Division of Infection & Immunity INFORMATION FOR THE THIRD YEAR COURSE IN VETERINARY MICROBIOLOGY ACADEMIC YEAR 2005/2006 Items contained within this Course Information Document are sometimes unavoidably subject to change. It is the student’s responsibility to ensure they are aware of the correct lecture, practical, tutorial and examination schedules. Lecture venues may be changed during the Course. The Year Notice boards and email should be checked daily. In light of student feedback and External Examiner comments the following changes have been made. Code of Professional Conduct to Fitness to Practice. You are reminded that you will be expected to have read the above code, sign for it and implemented during your time in the course CONTENTS Page 1 Place of the Course in the BVMS Degree Curriculum 2 2 Aims of the Course 2 3 Learning Objectives of the Course 2 Section 1: Bacteriology Section 2: Virology Section 3: Epidemiology and Control of Infectious Diseases Section 4: Veterinary Public Health, Module 1 4 Components of the Course 3 5 Course Content 3 6 Course Texts, Further Reading and Moodle 8 7 Teaching Hours and Teaching Methods 9 8 Methods of Assessment 9 9 Teaching Unit Instructions 15 10 Other Important Information 15 10.1 Protective Clothing, Health and Safety 10.2 Staff and Student Liaison 10.3 Teaching Staff 10.4 Provisional Timetables for Terms I, II and III 10.5 Examples of recent Degree Examination essay questions 10.6 Examples of multiple choice questions Course Information INFORMATION FOR THE THIRD YEAR COURSE IN VETERINARY MICROBIOLOGY ACADEMIC YEAR 2005/2006 1. THE PLACE OF THE COURSE IN THE BVMS DEGREE CURRICULUM Veterinary Microbiology is the study of infectious diseases caused by bacteria, viruses and fungi. Microbiology is an extremely important element of veterinary medicine since infectious diseases are common in domestic animals. In addition, some of these infections may be transmissible to man. Consequently Public Health is a component of the Microbiology course. The Course is given over all three terms of the Third Year and then some elements are continued in the Fourth Year within the Combined Integrated Course when the infectious diseases of each species of domestic animal are considered in detail. During the Third Year, a proper understanding of Microbiology and Public Health will be obtained by combining the knowledge acquired in Veterinary Pathology, Parasitology and Pharmacology with that obtained from the Microbiology Course itself. 2. AIMS OF THE COURSE a) i) To provide basic knowledge of the microorganisms responsible for infectious diseases of domestic animals. To describe the bacteria , fungi and viruses causing these diseases in relation to their structure, modes of replication and disease producing mechanism. Describe the mechanism of action of antimicrobial agents and how bacteria may resist their action To describe the genetic mechanism leading to cancer development, particularly those mechanisms used by oncogenic viruses. Provide details of how the causes of infectious diseases of animals are identified. To explain the principles of vaccination, epidemiology and control of infectious diseases. To provide an introduction to Veterinary Public Health and Food Technology. To apply the knowledge of animal microorganisms and epidemiology to diseases transmissible to man. To provide an overview of the organisation of public health administration. 3. LEARNING OBJECTIVES OF THE COURSE b) c) d) e) f) g) h) On completing the course you should: a) b) c) d) e) f) g) 2 Have a basic knowledge of the microorganisms responsible for infectious diseases of animals derived from this course and earlier courses. Be able to associate the bacteria, fungi and viruses causing animal disease with the diseases caused. Be able to describe the structure, modes of replication and disease producing mechanisms of these agents. Understand the mechanism of action of antimicrobial agents and how bacteria may resist their action Understand the genetic mechanisms leading to cancer developing particularly those used by oncogenic viruses. Be able to handle microorganisms safely in a laboratory and carry out elementary microbiological procedures. Understand the principles of diagnostic methods used in Veterinary Microbiology. Division of Infection & Immunity h) i) j) k) l) m) 4. Understand the nature of vaccines and the principles governing their use. Have knowledge of the fundamentals of epidemiology and be able to supply examples. Understand the basis for the control of microbial disease, including therapy and the mechanisms of antimicrobial resistance, the use of inoculation, disinfection and vaccine programmes, especially with reference to Notifiable Disease. Have a basic knowledge of the importance of food technology. Understand the basic principles and ethics of Veterinary Public Health and the organisation of Public Health in the U.K. Apply the technical knowledge of microorganisms and parasites gained in the course to date to the control of zoonotic diseases. COMPONENTS OF THE COURSE The Third Year Veterinary Microbiology Course extends the information on the basic knowledge of microorganisms and immunology that was provided in the First Year and Second Year course in Biomolecular Sciences by focusing on the organisms that are responsible for infectious diseases of domestic animals and are capable of causing disease in man. The teaching integrates vertically with the Fourth Year Combined Integrated Course as well as the Biomolecular Sciences course. The recurring emphasis of the teaching is the relationship of the basic biology and molecular characteristics of microorganisms with pathogenesis, recovery, diagnosis, epidemiology and control. The course is given in four sections: Bacteriology (including Mycology) Virology (including prion diseases) Epidemiology and Control of Infectious Diseases Veterinary Public Health (Module 1). 5. COURSE CONTENT Section 1: Bacteriology Course Aims 1. To provide a broad view of bacteriology and mycology in general, and veterinary bacteriology and mycology in particular. 2. To describe the structure, ultrastructure and physiology of bacteria and fungi as an aid to the understanding of their biology, replication and culture. Characters that distinguish them from other infective agents are discussed. 3. To discuss the genetics and molecular biology of bacteria, with emphasis on gene regulation, the transfer of genetic information and genetic variation in bacteria. 4. To discuss the basis for classifying bacteria and fungi into groups and the properties of the major groups with reference to bacteria and fungi of veterinary or public health importance. 5. To give specific information about the action of antimicrobials on bacteria and fungi. This is intended to form a basis for the descriptions of antimicrobial therapy that occur in the third year course in Veterinary Pharmacology. The genetic basis of antibiotic resistance and the transfer of resistance are described. 6. To illustrate the broad mechanisms of pathogenesis of bacterial and fungal diseases, and the non-specific and specific immunological response to bacteria and their role in resistance to and recovery from bacterial infections. 3 Course Information 7. To provide an understanding of the principles of vaccination against infectious diseases. The salient features of different types of vaccine are discussed. Both traditional and experimental bacterial vaccines will be described. 8. To consider the theoretical aspects of the detection and identification of bacteria and fungi and their products. Analysis of the specific immune response to bacteria and fungi and their products in the diagnosis of infection is also considered. 9. To describe, in the practical classes, the basic principles of bacterial culture and bacterial identification. To use simple exercises to develop practical skills in the safe handling of bacterial and fungal pathogens. An approach to problem solving in bacterial diagnosis is developed. The practical classes are intended to supplement formal teaching on bacterial groups and to provide a forum for small group informal discussions. Student Objectives By the end of the course, students are expected to be able to undertake or to know the following: 1. Describe the structure and function of bacterial and fungal cells and their components. Understand the differences between bacteria and eukaryotic cells. 2. Understand the basis of bacterial and fungal growth and replication. Know the basic physiology and metabolism of bacteria and fungi. Know the principles of sterilisation and disinfection. 3. Know the characteristic features of the major bacterial and fungal groups and which bacteria and fungi are of veterinary importance, the diseases they cause and their host range. 4. Understand the basics of bacterial genetics including the mechanisms of gene regulation, mutation and gene transfer and understand the importance of plasmids and bacteriophages. 5. Understand the basis of antimicrobial action and know the broad categories of antimicrobials. Know the basis of antimicrobial resistance, its importance, evolution and spread, and how it is measured. 6. Give an outline of the basic processes involved in the pathogenesis of bacterial diseases including the molecular basis of how bacteria colonise their hosts, resist the defence systems of the host, acquire nutrients and damage the host. Appreciate the role of mycotoxins in disease. 7. Know the innate and acquired host defences and the mechanisms involved in resistance to and recovery from bacterial and fungal infections 8. To understand the principles of vaccination, the different types of vaccine and their advantages and disadvantages. Know examples of current and experimental anti-bacterial vaccines and the diseases they prevent 9. Understand and know the principles of how to identify bacteria and fungi or their products using culture, direct visualisation, metabolic tests, serological and nucleic acid based techniques. 10. Carry out the examination of clinical samples for the presence of bacteria and fungi in the laboratory in a safe manner. Presumptively identify cultures of the commonly isolated bacteria and fungi and know how their identity should be confirmed. Section 2: Virology Course Aims 1. To provide an overview of the structure and mode of replication of viruses. 2. To illustrate the basis of the methods used to diagnose viral infections including tests that can be carried out in veterinary practice. 3. To explain the basis of classifying viruses into groups. The properties of the major groups are discussed by reference to viruses of veterinary importance. Emphasis is given to understanding the mechanisms by which viruses cause disease. 