene and carton boxes with ice pack as chilling agent. 8 Polystyrene top Polystyrene bottom Carton top Carton bottom 7 Log CFU/g 6 5 4 3 0 2 4 6 8 10 12 14 16 18 Days from harvest (Fig 2. The Day 17 data point for polystyrene is missing as was incorrectly packaged on-farm) Figure 3 - Total viable count on prawns in polystyrene and carton boxes with ice as chilling agent and absorbent pads. 8 Polystyrene top Polystyrene bottom Carton top 7 Carton bottom Log CFU/g 6 5 4 3 0 2 4 6 8 10 12 14 16 18 Days from harvest 7 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns This documentation of flora evolution supports the idea that there will be potential benefits gained by topical applications of an antimicrobial solution during packaging. As generally expected, microbiological counts for MAP prawns were lower than for prawns from other packaging options. Shelf life extension through MAP was quite obvious with counts < 106 CFU/g throughout storage. Unfortunately, due to the high capital required to install and maintain a MAP system this option would only be viable for a select few growers/processors within the industry. Figure 4 - Total viable count of MAP prawns. 8 MAP top MAP bottom 7 Log CFU/g 6 5 4 3 0 2 4 6 8 10 12 14 16 18 Days from harvest Comparisons between chilling agents identified a distinct suppression of microbial growth during cold storage when gel packs were substituted for ice. Differences in counts of up to one log unit were identified towards end of storage. This result highlights that ice is a secondary source for bacterial contamination and is a less efficient inhibitor of growth, most likely due to a relatively faster melting time. With sensorial analysis, no discernable difference could be found by visual comparison of prawns chilled by ice or with gel packs. Prawns from boxes containing gel packs were described as having superior flavour and texture. This has been attributed to the fact that no melting ice water is present to leach out flavours and salt. Though an extension in shelf life whilst maintaining quality was achieved, when the idea was presented to industry, the cost of implementation was found to be unviable. A cheaper alternative to the polyethylene sealed gel packs trialled are dry sheets requiring hydration prior to freezing. However, previous trials conducted by industry (Lewis, APFA, pers. comm. 2011) resulted in an inferior product when incorporating hydrating ice sheets. Prawns were described as having a ‘dry’ 8 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns appearance, most likely due to the continual absorption of these sheets, which led to the abandonment of further research. Unfortunately this leaves wet ice as still the only feasible option to provide a chilled environment within packaging during cold storage. A suitable replacement for wet ice is an issue being worked on globally with no commercially viable alternatives found to date (Paw Dalgaard, Denmark,pers comm, 2011). Inclusion of pads to absorb melted ice water with a floating semi-rigid plastic sheeting to separate prawns from pads and ice water was also trialled. Though enough pads were used to absorb the melted ice water twice over, liquid was still pooling at bottom of boxes. On further investigation, pads were observed to release captured moisture when excessive force is applied on top, such as 5kg of prawns. To be of functional benefit to industry an absorbent pad robust enough to retain moisture under pressure would need to be developed. Consultation with manufacturers yielded no such product available. Figure 5 - Total viable count of pooled liquid at bottom of boxes. 8 Polystyrene with ice Carton with ice Polystyrene with ice pack Carton with ice pack 7 Polystyrene with absorbent pad Carton with absorbent pad MAP Log CFU/g 6 5 4 3 0 2 4 6 8 10 12 14 16 18 Days from harvest Another major requirement for packaging materials of chilled products is to offer an acceptable level of insulation from temperature abuse. Figure 6 and document the changes in internal temperatures between chilled polystyrene and carton boxes when subjected to thermal abuse. Figure 7 Once exposed to room temperature an immediate rising of internal temperatures in both packaging materials was noticed. Polystyrene quickly rose to an internal temperature of 5-7°C and cardboard cartons rose to 10-13°C. Though the initial rise in temperature experienced inside carton boxes was higher than that of polystyrene the rate at which the internal temperature was warming remained relatively 9 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns constant between packaging materials irrespective of chilling agents used. This constant rate of warming suggests that the carton boxes used offer similar insulation to that of polystyrene under certain environments. However, further design improvements by AMCOR will need to occur to reduce the initial spike in internal temperatures experienced during first exposure to thermal abuse. Comparison of chilling agents identified ice packs as the better performer in maintaining a chilled environment. Figure 6 - Insulation profile of polystyrene and carton boxes with either ice or ice packs as chilling agents (Replicate 1). 