CURRICULUM VITAE
OF
PROF. SHEIKH RIAZUDDIN
1
TABLE OF CONTENT
1.
2.
PROFILE OF DR. S. RIAZUDDIN.............................................................
3-5
BIOGRAPHICAL SKETCH ....................................................................... 06-13
3.
RECORD OF RESEARCH WORK
A. Scientific work...................................................................................... 15-23
B. Development work ............................................................................... 25-26
4.
LIST OF RESEARCH PAPERS PUBLISHED
A. National ............................................................................................... 27-30
B. International ......................................................................................... 31-50
List of Chapters in Books / Books written ..................................................
51
5.
LIST OF PATENTS ...................................................................................
52
6.
Suggested Referees .................................................................................
53
2
PROFILE
of
PROF. DR. S. RIAZUDDIN
Dr. S. Riazuddin obtained Ph.D from the University of Reading, England in
1970. Subsequently, he worked as Postdoctoral Fellow in some of the best labs
including those of Bryn Bridges at University of Sussex, Brighton, England; Larry
Grossman at Johns Hopkins University, Baltimore, USA; Tomas Lindahl at
Karolinska Institute, Stockholm, Sweden; and Eugene Nester/ Milton P. Gordon
at University of Washington, Seattle, USA. He returned to Pakistan in 1978, and
established a solid and definitive programme of molecular biology research first
at the Nuclear Institute for Agriculture and Biology, Faisalabad and then as
Founding Director, Centre of Excellence in Molecular Biology, University of the
Punjab, Lahore. To overcome the inherent constraints of practicing advanced
molecular biology research in a scientifically developing country, he established
collaborations with counterpart labs to combine the complementary strengths of
the participating laboratories. He made good uses of the local genetic resource in
studies on (i) restriction enzymes with Richard J. Roberts (Nobel Laureate), then
at Cold Sprig Harbor Labs, NY, USA; (ii) in hearing impairment with Thomas
Friedman at NIDCD, Bethesda, MD, USA; (iii) in speech impairment with Dennis
Drayna at NIH, MD, USA; and (iv) in vision impairment with Paul Sieving at NEI,
Bethesda, MD, USA and John Gottsch at Wilmer Ophthalmology Institute,
Baltimore, USA. He possesses the unique quality of combining the
complimentary strengths of participating laboratories resulting in extremely
productive collaborations. This has helped him to overcome the inherent
constraints of working in scientifically developing country and also been a source
of additional funding from agencies such as Rockefeller Foundation, NY USA;
National Science Foundation, Washington, USA; National Institute of Health,
Bethesda, USA and Commission of the European Community, Brussels,
Belgium.
Dr. Riazuddin is a superb experimentalist, extremely meticulous and
possesses a vision to foresee the breakthrough researches in molecular biology,
well in-time to assume leadership in new and emerging areas. In the early
1970’s, he recognized the potential of Nobel Prize discoveries by Ham Smith and
David Baltimore and immediately moved himself into DNA enzymology and
together with Richard J. Roberts (Nobel Laureate), he discovered 45 new
restriction enzymes. Recognizing Pakistan’s need in the agriculture sector, he
3
moved into plant molecular biology research and in collaboration with Eugene
Nester, Milton P. Gordon, and Marc van Montagu, he genetically engineered rice
and cotton with Bt genes. He foresaw the revolutionary potential of the
completion of human genome sequence in 2000 and birth of accompanying
technologies and moved into research in genetic diseases. In collaboration with
Prof. Cao’s laboratory at Cagliari, Italy and Dr. Edward Wilcox’s laboratory at
NIDCD, Bethesda, USA, he made exemplary progress in identifying new genes
and loci through homozygocity mapping. As the field of DNA sequencing
advanced and high throughput (second generation) sequencing became
available, he changed the focus of his research from gene identification to gene
function and made significant contributions in elucidating the molecular pathway
involved in hearing, vision and speech processes. He developed more effective
methodologies which will play the role of key technologies in the next decade, in
studying molecular events involved in cellular pathways. Dr. Riazuddin’s
discoveries of a novel modifier gene (Riazuddin, et al., Nature, 2000), Acting
binding protein (Riazuddin et al., Am. J. Hum. Genet. 28: 137-43, 2006) and tight
junction proteins (Riazuddin et al., Am. J. Hum. Genet. 79: 1040-51, 2006) have
greatly contributing to the understanding of human hearing process. His
laboratory identified and characterized CDH23 and PCDH15 (Ahmed et al., Hum.
Genet. 124: 215-23, 2008) genes which are essential for both sound and vision
transduction. Identification of these genes has given a new insight into possible
therapy to overcome retinal degeneration and patients with Usher syndrome. His
recent studies on how active bundling protein TRIOBP forms resilient rootlets of
hair cells stereocilia essential for hearing (the stereocilia figures were featured on
the front page of Cell May 2010) and elucidation of biochemical parameters
leading to genetic susceptibility to stuttering (the New England Journal of
Medicine, Impact Factor 52) are indeed breakthrough discoveries
Several of Dr. Riazuddin’s research papers have attracted editorial
comments and reviews and also featured on the journal’s front page (please see
page-6). These works have opened new vistas and given new direction to
research in human hearing and speech developmental processes. His most
recent paper in American Journal of Human Genetics, has been selected for
highlighting in the next AJHG podcast.
A remarkable feature of Dr. Riazuddin’s studies on genetic diseases is the
nature of implicated genes such as Tricellulin, Tight Junction Proteins (DFNB28,
DFNB49) and other similar proteins (GNPT) that are expressed ubiquitously in all
human tissues, however, a pathological mutation would only the hearing or
4
speech process but no other pathway in the human body. The question
therefore, arises, why is it that the pathogenic mutation affects only the auditory/
speech function but no other function in the human body when the gene is
expressed ubiquitously. Dr. Riazuddin has proposed a hypothesis to explain this
phenomenon. This hypothesis states that efficiency of targeting the mutated/
affected genes is only partially reduced by the mutation that is critical for hearing
and speech but insignificant for other bodily functions. His present research is
focused on the testing of this hypothesis. His research group has successfully
generated knock-out, knock-in and conditional models of mouse and zebra fish to
study the expression of the mutated genes in different organs and at various
developmental stages. It is expected that in the next five years, the work will
generate exciting information on these issues.
Dr. Riazuddin’s research has been published in high impact factor journals
such as Cell, Nature Genetics, American Journal of Human Genetics and
New England Journal of Medicine. A number of his papers have been
selected for front page coverage, editorial reviews and comments and for
journal podcast coverage. The impact factor of his publications in 2010 is
148.183; during the last five years is 415.407 and cumulative impact factor
is 725.277.
Prof. Riazuddin has done equally well in extending the applications of his
research to the development of products and processes for public use in
agriculture and health sectors. Prof. Raizuddin’s group searched different
ecological environments to identify bacterial pesticidal genes (Bt genes) and
expressed them in cotton to breed insect resistant cotton varieties designated
CEMB-01 and CEMB-02. The newly developed Bt cotton varieties are in the
process of government approval. He further developed PCR-based diagnostic
procedures for the early and reliable detection of Hepatitis B and C virus. The
procedures are in use at CEMB as a general public facility since 2000. The most
important applied work that has come out of his lab is the cloning of several
human pharmaceutical proteins genes including interferon alfa, beta and gamma.
The generic human alfa-2a purified in his lab has been transformed into 3miu
injections and are waiting permission of ministry of Health to conduct human
clinical trials. The products and processes developed in his laboratory have been
patented in Pakistan/ USA.
5
BIOGRAPHICAL SKETCH
NAME:
SHEIKH RIAZUDDIN
BIRTH:
April 15, 1944, Lahore ,Pakistan
Citizenship:
USA (Green Card Holder)
Nationality:
Pakistani
Address (Permanent):
8210-Lyndhurst Street,LAUREL.MD20724-2927,USA
Address (Temporary):
10-P,Model Town Extension,Lahore-54700, PAKISTAN
Job Positions:
Visiting Professor, Seoul, National University Seoul S.
Korea (15% time).
Research Collaborator:
National Institute of Health,MD,USA (30% time )
Dean Post Graduate Studies/
Director Research,
Allama Iqbal Medical College Jinnah Hospital,
Allama Shabir Usmani, Road, Lahore-20, Pakistan
Tel: (92)-314-416-3348
Tel: (92)-42-3516-4422
(92)-42-3516-4933
EDUCATION
1954-59
SSC from Govt. High School, Lahore, Pakistan.
1959-65
HSC, B.Sc.(Hons.), M.Sc., Government College, Lahore.
1967-70
Ph.D. in Biochemistry, University of Reading, England.
SPECIALIZED COURSES / WORKSHOPS ATTENDED

A six-week course on plant molecular biology at Cold Spring Harbor Labs, NY, USA
(Summer 1982).

A workshop on “Biosafety-2 Advanced Research and Procedures: Case Studies for
Designated Experts” organized by ICGEB in Florence, Italy, April 3-8, 2000.

