Ann Jing Wen Tan ajtan@ucdavis.edu 2950 Portage Bay West Davis CA 95616 (408) 775-9934 Education Majors in Soil and Water Science (BS) and Evolution, Ecology and Biodiversity (BS) UC Davis, Expected Graduation Date December 2010 GPA 3.94 Research Experience Student Assistant – Department of Viticulture and Enology, UC Davis May 2008-October 2008 Involved in the first phase of a three year project that quantified soil spatial variability and variation in water usage with grape vines planted in two contrasting soil types within the same vineyard block, throughout the growing season. Performed detailed vineyard soil surveys, which includes soil sampling, description, determination of color and texture, soil pH and electrical conductivity measurements. Helped installed suction lysimeters to obtain soil solution samples and sampled and prepared grape vine tissue (leaves and petioles) for analysis. Monitored micro-meteorological instruments that have been installed in the vineyards. Under the Kearney Foundation Fellowship, I wrote a report and presentation based on my experience. Student – Bodega Marine Laboratory, UC Davis June 2009-July 2009 Learned basic research techniques like randomization, and familiarized with basic equipment such as a hemocytometer and tissue grinder Came up and designed an experiment that tested the effects of acidity on sea anemones Presented to the lab and published my findings to the laboratory’s library. Undergraduate Researcher – Scow Soil Microbial Ecology Lab, UC Davis April 2009-Present Learned and wrote standard operating procedures for research techniques dealing with microorganisms, such as microscopy, culturing and maintaining algae, and measurements of growth, lipid and selenium content. Researched effects of different growth conditions on the lipid content and growth rate of algae. Wrote a thesis and presented my research for the UC Davis Undergraduate Research Conference Work Experience Student Assistant – Foundation of Plant Services February 2009-June 2009 Tended young grapevines by pruning and watering them Propagated grapes and roses Labeled and organized plants YOMA! Tutoring June 2007-September 2007 Taught English to students in middle school who has English as a second language Came up with lesson plans Graded their writing assignments Abstracts and Presentations Soil Related Differences in Plant Vigor and Evapotranspiration in Two Merlot Vineyards of the Sacramento-San Joaquin Delta, California, USA. Jean-Jacques Lambert, Ann Tan, Alessandro Morello, Robert A. Coates, Chuck Ingels, Paul Verdegaal, James A. Wolpert, Michael Delwiche and Richard L. Snyder. Submitted to the 16th International Symposium GiESCO (Groupe d‘Etude des Systèmes de Conduite de la Vigne), held July 12-16, 2009, UC Davis. Exploration on Anthropogenic Effects on Algal Reponses to Acidification in Anthopleura xanthogrammica. Ann Tan, Presented at Bodega Marine Laboratory July 30 2009. Ocean acidification has been an increasing concern due to its effect on coral reefs. Many corals are becoming bleached as their obligate algal symbionts are being expelled due to stress. A closely related cousin to corals, one that also has algal symbionts, is the sea anemone Anthopleura xanthogrammica. However, the consequences of ocean acidification and protection of the sea anemone in marine reserves on the symbiotic relationship have not been explored. In this study, I exposed A. xanthogrammica to the pH conditions predicted for 2110 and tested the hypothesis that sea anemones from a protected reserve would retain their algal symbionts better than these from a publicly accessible state park, particularly when subjected to less trampling. I collected 12 anemones from Bodega Marine Reserve and 12 anemones from Campbell Cove, a state park, and separated them in half, placing six reserve anemones and six park anemones into two distinct tanks with a control pH and 2010 pH. To test for trampling effects, I pushed a rock on half the anemones in each treatment. Results are inconclusive, but suggestive that anemones initially responded to acidic conditions. After eight days however, all the anemones had approximately the same algal concentrations in their tissues. This suggests that A. xanthogrammica is resilient to acidic conditions and indicates that ongoing climate changes influences different groups of cnidarians (i.e., corals verses anemones) in different ways. Exploring Economically Sensible Solutions in Optimizing Lipid Growth and Lipid Content. Ann Tan, Presented at the UC Davis Undergraduate Research Conference May 1 2010. The depletion of fuel reserves is spurring a search for sustainable sources of alternative energy. One potential solution is to grow and harvest microalgae lipid for biofuel. I experimented with the growing conditions of three algal strains: two in the genus Chlorella, UTEX 2341 and UTEX 2805, and a Chlorophyta isolated from agricultural wastewater at the Red Rock Ranch in Five Points, California (RRR). The strains were grown under various conditions to determine which supported the highest biomass. UTEX 2341 and UTEX 2805 growth were compared in three different growth conditions: in a growth chamber, in a shaker, and with a stir bar. Then the effect of photoperiod on UTEX 2341 and RRR was measured. Optical density (OD) and chlorophyll A (ChA) were measured daily during the experiment and then algae were harvested to analyze for lipid content. In the first experiment, UTEX 2341 grew better under all growth conditions than did UTEX 2805, although within each species the results were mixed in terms of which growth condition was the best. In the photoperiod experiment, both strains grew best with exposure to constant light, followed by 16 hours, and did most poorly at 12 hours. The OD and the ChA measurements did not correspond to each other. UTEX 2341 had comparable lipid content at 16 and 24 hours, and substantially less with 12 hours of light. Academic Service President, Strategies for Ecology Education, Diversity and Sustainability (SEEDS), UC Davis Chapter Awards and Grants Outstanding Senior Award: Land, Air and Water Resources (2010) Soil and Water Science Departmental Citation for Outstanding Performance (2010) Kearney Foundation, Funded $3000 dollars (2008) Recipient, Regents Scholarship (September 2007- present) Completed three year of Integrated Studies Honors Program References Randy A. Dahlgren o Professor of Soil Science and Pedologist o radahlgren@ucdavis.edu o Voice: 530-752-2814 o Fax: 530-752-1552 Kate M. Scow o Professor of Soil Science and Microbial Ecology o kmscow@ucdavis.edu o Voice: 530-752-4632 o Fax: 530-752-1552 Brian Gaylord o Associate Professor of Evolution and Ecology o bpgaylord@ucdavis.edu o Voice: 707-875-1940 o Fax: 707-875-2009