4 Division of Infection & Immunity 4. To develop major themes in virology, including strategies for the survival of viruses, persistent viral infections, cancer-causing viruses, variation in viral antigenicity and pathogenicity, arthropod-borne viruses and emerging viral diseases. 5. To explain the mechanisms by which animals recover from viral infections, particularly through immune responses. 6. To explain the effect of genetic change on virus biology and how this can potentially influence the virulence, antigenicity and the host range of viruses. 7. To illustrate the different types of viral vaccine available and the genetic manipulation of viruses in the production of new generation vaccines. Student Objectives By the end of the course students are expected to be able to undertake or know the following: 1. Describe the principles of virus structure and replication. 2. Know the characteristic features of the major viral groups and the important veterinary viruses within those groups, including the agents causing the spongiform encephalopathies. Understand the different types of relationship that viruses can have with their hosts and how certain viral infections become persistent. 3. Appreciate the mechanisms of genetic change in viruses and the selection pressures that lead to changes in virulence, antigenicity or host range. 4. Know the mechanisms involved in recovery from virus infections. 5. Understand the principles involved in the diagnosis viral infections. 6. Describe the types of viral vaccines available and their relative advantages and disadvantages. 7. Know how recombinant DNA technology may be used to directly modify viral genomes and understand its significance, particularly for vaccination. Section 3: Epidemiology and Control of Infectious Diseases Aims of the course 1. To describe the general principles of epidemiology and consider the mechanisms of spread of some of the more important infectious agents of domestic animals. The different types of epidemiological investigation are studied by consideration and discussion of original publications. An introduction to the epidemiological modelling of infectious diseases is given. 2. The aims of the section on control is to bring together all the earlier work on the description of infectious agents and to introduce the concepts of prevention of disease by general methods such as barrier maintenance, isolation and disinfection. The value of vaccination of individuals and populations in disease control in a veterinary context is discussed. The idea of antimicrobial treatment in prophylaxis, disease eradication and in treatment is discussed. Finally, the design of national, farm or individual household disease control programmes is given. Student objectives 1. To be able to define the principle terms used in epidemiology. To understand the several ways in which epidemiological data are collected and appreciate the relevance of each method. 2. To know the principal methods of spread of infectious disease between individuals, between generations of animals and between populations of animals on a local, national and international basis. 3. To know how infectious agents may persist in a population of carrier animals, in the environment and in vectors and to understand the importance of such survival. 5 Course Information 4. To understand the reasons why some diseases are classed as notifiable and to be able to give examples of how such diseases may be controlled. 5. To understand the ways in which the cycle of infection can be broken in order to control or eradicate infectious disease. 6. To understand the ways in which antimicrobials can contribute to the control of infectious disease through treatment, prophylaxis and in eradication programmes. 7. To understand the importance of isolation and barrier maintenance in the control of infectious disease and to be aware of the importance of specific pathogen free status of individual herds and the National Herd. 8. To know the principles behind the control of animal disease at a UK and EU level and to know of examples of the successful control and eradication campaigns carried out in the UK and EU on Notifiable Diseases. Section 4: Veterinary Public Health, Module 1 Outline of the course This module comprises an intensive short course that emphasises the importance of veterinary public health and draws together information presented at other stages of the curriculum, particularly in the earlier parts of the Veterinary Microbiology course and in the Veterinary Pathology and Veterinary Parasitology courses. It is composed of twelve hours of illustrated lectures and a "syndicate" session where individual topics and problems are discussed in small groups and then presented to all participating staff and students for general discussion. Course aims 1. To demonstrate the importance of the role of the veterinarian in safeguarding public health. 2. To consolidate previous elements in the curriculum relating to public health. 3. To promote the consideration of public health aspects of the epidemiology, diagnosis and treatment of disease in animals. Student objectives 1. Understand the role and organisation of medical and environmental health agencies. 2. Appreciate the ethical and legal obligations of the veterinary profession in relation to public health. 3. Have a working knowledge of the control of zoonotic disease. 4. Understand the principles of food and water safety and the conflicts which can arise between maximising production and maintaining safety. 5. Understand the importance of food and water borne disease in man and animals. 6. Know the main stages in food production and the possible sources of contamination. 7. Appreciate the role of all branches of the veterinary profession in the production of foods of animal origin. 8. Be aware of the causes and effects of drug residues and environmental contaminants in relation to public health. 9. Be aware of the complexities of the legislation relevant to public health and know where to get additional information. 10. Give soundly based advice when presented with a problem related to veterinary public health. 6 Division of Infection & Immunity 6. COURSE TEXTS, FURTHER READING AND MOODLE Recommended Textbook The following textbook is recommended: Concise Review Of Veterinary Microbiology. Quinn, P. et al. 2003. Blackwell Scientific ISBN: 1405108983 Details of other useful texts are given on the Microbiology Moodle site (see below) Moodle Moodle is the University's centrally supported Virtual Learning Environment (VLE). The Faculty Moodle site can be accessed from on- or off-campus at: http://moodle.gla.ac.uk/vet/moodle/ Users should log in with their Novell username and password. After a successful login you will be asked to update your profile. Once you have logged on, you will be able to access individual courses within Moodle, as well as general information, and information relating to EMS, summer vacation scholarships, and career opportunities. Many of the courses require a one-time enrolment key. The enrolment key for Microbiology is VETMICRO3.. Within individual courses, you will find links to lecture notes and presentations, past papers, timetables, and links to relevant websites that have been reviewed by your tutors. Throughout the year your tutors may also add additional Moodle ‘activities’ such as self-test material, assignments, surveys and discussion forums. Within ‘General resources and links for students’, you will find links to courses designed to help you start using Moodle. Moodle replaces the Student Web Server, a 2004-5 archive version of which is still available on- or off-campus at: http://www.gla.ac.uk/faculties/vet/teaching/sws/ You will need to username 'vetstudent' and the password 'jamesherriot' to access this. 7 Course Information 7. TEACHING HOURS AND TEACHING METHODS Section Lectures Practicals, tutorials demonstrations, Student hours Staff hours Student hours Staff hours Bacteriology 21 21 14.25 29.5 Virology 21 21 4.5 19.5 Epidemiology and Control 7.5 7.5 5 5 Veterinary Public Health (Module 1) 9 9 3 18 Total 58.5 58.5 26.75 72 A variety of teaching formats is used. Technical aspects of each area are explained in practical classes and demonstrations. During the course, problem-solving exercises are set at the end of some sections for individual home study or may be given as group exercises in class. Some of these exercises may require reference to material in publications and all will require integration of information from different areas of the course or between courses. All staff are not routinely made aware of Students with Disabilities. This information is considered confidential unless such a person agrees to complete disclosure. Consequently, the onus is on you to make individual staff aware of special needs you may have that impact on a particular method of teaching; staff will attempt to make reasonable adjustments. E.g. you may have a degree of redgreen colour blindness and have difficulty distinguishing certain tissues or cells during microscopy. 8. METHODS OF ASSESSMENT. 8.1 Assessment of Student Learning All the examinations in Veterinary Microbiology in 2005/2006 will be considered as summative assessment, for the Degree Examination: Term 1 Term 2 June Examination Examination worth 7.5 Examination worth 7.5 Examination worth 85 All examination papers are submitted in advance to the External Examiner for their approval. The examinations in Term 1 and Term 2 will be marked internally and the results will be available to the students as provisional Grades. The final Grades will be assessed, and if necessary, modified after consultation with the External Examiner, at the time of the June examination. The External will attend in June and September to oversee the examination, the completion of the Grades and to conduct Pass/Fail and Distinction Orals. If a student fails to complete any of the Examinations then the Code of Practice on incomplete assessment and good cause (University of Glasgow Calendar 2003-2004 pp. 44-46 available in the Library) will apply. Under the Data Protection Act students are entitled to see their examination scripts but staff will not discuss individual scripts in detail. 