A workshop on “Holistic Foundations for Assessment and Regulation of Genetic
Engineering and Genetically Modified Organisms” organized by GENOK and UNEP
in Tromso, Norway, July 25 – August 07, 2004.
6
RESEARCH EXPERIENCE
1964-65
M.Sc. thesis on "Effect of heat on the digestibility of leaf protein
concentrates" with Dr. F.H. Shah at the PCSIR Laboratories, Lahore.
1965-67
Predoctoral research on "Isolation and identification of cholesterol
degradation products during photooxygenation of the steroid" with Dr.
S.H.M. Naqvi at the PAEC Centre, Lahore, Pakistan.
1967-70
Doctoral thesis on "Calcium metabolism in sheep during pregnancy and
lactation" with Prof. C. Tyler and Prof. R.F. Glascock, University of
Reading, England.
1970-71
Postdoctoral research on "Effect of radiation on bacterial mating" with
Prof. B.A. Bridges at the MRC Cell Mutation Research Unit, University of
Sussex, England.
1971-73
Studies on "Genetic restriction and modification in "H. influenzae" with Dr.
A. Muhammad, NIAB, Faisalabad, Pakistan.
1973-77
Postdoctoral research on "Repair of UV-irradiated bacterial DNA" in
Professor L. Grossman's laboratory at Brandies University, Waltham, MA/
Johns Hopkins University, Baltimore, MD, USA.
1977-78
Studies on the “mechanisms of repair of chemically modified DNA” in
Prof. T. Lindahl's laboratory at Karolinska Institute, Stockholm, Sweden.
1978-85
Studies on DNA enzymes and transgenic plants at the Nuclear Institute
for Agriculture and Biology, Faisalabad.
1988-89
Studies on transgenic plants at the Department of Biochemistry,
University of Washington, Seattle, WA, USA under Fullbright Fellowship
Programme.
1990-2004
Studies on transgenic plants at the Department of Biochemistry,
University of Washington, Seattle, WA USA during the summers every
year, as part of Biotechnology Career Fellowship, Rockefeller Foundation.
1985-2009
Teaching and research in Molecular Biology, National Centre of
Excellence in Molecular Biology, University of the Punjab, Lahore.
2010-todate
Dean Post Graduate Studies/Director Research, Allama Iqbal Medical
College, University of Health Sciences, Lahore.
EMPLOYMENT RECORD
1966-70
Scientific Officer, PAEC, Lahore, Pakistan.
1970-77
Senior Scientific Officer, Nuclear Institute for Agriculture & Biology,
Faisalabad, Pakistan.
1977-85
Principal Scientific Officer, Nuclear Institute for Agriculture & Biology,
Faisalabad, Pakistan.
7
1985-2002
Professor of Molecular Biology, University of the Punjab, Lahore,
Pakistan.
1988-1996
Visiting Professor, Department of Biochemistry, University of Washington,
Seattle, WA, USA [teaching for 3 months/ year].
2002-2005
Meritorious Professor of Molecular Biology, Univ. of the Punjab, Lahore.
2004-todate
Distinguished National Professor, Higher Education Commission/
National Centre of Excellence in Molecular Biology, Lahore, Pakistan.
1985-2009
Director, National Centre of Excellence in Molecular Biology, Lahore,
Pakistan.
2009-2010
Dean Post graduate Studies, Director Research, Allama Iqbal Medical
College/Jinnah Hospital, Lahore, Pakistan
2008-todate
Visiting Professor, Seoul National University, Seoul, South Korea.
HONOURS AND AWARDS
 Roll of honour from Govt. College, Lahore (1964).
 Roll of honour from Govt. College, Lahore (1965).
 Colombo Plan Doctoral Award (1967-71)
 IARC senior postdoctoral award (1977).
 Prix Isabelle De Caze De Nue , a Swiss Prize by the IARC, Lyon, France (1978).
 Gold Medal by the Pakistan Academy of Sciences to Biologists under 40 years of
age (1982).
 Tamgha-i-Imtiaz by the President of Pakistan (1984).
 Fullbright Fellowship (1987-88).
 Rockefeller Foundation's Biotechnology Career Fellowship (1989-2004).
 Gold Medal by Johns Hopkins Society of Scholars (1989).
 Sitara-i-Imtiaz by the President of Pakistan (1994).
 Carlos J. Finlay Prize for Microbiology by UNESCO (1997).
 Kharazmi International Prize by Iranian Government (1997)
 Izaz-e-Kamal by the President of Pakistan (1998).
 Hilal-i-Imtiaz by the President of Pakistan (2004).
 Gold Medal for the Distinguished Scientist of the Year by the Pakistan Academy of
Sciences (2004).
 COMSTECH Award 2005 in Biology (2005)
 Life Achievement Award (2005),by Higher Education Commission, Pakistan.
 Islamic Development award in Science and Technology (2008), Jeddah, Saudi
Arabia.
8
FELLOWSHIP OF ACADEMIES
 Fellow, Third World Academy of Sciences, Trieste, Italy (1993).
 Fellow, Pakistan Academy of Medical Sciences (1994).
 Fellow, National Academy of Medical Science (1995)
 Fellow, Pakistan Academy of Sciences (1997).
 Fellow, The Chemical Society of Pakistan (2003).
 Fellow, American Academy of Microbiology (2004).
MEMBER/FELLOW OF PROFESSIONAL SOCIETIES
 Member, Johns Hopkins Society of Scholars (1989).
 Member, American Phytopathological Society (2001).
 Member, American Society for Biochemistry and Molecular Biology (2001).
 Member, American Society for Microbiology (2001).
 Member, Pakistan Society of Biochemists (life Member).
 Member, Pakistan Society for Microbiology (Life Member).
 Member, Pakistan Society of Chemists (Life Member).
 Member, Human Genome Organization ‘HUGO’ (1997).
 Member, Asia-Pacific International Molecular Biology Network (IMBN) (2000).
PRINCIPAL INVESTIGATOR
A. Research Projects

“Enzymology of DNA repair” sponsored (1980-83) by US National Science
Foundation (USNSF) [7709501].

“Enzymology of DNA repair” sponsored (1982-84) by US National Science
Foundation (USNSF) [8005168].

“Search for New Restriction Endonucleases” sponsored (1983-86) by US National
Science Foundation (USNSF) [8216255].

Search for New Restriction Endonucleases” sponsored (1983-86) by US National
Science Foundation (USNSF) 8121869].

“Mutagenicity components of local herbs” sponsored (1980-85) by International
Agency for Research on Cancer (IARC), Lyon, France [DEC/80/001-85/003].

“Bt Genes in Chickpea” sponsored (1983-85) by US National Science Foundation
(INT 932008).

“Cloning of M. luteus repair genes in E. coli” sponsored (1987-90) by USNSF
(8713991).

“Levels of O6MeG in cancer susceptible tissues in local populations” sponsored
(1987) by IARC [C244].

“Host range specificity of Agrobacterium tumefaciens strains from Grown Gall
Tumors on Fruit Trees in Pakistan”, sponsored (1988-91) by Agency for International
Development, USA (Project I.D.8.275).

“Transfer of Bt genes to chickpeas to breed pod-borer resistance” sponsored (199294) by the Board on Science and Technology for International Development
(BOSTID), USA. [Project: CS-PB-1].
9

“Mechanism of Fungus Resistance in Chickpea” sponsored (1988-91) by the
Commission of the European Communities [# CI1* - 0847 – M].

“Expression of Insecticidal (Bt) Genes in Cotton Plants”, sponsored (1989-92) by the
Commission of the European Communities.

“Development of genetic resistance to common pests of Rice crop through
expression of Bt toxin genes” sponsored (1990-1993); by Rockefeller Foundation.
[RF89026, #44].

“Study of Expression of Bt toxin genes in Rice” sponsored (1993-1995); by
Rockefeller Foundation. [RF92003, Allocation #146].

“Development of genetic resistance to common pests of Rice crop through
expression of Bt toxin genes” sponsored (1993-96) by Rockefeller Foundation.
[RF93003, #188].

“Development of genetic resistance to common pests of Rice crop through
expression of Bt toxin genes” sponsored (1996-97) by Rockefeller Foundation.
[RF96001, #419].

“Development of genetic resistance to common pests of Rice crop through
expression of Bt toxin genes” sponsored (1997-2000) by Rockefeller Foundation.
[RF98001, #681].

“Study of Expression of Bt toxin genes in Rice” sponsored (2000-2002); by
Rockefeller Foundation. [RF93022, Allocation #228].

“Molecular Characterization and prenatal diagnosis for the prevention of Thalassemia in Pakistan” sponsored (1995-98) by International Atomic Energy
Agency (IAEA) [No.302-E1-PAK-8650]

“Identification of the Genetic Factors Responsible for the Variable Phenotype of
Thalassemia in Pakistan” sponsored (1998-2002) by International Atomic Energy
Agency (IAEA) [No. 302-E1-PAK-10507 and No.302-E1-PAK-10507.2].

“Molecular characterization of a new Bacillus thuringiensis Toxin genes” sponsored
(2000-03) by the International Centre for Genetic Engineering and Biotechnology
(ICGEB), Trieste, Italy [CRP/PAK97-03].

“Molecular and Genetic basis of Hereditary Deafness” sponsored (2003-06) by the
International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste,
Italy. [CRP/PAK02-01].

“Cellular Regeneration in the Ischemic Heart and other damaged organs” (2004-06),
University of Cincinnati & CEMB (Pak-US).

“Serum Interferon Response in HCV Hepatitis Patients” (2004-2006); University of
Colorado and CEMB (Pak-US).

“Intensification of Forensic Services and Research at Centre for Applied Molecular
Biology” (2004-06), Strand Analytical Laboratories, Indianapolis, and CEMB (PakUS).

Studies on the mechanisms of DNA repair and Cloning of Repair Genes sponsored
by US National Science Foundation (USNSF) (No. INT 8713965)

“Novel triple acting chimeric antimicrobials for eradication of multi-drug resistant
strains of Staphylococcus aureus” a Joint Research Project under Pak-US Science &
Technology Cooperative Programme 2007.
10

“Molecular and Genetic basis of Hereditary Deafness” sponsored (2009-11) by the
International Centre for Genetic Engineering and Biotechnology (ICGEB), Trieste,
Italy. [CRP/PAK08-11].

Genetic and Epigenetic Networks in Cognitive Dysfunction. Commission of the
European Community (GENCODYS,2010-2015)
B. Symposia/ workshops / Training Courses

Third International Training Workshop on “Phage Mu” sponsored by USNSF (1983).

