Growth in plants Teaching Notes Introduction and context The animations in this series illustrate a range of processes that take place in plants. The animation can be used to observe movement and changes. The technical notes provided as a separate download explain how to navigate the various parts of the screen, whilst the teaching notes provide information and links to help explain the various processes. The animations in this series are: Transport of water and sugar in plants Respiration and photosynthesis Cell growth and division The purpose of these animations is to illustrate how the different processes going on inside plant are dependent on each other, and to show how different structures support these processes. Teaching Notes Scene Points to note Growth points in a plant You Tube video Increasing length – growth at the root tip You Tube video Unlike most animals, plants have specific zones where growth can occur, as shown by the circles on this screen. Each zone contains a meristem, which is a group of dividing cells, clusters of new cells which are increasing in size (elongation), and slightly older cells which become specialized into different tissues (differentiation). This animation looks in more detail at two of these zones – in the root tip and in the stem. Growth at the root tip results in the root being pushed further into the soil. To avoid abrasion the root tip is protected by a cap, which also needs a constant supply of new cells. Growth in the root is coordinated to ensure that root hairs for water absorption are only formed once elongation is complete, and to coincide with the formation of new xylem. More details about water uptake and transport can be seen in the animation ‘Transport of water and sugars in plants’. Science & Plants for Schools: www.saps.org.uk Animations – Growth in Plants – teachers’ notes: p. 1 The cells in the zone of division are small and unspecialized. They divide by mitosis, forming two identical nuclei, before the cytoplasm divides between the nuclei and a new cell wall is formed. The cell then undergoes a period of growth before dividing once more. Growth caused by cell division at root tip You Tube video Mitosis in a root cell You Tube video Increasing diameter – growth in vascular bundles You Tube video As a flat two dimension animation it is difficult to appreciate the complexity of this process. New cells are formed in all directions to create an elongating cylinder. The animation does not show how new cells in one direction form root cap cells, but form xylem and other root tissues in the other direction. The final scene in the root tip sequence illustrates how chromosomes behave during mitosis. Each chromosome consists of a pair of chromatids. These are pulled apart during anaphase by microtubules within the cell. The separate chromatids are then replicated to form new chromosomes, ensuring that each new cell formed is genetically identical to every other cell within the plant. The names of the different phases of mitosis are shown in the animation. There is also a photograph to show the appearance of onion cells undergoing different phases of mitosis for comparison. In the photograph the DNA of the chromosomes is artificially stained dark red. Increasing the diameter of the stem enables the plant to grow taller. Patterns of growth are complicated as additional height needs additional xylem to provide support and extra water transporting capacity. New phloem is also needed to replace older damaged tissue. The dividing cells are located inside the vascular bundle in meristems called cambium. The animation shows how increasing diameter of the stem is brought about by an increase in the amount of xylem, and new phloem being pushed towards the edge of the stem. In trees, cambium forms a continuous ring and as growth continues, compressed xylem in the centre of the stem becomes wood, and the phloem is protected by bark. Science & Plants for Schools: www.saps.org.uk Animations – Growth in Plants – teachers’ notes: p. 2 Growth caused by cell division in cambium You Tube video The initial scene shows the arrangement of cells around the cambium, with mature cells towards the top and bottom of the picture and youngest cells in the centre. For simplicity the animation then just focuses on one single column of cells. Similar changes to those shown in the animation take place at the same time throughout each vascular bundle. Small undifferentiated cells in the cambium divide and are pushed outwards. They enlarge, by absorbing water driven by osmosis, and start to differentiate. Xylem cells become strengthened with lignin and lose their cell contents. Phloem cells develop a large vacuole and many phloem cells lose their nucleus. Questions 1. List some possible ways of measuring the growth rate of a plant. Describe the advantages and some of the problems associated with each method. (Describe) Possible answers may include: Height – simple to carry out but ignores lateral growth, in seedlings grown in poor light an increase in height can indicate etiolation rather than growth Dry mass – gives accurate results of mass of plant tissue but only useful when sampling a large number of plants as each measurement destroys the plant Wet mass – Results vary given different water content of the plant at different periods off the day. An increase in wet mass does not imply an increase in the mass of plant tissue. Girth – diameter of stem – easy to measure and may be useful for trees but not other plants. Varies depending on transpiration rate. Problems taking repeat measurements in finding the same point to take the measurement. Size of roots or number of roots – interferes with further growth and so cannot be repeated using the same plant 2. How does the root tip get the raw materials needed to create new cells? (Explain) Possible answers may include: There is no phloem to deliver sugars and other raw materials to the root tip. These materials will need to pass through cortex cells, probably using carrier molecules and ATP to move to the root tip. Water needed for cell expansion and other processes would then enter the dividing cells by osmosis. 3. Use the information in these notes to explain why plants need specialized growth regions (meristems) unlike animals that have tissues each capable of creating new cells for growth or repair. (Analyse) Possible answers may include: Science & Plants for Schools: www.saps.org.uk Animations – Growth in Plants – teachers’ notes: p. 3 Once plants cells divide to form new cells those cells become specialized (differentiated). Some of those cell types then become too specialized to enable them to divide themselves. An example is xylem that loses its cell contents when mature and therefore is incapable of division and growth. The meristems can coordinate growth more easily than if cells of each tissue were able to divide independently. This ensures in the vascular cambium that xylem and phloem are both produced in a coordinated way, or in the root tip, the production of root cap cells is balanced against the production of new root cortex cells. This is necessary, as plants do not contain a central coordinating system. Plants are immobile and regulating growth from meristems allows plasticity in growth, responses to environmental stimuli and regrowth after herbivory. Animals can usually move away from stressful conditions, plants have to adapt growth via changing the balance of root and shoot meristem activity. 4. Compare and contrast the growth of sunflower plants with that of humans. (Link) Possible answers may include: Both organisms need to grow, to reach maturity so that they can reproduce. They both depend on an external source of raw materials to grow, and much growth is concerned with increasing their access to these raw materials. Their growth involves an increase in the number of cells, rather than the size of their cells increasing. This involves complex control mechanisms to ensure that growth of different parts is coordinated. Hormones are involved in this coordination. Plant growth occurs in discrete zones unlike humans where the majority of tissues are capable of dividing and forming new cells for that particular tissue. As more is learned about stem cells it appears that just like meristems in plants, in human cells may also in some situations be able to differentiate into different tissues. At a cell level there may be fewer differences between plant and human growth than was once believed. The overall growth of humans is determinate – there is a finite size beyond which growth stops. Growth proceeds in a series of phases under hormonal control. In sunflowers growth is indeterminate, allowing plants grown in favourable conditions to continue to grow to considerable height. References and further reading For teachers A large number of resources and activities available for teachers on the National STEM eLibrary http://www.nationalstemcentre.org.uk/elibrary/search?term=plant+growth&order=score For students Ideas for measuring plant growth http://www.sciencebuddies.org/science-fairprojects/project_ideas/PlantBio_measuring_growth.shtml Plant meristems – for younger students Science & Plants for Schools: www.saps.org.uk Animations – Growth in Plants – teachers’ notes: p. 4 http://www.bbc.co.uk/schools/gcsebitesize/science/add_ocr_pre_2011/growth_developm ent/singlecellrev2.shtml Acknowledgements Storyboard: Richard Needham CSciTeach Animation: Elektra Media Ltd With thanks to Prof Howard Griffiths, Department of Plant Sciences, University of Cambridge Science & Plants for Schools: www.saps.org.uk Animations – Growth in Plants – teachers’ notes: p. 5