Blitz Interaction Analysis
Bio-Layer Interferometry (BLI) is a label-free, biosensor technology that enables the real-time measurement of
molecular interactions.
A typical kinetic interaction can be measured over the range of 1 mM to 5 pM.
Machine sensitivity is 0.1 times Kd and the saturation is 100 times the Kd.
Starting ligand volume and concentration 4 µL 50µg/ml.
Starting analyte volume and concentration 4 µL up to 10µM.
Dip and Read™ Biosensors
available at the facility.
ProA - Protein A
Binds with high affinity to the Fc region of human IgGs. Binds with moderate affinity to many subtypes of
mouse and rabbit IgG. Applications include quantitation of IgG in cell line development, clone selection,
process optimization, and production monitoring
ProG - Protein G
Binds with high affinity to the Fc region of murine, rat, goat, and bovine IgGs. Binds with moderate affinity to
many subtypes of human IgG. Applications include quantitation of IgG in cell line development, clone
selection, process optimization, and production monitoring.
SA – Streptavidin
Streptavidin-coated biosensor with high binding capacity. Immobilizes biotinylated antibodies, proteins, and
nucleic acids to form a stable surface. Quantitation applications include antibody and protein quantitation in cell
line development, clone selection, process optimization, and production monitoring. Kinetic applications
include protein and antibody kinetic screening, affinity characterization (ka, kd, KD), epitope binning, and hit
validation.
NTA - Ni-NTA
Taking advantage of the wide-spread use of poly-histidine protein tags (HIS-tag) in the bio-pharmaceutical
industry, ForteBio's new Ni-NTA Biosensor further facilitates easy detection and rapid characterization of HIStagged molecules. Pre-immobilized with nickel-charged tris-nitriloacetic acid (Tris-NTA) from QIAGEN, the
Ni-NTA Biosensor enables direct quantitation of HIS-tagged proteins and their easy capture for subsequent
kinetic analyses with binding partners.
Project Description
Starting Ligand Material
Protein
Nucleic Acid
Antibody
Ligand Tag
Biotin
Histag
Analyte
Protein
Nucleic Acid
Buffer:
Contact
Name :
Team :
Email :
Protein Expression FacilityInstitut de Microbiologie de la Méditerranée FR 347931, Chemin Joseph Aiguier
Marseille 13009FranceTel: 04-91-16-45-64Fax: 04-91-71-78-96byrne@imm.cnrs.fr