First International Symposium on the applications of Genetic Engineering, sponsored
(1985) by USNSF (INT 8411515/INT 8411516).

A five-week training course on recombinant DNA techniques, organized in
collaboration with NYU Medical Centre (USA) and sponsored (1986) by USNSF
(INT 8521610).

Second International Workshop on the Applications of Genetic Engineering,
sponsored (1986) by USNSF (INT 8600908).

Third International Workshop on the Applications of Genetic Engineering, sponsored
(1987) by USNSF (INT 8607178).

A two-week training course on protein techniques organized in collaboration with
Hatfield Polytechnic (U.K) and sponsored by UGC [UGC(FD)84/86].

Second regional training workshop on recombinant DNA techniques in plant genetic
engineering, organized in collaboration with Johns Hopkins University, Baltimore,
USA and sponsored (1989) by USNSF (INT 870653).

US-Pakistan Workshop on Biotechnology and Conventional Research Needs in
Oilseed crops in collaboration with University of Washington, Seattle, USA and
sponsored (1989) by USNSF (No. INT-8818787).

Second in-country training programme for Agriculture Graduates from the Province
of Balochistan and Azad Kashmir (1987-89) sponsored by USAID, USA.

Fourth in-country training programme for Agricultural personnel from Balochistan and
Northern Areas (1990-91) sponsored by USAID, USA.

A training course on recombinant DNA and advanced microbial technologies held in
Bangladesh and sponsored by UNEP and ISESCO.

A regional training workshop on advanced molecular biology techniques, sponsored
jointly by the Third World Academy of Sciences and UNESCO.

A bi-national symposium on DNA Technology in the improvement of Oilseed crops
sponsored by USNSF (1989).

A US-Pakistan Symposium on Genomics and Computational Biology sponsored by
USNSF (INT 9605034).

Third International Symposium Workshop on the Applications of DNA Technology to
Agriculture and Health, sponsored by Commission of the European Communities.

Fourth International Symposium Workshop on the Applications of Molecular
Biological Research in Agriculture, Health and Environment (1995)

Fifth International Symposium Workshop on the Applications of Molecular Biological
Research in Agriculture, Health and Environment (1997)
11

A training Workshop on Plant Transformation (1998)

First training workshop on Crime Scene and Modern Forensic Techniques in Police
Investigations (January 2003)

NCEMB-COMSTECH International Symposium and Training Workshop on Stem
Cells and Regenerative Medicine (2003)

Second training workshop on Modern Forensic Techniques for crime scene research
and investigation (2004)

A symposium-workshop on Genomics and Computational Analysis (2004)

Third training workshop on Modern Forensic Techniques for Crime Scene Research
and Investigation (2004)

Fourth training workshop on Crime Scene Search and Modern Forensic Techniques
for Crime Scene Research (2005)

Pre - 18th FAOBMB Symposium Satellite Workshop on Bioinformatics (2005)

Fifth, Sixth and Seventh training workshop on Crime Scene Search and Modern
Forensic Techniques for Crime Investigation (Jan, July & Dec 2006 respectively).

BINASIA–Pakistan National Workshop (March 11-12, 2009)

A Symposium workshop on “DNA for Justice” (Dec, 2006).

8th Training workshop on crime scene search and modern forensic techniques for
crime investigation (July 18-19, 2007)

Mini-Symposium on New vistas of plant and medical molecular biology research at
CEMB/CAMB (July 21, 2007)

A mini-symposium on Microscopy and its biological applications (July 24, 2007)

9th Training workshop on crime scene search and modern forensic techniques for
crime investigation (November 21-22, 2007)

National Bioforum 2008 (March,24-28, 2008)

A mini-symposium on CEMB vision 2015 (August 15, 2008)

A mini workshop on Limbal stem cell regeneration therapy (November 05, 2008)

A mini symposium on “Future trends in Molecular Biological Research & its
applications in agriculture and health” (March 25-27, 2009)
MEMBERSHIP OF ADVISORY PANELS AND TECHNICAL COMMITTEES

Member Panel of Experts on Genetic Engineering & Biotechnology constituted by the
United Nations Industrial Development Organization (UNIDO), Vienna, Austria,
(1981-83).

Member Panel of Experts, constituted by the International Agency for Research on
Cancer (IARC), Lyon, France, (1984).

Member, Advisory Panel for "higher education and research in biological sciences",
set up by the Federal Minister of Education (1984-85).

Member, Panel of Experts for "future planning of plant and animal research",
constituted by Advisor to the Prime Minister of Pakistan (1986-88).
12

Member-Secretary National Standing Committee on Biotechnology, Government of
Pakistan (1986-94).

Member, Technical Committee, Pakistan Science Foundation (1987-95).

Member, Technical Committee, National Science Research & Development Board
(1989-93).

Member-Secretary, Expert Group, constituted by the OIC Standing Committee on
Scientific and Technological Cooperation (COMSTECH) on Biotechnology (1990).

Member, Executive Committee of the National Commission for Science and
Technology (1991-94).

Member, National Commission for Science and Technology (1992-95).

Member Board of Trustees of Pakistan Science Foundation (1995-98).

Member, Pakistan Atomic Energy Commission, (1997-2007).

Member, Syndicate, University of Engineering and Technology, Taxila (1999-2003.)

Member, Selection Board, University of Engg. and Technology, Taxila (1999-2003.)

Member, Technical Committee on Biotechnology, Pakistan Science Foundation
(2000-2008).

External Assessor for appointments of Associate Professors/ Professors in the
Faculty of Food Science and Biotech, University of Putra Malaysia (2000-03).

Member, National Biosafety Expert Committee (1998-2003)

Chairman, Sub-Committee of National Biosafety Expert Committee (1998-2003).

Member, National Commission on Biotechnology (2001-08)

Member (GOP Nominee), UNESCO Intergovernmental Bioethics Committee (200108).

Member UNESCO Biodiversity Working Group (2002)

Member, Expert Committee on Pharmaceutical, Federal Ministry of Health,
Islamabad (2003-07)

Member Academic Council, Lahore College for Women University, Lahore (2002-05)

Member, National Vaccine Development and production Task Force (2003- 07)

Member, Council of the Pakistan Academy of Sciences (2003-07)

Member STEDEC Technical Advisory Committee (2003)

Member, Higher Education Commission Awards Committee (2004).

Member Syndicate, University of Health Sciences (2003-06)

Member Board of Studies for the Centre for Biotechnology and Informatics
Balochistan Univ. of information Technology & Management Sciences (2003-06).

Member Technical Advisory Committee, Pakistan Medical Research Council (200306).

Member, Implementation Committee of the University of Health Science to monitor
the progress of HEC funded projects, (2004-07).

Member, Technical Expert Committee of the University of Health Science (2004-07).
13

Member, Board of Governors, Institute of Biotechnology and Genetic Engineering,
NWFP Agriculture University, Peshawar (2004 – 07).

Member, Council of the SESAME (Synchrotron-light for Experimental Science and its
Applications in the Middle East), Cern, Geneva (2004-2008)

Member, Expert Committee on Biochemistry & Molecular Biology, Pakistan Academy
of Science (2005-08).

Member Board of Governors, Centre for Advanced Studies in Vaccinology and
Biotechnology, University of Balochistan, Quetta. (2005-2007)

Member, Board of Trustees, Pakistan Science Foundation (2005-07)

Member, a working group on “Citizen Empowerment and Institutional Reform” for
operationalization of Vision 2030 for Pakistan, Planning Commission, Pakistan
(2008-09)

Member, Committee for making recommendations for suitable teachers under HEC’s
scheme “Best University Teacher Award” for the year 2007 (2008).

Member/ Executive Director, Asia-Pacific International Molecular Biology Network (AIMBN), Seoul, South Korea
 Member National Commission on Science and Technology (2009-2012)
Membership of Editorial Committee/ Board

Physics and Contemporary Needs. Plenum Press, New York (1982-85).