8 Division of Infection & Immunity Code of Practice on complete assessment and good cause (University of Calendar 2005-2006, Sect. Gen. 44-46). This section sets out the rules covering the treatment of students prevented by good cause from completing an assessment or performing adequately therein. (i) (ii) (iii) For the purposes of this section ‘good cause’ shall mean illness or other adverse personal circumstances serious enough to prevent a candidate from completing a summative assessment at or by the due time; ‘evidence’ shall mean a full medical report, not merely a medical certificate, or a written report detailing the adverse personal circumstances including independent written evidence as appropriate. If a candidate believes that good cause has prevented a piece of summative assessment, including an examination script, being completed at or by the due time or has seriously prejudiced his or her performance in a summative assessment, then he or she must promptly notify the Head of Registry and submit appropriate evidence. Notification later than a week after the due date for completion of the assessment will not normally be taken into account except where circumstances prevented the student from notifying the Head of Registry within this time. The Head of Registry will notify the Teaching Unit. Where the claim for good cause is pursued the evidence submitted shall be considered by the Board of Examiners which shall determine. (a) if the evidence is of sufficient weight to constitute evidence of good cause; (b) if sufficient material has been presented in respect of the summative assessment in question for it to be regarded as completed, rather than merely attempted; in the latter case the assessment shall be treated as though it had been prevented by good cause from being completed. If a candidate fails to complete a summative assessment required by a course without adequate evidence of good cause then he or she shall be awarded zero marks or equivalent for that assessment, and his or her mark or grade for the course shall be calculated accordingly. If a candidate is adjudged by the Board of Examiners to have been prevented by good cause from completing the assessment of the course, then the following arrangement shall apply; (i) The extent of the completion of the assessment shall be determined as a percentage of the totality of summatively assessed work, based on assessment weightings published in the course data-base, required by the assessment scheme approved by the Senate. (ii) The Board of Examiners shall make an overall judgement of the standard reached in the completed part of the assessment, using as far as possible the same procedures as apply to candidates who have completed the assessment. (iii) If the candidate has completed 85 per cent or more of the assessment the Board of Examiners shall recommend the award of an appropriate grade (or no credit) on the basis of the completed proportion. (iv) If the candidate has not completed 85 per cent of the assessment then the candidate shall be regarded as not having attempted the assessment. Second Diet of Examination – September The Grades for the second diet will be based on the single examination unless the student has missed the June examination with good cause. In such a case the total assessment will be based on the results for Term 1, 7.5 Term 2, 7.5 September Examination 85. 9 Course Information 8.2 Examinations and Course Work Term I (Martinmas) Exam (Bacteriology) This examination consists of a Practical Examination and a Written Examination held during the last week of the Term. The Practical Examination (1 hour) consists of four questions in which the student comments on aspects of the practical classes in bacteriology and mycology that are presented. The Written Examination (2 hours) consists of two sections: a Multi-Choice Question (MCQ), suggested time 1 hour, and an Essay section of 2 questions (suggested time 1 hour). In the Essay section there are two parts, each containing two questions and one question from each part should be answered. The marks are allocated in the ratio 3:3:1 for the MCQ: Essay Section: Practical Examination. Term II (Candlemas) Exam (Virology) This examination consists of a Practical Examination and a Written Examination held in the last week of the Term. The format of these examinations is as for the Martinmas Term examinations in Bacteriology. 8.3 June Examination This examination is held at the end of Term III (Whitsun) and the dates are given on the current notice "University of Glasgow - Final Dates Degree Examinations" as well as the 3rd year notice board opposite the TU offices. The June examination in Veterinary Microbiology will consist of the following components: (a) A Practical Examination (1 hour) of four questions. All must be answered. (b) A Written Examination composed of a MCQ Section (1.5 hours) and an Essay Section (1.5 hour) of 3 essays. The essay section is in three parts: Bacteriology, Virology and Epidemiology/Public Health. In each part there is a choice of one question from two. (c) The final grade is obtained by summing the marks from all three examinations. Term 1,7.5; Term 2,7.5; June Exam, 85. The result is expressed as a grade according to the system approved by the veterinary faculty. The aggregation score is used only for the calculation of honours and commendation which is arrived at, at the end of the Complete 5 year Course. (d) All papers are made available to the External Examiner. The External Examiner is specifically requested to examine papers of those candidates achieving less than a Grade D and those with marks at or just below Grade A. The External Examiner has the discretion to adjust the marks awarded in the written Examinations. After the External Examiner has confirmed the marks for the written Examinations in consultation with the Internal Examiner: At the discretion of the external examiner a candidate who has achieved a Grade A, (at a first attempt at the examination) maybe invited to attend a Distinction Oral Examination conducted by the External Examiner and at least one of the Internal Examiners. A Pass with Distinction may be awarded at the discretion of the External Examiner. A distinction indicates that the candidate has a thorough, detailed knowledge of everything required and 10 Division of Infection & Immunity that she/he presented the information in a clear, accurate manner and that in addition she/he has a clear understanding of broader concepts of the subject. candidates with a grade E are required to attend a Pass/Fail Oral Examination conducted by the External Examiner and at least one of the Internal Examiners. candidates with a Grade F, G or N may request an Oral Examination conducted by the External Examiner and at least one of the Internal Examiners. The list of students that are invited to attend, required to attend and who may request, an oral examination will be posted as soon as possible after the marks of the written examination are collated. The list will indicate the time and place to which the students should report for their oral exam. On the posted list, students will be identified by matriculation/exam number. It is the individual responsibility of any student who has opted not to have their marks posted on notice boards to determine if they are invited, required or may request an oral examination by contacting the course leader, members of the teaching team or divisional administrative staff. Oral examinations will normally take place within 2 weeks of the written examination. Students not called for an oral may request an oral examination, if so desired. If the candidates fail the 1st Diet of the examination (June) they may take the resit (2nd Diet) examinations (August/September). The format of the 2nd diet of the examination is the same as the June exam: a written Examination composed of a MCQ Section (1.5 hours) and an Essay Section (1.5 hour) of 3 essays. The essay section is in three parts: Bacteriology, Virology and Epidemiology/Public Health. In each part there is a choice of one question from two. A practical exam of 1h. ALL students who achieve less than a grade D will be REQUIRED to have an oral examination conducted by the External Examiner and at least one of the Internal Examiners. The list of students that are required to attend an oral examination will be posted as soon as possible after the marks of the written examination are collated. The list will indicate the time and place to which the students should report for their oral exam. On the posted list, students will be identified by matriculation/exam number. It is the individual responsibility of any student who has opted not to have their marks posted on notice boards to determine if they are required to attend an oral examination, by contacting the course leader, members of the teaching team or divisional administrative staff. Oral examinations will normally take place within 2 weeks of the written examination. Students not called for an oral may request an oral examination, if so desired. (e) All scripts either for Class or Professional Examinations are marked anonymously, using either exam number or matriculation number as directed by University. (f) Passing the Third Professional Examination. A student must pass the Professional Examinations for all four subjects before being allowed to proceed to Fourth Year Classes. If the candidates fail the examination in June they may take the resit (2nd Diet) examinations in September. The format of the September examination will be the same as the June examination, except that ALL students who achieve less than a grade D will be REQUIRED to have an oral examination conducted by the External Examiner and at least one of the Internal Examiners. Grades awarded at the second diet of the professional examination will in accordance with university policy be capped at Grade D. (g) Location of Examination. The Written Class and Degree Examinations will usually be held at Gilmorehill. The Practical and Oral Examinations will normally be held in Garscube. 11 Course Information Grading The University’s policy on grading has changed and is now as follows, with no EP Grade and only Grades A to D representing passes. There shall be three categories of award Honours, Commendation and Pass. A candidate who is not placed in one of three categories shall have failed the programme. A Distinction may be awarded in an individual part of the examination. 8.3 Marks to Grade Correlation for the Faculty of Veterinary Medicine Primary grade Mark Secondary band Mark Aggregation score BVMS A 70-100 65-69 C 55-64 D 50-54 E 45-49 F 40-44 G 30-39 80-100 75-79 70-74 69 67-68 65-66 63-64 60-62 55-59 54 52-53 50-51 49 47-48 45-46 44 42-43 40-41 35-39 30-34 20 19 18 17 16 15 14 13 12 11 10 9 8 7 6 5 4 3 2 1 Honours B 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 3 1 2 H CR 0-30 Commendation Pass Fail The aggregate scores are used in the overall assessment at the end of the 5 year Course. There shall be three categories of award Honours, Commendation and Pass. 8.4 The External Examiner The External Examiner is: Professor W.A. Ellis, Acting Director, Veterinary Sciences Division, Department of Agriculture and Rural Development, Stoney Road Belfast, N. Ireland, BT4 3SD . 