Control/Tracking Number : 04-A-1087-ESHG
Activity :Abstract-European Human Genetics Conference
Current Date/Time : 2/22/2004 7:12:09 AM
Free manipulation and overstretching of genes for direct nanoscopic sequencing of DNA
A. A. Kalachev1, N. Severin2, J. Barner2, J. P. Rabe2;
1
Plasmachem GmbH, Mainz, Germany, 2Humboldt University of Berlin, Berlin, Germany.
The direct analysis of single macromolecular chains at the level of their primary chemical structure like the
nucleotide sequence in DNA, represents one of the current challenges in macromolecular and life sciences. With
respect to DNA, the method of direct sequencing would open a new opportunity for gene mapping of humans.
Different nanoscopic methods are approaching the resolution level required for direct recognition of single
bases: scanning tunneling microscopy (STM), tip enhanced Raman spectroscopy (TERS) and their different
modifications. However, much less progress achieved in respect to proper arranging of single DNA-chains on
solid substrates to prepare polynucleotides for direct non-destructive high speed analysis. To elaborate proper
molecular pattern one need first to prepare a macromolecular array of single DNA molecules, where the different
single polymer chains from the sample are first properly positioned with respect to each other on the surface and
then stretched and optionally over-stretched, followed by reading of the structure by some nanoscopic multiarrayed analyzer.
Moreover, resulted “Addressed Molecular Array DNA-Chip” should be finally improved to correct “Molecular
lithography” defects (strong deviations from position and linearity).
We report for the first time on new method of the free manipulation (free 2D-shaping) of already deposited
(physisorbed) single chosen ss- and ds-DNA-chains on a substrate. Method allows to move chains as a whole, to
stretch it, to remove stretching defects and to overstretch ds-DNA helix into two parallel fixed single strands.
Method is universal and can be applied for other classes of synthetic and natural polymers.
Topic (Complete): None selected
Keywords (Complete): DNA-chip ; TERS ; Raman
Presentation Preference and Awards (Complete):
Presentation Preference : Oral preferred
Status: Complete
Vienna Medical Academy
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