Journal of Molecular and Cellular Biochemistry, Canada (2005-11)
14
RECORD OF RESEARCH WORK:
A.
Scientific Work
Prof. S. Riazuddin started research career in 1964 with M.Sc. thesis work
on "the nutritive value of leaf protein concentrates". These studies showed that a
six hour incubation of defatted leaf protein concentrates at 60 oC, improved the
shelf life of the product without affecting its digestibility when studied by in vitro
protein hydrolysis, using pepsin, trypsin or pancreatic extracts as proteases. In
1967, he started to work for Ph.D. thesis on calcium metabolism. These studies
revealed that elevated mineral requirements in ewes, during such
physiological stress conditions as pregnancy – parturition – lactation, were
met largely by exchangeable calcium pool in the skeleton; and the depleted
mineral skeletal stores of the mother were made good by a gradual
increase in absorption of dietary calcium when physiological stresses
disappeared.
In 1969-70, two Nobel prize discoveries -- that of a site-specific
endonuclease in H.O. Smith's laboratory and a reverse transcriptase by Howard
Temin & David Baltimore aroused his interest in molecular biology. In 1970, he
started post-doctoral research in Professor Bryn Bridges laboratory at the
University of Sussex and continued this work in Pakistan (1971-73). These
studies demonstrated that during bacterial conjugation transfer of donor DNA to
the recipient cells was unaffected by irradiation of the when donor cells by
UV/gamma radiations. He further demonstrated that damaged DNA base
residues transferred from irradiated donor to un-irradiated recipient cells, were
correctly repaired faithfully corrected in repair proficient recipient cells.
Fascinated by the intricacies of cellular repair processes, he joined
Professor L. Grossman's laboratory to investigate the molecular mechanism of
DNA repair in Micrococcus luteus. The choice to employ Micrococcus luteus as a
model bacterial system was based on a) its unusual UV resistance thus offering
a good bacterial source of UV repair enzymes, b) extreme sensitivity to alkylating
chemicals thus providing an ideal cellular system to induce chemical resistance;
and c) low levels of endogenous non-specific endonucleases.
Ultraviolet irradiation of cellular DNA produces mainly cyclobutane type
prymidine-prymidine dimers and a small proportion (1.5%) of 5,6 dihydroxy
dihydrothymine glycol modifications. The lethal and mutagenic effects of UV
radiation can be attributed to inefficient repair of thymine dimers in DNA.
Alkylating chemicals interact with cellular DNA to produce at least 10 different
base modifications having different physiological effects. SN1 type alkylating
chemicals such as dimethyl sulphate (DMS) and methylmethane sulphonate
(MMS) modify ring nitrogens in the purines. SN2 type alkylating chemicals such
as N-methyl-N-nitro-N-nitrosoguanidine (MNNG) exhibit and N-methyl-Nnitrosourea (MNU) high reactivity towards oxygen atoms in DNA. Exocyclic
oxygens in DNA bases and internucleotide phosphate groups are a common site
15
of attack, yielding a variety of alkylation products which have different
physiological effects. Persistence of O6-methylguanine, O4-methylthymine or O2methylthymine has been implicated in the mutagenic/carcinogenic effects of
alkylating chemicals. Most of the studies on alkylation damage and repair had
focussed attention on the physiological fate of ring nitrogen modifications which
constitute about 60-95% of total alkylation products. The fate of highly
carcinogenic exocyclic oxygen modifications had been ignored partly because of
difficulties in their detection and partly due to inducible nature of proteins involved
in the repair of minor alkylation products.
Dr. S. Riazuddin's work on DNA repair in M. luteus made significant
contributions to the understanding of molecular processes leading to eventual
repair of physiologically important base modifications. His works demonstrated
that repair of prymidine-prymidine dimers is initiated by a specific UV
endonuclease- glycosylase enzyme with the introduction of a cleavage at
the N-glycosylic linkage. This enzymatic cleavage results in a base-free
deoxyribose originally associated with the '3 thymine residue of the dimer
and 5' phosphorylated nucleotide. The resulting apurinic site is removed by
cellular apurinic endonucleases. On the other hand, repair of thymine
glycol produced as a minor UV product, is initiated by the introduction of
an endonucleolytic incision on the 5' side of the damaged base residue by
a distinctly separate damage specific endonuclease. The modified base is
removed by the exonucleolytic function of DNA polymerase I. Continuity of
the repaired strand is restored in both cases by the combined functions of
DNA polymerase I and polynucleotide ligase.
Contrary to the mode of UV repair, cellular repair of alkylated DNA
base modifications in M. luteus depends primarily upon the previous
exposure of cells to alkylating chemicals. Pretreatment of cells with
sublethal concentrations of N-methyl-N-nitro-N-nitrosoguanidine induces
5.7 fold increased resistance to the lethal effects of challenge doses of the
same alkylating chemical. This phenomenon termed as “adaptive
response” is accompanied by the synthesis of at least eight proteins. Five
of these proteins are DNA glycosylase enzymes numerically designated as
GI-GV in order of their decreasing molecular weights. GI, is a constitutive
heat labile protein which acts exclusively on 3-methyladenine base
residues in alkylated DNA. GII, which is an inducible protein, acts on 3methyladenine, 3-methylguanine, 7-methyladenine, and 7-methylguanine
with unequal facility. GIII, is another inducible protein which acts on O 2methylthymine and O2-methylcytosine with no activity on methylated
purines (the identity of GIV & GV proteins has yet to be established). The
remaining three proteins, identified as methyl transferases are designated
as TI, TII, TIII enzymes in order of their elution from a Sephadex G75
column. All three methyl transferases have very narrow substrate
specificities with TI acting exclusively on O6-methylguanine base
modifications; T2 acting on O4-methylthymine and T3 showing absolute
specificity for stereo specific phosphotriesters. All of these enzymes have
16
been highly purified, biochemically characterized and their substrates
specificities established. Cellular role of the isolated repair proteins is
being investigated by identifying structural genes of these proteins and
their cloning on multicopy plasmid DNA in E.coli.
Recognizing the absolute need for a reliable and continuous supply of
Type-II restriction enzymes in genetic engineering and recombinant DNA work in
Pakistan, Professor S. Riazuddin initiated a programme to create a local
repository of common molecular tools, and simultaneously searched for new
Type-II specificity restriction enzymes. As part of a collaborative effort, involving
Dr. R.J. Roberts (Nobel Laureate) and his group at Cold Spring Harbor
Laboratory, N.Y., USA, his laboratory has discovered Forty-five new
restriction enzymes present in different microbes. The discovery of these
enzymes have greatly enhanced the powers of genetic manipulations
through recombinant DNA methologies. All the new enzymes have been
entered in International DataBank and two of these enzymes (BseRI and
BscAI) are being marketed by New England Biolabs, Boston, USA.
Dr. S. Riazuddin searched systematically the Pakistani environments
for new and novel isolates of Bacillus thuringiensis, possessing pesticidal
properties against rice stem borers, rice leaf folder, cotton pink ball worm,
chickpea army worm, coleopteral tribolium & rape-seed aphids, and
established a local repository of over 500 isolates varying in genetic makeup and showing different insecticidal properties. Using local isolates as
the source material, cryIA(a), cryIA(b), cryIA(c), cryIII and cryIV genes (old
classification) have been cloned and further manipulated to optimize
expression levels in plants. This provides a precious genetic resource for
genetic engineering of plants for breeding insect resistance.
Dr. S. Riazuddin discovered two novel pesticidal genes which have
larvicidal properties against agronomically important pest such as
nematode, aphids and white fly. Bacillus thuringiensis parasporal proteins
have been previously reported to have insecticidal properties against
lepidopteral and dipteral insects. Dr. Riazuddin has for the first time,
unequivocally demonstrated that pesticidal activity of Bacillus
thuringiensis parasporal protein is not limited to lepidopteral and dipteral
insects, but the activity spectrum extends to other agronomically important
pests. This has opened new avenues of research and is considered a
scientifically important discovery. The excitement may be judged from the
fact that a number of laboratories in Europe, USA and Australia are offering
to collaborate with Dr.Riazuddin in further exploiting the larvicidal potential
of the said novel isolates which are patented in USA, in a joint venture with
Calgene, Inc., Davis, California, USA.
In 1983, Dr. S. Riazuddin attended a 6-week plant molecular biology
course organized by Professor F. Ausubel (Harvard University) at Cold Spring
Harbor Labs, N.Y., USA. His participation in the course introduced him to the
17
revolutionary potential of plant molecular biology. In 1988, he spent a sabbatical
year in Eugene Nester/M.P. Gordon's laboratory in the University of Washington,
Seattle, USA to learn Agrobacterium mediated plant transformation. Recognizing
the obvious role of plant biotechnology in the future economic growth and
development of Pakistan, he initiated a solid and definitive programme in plant