8.5 Prizes Which Will Be Given For Work During The Course There are two special Awards in Veterinary Microbiology: (i) a Silver Medal awarded by The Royal Highland & Agricultural Society to student with the highest marks in the Degree Examination and (ii) the George Slavin Prize awarded to the student with the best aggregate marks of the two Class Examinations and the Degree Examination. 9. TEACHING UNIT INSTRUCTIONS Students shall be required to comply with such teaching unit instructions as are prescribed by the Course Leader in charge of the course concerned. Such instructions may require students: to attend 12 Division of Infection & Immunity specified lectures, tutorials, laboratory or practical sessions, examination and other events; to submit items of work, including essays, dissertations and project reports, by such dates as may be instructed. All such instructions shall be given to the students in writing at the beginning of the course concerned. Reasonable notice of any alteration to them will also be given. A student who fails to comply with such instructions may be refused enrolment in and admission to degree examinations in the subject. 10. OTHER IMPORTANT INFORMATION. The following additional information is useful and important. 10.1: Protective Clothing, Health and Safety The study and practice of Microbiology unavoidably involves being exposed to and handling animal tissues and fluids that may contain microbiological agents that are harmful for human beings and other animals. Some of the instruments, equipment and laboratory reagents that are used in this work are also potentially dangerous. Students are expected therefore not only to wear the appropriate protective clothing recommended but also to adjust their own dress suitably and to be responsible in their behaviour when taking part in practical classes held in laboratories. For all practical classes and demonstrations held in the Microbiology Teaching Laboratory, laboratory coats must be worn. Students who consider that handling infectious organisms may damage their health, for example if they are receiving certain medical treatments, should consult the Course Coordinator in confidence. Students will not be allowed to attend practical classes unless the appropriate protective clothing is worn. Drinking or eating (including chewing gum) is not permitted in the laboratory. 10.2: Staff-Student Liaison The staff teaching the Course welcomes the opportunity to discuss problems associated with the course with the students concerned and you are invited to do so. It has been found useful however to have members of staff who pay particular attention to this matter and for the Veterinary Microbiology course these are Professors Mark Roberts and David Taylor and Dr Lesley Nicolson. 13 Course Information 10.3: Teaching Staff The academic staff involved in teaching, examining and administering the Veterinary Microbiology course is: Prof Mark Roberts, Course Leader, Prof David Taylor, Dr Paul Everest, Prof Willie Donachie, Prof David Smith, Dr Lesley Nicolson, Dr Diane Addie, Prof Saveria Campo, Prof James Neil, Dr Brian Willett, Dr Steve Dunham, Dr Adrian Philbey, Dr Linda Scobie, Dr Dom Mellor, Prof Bill Reilly, Mr W Steele. 14 Division of Infection & Immunity 10.4: PROVISIONAL TIMETABLES.. TERM I (MARTINMAS) 2005/2006 BACTERIOLOGY THIS TIMETABLE IS PROVISIONAL – SEE 3RD YEAR NOTICEBOARD/INTRANET FOR CURRENT VERSION Wk 1 Tue Date 27 Sept 2005 Time 0930-1000 1000-1100 1130-1230 Subject Intro to the Microbiology Courses Overview 2nd year work Bacterial identification L L L MR MR DT Venue LAD LAD LAD Mon 03 Oct 2005 0930-1030 1030-1300 Overview of bacterial pathogenesis Practical L P MR MR/DT/PE LAD TL Tue 04 Oct 2005 0930-1030 1030-1300 Antimicrobial action and resistance Practical L P MR MR/DT/PE LAD TL Mon 10 Oct 2005 0930-1030 1030-1300 Staphylococci and Streptococci Practical L P MR MR/DT/PE LAD TL Tue* 11 Oct 2005 0930-1030 1030-1300 Mycobacteria and related bacteria Practical L P PE MR/DT/PE LAD TL Mon* 17 Oct 2005 0930-1030 1030-1300 Clostridia and Bacillus Practical L P PE MR/DT/PE LAD TL Tue 18 Oct 2005 0930-1030 1030-1300 Pasteurellaceae Practical L P MR MR/DT/PE LAD TL Mon 24 Oct 2005 0930-1030 1030-1300 Salmonella and E.coli Practical L P WD MR/DT/PE LAD TL Tues 25 Oct 2005 0930-1030 1100-1200 1200-1300 Gram positive rods Anaerobic Gram negative bacteria Regency meetings L L PE MR LAD LAD Mon 31 Oct 2005 0930-1030 1030-1300 Small G -ve bacteria Practical L P MR MR/DT/PE LAD TL Tue 01 Nov 2005 0930-1030 1030-1300 Enterobacteria + Pseudomonads Practical L P PE MR/DT/PE LAD TL 7 Wed 9 Nov 2005 0930-1030 1030-1300 Fungi Practical L P DT MR/DT/PE LAD TL 8 Wed 16 Nov 2005 0930-1030 1100-1200 Recovery from bacterial infections Bacterial Vaccines L L MR PE AH AH 9 Wed 23 Nov 2005 0930-1030 1100-1200 Obligate intracellular organisms Mycoplasma L L DS DS AH AH 10 Wed 30 Nov 2005 0930-1030 1030-1300 Spirochaetes Practical L P DT MR/DT/PE AH TL 11 Wed 07 Dec 2005 P MR/DT/PE TL 12 Wed Wed 14 Dec 2005 14 Dec 2005 1000-1200 Practical revision class NB exam arrangements are PROVISIONAL 0930-1130 Class Examination (Written) 1430-1630 Class Examination (Practical) 2 3 4 5 6 Main Campus - TBA TL L = Lecture, P = Practical, MR = Prof Mark Roberts, DT = Prof David Taylor, PE = Dr Paul Everest, WD = Prof Willie Donachie, DS= Prof David Smith. Venues: LAD = Lge Animal Dem , AH = Assembly Hall, TL = Preclinical Teaching Laboratory. Course Information TERM II (CANDLEMAS) 2005/2006 VIROLOGY THIS TIMETABLE IS PROVISIONAL – SEE 3RD YEAR NOTICEBOARD/INTRANET FOR CURRENT VERSION Subject Week Date Time Venue 1 Mon Tue 2 Mon Tue 3 Mon Tue 4 Mon Tue 5 9 Jan 10 Jan 16 Jan 17 Jan 23 Jan 24 Jan 30 Jan 31 Jan 0930-1030 Introduction + overview of 2nd year virology L LN AHTC 1100-1230 DNA viruses: Circo-, parvo-, adenoviruses L LN AHTC 0930-1030 How animals recover from viral infections L BW LAD 1100-1230 Diagnosis of viral infections-1 L DA LAD 0930-1030 DNA viruses: Papillomaviruses L SC AHTC 1100-1230 Diagnosis of viral infections-2 L DA AHTC 0930-1030 DNA viruses: Asfar-, poxviruses L SD LAD 1100-1230 Diagnosis of viral infections-3 P DA LAD 0930-1030 DNA viruses: Herpesviruses L LN AHTC 1100-1230 RNA viruses: Picorna-, caliciviruses L LN AHTC 0930-1030 RNA viruses: Flavi, togaviruses L LN LAD 1100-1230 RNA viruses: Nidovirales L DA LAD 0930-1030 RNA viruses: Orthomyxoviruses L LN AHTC 1100-1230 RNA viruses: Paramyxo-, rhabdoviruses L AP AHTC L LN LAD L SD LAD 0930-1030 1100-1230 RNA viruses: Reo,birnaviruses RNA viruses: Retroviruses Mon 6 Feb 0930-1230 Tutorial preparation (self-directed) Tue 7 Feb 0930-1030 Viral Vaccines L SD LAD 1100-1230 The spongiform encephalopathies L AW LAD 6 Wed 15 Feb 1030-1230 7 Wed 22 Feb 1030-1230 8 Wed 1 Mar 1030-1230 Tutorials Tutorials Tutorials T T T NB exam arrangements are PROVISIONAL 9 Wed 8 Mar 1030-123 Class examination (Written Main Campus 9 Wed 8 Mar 1430--1630 Class examination (Practical) TL L = Lecture P = Practical T = Tutorial, DA = Dr Diane Addie SC = Prof Saveria Campo, SD = Dr Steve Dunham, LN = Dr Lesley Nicolson AP = Dr Adrian Philbey, BW = Dr Brian Willett, AW = Dr Alun Williams LS = Linda Scobie. Venues LAD = Large Animal Demonstration Lab; TL = Preclinical Teaching Laboratory, AHTC = Animal Health Technology Centre. 2 Division of Infection & Immunity TERM III (WHITSUN) 2005/2006 EPIDEMIOLOGY AND CONTROL OF INFECTIOUS DISEASES THIS TIMETABLE IS PROVISIONAL – SEE 3RD YEAR NOTICEBOARD/INTRANET FOR CURRENT VERSION Wk 1 Date Mon Tue 2 Mon Tues 10 Apr 2006 11 Apr 2006 17 Apr 2006 18 Apr 2006 Time Subject Venue 0930-1030 2: Epidemiology of bacterial diseases L AHTC DT 1100 -1200 3: Epidemiology of viral diseases L AHTC SD 0930-1030 Control of infectious diseases, 1: Overview L AHTC DT 1045-1245 2: Maintenance of disease-free stock. Antimicrobials in disease control. L AHTC DT 0930-1030 Introduction to Food Technology L AHTC DT 1100-1230 Practical Food Technology P TBA DT, MR, PE 0930-1000 Epidemiology of infectious diseases: L AHTC DM D AHTC DM Epidemiological methods 1030-1130 Epidemiological modelling for disease control L = Lecture, P = Practical, D = Demonstration, Dr Dominic Mellor, SD =. Dr Steve Dunham , MR = Prof Mark Roberts, DT = Prof David Taylor, PE = Dr Paul Everest,. Venues: LAD = AHTC = Animal Health Technology Centre VETERINARY PUBLIC HEALTH (MODULE 1) Venue 3 4 5 Mon 24 Apr 2006 Regency day Tue 25 Apr 2006 Mon 1 May 2006 Tue 2 May 2006 0930-1230 Mon 8 May 2006 Tue 9 May 2006 0930-1230 Veterinary Public Health-1 L AHTC WJR Veterinary Public Health-2 L AHTC WJR 0930-1230 Veterinary Public Health-3 L AHTC WJR 0930-1230 Veterinary Public Health-2 L AHTC WJR University Holiday A separate timetable with details of the course will be posted. WJR = Prof WJ Reilly REVISION Venue 6 Wed 17 May 2006 1030-1230 Microbiology practical revision P TL DT, MR, PE DEGREE EXAMINATION 7 Wed 24 May 2006 0930-1230 Microbiology Written Exam Wed 24 May 2006 1430-1630 Microbiology Practical Exam 3 Division of Infection & Immunity 10.5: EXAMPLES OF CLASS EXAMINATION PAPERS Wednesday 4 September 2002 0930-1230 THIRD PROFESSIONAL EXAMINATION FOR THE DEGREE OF B.V.M.S. VETERINARY MICROBIOLOGY SECTION 1 ESSAYS Answer THREE questions: ONE from Part A, ONE from Part B and ONE from Part C. Write your answer to each question in a separate book. Note that the allocation of marks to parts of questions is given as a guideline only and may not be strictly adhered to in marking. PART A. BACTERIOLOGY 1 Describe the morphological and cultural characters of Clostridium tetani. Describe how Clostridium tetani can cause fatal disease in the horse. Outline methods by which the disease caused could be treated and prevented. 2 Describe the action of the major groups of antibiotics and how resistance genes in bacteria can counteract these actions. 3 Discuss the importance of fungi in animal disease. PART B. VIROLOGY 4 Variant viruses that arise in animals infected with bovine virus diarrhoea virus, feline leukaemia virus or feline coronavirus may cause disease in that infected individual. Indicate the novel features of these variant viruses and outline the pathogenesis of the diseases that they induce. 5 What factors may influence the ability of viruses to grow in particular cells, tissues or whole animals? Use specific viruses as examples. How can viruses acquire the ability to grow in different cells or hosts? Give examples of the consequences for animal populations that have followed such changes in viruses. 6 Give examples of viruses of domestic or wild animals that cause disease in man, describing how these viruses are maintained in their natural populations and how they may be transmitted to people. PART C. EPIDEMIOLOGY AND PUBLIC HEALTH 7 Give an account of the ways in which Cryptosporidium in water can infect man. Suggest ways in which water contamination may be reduced or prevented. 