30 25 20 Temperature (°C) Ambient 15 Polystyrene ice top Polystyrene ice bottom Carton ice top 10 Carton ice bottom Polystyrene ice pack top Polystyrene ice pack bottom 5 Carton ice pack top Carton ice pack bottom 0 0 4 8 12 16 20 24 28 32 36 40 44 48 -5 -10 Time (hrs) Figure 7 - Insulation profile of polystyrene and carton boxes with either ice or ice packs as chilling agents (Replicate 2). 10 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns 30 25 Temperature (°C) 20 Ambient 15 Polystyrene ice top Polystyrene ice bottom Carton ice top 10 Carton ice bottom Polystyrene ice pack top 5 Polystyrene ice pack bottom Carton ice pack top Carton ice pack bottom 0 0 4 8 12 16 20 24 28 32 36 40 44 48 -5 -10 Time (hrs) An 8 to 10 hour exposure at room temperature (Figures 8 and 9) was sufficient enough to melt ice and induce a dramatic increase in temperature within the headspace of carton boxes in both replicates and in polystyrene during one of the replicates. It took until the fourteenth hour for ice to melt in polystyrene within the other replicate. This created a large differential in temperatures between the top and bottom of boxes utilising ice. This dramatic change in the rate of warming was not observed in boxes containing ice packs. Instead, a consistent increase in temperature was logged and limited temperature differentials between top and bottom was noticed. An unexplainable observation was the similar melting times of ice within both packaging materials despite the difference in temperatures. 11 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns Figure 8 - First 8 hours insulation profile of polystyrene and carton boxes with either ice or ice packs as chilling agents (Replicate 1). 30 25 20 Temperature (°C) Ambient Polystyrene ice top 15 Polystyrene ice bottom Carton ice top 10 Carton ice bottom Polystyrene ice pack top Polystyrene ice pack bottom 5 Carton ice pack top Carton ice pack bottom 0 0 1 2 3 4 5 6 7 8 -5 -10 Time (hrs) Figure 9 - First 8 hours insulation profile of polystyrene and carton boxes with either ice or ice packs as chilling agents (Replicate 2). 30 25 20 Temperature (°C) Ambient Polystyrene ice top 15 Polystyrene ice bottom Carton ice top 10 Carton ice bottom Polystyrene ice pack top Polystyrene ice pack bottom 5 Carton ice pack top Carton ice pack bottom 0 0 1 2 3 4 5 6 7 8 -5 -10 Time (hrs) Drip water loss was not measured in these trials as the prawns were packed out onsite according to farm standard practice. Hence accurate and initial weight of prawns 12 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns or ice added to the boxes could not be obtained without severe interference with the production line operation. 13 CRC 2010-707MPR 4 4. Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns Situation Summary Historically, chilled prawns were packed out and transported in polystyrene boxes for distribution within the supply chain. The advantages of this packaging form are: boxes are inexpensive; light-weight which minimises transport costs; provided excellent temperature insulation during different stages of transport; and are available in different polymer grades with concomitant differential in cost per unit; the heavier grades demonstrated good integrity of pack though the multiple handling steps in the supply chain. However, polystyrene boxes have several disadvantages: when lighter grade and cheaper polymers are chosen package integrity is easily lost through prawn rostrum damage as well as transport handling damage; medium to light grade polymer material also is porous and so allows leakage of meltwater where flake ice is used as coolant. The major problem with polystyrene packaging is that the box is not recyclable and hence use can incur heavy disposal costs at receival end of the chain. Primarily due to this latter issue, the retail sector is demanding alternative packaging formats, with the major retailers not accepting product if received in polystyrene, although polystyrene boxes currently remain acceptable within the food service sector. Therefore, the industry is seeking alternative packaging options to polystyrene. Cardboard cartons were viewed as a likely functional alternative as they are used widely, and highly successfully, within the horticultural product industry. Packaging companies have developed a range of practical carton types, including products with and without wax coatings to prevent moisture leakage. The latter appears to be a feasible option for the prawn industry however, again they are not readily recyclable and therefore not acceptable to the major retailers. Hence the focus turned to plain cardboard boxes which are available commercially as a two-part product – base and lid – and in flat-pack format to minimise cost of transporting and storage while empty. However, several issues are associated with this packaging format. Inherently cardboard cartons exhibit poor temperature insulation capacity and so there is a need for use of a chilling agent within the pack to hold temperature. The Prawn Industry wish to use flake ice as the chilling medium for maintaining chill temperature along the chain as it is readily available