molecular biology to exploit the potential of transgenic plant technologies in
breeding genetic resistance against agronomically important pests in rice, cotton
and chickpea.
To genetically engineer monocotyledonous plants, Dr. Riazuddin
developed a particle acceleration device which is a modification of the
proprietary device marketed by M/s Dupont, USA through BioRad. He used
locally developed device to transform indica rice with Bt pesticidal genes
[cryIA(b), cryIA(c) & CryIIA]. Transgenic indica rice plants exhibit inherent
resistance against rice leaf-folder and rice stem-borer and no undesirable
characteristics, as demonstrated in field trials from 1998 to 2004.
Dr. S. Riazuddin’s laboratory genetically transformed elite local
varieties of cotton (MNH-93 & CIM482) using Agrobacterium-mediated
transformation system with bacterial pesticidal genes from Bacillus
thuringiensis including cryIA(b), cryIA(c) & cryIIA. Genetically transformed
plants show inherent resistance against American bollworm (Heliothis
armigera). In collaboration M/s Ali Akbar Group (Pvt) Ltd, Pakistan, a
private seed company, these crops have been field tested in the cotton belt
areas of Pakistan and are now in national trials for final approval.
Recognizing the revolutionary potential and keidoscopic openings of the
information revealed by the completion of human genome sequences and birth of
associated DNA analytical techniques, Dr. Riazuddin initiated a programme of
studies to elucidate the molecular basis of hereditary genetic disorders, namely
-thalassemia, and hearing & vision impairment in Pakistani population. The
studies on thalassemia established the frequency of specific mutations in
different ethnic groups in Pakistani population. On the basis of this information,
his laboratory developed prenatal diagnostic procedures which are being offered
to local gynaecologists.
The completion of human genome sequence in 2000 was followed by
sequencing of a large number of genomes from microbes to plants and animals,
however, there was little focus on gene functioning. The second half of the
present decade saw a sudden change, while efforts continued on development of
methods for rapid genome sequencing (second generation sequencing),
renewed efforts were directed on determining gene function, using animal
models such as mouse, zebra fish, and fruit fly, and eventually correlating the
malfunctioning with pathological disorders in human beings.
18
During the fist half of the present decade, Dr. Riazuddin’s efforts were
focused predominantly though not exclusively, on homozygosity mapping to
identify new linkages to chromosomal markers that co-segregate with hearing
and vision impairment. The linkage interval was narrowed down and the genes in
the critical interval were rank-ordered on the basis of known/predicted gene
function or expression pattern (in the cochlea or retina). This was a challenging
job when the linkage interval covered a gene rich region and/ or the presence of
genes of nearly ubiquitous pattern of expression such as Actin, Tricellulin, Tight
Junction Proteins. The situation was further complicated when pathogenic alleles
were present in non-coding regions of genes such as those that were found to
occur in Human Growth Factor (HGF). With the advent of parallel sequencing
methods originally conceived by Kraig Ventor, Ham Smith et al., and the
availability of high throughput sequencers, it become possible to quickly and cost
effectively sequence large regions of genes including the non-coding regions.
Recognizing the tremendous powers of new advances in the identification of new
genes, he expanded his studies beyond hearing and visions impairment to
stuttering and metal retardation.
During the second half of the present decade (2005-2010),
Dr. Riazuddin’s lab has made a remarkable progress in the identification of new
genes and studying their functions.
Hearing Impairment: Dr. Riazuddin identified 38 new linkages,
113 pathological mutations and 25 new genes involved in deafness, blindness
and speech disorders. His laboratory characterized the CDH23 and PCDH15
genes, which are essential for both sound and vision transduction. The
identification of truncated mutant alleles of these genes associated with Usher
syndrome type 1 and missense mutations responsible for nonsyndromic hearing
loss, represent very unique genotype-phenotype correlations. Identification of
these genes led to the discovery of founder mutation R245 responsible for the
majority of Usher syndrome type1. This discovery has given new insight into
possible therapy to overcome at least retinal degeneration in patients with Usher
syndrome.
Dr. Riazuddin’s group identified a novel tight junction protein which is
localized at the tricellular contact points of many epithelial cell types. In the inner
ear, tricellulin is concentrated at the tricellular tight junctions of cochlear and
vestibular epithelia, including the structurally complex and extensive junctions
between supporting and neurosensory hair cells.
This work was published in the Am. J. Hum. Genet. 79: 1040-51
(2006); and it received Cotterman recognition and declared as one of
19
two best papers published in the journal during 2006 Further, it was
featured in the Editor’s choice as follows:
Featured in editor’s choice. This paper presents the identification of a
novel tight junction protein that is essential for hearing. Tricellulin is
localized at the tricellular contact points of all epithelial cells and is
presumed to control permeability at this site. However, within the inner
ear as compared to other body tissues, Tricellulin has an elaborate
structure, it localizes within a structurally complex and extensive
junctional network between neurosensory hair cells and the adjacent
supporting cells.
Further studies have developed a knockout mouse model to one of the
corresponding human nonsense mutations. It is anticipated that the proposed
studies will provide a rational molecular framework, useful for engineering novel
therapeutic agents that could effectively target and modulate tight junctions.
Dr. Riazuddin’s group recently paper published in “Cell, vol.141(5), 2010”
that describes, how Actin binding protein TRIOBP forms resilient rootlets of hair
cell stereocilia essential for hearing. The function of rootlets and molecules
responsible for their formation was unknown, however, his studies clearly
showed that TRIOBP, a cytoskeleton associated protein is localized to rootlets
and to uniquely dense bundles reminiscent of rootlets but distinct from bundles
formed by ESPIN, an Actin cross linker in stereocilia. This work is master piece
and the stereocilia pictures in the paper were featured in editor’s choice and on
the cover page of the same issue of “Cell”.
Featured on the Cover page of Cell, in Preview as well as in Faculty
1000 website. This paper significantly extends our knowledge about the
development of hair cell stereocilia rootlets and function of Triobp as a
novel action bundling protein.
Dr. Riazuddin’s work on DFNB73 is the first report (Riazuddin et al., 2009)
on the identification of mutant allele of Barttin causing nonsyndromic hearing loss
in humans and described a unique gentoype-phenotype correlation, which will be
helpful for therapeutic inventions.
This paper was featured on Cover page of American Journal of
Human Genetics 85: 273 (2009) and in Editors' Corner of the of the
same journal.
Vision Impairment: Blindness is a condition which affects all ages and all
societies worldwide. Every year millions lose their eye sight, whereas majority of
these cases are familial and most of them are preventable. Among ocular
dystrophies, cataracts account for majority of the preventable cases of blindness
20
worldwide, followed by retinitis pigmentosa that affecteds 1 in every 3,000
individuals worldwide.
Dr. Riazuddin’s group identified 21 novel loci and genes implicated in the
pathogenicity of congenital cataracts and retinitis pigmentosa. Among these new
causal loci were identified on chromosomes 1, 3, 7, 8, 13 and 19 along with
pathogenic mutations in EPAH2, SIL1, HSF4, GALK1, and
in families
with congenital cataracts. Similarly new loci were identified on chromosome 2,
11, 17 along with causal lesions in RP1, TULP1, PDE6A, PDE6B, ROM1,
MERTK, RLBP1, BBS2, BBS3, BBS8, SLC24A1 and GNAT1 in families with
retinal dystrophies.
Dr. Riazuddin’s group mapped a nuclear family to a region harboring
BBS2, a gene responsible for Bardet-Biedl syndrome. The, sequencing of BBS2
identified a splice acceptor mutation that is expected to result in skipping of the
exon 2, without affecting the amino acid sequence of the protein. His studies
further showed that this exon is present only in the outer nuclear layer of the
retina. This work is published in American Journal of Human Genetics in May
2010.
Dr. Riazuddin’s group mapped a novel locus for autosomal recessive RP
to chromosome 2p, published in American Journal of Ophthalmology. Further
investigation of the critical interval identified the causal mutation in ZNF513,
encoding a presumptive transcription factor. Knockdown of znf513 in zebrafish
reduced the retinal thickness and the expression of rod and cone opsins. Further,
these effects were rescued by co-injection with wild type (wt) but not with the
mutant znf513 mRNA. ChIP analysis showed that only the wild type but not the
mutant Znf513 binds to the Pax6, Sp4, Arr3, Irbp, and photoreceptor opsin
promoters. These results implicated that the ZNF513 C339R mutation is
responsible for RP in this family and also that ZNF513 plays a key role in the
regulation of photoreceptor-specific genes in retinal development and