8 Describe the types of vaccines that are available to prevent bacterial and viral infections of domestic animals. Explain the advantages and potential drawbacks of each type. In future, new vaccines will be required to prevent infectious diseases. Discuss further challenges that we might have to face and explain how vaccines might be developed to overcome these problems. 9 What are the requirements of an employer under the Control of Substances Hazardous to Health (COSHH) Regulations 1999? Describe how this should be implemented for veterinary students undertaking lambing experience. 30 40 30 100 100 100 50 50 100 70 30 60 40 100 Course Information Wednesday 18 June 2003 0930-1230 THIRD PROFESSIONAL EXAMINATION FOR THE DEGREE OF B.V.M.S. VETERINARY MICROBIOLOGY SECTION 1 ESSAYS Answer THREE questions: ONE from Part A, ONE from Part B and ONE from Part C. Write your answer to each question in a separate book. Note that the allocation of marks to parts of questions is given as a guideline only and may not be strictly adhered to in marking. PART A. BACTERIOLOGY 1 A previously unknown Gram-positive bacterium was isolated from the blood of several cattle in the same herd that died from septicaemia. How might it be established that the isolated organism was indeed the cause of the disease in these animals? If the organism was found to be the cause of the disease, suggest ways in which the molecular basis of its pathogenicity could be examined. 2 a) List the characteristics of Escherichia coli , the species it infects and the diseases it cause. b) Describe the pathogenesis of gastrointestinal disease caused by E.coli . 3 Describe, with examples, the different types of vaccines currently used to prevent bacterial diseases of animals. How are advances in biotechnology influencing vaccine development? PART B. VIROLOGY 4 a) Discuss the mechanisms by which antigenic change occurs in influenza viruses and detail the selection pressures that may facilitate emergence of new viral strains b) Describe the role played by avian and mammalian species in the emergence of new strains of influenza virus. 5 What features of the interaction of viruses with their hosts (at the molecular, cellular or whole animal level) might be responsible for the differences in the susceptibility of different species of animals to a virus? Illustrate your answer with examples of veterinary viruses which have distinct clinical consequences in different species. 6 a) Coronaviruses are important pathogens of domestic animals. Give a brief description of coronaviruses, in particular those of pigs, cats and chickens, including the diseases that they cause and the way in which they are transmitted. b) Discuss the possible consequences of genetic change of coronaviruses for humans and animals. PART C. EPIDEMIOLOGY AND PUBLIC HEALTH 7 Discuss the differences in the life cycles of Toxocara canis and Toxoplasma gondii in terms of their public health importance. 8 Describe the procedure adopted when a food poisoning outbreak is suspected. Include in your answer the time required for each stage of the response and the personnel involved. Use E. coli O157 as a prime example, but draw on other information at your disposal. 9 Carcase disposal by burial has been made illegal from the beginning of May, and a collection system will be introduced with final disposal by landfill, rendering or incineration. On farm incineration will still be permitted. Outline the benefits and disadvantages of these measures: a) for animal health b) for Public Health Illustrate your answer with examples 2 100 50 50 100 50 50 100 50 50 100 100 50 50 Division of Infection & Immunity Wednesday 3rd September 2003 0930-1230 THIRD PROFESSIONAL EXAMINATION FOR THE DEGREE OF B.V.M.S. VETERINARY MICROBIOLOGY SECTION 1 ESSAYS Answer THREE questions: ONE from Part A, ONE from Part B and ONE from Part C. Write your answer to each question in a separate book. Note that the allocation of marks to parts of questions is given as a guideline only and may not be strictly adhered to in marking. PART A. BACTERIOLOGY 1 a) Describe the characteristics of the genus Clostridium and list the species of veterinary importance, the host species they affect and the diseases they cause. b) Describe how Clostridium tetani and Clostridium botulinum cause disease. 2 Explain, with examples, how antibiotics are able to exhibit selective toxicity against bacteria. 3 Discuss the role of the innate and acquired immune systems in the resistance to and recovery from bacterial infections. PART B. VIROLOGY 4 a) Describe the general characteristics of herpesvirus virions, genomes and the diseases caused by this family of viruses b) Herpesviruses are subclassified into 3 subfamilies. Describe characteristics of members of each subfamily. c) What strategies do herpesviruses use for persisting in infected hosts? 5 a) Describe viral diseases in the UK and worldwide where transmission by arthropod borne vectors is important. b) Illustrating your answer with examples, describe how arthropod borne diseases are controlled. 6 Severe acute respiratory syndrome (SARS) virus is a coronavirus which emerged suddenly in the human population, the natural host is said to be the civet cat. a) Using your knowledge of veterinary coronaviruses, what would you surmise that the basic structure of the SARS virus would be? b) Which two body excretion samples would you take from a living patient to try to establish where the virus is being shed? Illustrate your answer with examples of coronavirus shedding in other species. What kind of tests would be used to detect the virus? c) Journalists speculate about SARS not being gone for good and that it might re-emerge in the future. State why, in your opinion, healthy human carriers might, or might not, be found. Again, give animal coronavirus examples. PART C. EPIDEMIOLOGY AND PUBLIC HEALTH 7 (a) Discuss the role of wildlife reservoirs in the epidemiology of rabies (b) Outline the implications of these reservoirs for the control of rabies virus in domestic animals and humans 8 Give an account of the way in which bovine tuberculosis spreads within the UK and summarise the methods used in its control. 9 Give an account of the Public Health importance of E.coli 0157 infection in the UK. Include in your answer the sources of the organism for man and possible methods of control. 50 50 100 100 50 20 30 50 50 20 50 30 60 40 100 100 3 Course Information Wednesday 9 June 2004 9.30 – 12.30 THIRD PROFESSIONAL EXAMINATION FOR THE DEGREE OF B.V.M.S. VETERINARY MICROBIOLOGY SECTION 1: ESSAYS Answer THREE questions: ONE from Part A, ONE from Part B and ONE from Part C. Write your answer to each question in a separate book. Note that the allocation of marks to parts of questions is given as a guideline only and may not be strictly adhered to in marking. PART A. BACTERIOLOGY 1. Give an account of the pathogenesis and epidemiology of anthrax. Why is Bacillus anthracis considered to be a potent biological warfare agent? 100 2. A. Describe the genus salmonella in terms of taxonomy, identification, diseases caused, host range and which animals are affected. 70 B. Describe how salmonella cause diarrhoea. 30 PART B. VIROLOGY 3. How do viruses transmit from one animal to another? Give examples of veterinary viruses for each mode of transmission. 100 4. A. Detail the mechanisms by which genetic variation can occur in viruses and discuss the potential effects of virus mutants on animal populations. 60 B. How might emerging viral diseases be controlled? 40 PART C. EPIDEMIOLOGY AND PUBLIC HEALTH 5. Describe the methods used throughout the world to control foot and mouth disease. How and why do control methods differ for regions where the disease is endemic and epidemic? What difficulties are associated with control of an epidemic (highlighted by the recent outbreak in the UK)? 100 6. Compare the foodborne infections caused by Mycobacterium bovis with that caused by Escherichia coli 0157 and outline methods for their prevention. 100 4 Division of Infection & Immunity Wednesday 1st September 2004 THIRD PROFESSIONAL EXAMINATION FOR THE DEGREE OF B.V.M.S. VETERINARY MICROBIOLOGY 0930-1230 Answer THREE questions: ONE from Part A, ONE from Part B and ONE from Part C. Write your answer to each question in a separate book. Note that the allocation of marks to parts of questions is given as a guideline only and may not be strictly adhered to in marking. PART A. BACTERIOLOGY 1 Describe how bacteria can become resistant to the following types of antibiotics: betalactams, tetracyclines and aminoglycosides. How can antibiotic resistance be passed from one bacteria to another? 2 Using examples, outline the mechanisms Clostridium species use to cause disease in animals. 100 100 PART B. VIROLOGY 3 Discuss, with specific examples, mechanisms employed by viruses to evade host immune responses. 100 4 100 In the late 1970s, canine parvovirus emerged as a new infection of dogs. The mortality rate was extremely high, with many dogs dying within hours of first manifesting clinical disease. Nowadays, most cases survive – why do you suppose that is? PART C. EPIDEMIOLOGY AND PUBLIC HEALTH 7 What methods are available to treat and prevent viral diseases in domestic animals? Describe, using examples, how vaccines may be used to control viral diseases in animal populations. 8 Discuss the importance of heating and refrigeration for the prevention of foodborne disease. 100 100 5 Course Information Wednesday 25 May 2005 0930-1230 THIRD PROFESSIONAL EXAMINATION FOR THE DEGREE OF B.V.M.S. VETERINARY MICROBIOLOGY PART A. BACTERIOLOGY 1. Write an essay on Staphylococci. Include in your answer: the basic microbiology of the group; diseases caused and pathogenesis of infection. Give a general account of Yersinia species and their importance in veterinary medicine. 2. PART B. VIROLOGY Pestiviruses and herpesviruses are both associated with important diseases of production 3. animals in the UK. A. Briefly describe the major diseases of production animals that are associated with these viruses B. Contrast the mechanisms by which these viruses persist in a population of animals C. Using one example from each, briefly outline the methods for disease control 4. 40 40 20 20 B. Name three coronaviruses of animals and indicate the diseases that each causes. C. What features of coronaviruses enable them to persist in animal populations? 30 50 A. List the agents present in sheep (or lamb) faeces which could cause zoonotic disease. B. Briefly outline the circumstances in which zoonotic disease could result from exposure to these agents. C. Describe the principles used to prevent human infection from sheep faeces i) in the abattoir and ii) in an open farm 6 100 Coronaviruses. A. Describe the structure of a coronavirus. PART C. EPIDEMIOLOGY AND PUBLIC HEALTH A. Describe the ways in which bacterial respiratory diseases spread. Illustrate your answer 5. with examples B. Suggest methods for the control of bacterial respiratory diseases in animals 6. 