Amgen Biotech Experience Materials Request Form
Each teacher at the participating school must complete and return this form for his/her
classes
Name of Teacher and Email address:___________________________________________________________
Name of School:____________________________ Your Phone:_______________School Phone:_________________
List other teachers doing the labs during this time block and their email addresses: ___________________________
________________________________________________________________________________________________
________________________________________________________________________________________________
Reservation/Pick-up Date:___________________ Equipment + Supplies ______ Supplies only ______
Select the Pathway your students will be doing and fill in the requested numbers
Complete Genetic Engineering Sequence (Labs 1, 2, 3, 4, 5, and 6)
Description: This laboratory sequence tells the “complete” biotechnology story from moving a gene of interest into an
engineered expression vector. Unlike the Abridged Genetic Engineering sequence, this sequence involves the digestion
of pARA and pKAN-R followed by a ligation lab to produce recombinant plasmids. This represents the most challenging
set of protocols. This sequence includes Purifying the Fluorescent Protein (Lab 6), but you may choose not to include this
lab.
Next to each class name please enter the number of classes doing this lab series.
______Number of students running this sequence
Intro Bio. ______ Advanced/Honors Bio. ______
______Number of groups running this sequence
AP Bio. ______ Biotech ______
Other (Name and number of classes)________________________
Lab 6 Yes_____ No_____ Date for Lab 5 _________
Abridged Genetic Engineering Sequence (Labs 1, 2a, 4a, 5a, and 6)
Description: This sequence omits the pARA/pKAN-R digestion and ligation of restriction fragments, and starts with the
rfp gene already ligated into the expression vector. Students run a plasmid digest and electrophoresis separating pARAR restriction fragments. This was written to shorten the labs required to get to the protein purification step and to
increase the likelihood of students succeeding in producing red transformants. The sequence requires less teacher
preparation time. This sequence includes Purifying the Fluorescent Protein (Lab 6), but you may choose not to include
this lab.
Next to each class name please enter the number of classes doing this lab series.
______Number of students running this sequence
Intro Bio. ______ Advanced/Honors Bio. ______
______Number of groups running this sequence
AP Bio. ______ Biotech. ______
Other (Name and number of classes)______________________
Lab 6 Yes ______ No ______ Date for Lab 5A ________
Focus on Bacteria Sequence (Labs 1, 5b, and 6)
Description: The focus of this sequence is on the importance of plasmids as vectors for use in genetic engineering and
the ability of bacteria to utilize the genetic information of the plasmid to produce a protein product. It is not only
important to produce the protein, it is equally important that the protein be capable of being collected and purified.
This sequence includes Purifying the Fluorescent Protein (Lab 6), but you may choose not to include this lab.
Next to each class name please enter the number of classes doing this lab series.
______Number of students running this sequence
Intro Bio. ______ Advanced/Honors Bio. ______
______Number of groups running this sequence
AP Bio. ______ Biotech ______
Other (Name and number of classes) ______________________
Lab 6 Yes ______ No ______ Date for Lab 5B _________
*SEE NEXT PAGE*
2/16/2016
ABE-LA CSU-CI Distribution Center
Introduction to Biotechnology (Lab 1)
Description: The focus of this lab is to enable the students to become proficient in basic biotechnology lab skills. It
includes using the micropipette and running a gel electrophoresis.
Next to each class name please enter the number of classes doing this lab series.
_______Number of students running this lab
Intro Bio. ______ Advanced/Honors Bio. ______
_______Number of groups running this lab
AP Bio. ______ Biotech ______
Other (Name and number of classes)______________________
Colony PCR (new 2015)
Description: The purpose of this lab is to confirm the cells producing red fluorescent protein have been transformed
with the plasmid carrying the rfp gene, pARA-R, using a fundamental molecular biology tool, PCR.
Next to each class name please enter the number of classes doing this lab series.
_____Number of students running this lab
Intro Bio____ Advanced/Honors Bio ______
_____Number of groups running this lab
AP Bio______ Biotech______
Other (Name and number of classes)_________________
Genomic DNA Extraction and PCR (last year for this)
Description: These labs are independent and are not considered part of the Pathways. If the teacher has attended an
Amgen Biotech Experience workshop, which included Genomic DNA Extraction and PCR training, he/she may choose to
include these labs with the Pathway sequence selected. The teacher may also choose to do these as an independent lab
experience.
Next to each class name please enter the number of classes doing this lab.
______Number of students running this sequence
Intro Bio. ______ Advanced/Honors Bio. ______
AP Bio. ______ Biotech ______
Other (Name and number of classes)_____________________
Important Notes:
Suspension Culture of Cells (Prep for Lab 6)
If your classes are doing Lab 6 Purifying the Fluorescent Protein, time to complete the lab can be challenging. The
second and third options below may save you and/or your students time. Please indicate below how you would like to
prepare/receive the Suspension Culture of Cells (Prep for Lab 6) by marking one of the options below.
______I want to culture the transformed cells in the shaking incubator at my school as part of Lab 6.
______Please culture the cells at the distribution center and send the unlysed cells. My classes will do the Lab 6 cell lysis
and chromatography.
______ Please culture the cells at the distribution center and send the prepared supernatant ready for the Lab 6
chromatography.
The Student Guides and Teacher’s Guide (new editions 2015) can be downloaded from the “Curriculum” page at
www.amgenbiotechexperience.com. Be sure to download the correct sequence, if not downloading the entire
guides.
Would you prefer to borrow Student Guides? If yes, how many? ___________
Do you have an incubator at your school? Yes ________ No ________
If you are requesting “Supplies Only”, do you need any additional equipment (e.g., extra pipets, water bath, etc.)?
*****RETURN THIS FORM TO YOUR SITE COORDINATOR AT LEAST ONE MONTH BEFORE YOUR RESERVATION DATE
2/16/2016
ABE-LA CSU-CI Distribution Center