photoreceptor maintenance. These results appear in the September issue of
American Journal of Human Genetics.
Dr. Riazuddin’s group mapped congenital stationary night blindness
(CSNB) to chromosome 15q and showed a two-base pair deletion in SLC24A1, a
putative sodium calcium channel is the cause of the disease. Expression analysis
using mouse ocular tissues showed that SLC24A1 is expressed in the retina
around postnatal day 7 (P07). Both in situ and immunohistological studies
localized SLC24A1 in the inner segment, outer and inner nuclear layers and
ganglion cells of the retina. In conclusion, these data expand the genetic basis of
CSNB and highlight the indispensible function of SLC24A1 in retinal function
and/or maintenance in humans. These data will appear in the October issue of
21
American Journal of Human Genetics and the paper has been selected for high
lighting in the next AJHG podcast.
--------------------------------Original Message--------------------------From: ees.ajhg.4847.b6266.b2370e09@eesmail.elsevier.com
[mailto:ees.ajhg.4847.b6266.b2370e09@eesmail.elsevier.com] On Behalf Of Robin Williamson
Sent: Monday, August 30, 2010 11:21 AM
To: sriazudd@jhu.edu
Cc: Hejtmancik, James Fielding (NEI)
Subject: AJHG Submission AJHG-D-10-00477R3
Dear Drs. Riazuddin and Hejtmancik,
I am delighted to tell you that your soon to be published AJHG paper entitled "A mutation in SLC24A1
Implicated in Autosomal Recessive Congenital Stationary Night Blindness" has been selected for
highlighting in the next AJHG podcast. I will conduct the interview by telephone. I am hoping that you
will agree to be interviewed and that you might be available during the afternoon on either Tuesday,
September 7th or Wednesday, September 8th. The interview will take about 20 minutes and you will
need to be on a landline, not a cell phone and in a quiet office.
We are only able to interview one person for the podcasts, but we were not sure which one of you to
ask! I hope you do not mind that we are leaving this decision up to you. You are both certainly
welcome to be in the room together during the interview, but due to the sensitivity of the recording
process, we've found that having just one person on the phone is really the only way to get a clear
recording.
Please let me know if one of you would be willing to be interviewed for the AJHG podcast and, if so,
please email me the best time and the appropriate landline phone number on which we can call you.
We look forward to hearing from you!
Sincerely,
Robin
Robin Williamson, Ph.D.,
Deputy Editor, AJHG
Speech Disorders: Stuttering is a disorder in which speech fluency can
be severely compromised. The primary causes of stuttering are not clear,
nevertheless, it is clearly that genetic susceptibility to stuttering is complex, multifactorial and heterogeneous. Dr. Riazuddin’s work (Riazuddin et al., Am. J. Hum.
Gent. 2005) reported a rare example of significant linkage on chromosome 12
from a study on 46 stuttering, consanguineous family. Recently, Dr. Riazuddin’s
work with Dr. Drayna, published in the “New England Journal of Medicine,
362(8):677-685, 2010” described an elegant study on genetic susceptibility to
stuttering in a large consanguineous Pakistani family. After analyzing 45 genes in
several affected persons, they zeroed in on a single nucleotide change (g3598A)
in the GNPT gene. This variant predicts the substitution of lysine at a glutamic
acid residue which is conserved across species and showed the clear evidence
of co-segregation with stuttering in Pakistani family. This is the first example of
clear experimental evidence for the involvement of a metabolic pathway in
speech and that a mutation in the metabolic pathway affects the speech process.
22
This is indeed a unique finding published New England Journal of
Medicine, 362(8):677-685, 2010 and invited editorial by Simon E.
Fisher in the same editor of the journal.
Development of New Methodologies: Dr. Riazuddin’s laboratory has
successfully combined “Target Capture” and “Next Generation Sequencing” in
the accelerated identification of new genes. The paper by Atteeq et al., (America
Journal of Human Genetics 86: 378-88, 2010) describes this powerful
methodology uniquely developed by combining the powers of SNP analysis, high
throughput sequencing and gene capture. He successfully applied this
methodology to identify DFNB 79 gene and demonstrated in unequivocal terms,
how the impracticability of continuing hierarchical approach of sequencing exons
of candidate genes can be successfully overcome in the selection of a candidate
gene. He analyzed a 3Mb region of the DFNB79 interval on chromosome 9 which
included 113 predicted and reported genes. This new and novel approach
identified C9 or F75 designated TPRN as the mutated gene in nonsyndromic
DFNB79. This has given birth to a new, rapid and effective methodology for gene
identification and will greatly accelerate the pace of work in correlating linkages
to new genes and their functions. This work is reported in American Journal of
Human Genetics 86: 378-88, 2010 and the new strategy is being used in a
number of labs including his own lab.
Future Direction of Dr. Riazuddin Research: The above discoveries
reported in high profile research journals during the last five years have raised a
very important question that why does a single base change in the products of
such genes as TRIC (Riazuddin et al., 2006), LRTOMT (Ahmad et al., 2008),
HGF (Schultz, J.M., Khan, S.N., Ahmed, Z.M., Riazuddin, S., et al., 2009), and
variant forms of GNPT and NAGPA enzymes (Kang, C., Riazuddin, S., Mundorff,
J. et al., 2010) which exhibit nearly ubiquitous pattern of expression, only affect
the hearing process and not other processes in the human body? Dr. Riazuddin
has explained the phenomenon that the mutational change affects the
stereoconfiguration of the protein such that it impairs only the specific function
involved in hearing impairment. Dr. Riazuddin’s laboratory proposes to spend
the next five years in finding an answer to this question. Simultaneously, efforts
will continue for a sustained search for new and novel genes by combining the
classical homozygosity mapping and the newly developed “Targeted Capture
and Next Generation Sequencing” methods. The newly isolated genes will be
studied for gene function through development of knockout model,
immunocytochemistry and cellular localization by confocal microscope.
During the last five year, Dr. Riazuddin has also addressed himself to
preconditioning of stem cells. Stem cell transplantation is faced with the
challenge of survival of transplanted cells in the ischemic tissue. The ischemic
23
tissue may not provide the nutrient and oxygen to the transplanted cells. Due to
lack of nutrients, serum deprivation and ischemia, transplanted cells begin to die
within hours after transplantation. Several strategies have been proposed to
enhance the survival of the transplanted cells in the hostile ischemic environment
and one of these strategies is pre-conditioning of stem cells. Various types of
pre-conditioning strategies include: heat shock preconditioning, hypoxic/ischemic
preconditioning, growth factor preconditioning, pharmacological preconditioning,
genetic modulation of stem cells.
Our main finding include preconditioning of BM derived MSCs with injured
liver piece which resulted in enhanced homing and differentiation of
preconditioned MSCs when transplanted in fibrotic liver model. Hence it provides
an improved procedure for treatment of liver fibrosis.
MSCs from diabetic animals are impaired in variety of cellular functions.
IGF1+VEGF preconditioning of diabetes stressed MSCs showed up-regulation of
prosurvival and down-regulation of apoptotic markers. These cells also
demonstrated increased SOD activity, high in-vitro tube forming and chemotactic
ability.
Preconditioning of EPCs with diazoxide for 30minutes improved their
survival against hypoxic injury. EPCs were preconditioned in three groups
comprising of a) control group i.e. non preconditioned EPCs without exposure to
H2O2 hypoxia, EPCs subjected to 200µM H2O2 hypoxic injury and DZ
preconditioned EPCs exposed to 200µM H2O2 hypoxia. DZ preconditioned EPCs
demontrated significantly reduced cell injury, apoptosis and expression of
Caspase-3 and Gsk3β genes. Further, the DZ preconditioning of EPCs upregulated the expression of PI3 kinase, SDF1-α, VEGF, Bcl2 and PCNA genes.
Pharmacological preconditioning of MSCs enhanced their survival and
reduced apoptosis under invitro hypoxia. Further, the transplantation of
preconditioned cells resulted in better recovery of renal function after ischemia in
rats in comparison to non-preconditioned MSCs. In this study, MSCs were preconditioned in three different groups: Pharmacological preconditioned MSCs
(SNAP), MSCs pretreated with pharmacological agent and its blocker (MB) and
Blocker (MB) treated MSCs.
SDF-1α preconditioning of insulin producing cells (IPCs) demonstrated
improved secretion of insulin and proliferation in response to increasing
concentrations of glucose. The preconditioned IPCs also demonstrated
increased cell viability and reduced cell damage.
24
B.
Development Works
In 1981, Dr Riazuddin was selected member of a panel constituted by the
United Nations Industrial Development Organization (UNIDO) to examine the
implications of scientific and technological breakthroughs in genetic engineering
and biotechnology in relation to future economic growth in developing countries.
The panel included Professor R.H. Boyer (California, USA), S. Narang, (Ottawa,
Canada), R. Wu, (Cornell, USA), A. Chakrabarty, (Chicago, USA), A. Bokhari,
(New York, USA), Carl Heden (Stockholm Sweden) and Obaid Siddiqui (India).