100 50 50 20 30 50 Division of Infection & Immunity 10.6 EXAMPLES OF MULTIPLE CHOICE QUESTIONS THIRD PROFESSIONAL EXAMINATION FOR THE DEGREE OF B.V.M.S. VETERINARY MICROBIOLOGY MULTIPLE CHOICE - PART A EDPAC QUESTIONS - Instructions for completion of the PINK answer sheet 1. Use a pencil (HB preferably) 2. Complete the identifying sections at the top of the pink answer sheet. a) Write your name in the top line of boxes as follows: JONES IL b) Pencil in the letters of your name as above with a horizontal line through the small rectangle e.g. [B]. c) Mark your matriculation number in 6 consecutive boxes in the area marked 'CANDIDATE NUMBER' 3. Do NOT write on the form other than in the boxes indicated. 4. You do not have to complete the boxes marked 'SUBJECT' or 'SUBJECT CODE'. Marking Correct answer Wrong answer No answer 1 -1/3 0 1-3 Which of the viruses on the left has the particle structure indicated on the right? (A) Naked, icosahedral capsid (1) Adenovirus (B) Enveloped, icosahedral capsid (2) Flavivirus (C) Naked, helical nucleocapsid (3) Herpesvirus (D) Enveloped, helical nucleocapsid (E) Complex 4-6 Indicate which of the techniques on the right are most relevant to the aims on the left. (A) Capture of viral antigen by a monoclonal antibody (4) Haemagglutination. (B) Growth of virus in cell culture. (5) Immunofluorescence. (C) Visualisation of lesion fluids. (6) Virus isolation. (D) Incubation of fixed, virus-infected cells with antibody. (E) Incubation of sample with a suspension of red blood cells 7 Course Information 7-9 Which of the canine viruses on the left has the properties on the right. (7) (8) (9) Canine adenovirus Canine parvovirus Canine herpesvirus (A) (B) (C) (D) Genome is single stranded DNA Genome is in several fragments Replicates in cytoplasm Envelope is initially acquired from nuclear membrane (E) Fibers project from the capsid 10-12 Indicate which mechanism on the right is associated with the survival of the virus on the left. (A) Persistence in an arthropod vector (10) Feline calicivirus. (B) Continuous excretion from the tonsil (11) Feline herpesvirus. (C) Latent infection in nerve cells (12) Feline leukaemia virus. (D) Very resistant to the environment (E) Integration of proviral DNA into cell DNA 13-15 Which of the attributes on the right is most closely associated with the viruses on the left? (A) Cause lytic infections (13) Alphaherpesviruses. (B) All cause respiratory disease (14) Betaherpesviruses. (C) Grow slowly (15) Gammaherpesviruses. (D) Infect lymphocytes (E) Cause giant cell formation 16-18 Which disease on the left is transmitted by the arthropod vector on the right? (16) (17) (18) Tick. Mosquito. Flea. (A) (B) (C) (D) (E) Myxomatosis Western equine encephalomyelitis Equine infectious anaemia African swine fever Classical swine fever 19-21 Which virus on the left is responsible for the condition in chickens on the right? (A) Marek's disease (19) Circovirus. (B) Infectious bursal disease (20) Herpesvirus. (C) Infectious bronchitis (21) Birnavirus. (D) Chicken anaemia (E) Fowl pest 22-24 Match the specific diagnostic procedure on the left with the most appropriate virus on the right. (A) Feline leukaemia virus (22) Detection of plasma antigen (B) Feline immunodeficiency virus (23) Detection of serum antibody (C) Feline calicivirus (24) Detection of antigen in faeces (D) Feline orthopoxvirus (E) Feline parvovirus 8 Division of Infection & Immunity 25-27 Match the disease on the left with the control measure used in the UK on the right. (25) (26) (27) Rabies Foot-and-mouth disease Marek's disease (A) Vaccination of all susceptible animals (B) Slaughter of all susceptible animals in contact with cases of disease (C) Slaughter of all affected animals (D) Quarantine of affected animals for 6 months (E) Quarantine and vaccination of potentially affected animals 28-30 Match the function on the left with the bacterial structure on the right. (28) (29) (30) Motility Adhesion to mucosal surfaces O-antigenicity (A) (B) (C) (D) (E) Lipopolysaccharide Fimbriae (pili) Peptidoglycan Flagella Lipoprotein 31-33 Match the cultural character on the left with the organism on the right. (31) (32) (33) Swarming on blood agar. No growth on artificial media No growth aerobically (A) (B) (C) (D) (E) Proteus Fusobacteria Salmonella Rickettsia Mycobacterium 34-36 Which antimicrobial on the left has the mode of action on the right (34) (35) (36) Bacitracin Trimethoprim Rifampicin (A) (B) (C) (D) (E) Inhibits cell wall synthesis Inhibits transcription Inhibits translation Inhibits folic acid synthesis Inhibits DNA supercoiling 37-39 Match the organisms on the left with the vaccines on the right. (37) (38) (39) Leptospira canicola Bordetella bronchiseptica Clostridium tetani (A) (B) (C) (D) (E) Live attenuated vaccine Killed alum adjuvanted vaccine In-feed killed vaccine Toxoid vaccine Ribosome vaccine 40-42 Which practice on the right is the most efficient method of carrying out the objective on the left? (40) (41) (42) Stimulation of colostral immunity Stimulation of local gut immunity Producing antitoxic immunity (A) (B) (C) (D) (E) Intramammary inoculation in lactation In-feed administration Using a washed cell vaccine Vaccinating 6 and 2 weeks before parturition Using an adjuvanted toxoid 9 Course Information 43-45 Choose the feature on the right which is most appropriate to the organism on the left. (43) (44) (45) Listeria monocytogenes Clostridium tetani Campylobacter jejuni (A) (B) (C) (D) (E) Strictly anaerobic Strictly microaerophilic Strictly aerobic Grows at 4°C Saccharolytic in cooked meat medium 46-48 Which organism on the left is responsible for the disease on the right? (46) (47) (48) Serpulina hyodysenteriae Clostridium tetani Clostridium perfringens (A) (B) (C) (D) (E) Wooden tongue in cattle Necrotic enteritis of fowls Swine dysentery Tetanus Calf diphtheria 49-51 Match the organisms on the left with the disease on the right. (49) (50) (51) Malassezia pachydermatis Aspergillus flavus Cryptococcus neoformans (A) (B) (C) (D) (E) Favus in the fowl Feline rhinitis Thrush Otitis externa Aflatoxicosis 52-54 Match the bacterial toxin on the left with its action on the right. (52) (53) (54) Tetanus toxin E. coli heat stable toxin E. coli heat labile toxin (A) (B) (C) (D) (E) Increases intracellular cyclic AMP Increases intracellular cyclic GMP Introduces pores into the cell membrane Inhibits neurotransmitter release Inhibits protein synthesis 55-57 Match the organism on the left with a statement on the right. (55) (56) (57) 58. Cryptosporidium Campylobacter Verotoxigenic E. coli Associated with eggs Is not killed by chlorination Associated with soft cheese Can cause kidney failure in humans Most common cause of gastroenteritis in humans Which ONE of the following is an example of experimental epidemiology in veterinary medicine? (A) (B) (C) (D) (E) 10 (A) (B) (C) (D) (E) A randomised clinical trial A cohort study A case control study A survey A cross-sectional study Division of Infection & Immunity 59. Which one of these diseases of sheep is caused by a virus that may produce disease in cattle? (A) (B) (C) (D) (E) 60. Which ONE of the viruses that cause the following diseases of poultry has a segmented double-stranded RNA genome? (A) (B) (C) (D) (E) 61. Maedi-visna Border disease Orf Sheep pulmonary adenomatosis Sheep pox Infectious bursal disease Fowl plague Newcastle disease Infectious bronchitis Marek’s disease Which ONE of these statements most appropriately explains the mechanism of attenuation of viruses by prolonged passage in cell cultures? (A) (B) (C) (D) (E) Selection of mutant viruses. Induction of mutation in viruses Selection of resistant cells. Selection of susceptible cells. Loss of infectivity of virus. 62. Which ONE of these statements applies to rotavirus infections: (A) Actively dividing cells in the crypts of the intestinal villi are destroyed. (B) Differentiated cells at the tips of the intestinal villi are destroyed. (C) Commonly causes disease in geriatric animals. (D) Cannot be controlled within a population by vaccination. (E) Virus is transmitted by arthropod vectors. 63. Which ONE of these agents is directly transmissible to man from sheep? (A) (B) (C) (D) (E) 64. Flavivirus Scrapie Parapoxvirus Lentivirus Paramyxovirus Which of the following viruses is commonly associated with reproductive failure ? (A) (B) (C) (D) (E) Feline immunodeficiency virus Feline coronavirus Porcine parvovirus Equine influenza virus Fowlpox virus 11 Course Information .65. Which ONE of the following statements is most appropriate to Clostridium perfringens? (A) (B) (C) (D) (E) 66. By which ONE of the following methods does Bacillus anthracis survive between hosts? (A) (B) (C) (D) (E) 67. Guttural pouch mycosis in the horse Avian respiratory mycosis Subcutaneous dermatomycosis in the dog Canine nasal mycosis Abortion in cattle. Which ONE of the following organisms would you expect to be sensitive to penicillin? (A) (B) (C) (D) (E) 12 Produces spiral hyphae Produces pink colonies with a powdery border Produces spindle-shaped macroconidia in vitro Is a possible contributor to otitis externa in the dog Is susceptible to oxytetracycline spray Which of these diseases is NOT associated with Aspergillus spp.? (A) (B) (C) (D) (E) 70. For the differentiation of mastitis-associated streptococci For the differentiation of staphylococci For the differentiation of Actinomyces pyogenes For the selection of Salmonella For the differentiation of Salmonella from E. coli Which ONE of the following responses is correct? Microsporum canis: (A) (B) (C) (D) (E) 69. As a dried vegetative cell In soil as a spore In water In semen In milk Which ONE of the following statements is correct? Edward's medium is used: (A) (B) (C) (D) (E) 68. Gram negative rod Grows aerobically on chocolate agar Produces a variety of exotoxins Is proteolytic in cooked meat medium Causes braxy E. coli S. typhimurium Chlamydia psittaci E. canis S. equi subsp. equi Division of Infection & Immunity 71. Which ONE of the following syndromes is associated with Haemophilus parasuis? (A) (B) (C) (D) (E) 72. Which ONE of the following bacteria produces an enterotoxin? (A) (B) (C) (D) (E) 73. Salmonella pullorum Enterococcus faecium Staphylococcus aureus Streptococcus equi Lactobacillus acidophilus To which ONE of the following does "Duty of Care" for the disposal of clinical waste NOT apply? (A) (B) (C) (D) (E) 74. Pleuropneumonia Erysipelas Atrophic rhinitis Glasser's disease Greasy Pig disease Pet animal carcases Pet animal faeces Farm animal carcases Soiled swabs and dressings Used syringes and needles Which ONE of the following is the most frequently isolated Salmonella from people in the UK? (A) (B) (C) (D) (E) Salmonella agona Salmonella hadar Salmonella enteritidis Salmonella bongori Salmonella virchow 13 Course Information MULTIPLE CHOICE - Part B TRUE/FALSE QUESTIONS Instructions for completion of the WHITE answer sheet 1. Use a pencil (HB preferably) 2. Complete the identifying sections at the top of the green answer sheet. a) Write your name in the top line of boxes as follows: JONES IL b) Pencil in the letters of your name as above with a horizontal line through the small rectangle e.g. [T]. c) Mark your Matriculation Number in 6 consecutive boxes in the area marked 'CANDIDATE NUMBER' 3. Do NOT write on the form other than in the boxes indicated. 