on-farm at packout point, relatively cheap and has excellent chilling properties. Flake ice does have the disadvantage of adding to total package weight which can be significant for air transport although a minor issue in road transport. Flake ice has an added benefit of keeping the prawn product moist during distribution. This causes another issue however, as moisture from melting ice reduces cardboard integrity and results in leakage during transport and storage. Within the trials described in this report, use of coolant pads was investigated with high success with respect to chill temperature maintenance. However, these too have inherent issues associated: they require pre-freezing prior to packout and use which for the volumes of product involved during large harvests commandeers a 14 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns great deal of freezer space. Additionally, they are quite a bit more expensive than flake ice hence adding significantly to the cost per kilo of product. Other issues arising from the use of cardboard cartons include the physical structure of the box. When held perfectly level leakage is minimal in the short term. However, cartons maintained in a level position throughout the multiple transport handling steps is not able to be assured and hence the likelihood of leakage onto other palleted products during final stages of distribution is high. To combat this issue, use of a polyethylene liner within the carton was trialled and reduced leakage to some extent although the heavy gauge material required to be impervious to damage by prawn rostrums is an expensive option and did not negate leakage when boxes were tilted beyond a certain point. The use of plastic liners added a new problem – that of prawns “sweating” during transportation. This phenomenon results in increased ‘slime’ and odour volatiles at package opening creating a negative reaction in the marketplace. Despite the associated issues, some industry members are using cardboard cartons for transport of product, but usually only where the distribution chain is short. Because of the inherent issues with the packaging forms listed above, the prawn industry are still seeking a practical and functional alternative. The industry has recently investigated the use of plastic (polypropylene) tubs with a clip on lid. These are similar to the 10kg plastic pails used many years ago to transport prawns but the shape is rectangular rather than the original tall cylindrical pail which resulted in physically crushed prawns in the bottom 5kgs of product. The advantage of this packaging format is the high integrity of the packaging material – prawn rostrums cannot pierce polypropylene and handling damage to the pack is unlikely. Additionally, leakage issues – even where flake ice is included – are much reduced so long as the lid stays firmly in place. There remain some potential issues to address however: the containers are of solid structure and although stackable still require more space when empty than flat-pack cardboard cartons hence transport and storage costs are higher; in themselves they are not cheap and the temperature insulation ability needs to be established. The material used in construction of these tub containers is able to be recycled within the plastics sector but requires a thorough washing-clean step by the retail sector staff before inclusion with other plastic recyclables. This will require training and monitoring by the retailer management. Currently, some industry members have taken up using these plastic tubs and the wider industry is keen to gain feasibility data on their use, especially with respect to temperature holding capacity and overall cost. Consequently, these tubs will be included in the next storage trials currently being arranged for conduction with industry on farm sites. These scale-up trials will also include optimised antimicrobial solutions that are both commercially feasible and all natural (see Attachment 2) to determine the extension to shelf life of cooked chilled prawns in bulk packs. 15 CRC 2010-707MPR 4 Attachment 1 Investigation into use of PET lined cardboard cartons as replacement for expanded polystyrene boxes for packaging cooked, chilled farmed prawns On-going discussions with packaging companies will continue to seek alternatives feasible for the prawn industry. Additionally, continued dialogue with Paw Dalgaard (Seafood expert, Denmark) is occurring prompted by his raising exactly the same issues on a Seafood Discussion list (December, 2011) seeking any information of alternative systems to the use of wet ice. Paw is known to the PI of this project and is very happy to share information with us and the Australian industry. 5. References AS 5013.5-2004. Food microbiology - Microbiology of food and animal feedings stuffs - Horizontal method for the enumeration of microorganisms - Colony count technique at 30°C. AS 5013.5-2004. Sydney, N.S.W.: Standards Australia International BOULTER, M., POOLE, S. & BREMNER, A. (2009) Australian quality index manual - A tool for evaluating changes in chilled seafood. Fisheries Research and Development Corporation and Sydney Fish Market, 2009. 16