In this capacity, he contributed to the preparation of a proposal for the
establishment of an International Centre for Genetic Engineering and
Biotechnology (UNIDO publication No.IS 254). He also prepared a proposal for
the establishment of Centres for Production of DNA Enzymes (UNIDO
publication No.IS 271) and a paper on capability building in biotechnology and
genetic engineering in developing countries (UNIDO publication No.IS 608).
In 1982, he was selected a member of the International Working Group of
Experts in Chemical Carcinogenesis and Related Discipline to evaluate the
carcinogenic risk of some food additives, feed additives, and naturally occurring
substances. As member of the expert working group, he contributed to the
preparation of Vol.31 of the IARC monographs on the Evaluation of Carcinogenic
Risk of Chemicals to Humans.
He has organized training courses and workshops in Bangladesh,
Malaysia and Tunisia to generate a cadre of specifically trained manpower. He
has helped to establish training courses, group meetings, symposia and
seminars in Indonesia, Malaysia, Saudi Arabia and Egypt.
In order to promote the applications of biotechnology in the economic
growth and development of Pakistan, and to build national capability in
biotechnology, he conceived the establishment of an institution for advanced
teaching and research in Molecular Biology that has emerged as one of the best
places of teaching and research in molecular biology in the developing world.
Young scientists from neighbouring developing countries come to spend
sabbatical assignments to receive an exposure to modern approaches in
molecular biology. As a consequence, he and his institution have helped several
developing countries in building a cadre of specifically trained manpower.
Dr. Riazuddin’s group has made outstanding contributions to extend the
applications of his research both in agriculture and health related projects. He
has cloned Bt genes in local elite varieties of cotton to breed insect resistance.
Two cotton varieties with single and double Bt genes are in the national trials for
eventual release to the farmer community. These works will a) help to boost our
cotton production and b) cut down the use of chemical insecticides thus reducing
the environmental burden of hazardous chemicals.
25
Dr. Riazudidn’s work on the cloning of human pharmaceutical protein genes such
as interferon, erythropoietin, interlukin will undoubtedly contribute to improving
health of the general public and simultaneously save huge foreign exchange. His
group has cloned human interferon alfa-2a genes in E. coli, purified the protein at
semi-commercial levels and used the purified products in 3miu injections which
are waiting permission of the Ministry of Health to conduct clinical trials. Hepatitis
is a dreadful disease and number one killer disease in Pakistan. Nearly 10% of
the population is career of hepatitis virus and the import value of interferon has
increased from 1.1 billion in 2005 to 1.4 billion in 2006, 1.87 billion in 2007 and
2.3 billion in 2008 and over 3.00 billion in 2009. The local production will help to
save foreign exchange and simultaneously contribute to health of the general
public thus eventually making Pakistan a “hepatitis free country”.
His work on Bt cotton, interferon and PCR-based diagnostic procedures
has been patented to give this intellectual property due legal protection.
26
PUBLICATIONS BY PROF. S. RIAZUDDIN
National Publications = 57
International Publications = 197
Total Publications = 254
Impact Factor = 896.287
NATIONAL PUBLICATIONS
S.N.
Impact
Factor
Citations
1.
Shah,F.H., Riazuddin, S. and Salam, A. (1967). Effect of heat on the digestibility of
leaf proteins. Pak. J. Sci. Ind. Res. 10: 39-41.
N.A.
N.A.
2.
Riazuddin, S. (1974). Multi-compartmental analysis of calcium kinetics in sheep. I.
Formulation of a four compartmental model. Pak. J. Biochem. 7: 5-9.
N.A.
N.A.
3.
Riazuddin, S. (1974). A model for plasma calcium pools. Pak. J. Biochem., 7: 77-80.
N.A.
N.A.
4.
Riazuddin, S., Shakoori, A.R. and Nawaz, Z. (1986). Mutagenicity testing of some
children's consumables. Pak. J. Zool., 18 (3): 317-326.
N.A.
N.A.
5.
Neelam, M., Nawaz. Z., Shakoori, A.R. and Riazuddin, S. (1987). Isolation and
characterization of R-plasmids from antibiotic resistant bacterial strains collected from
a local hospital. Pak. J. Zool., 19(1): 17-26.
N.A.
N.A.
6.
Shakoori, A.R., Akhtar, R., Nawaz, Z. and Riazuddin, S. (1987). Isolation and
biochemical characterization of Rhizobium species collected from Lahore. Pak. J.
Zool., 19(2): 185-191.
N.A.
N.A.
7.
Neelam, M., Nawaz, Z. and Riazuddin, S.(1987). Hydrocarbon Biodegradation:
Biochemical characterization of bacteria isolated from local soils. Pak. J. Sci. & Ind.
Res., 30(5): 382-385.
N.A.
N.A.
8.
Riazuddin, S. and Ahmed, Z. (1987). A non-pyrimidine dimer UV-damage specific
endonuclease. I. Isolation and purification. Pak. J. Zool. 19 (2): 193-209.
N.A.
N.A.
9.
Riazuddin, S., Athar, A. and Shakoori, A.R. (1987). A non-pyrimidine dimer UVdamage specific endonuclease. II. Mechanism of action. Pak. J. Zool. 19 (3): 217230.
N.A.
N.A.
10.
Riazuddin, S., Sohail, A., Maqbool, T., Khan, E. and Mushtaq, R. (1987). Presence of
new Type-II specificity restriction enzymes in local bacteria. Pak. J. Sci. & Ind. Res.
30 (11): 819-824.
N.A.
N.A.
11.
Athar, A., Riazuddin, S. and Lali, S.M. (1987). Self methylation of correctional
transferases during repair of O6 methyl guanine, O4 methyl thymine and methyl
phosphotriesters. Pak. J. Zool. 19 (4): 413-424.
N.A.
N.A.
12.
Riazuddin, S., Sohail, A., Nawaz, Z., Mushtaq, R. and Shakoori, A. R. (1987).
Isolation of a temperate bacteriophage OR7 specific for Pseudomonas aeruginosa.
Pak. J. Zool. 19 (4): 425-435.
N.A.
N.A.
13.
Sohail, A., Amjad, M., Shakoori, A.R. and Riazuddin, S. (1987). An improved method
for isolation of covalently closed circular DNA from ox174am3 phage. Pak. J. Zool.
19(4): 407-412.
N.A.
N.A.
14.
Sohail, A., Mushtaq, R., Khan, E., Maqbool, T. and Riazuddin, S. (1987). Discovery of
two new Type-II restriction enzymes. Pak. J. Zool. 19(4): 371-391.
N.A.
N.A.
27
15.
Riazuddin, S., Athar, A., Sohail, A. and Ahmad, Z. (1987). Molecular mechanism of
host controlled Restriction and Modification in Haemophilus influenzae. I. Isolation of
restriction positive mutant. Pak. J. Zool. 19(4): 393-405.
N.A.
N.A.
16.
Robin, S.P. Ghauri, M.A., Jabeen, F., Riazuddin, S. and Shams, F.A. (1987). Isolation
and characterization of acidophilic bacteria from the local environment. Pb. Univ. J.
Zool., 2: 1-9.
N.A.
N.A.
17.
Riazuddin, S., Husnain, T., Malik, T., Farooqi, H. and Abbas, T. (1988). Establishment
of Callus-Tissue Cultures and the induction of organogenesis in Chick pea (Cicer
arietinum L.). Pak. J. Agri. Res., 9: 339-345.
N.A.
N.A.
18.
Riazuddin, S. and Ahmad, Z. (1988). Formation of homopyrimidine dimer during UV
irradiation of drug bound DNA. Pak. J. Phar. 1 (1 & 2): 1-8.
N.A.
N.A.
19.
Athar, A. Shakoori, A.R. and Riazuddin, S. (1988). Partial purification of O2-MeTGlycosylase enzyme induced during chemical adaptation of M. luteus. Pak. J. Zool.,
2(20): 159-172.
N.A.
N.A.
20.
Butt, A.I., Riazuddin, S., Shakoori, A.R. and Jabeen, F. (1988). Isolation and
identification of petroleum hydrocarbon degrading bacteria from the local environment.
Pak. J. Zool., 20(4): 391-399.
N.A.
N.A.
21.
Farooqi, H., Islam, I., Haider, H. and Riazuddin, S. (1990). Tissue culture of chickpea
and its transformation by Agrobacterium tumefaciens. Prof. Intl. Telecommunication
Symposium on Plant Biotechnology, Islamabad, Pakistan, p.39.
N.A.
N.A.
22.
Khan, E. Mateen, A., Rubin, S., Karim, S., Makhdoom, R., Sohail, A. and Riazuddin,
S. (1994). Entomocidal activity of local isolates of B. thuringiensis. Pak. J. Agri. Res.
15: 297-301.
N.A.
N.A.
23.
Humera, F., Malik T., Islam, R. and Riazuddin, S. (1994). Tissue culture of chickpea
and its transformation by Agrobacterium tumefaciens. Pak.J. Agri. Res. 15: 317-319.
N.A.
N.A.
24.
Khan, S.N., Zafar, A.U. and Riazuddin, S. (1995). Molecular Genetic diagnosis of thalassemia in Pakistan. J. Pak. Med. Asso. 45(3): 66-70.
N.A.
N.A.
25.
Husnain, T., Khanum, F. ,Riazuddin, S. and Gordon, M.P. (1995). Transformation of
Basmati rice (Oryza sativa Linn) with bacterial genes by particle bombardment. Pak. J.
Plant Sci. 1(2): 219-228.
N.A.
N.A.
26.
Khan, E., Makhdoom R. Karim, S. and Riazuddin, S. (1993). Entomocidal activity of
indigenous Bt isolates against two important rice pests, Tryporyza incertulus and
Cnaphalocrocis medinalis. Proceedings of International Symposium on Biotechnology
for sustainable development, Dec. 15-20, 1993, NIBGE, Faisalabad, Pakistan.
N.A.
N.A.
27.
Khan, E., Karim, S., Makhdoom, R., and Riazuddin, S. (1995). Abundance,
distribution and diversity of Bacillus thuringiensis in Pakistanian environment. Pak. J.
Sci. Ind. Res. 38(5-6): 192-195.
N.A.
N.A.
28.
Khan S.N., Zafar, A.U. and Riazuddin, S. (1995). Molecular genetic diagnosis of thalassemia in Pakistan. J. Pak. Med. Assoc. 45(3): 66-70.
N.A.
N.A.
29.
Husnain, T. Riazuddin, S. (1996). Transformation of Gossypium hirsutum variety
MNH93 with insect resistant gene. Proceedings of First Workshop on the Management
of Cotton Leaf Curl Virus "March 11-12, 1996.
N.A.
N.A.
30.
Karim, S. and Riazuddin S. (1997). Bacillus thuringiensis delta-endotoxins: Molecular
mechanism of action and pest management. Pak. Acad. Sci. 34(2): 135-155.
N.A.
N.A.
31.
Makhdoom, R., Karim, S. and Riazuddin S. (1997). Pathogenicity of locally isolated
Bacillus thuringiensis delta-endotoxins against Earias vitella and Helicoverpa armigera.
N.A.
N.A.
28
Pak. Entomol. 19 (1-2): 1-7
32.
Karim, S., Makhdoom, R. and Riazuddin S. (1997). Specificity and pesticidal activity