4. You do not have to complete the boxes marked 'SUBJECT' or 'SUBJECT CODE'. 5. Answer (T), (F) or (?). Marking Correct answer Wrong answer No answer (?) 1 -1 0 1. Indicate whether TRUE or FALSE. Viral envelopes have the following characteristics. (1) Never formed at the nuclear membranes. (2) Their formation inevitably precedes cell death. (3) Contain virus-specific glycoproteins. (4) Contain cellular lipids. (5) Only occur in viruses with helical nucleocapsids. 2. Indicate whether TRUE or FALSE. Enzyme immunoassays for anti-viral antibodies: (6) Require living cells and virus. (7) Can be completed in a few minutes. (8) May be used to detect antibodies to internal viral antigens. (9) Must be conducted under sterile conditions. (10) Are suitable for use in practice laboratories. 3. Indicate whether the following statements about coronaviruses are TRUE or FALSE. (11) Coronaviruses are enveloped RNA-containing viruses. (12) All of the porcine coronaviruses are antigenically very closely related. (13) Feline coronaviruses are readily isolated in cell culture. (14) Feline infectious peritonitis is due to cardiomyopathy. (15) Infectious bronchitis virus causes egg drop in adult birds. 4. Indicate whether the following statements about herpesviruses are TRUE or FALSE. (16) Herpesviruses obtain an envelope from the nuclear membrane. (17) Alphaherpesviruses do not grow well in cell culture. (18) Herpesviruses are antigenically stable. (19) In latency, herpesvirus DNA is integrated into cellular DNA. (20) Some herpesviruses can cause tumours. 14 Division of Infection & Immunity 5. Indicate whether the following statements about rotaviruses are TRUE or FALSE. (21) Non-enveloped and contain double-stranded RNA. (22) Have a segmented genome. (23) Are transmitted by arthropod vectors. (24) Produce diarrhoea in animals during the first week of life. (25) Only replicate in mitotically active cells within the intestinal crypts. 6. Indicate whether the following statements about influenza A viruses are TRUE or FALSE. (26) Enveloped RNA viruses with a segmented genome. (27) Envelope contains separate glycoproteins with neuraminidase and haemagglutinating properties. (28) Cell mediated immunity to influenza virus may be directed against internal proteins of the virus. (29) Pandemics of influenza virus are often associated with antigenic change in the nucleocapsid protein. (30) Neutralising antibody is largely directed at the haemagglutinin glycoprotein. 7. Indicate whether the following statements about African swine fever virus (ASFV) are TRUE or FALSE. (31) ASFV replicates in the cytoplasm of infected cells (32) ASFV is transmitted from wild to domestic pigs by a soft tick vector (33) ASFV does not cause disease in wild pigs in Africa (34) ASFV could not spread between domestic pigs in the UK because there are no suitable arthropod vectors (35) ASFV does not induce virus neutralising antibodies 8. Indicate whether each of the following statements about inactivated viral vaccines is TRUE or FALSE. (36) May only require a single dose. (37) Require an adjuvant for efficacy. (38) Induce mucosal immunity. (39) More likely than live vaccines to be inactivated by maternally derived antibodies. (40) Readily induce cytotoxic T cell responses. 9. Indicate whether the following statement about bovine spongiform encephalopathy (BSE) are TRUE or FALSE (41) Caused by natural transmission of the scrapie agent between sheep and cattle. (42) Incidence has increased slowly over a period of 20 years. (43) More common in dairy cattle than in beef cattle. (44) Is declining in incidence. (45) Specifically diagnosed by an enzyme immunoassay for anti-prion antibodies. 10. Indicate whether the following statements about immunofluorescence (IF) are TRUE or FALSE: (46) IF can identify the site of virus antigen production in infected cells. (47) IF cannot be used to measure levels of anti-viral antibodies in serum. (48) Direct IF is much more sensitive than indirect IF. (49) Intracellular viral antigens can be identified in live cells by IF. (50) IF is routinely used in practice laboratories. 15 Course Information 11. Indicate whether TRUE or FALSE. In the isolation of Campylobacter from a sample of faeces: (51) A selective medium containing antimicrobials is essential to suppress other elements of the flora. (52) Anaerobic cultures are essential. (53) Campylobacters can be identified presumptively by their morphology in Gramstained smears. (54) Identification can be confirmed biochemically. (55) The presence of acid-fast bacteria is diagnostic. 12. Indicate whether TRUE or FALSE. The genome (chromosome) of bacteria is: (56) Single stranded DNA. (57) Double stranded DNA. (58) Linear and may be either DNA or RNA. (59) Circular and may be either DNA or RNA. (60) Bounded by a nuclear membrane. 13. Indicate whether TRUE or FALSE. The outer membrane of Gram-negative bacteria: (61) Contains lipopolysaccharide. (62) Contains peptidoglycan. (63) Acts to exclude large molecules from direct contact with the bacterial cell. (64) Acts as a "molecular sponge". (65) Can be responsible for resistance to some antibiotics. 14. Indicate whether TRUE or FALSE. (66) Essential cell functions. (67) Antibiotic resistance. (68) Self-transmissibility. (69) Motility. (70) Pathogenic determinants. 15. Indicate whether TRUE or FALSE. (71) Macroconidia are sexual spores. (72) Arthrospores may remain viable in the environment. (73) Aseptate mycelium is produced by the Phycomycete class of fungi. (74) Dermatophytes are keratolytic. (75) Dimorphic fungi have two forms: yeast and mycelial. 16. Indicate whether TRUE or FALSE. MacConkey agar: (76) Escherichia coli (77) Klebsiella pneumoniae (78) Bordetella bronchiseptica (79) Salmonella typhimurium (80) Pseudomonas aeruginosa 16 Plasmids may encode: The following bacteria are lactose fermenters on Division of Infection & Immunity 17. Indicate whether TRUE or FALSE. and typing of Salmonella: (81) Phage typing. (82) Plasmid profiling. (83) API enteric strips. (84) Lancefield grouping. (85) Serotyping The following tests are used in the identification 18. Indicate whether TRUE or FALSE. Dichelobacter nodosus is: (86) A strict anaerobe. (87) Piliated. (88) Belongs to a single antigenic type. (89) Produces a powerful exotoxin. (90) Keratolytic 19. Indicate whether TRUE or FALSE. (91) Anthrax is associated with sewer workers. (92) Leptospira icterohaemorrhagiae is an increasing problem in dairymen. (93) Orf is a Prescribed Disease under the Industrial Injuries Scheme. (94) The COSHH Regulations only apply to non-infectious hazards. (95) Chlamydia from enzootic abortion in ewes frequently causes human illness. 20. Indicate whether TRUE or FALSE. (96) Toxocariasis is a more common cause of human infection than is toxoplasmosis. (97) Treatment with tetracyclines for 25 days will eliminate the carriage of Chlamydia psittaci in infected parrots. (98) Rabies in bats in Europe is being reduced by wildlife vaccination. (99) Leptospira canicola infection in people is increasing. (100) Microsporum canis infection is the most common zoonotic cause of ringworm in people. 17 Course Information ANSWERS TO MULTIPLE CHOICE QUESTIONS EDPAC A A D 1 22 43 64 B B A 2 23 44 65 B E B 3 24 45 66 67 E E C 4 25 46 68 D B D 5 26 47 69 B A B 6 27 48 70 71 E D D 7 28 49 72 A B E 8 29 50 73 C A B 9 30 51 74 10 11 12 B C E 31 32 33 A D B 52 53 54 D B A 13 14 15 A C D 34 35 36 A D B 55 56 57 B E D 16 17 18 19 20 21 D B A D A B 37 38 39 40 41 42 B A D D B E 58 59 60 61 62 63 A B A A B C C C B A C C E D C A C TRUE/FALSE T 13 61 F 62 T 63 T 64 T 65 1 1 2 3 4 5 F F T T F 5 21 22 23 24 25 T T F T F 9 41 42 43 44 45 F F T T F 2 6 7 8 9 10 F T T F T 6 26 27 28 29 30 T T T F T 10 46 47 48 49 50 T F F F F 14 66 67 68 69 70 3 11 12 13 14 15 T F F F T 7 31 32 33 34 35 T T T F T 11 51 52 53 54 55 T F T T F 15 4 16 17 18 19 20 T F T F T 8 36 37 38 39 40 F T F F F 12 56 57 58 59 60 F T F F F 16 18 17 81 82 83 84 85 T T T F T F T T F T 18 86 87 88 89 90 T T F F T 71 72 73 74 75 F T T T T 19 91 92 93 94 95 F F T F F 76 77 78 79 80 T T F F F 20 96 97 98 99 100 F F F F T Division of Infection & Immunity ESSENTIAL COMPETENCES REQUIRED OF THE VETERINARY SURGEON INTRODUCTION 1. An important statutory function of RCVS is to determine the “standard of proficiency” and “knowledge and skill” to fit veterinary surgeons for practising in the United Kingdom. The standard set for registration is a key benchmark that affects the approval of veterinary degree courses in the UK and overseas, and should also be seen as the starting point for consideration of an individual’s continuing education and training needs after registration. Maintaining and further developing professional competence is a requirement for all veterinary surgeons. 2. This document sets out the essential competences required of the veterinary surgeon for membership of the Royal College of Veterinary Surgeons. 3. There are many definitions of ‘competence’ and many views on how it can be developed and assessed. In general terms, however, competence is a concept that integrates knowledge, skills and attitudes, the application of which enables the professional to perform effectively, and to respond to contingencies, change, and the unexpected. 4. This document takes a broad definition of competence as being “the ability to perform the roles and tasks required by one’s job to the expected standard” (Eraut & Boulay, 20001). The definition recognises that requirements and expectations change depending on the job role and context. It also recognises that competence develops, and that an individual may work ‘competently’ at many different levels, either at different stages of their career, or indeed from one day to the next depending on the nature of their work. 5. Eraut and Boulay (2000) point out the importance of distinguishing between an individual’s competence – what one can do, and performance – what one actually does. RCVS is concerned with competence when it undertakes its primary function of determining “the standard of proficiency required for registration” and ensuring that individuals “will have acquired the knowledge and skill needed for the efficient practice of veterinary surgery” (Veterinary Surgeons Act 1966, section 3). Performance may be affected by factors such as workload, working conditions, levels of support, and so on. The regulation of performance is a function of RCVS through its statutory disciplinary powers. 6. The essential competences have been broken down into three main areas. They are: - - A General professional competences and attributes describing the distinguishing characteristics of a veterinary surgeon B Underpinning knowledge and understanding describing in general terms the breadth of knowledge and understanding needed for a career as a veterinary surgeon, and for subsequent professional development in whatever sphere of veterinary science the individual wishes to pursue C Practically-based veterinary competences 1 “Developing the Attributes of Medical Professional Judgement and Competence”, a review funded by the Postregistration Medical and Dental Education Research Initiative of the Dept. of Health’s Policy Research Programme, by Professor Michael Eraut and Benedict du Boulay, University of Sussex. The full paper is available on the web at http:/www.cogs.susx.ac.uk/users/bend/doh 19 Course Information describing the basic practical competences that are expected at the point of graduation. The ten guiding principles of the RCVS Guide to Professional Conduct should be seen as overarching requirements for registration (and by implication, continued registration) with the College. The Guide states that: Your clients are entitled to expect that you will: 1. make animal welfare your first consideration in seeking to provide the most appropriate attention for animals committed to your care 2. ensure that all animals under your care are treated humanely and with respect 3. maintain and continue to develop your professional knowledge and skills 4. foster and maintain a good relationship with your clients, earning their trust, respecting their views and protecting client confidentiality 5. uphold the good reputation of the veterinary profession 6. ensure the integrity of veterinary certification 7. foster and endeavour to maintain good relationships with your professional colleagues 8. understand and comply with your legal obligations in relation to the prescription, safekeeping and supply of veterinary medicinal products 9. familiarise yourself with and observe the relevant legislation in relation to veterinary surgeons as individual members of the profession, employers, employees and business owners 10. respond promptly, fully and courteously to complaints and criticism. ESSENTIAL COMPETENCES REQUIRED OF THE NEW VETERINARY GRADUATE “DAY ONE SKILLS” A1 - GENERAL PROFESSIONAL SKILLS AND ATTRIBUTES The new veterinary graduate should be able to: A1.1 Communicate effectively with clients, the lay public, professional colleagues and responsible authorities; listen effectively and respond sympathetically to clients and others, using language in a form appropriate to the audience and the context A1.2 Prepare clear case reports and maintain patient records in a form satisfactory to colleagues and understandable by the public A1.3 Work effectively as a member of a multi-disciplinary team in the delivery of services to clients A1.4 Be aware of the ethical responsibilities of the veterinary surgeon in relation to individual patient care and client relations, and also more generally in the community in relation to their possible impact on the environment and society as a whole A1.5 Be aware of the economic and emotional climate in which the veterinary surgeon operates, and respond appropriately to the influence of such pressures A1.6 Be willing to use one’s professional capabilities to contribute as far as possible to the advancement of veterinary knowledge in order to benefit veterinary practice and further improve the quality of animal care and public health A1.7 Have an elementary knowledge of the organisation and management of a veterinary practice, including: awareness of own and employer’s responsibilities in relation to employment and health and safety legislation, and the position relating to lay staff and public liability 20 Division of Infection & Immunity awareness of how fees are calculated and invoices drawn up, and the importance of following the practice’s systems for record keeping and book-keeping, including computer records and case reports ability to use information technology effectively to communicate, share, collect, manipulate and analyse information importance of complying with professional standards and policies of the practice A1.8 Understand the need and professional obligation for a commitment to continuing education and training, and professional development, throughout one’s professional life A1.9 Conduct oneself in a professional manner with regard to the veterinary surgeon’s professional and legal responsibilities and understand and apply the ethical codes as set out in the RCVS Guide to Professional Conduct A1.10 Be able to cope with uncertainty and adapt to change A1.11 Develop a capacity for self-audit and willingness to participate in the peer-review process A1.12 Be aware of personal limitations, and demonstrate awareness of when and from where to seek professional advice, assistance and support. (Commentary: This last item is considered to be one of the most important, and should guide all new veterinary graduates when undertaking their professional duties. Veterinary surgeons undertaking procedures on patients must at all stages in their careers be fully competent in their performance, or be under the close supervision of those so competent. When in doubt, the new veterinary graduate must seek professional support and in the interests of animal and human health, should not attempt to undertake complex procedures unsupervised.) B1 - UNDERPINNING KNOWLEDGE AND UNDERSTANDING The new veterinary graduate will need to have acquired a thorough knowledge and understanding of the following: B1.1 The sciences on which the activities of veterinary surgeons are based B1.2 Research methods and the contribution of basic and applied research to all aspects of veterinary science B1.3 How to evaluate evidence B1.4 The structure and functions of healthy animals, and all aspects of their husbandry B1.5 The aetiology, pathogenesis, clinical signs, diagnosis and treatment of the common diseases and disorders that occur in the common domestic species in the UK B1.6 Legislation relating to the welfare (including transport) of animals and notifiable diseases B1.7 Medicines legislation and guidelines on responsible use of medicines B1.8 The principles of disease prevention and the promotion of health and welfare B1.9 Veterinary public health issues including zoonoses. C1 - PRACTICAL COMPETENCES The new veterinary graduate should be able to undertake the following: C1.1 Obtain an accurate and relevant history of the individual animal or animal group, and its/their environment C1.2 Handle and restrain an animal safely and humanely, and instruct others in performing these techniques C1.3 Perform a complete clinical examination C1.4 Attend all species in an emergency and perform basic first aid (Commentary: problems to be handled for any species include first aid management of haemorrhage, wounds, breathing difficulties, eye & ear injuries, unconsciousness, clinical deterioration, burns, tissue damage, internal organ damage and cardiac arrest. First aid to 21 Course Information be applied includes bandaging, cleaning, immobilising limbs, resuscitation procedures, haemorrhage control.) C1.5 Assess correctly the nutritional status of an animal and be able to advise the client on principles of husbandry and feeding (Commentary: this applies to commonly presented cases and would not, for example, be expected to include advanced nutritional advice for complex cases, eg. high performance horses, high yielding diary cows, certain exotic or zoological species.) C1.6 Collect, preserve and transport samples, perform standard laboratory tests, and interpret the results of those generated in-house, as well as those generated by other laboratories (Commentary: new graduates are expected to have a working knowledge of tests to be undertaken include conditions relating to infectious & contagious diseases; alimentary system; respiratory system; circulatory system; urinary system; nervous system; endocrine system; mucucutaneous system; musculoskeletal system; trauma; poisoning; obstetrics; paediatrics; parturition; reproduction) C1.7 Use radiographic, ultrasonic, and other technical equipment which can be used as a diagnostic aid, safely and in accordance with current regulations C1.8 Follow correct procedures after diagnosing notifiable, reportable and zoonotic diseases C1.9 Know and apply the RCVS twelve Principles of Certification correctly C1.10 Access the appropriate sources of data on licensed medicines; prescribe and dispense medicines correctly and responsibly in accordance with relevant legislation and ensure that medicines and waste are safely stored and/or disposed of C1.11 Correctly apply principles of sterilisation of surgical equipment C1.12 Correctly apply principles of aseptic surgery C1.13 Safely perform sedation, general and regional anaesthesia, implement chemical methods of restraint, and assess and control pain C1.14 Advise on, and administer appropriate treatment (Commentary: the new veterinary surgeon must always seek professional advice and support if presented with a case beyond his or her immediate capability – see item A.12) C1.15 Recognise when euthanasia is necessary and perform it humanely, using an appropriate method, whilst showing sensitivity to the feelings of owners and others, and with due regard to the safety of those present; advise on disposal of the carcase C1.16 Perform a basic gross post mortem examination, record details, sample tissues, store and transport them C1.17 Perform ante mortem inspection of animals destined for the food chain and correctly identify conditions affecting the quality and safety of products of animal origin C1.18 Assess and implement basic health and welfare records (and production records where appropriate) C1.19 Advise on, and carry out preventive and prophylactic programmes appropriate to the species and commensurate with accepted animal health, welfare and public health standards, seeking advice and assistance where necessary from professional colleagues C1.20 Minimise the risks of contamination, cross infection and accumulation of pathogens in the veterinary premises and in the field. 22