of Pakistani isolates of Bacillus thuringiensis towards Pectinophora gossypiella. Pak.
Entomol. 19(1-2): 22-26.
N.A.
N.A.
33.
Khanum, F., Husnain, T., Riazuddin, S. and Gordon, M.P. (1997).
regeneration of Basmati rice. Pak. J. Biochem. 30: 22-26.
In vitro
N.A.
N.A.
34.
Karim, S. and Riazuddin S. (1998). A new soybean diet for the rearing of cotton pink
bollworm (Pectinophora gossypiella). Pak. Entomol. 19(1-2): 31-38.
N.A.
N.A.
35.
Haris, W.A.A., Husnain, T., Riazuddin, S. (1998). Transformation of cotton
(Gossypium hirsutum L) insect resistance gene by particle bombardment and
agrobacterium. Pak. J. Biol. Sciences 1(3): 170-174.
N.A.
N.A.
36.
Husnain, T., Khanum, F., Fatima, T., Khan, E., Riazuddin, S. and Altosaar, I. (1998).
Transforamtion of indica rice with synthetic Cry1A(c) Gene. Biologia 44(1&2): 180192.
N.A.
N.A.
37.
Idrees, M., Bhattti, A.H., Khan, S.U. and Riazuddin, S. (1998). Drug Resistance in
Mycobacterium tuberculosis isolated from a group of patients referred to PMRC
Research Centre at Mayo Hospital, Lahore, Pakistan. Pak. J. Zool. 30: 335-339.
N.A.
N.A.
38.
Malik, K., Butt, A. and Riazuddin, S. (1998). Toxicity of Bacillus thuringiensis strains to
the cotton aphid, Aphis gossypii (Homoptera : Aphidiae). Punjab Univ. J. Zool., 13:
191-194.
N.A.
N.A.
39.
Shahid A.A., Latif, Z. and Riazuddin, S. (1998). Comparison of phytotoxin(s)
production among two isolates of Ascochyta rabiei varying in virulence. Pak. J. Pl.
Sci., 4(1): 1-11.
N.A.
N.A.
40.
Chaudhry, B. and Riazuddin, S. (1998). Cloning and expression Micrococcus luteus
glycosylase repair genes in E. coli. Pak J. Biochem. and Mol. Bio: 31: 29-43.
N.A.
N.A.
41.
Karim, S. and Riazuddin, S. (1999). Rice insect pests of Pakistan and their control: A
lesson from past from sustainable future integrated pest management. Pak. J. Biol.
Sci. 2(2): 261-276.
N.A.
N.A.
42.
Karim, S. Murtaza, M. and Riazuddin, S. (1999). Field evaluation of Bacillus
thuringiensis, insect growth regulators, chemical pesticide against Helicoverpa
armigera (Huber) LepidopteraL Noctuidae) and their compatibility for integrated pest
management. Pak. J. Biol. Sci. 2(2): 320-326.
N.A.
N.A.
43.
Rizwana A., Karim, S. and Riazuddin S. (1999). Entomocidal activity of Bacillus
thuringiensis transgenic rice plants against rice leaf-folder, Cnaphalocrocis medinalis
(Lepidoptera: Pyralidae). Pak. J. Biol. Sci. 2(4): 1472-1477.
N.A.
N.A.
44.
Zafar, A.U., Karim, S., Nasir, I.A. and Riazuddin, S. (2000). Shelf life and field
evaluation of CAMB Bacillus thuringiensis Biopesticide against Helicoverpa armigera
(Hubner) (Lepidoptera: noctuidae) on Tomato. Pak. J. Biol. Sci. 3(5): 804-807.
N.A.
N.A.
45.
Karim, S., Zafar, A.U., Nasir, I.A. and Riazuddin, S. (2000). Field Efficcacy of CAMB
Bacillus thuringiensis to Control Helicoverpa armigera (Hubner) and Earias vitella
(Fabricius) in Okra Crop. Pak. J. Biol. Sci. 3(8): 1296-1298.
N.A.
N.A.
46.
Noor, S. Husnain, T. and Riazuddin, S. (2000) Screening of putative transgenic rice
and cotton plants: a simple and easy method. Pak. J. Biological Sciences 3: 591593.
N.A.
N.A.
47.
Malik, K., Mahmood, T. and Riazuddin, S. (2001). The receptor for Bacillus
thuringiensis Cry 1Ac delta-endotoxin in the Brush Border Membrane of the
N.A.
N.A.
29
Lepidopteran Hlicoverpa armigea in Aminopeptidase N. J. Biol. Sci. 1(8): 782-84.
48.
Khan. M.A. Makhdoom, R., Husnain, T. Malik, K. Latif, Z. Altosaar, I. and Riazuddin,
S. (2001) Expression of Bt gene in a dicotyledonous plant under a promoter derived
from monocotyledonous plant. Pak. J. Biol. Sciences 4(12): 1518-1522.
N.A.
N.A.
49.
Husnain, T., Bokhari, S.M. Riaz, N., Fatima, T., Shahid, A.A., Bashir K., Jan, A. and
Riazuddin, S. (2003). Pesticidal genes of Bacillus thuringiensis in transgenic rice
technology to breed insect resistance. Pak. J. Biochem. Mol. Biol. 36(2): 133-142
N.A.
N.A.
50.
Mahmood, N., Ali, R.M., Husnain, T., Hussain, S.S., Majeed, A. and Riazuddin, S.
(2003). Biolostic transformation of Gossypium hirsutum L variety CIM-482 with cry1Ac
gene gene under wound inducible promoter Pakistan. Pak. J. Biochem. Mol. Biol.
36(2): 146-157.
N.A.
N.A.
51.
Ali, R.M., Husnain, T., Hussain, S.S., Mahmood N. and Riazuddin. S. (2004). Multiple
Shoot Regeneration Response of Recalcitrant Cotton (Gossypism hirsutum L.) Cultivar
CIM-443. Pak. J. Biol. Sci. 7(8): 1371-75.
N.A.
N.A.
52.
Bashir, K., Husnain, T. and Riazuddin, S. (2004) Response of transgenic rice
expressing two Bt genes to nontarget insects. IRRN 29(2):15-16.
N.A.
N.A.
53.
Rasheed, S., Fatima, T., Husnain, T., Bashir, K., Riazuddin, S. (2005). RAPD
characterization of somaclonal variation in indica basmati rice. Pak. J. Bot., 37(2):
249-262
0.106
N.A.
54.
Malik, K., Riazuddin, S.A., and Riazuddin, S. (2006). Identification, purification,
cloning and expression of a novel receptor for Bacillus thuringiensis Cry1A deltaendotoxins in the brush border membranes of the Helicoverpa armigera (Lepidoptera:
Noctuidae). Pak. J. Bot., 38(3): 767-778.
0.106
N.A.
55.
Malik, K. and Riazuddin, S. (2006). Immunoassay-based approach for detection of
novel Bacillus thuringiensis delta-endotoxins, entomocidal to cotton aphids (Aphis
gossypii) and whiteflies (Bemisia tabaci). Pak. J. Bot., 38(3): 757-765
0.106
N.A.
56.
Hussain, S.S., Husnain, T. and Riazuddin, S. (2007). Sonication assisted
Agrobacterium mediated transformation (SAAT): An alternative method for cotton
transformation. Pak. J. Bot., 39(1): 223-230.
0.106
N.A.
57.
Latif, Z., Idrees, A.N. and Riazuddin, S. (2007). Indigenous production of synthetic
seeds in Daucus carota. Pak. J. Bot., 39(3): 849-855.
0.106
N.A.
Total:
0.530
30
INTERNATIONAL PUBLICATIONS
S.N.
Impact
Factor
Citations
1.
Braithwaite, G.D., Glascock, R.F. and Riazuddin, S. (1969). Calcium metabolism in
lactating ewes. Br. J. Nutr., 23: 827-834.
2.708
65
2.
Braithwaite, G.D., Glascock, R.F. and Riazuddin, S. (1970). Calcium metabolism in
pregnant ewes. Br. J. Nutr., 24: 661-670.
2.708
71
3.
Braithwaite, G.D. and Riazuddin, S. (1971). The effect of age and level of dietary calcium
intake on calcium metabolism in sheep. Br. J. Nutr., 26: 215-225.
2.708
69
4.
Green, M.H.L., Bridges, B.A. and Riazuddin, S. (1971). Effect of gamma radiation on the
donor ability of recA and recA+strains of Escherchia coli. J. Gen. Microbiol., 67: 63-68.
0.766
4
5.
Braithwaite, G.D., Glascock, R.F. and Riazuddin, S. (1972). Studies on the transfer of
calcium across the ovine placenta and incorporation into the foetal skeleton. B. J. Nutr., 27:
417-424.
2.708
26
6.
Braithwaite, G.D., Glascock, R.F. and Riazuddin, S. (1972). Effect of hexoestrol on calcium
metabolism in the sheep. Br. J. Nutr., 28: 269-273.
2.708
15
7.
Riazuddin, S. and Muhammad, A. (1974). Transfer of pyrimidine dimers during bacterial
conjugation. Photochem. Photobiol., 19: 169-172.
2.061
0
8.
Riazuddin, S. and Grossman, L. (1977). Micrococcus luteus correndonucleases. I.
Resolution and purification of two endonucleases specific for DNA containing pyrimidine
dimers. J. Biol. Chem., 252 (18): 6280-6286.
5.808
84
9.
Riazuddin, S. and Grossman, L. (1977). Micrococcus luteus correndonucleases. II.
Mechanism of action of two endonucleases specific for DNA containing pyrimidine dimers.
J. Biol Chem., 252 (18): 6287-6293.
5.808
26
10.
Riazuddin, S. and Grossman, L. (1977). Micrococcus luteus correndonucleases. III
Evidence for involvement in repair in vitro of two endonucleases specific for DNA containing
pyrimidine dimers. J. Biol. Chem., 252 (18): 6294-6298.
5.808
16
11.
Riazuddin, S. and Lindahl, T. (1978). Isolation of a DNA glycosylate enzyme. Mutation
Res., 47: 167-168.
4.111
12.
Riazuddin, S. and Lindahl, T. (1978). Properties of 3-methyladenine DNA glycosylase from
Escherchia coli. Biochem., 17: 2110-2118.
3.633
13.
Riazuddin, S. and Grossman, L. (1978). Purification of a damage specific endonuclease.
Mutation Res., 47: 165-166.
4.111
14.
Grossman, L., Riazuddin, S., Haseltine, W. A. and Lindan, C. (1978). Nucleotide excision
repair of damaged DNA . CHS Symp. Quant. Biol., 43: 947-955.
0.896
15.
Riazuddin, S. (1980). Purification and properties of pyrimidine dimer
endonucleases from Micrococcus luteus. Methods Enzymol., 65: 185-191.
specific
1.640
16.
Riazuddin, S. (1980). Purification and properties of an endonuclease specific for nonpyrimidine dimer damage induced by ultraviolet radiations. Methods Enzymol. 65: 231235.
1.640
31
166
25
17.
Riazuddin, S. (1980). Purification and properties of a 3-methyladenine DNA glycosylase
from Escherchia coli. Methods Enzymol., 65: 290-295.
1.640
18.
Riazuddin, S., Athar, A. and Ahmed, Z. (1982). Repair of alkylation damages in M. luteus.
"Proceeding of Int'l. Meeting on Inducible Repair, Tolouse, France."
N.A
19.
Athar, A., Ahmad, Z. and Riazuddin, S. (1984). Adptive response of M. luteus to alkylating
chemicals. Nucleic Acids Res., 12(4): 2111-2126.
6.317
27
20.
Riazuddin, S., Athar, A. and Saffhil, R. (1985). Chemical adaptation of M.luteus induces
repair functions for O-alkylated DNA pyrimidines. Nucleic Acids Res., 13 (19): 7153-7166.
6.317
4
21.
Riazuddin, S., Malik, M.M. and Nasim, A. (1987). Mutagenicity testing of some medicinal
herbs. Environ Mol Mutagen., 10: 141-148.
2.653
4
22.
Riazuddin, S., Athar, A.,Ahmed, Z., Lali, S.M. and Sohail, A. (1987). DNA glycosylase
enzymes induced during chemical adaptation of M. luteus. Nucleic Acids Res., 15 (16):
6607-6624.
6.317
4
23.
Riazuddin, S., Athar, A. and Sohail, A. (1987). Methyltransferases induced during chemical
adaption of M. luteus. Nucleic Acid Res., 15 (22): 9471-9486.
6.317
6
24.
Riazuddin, S. (1988). Development of training programmes in biotechnology safety and
risk assessment. BioEssays, 9(4): 131-132.
5.965
0
25.
Riazuddin, S. (1988). Repair of Alkylated DNA in M. luteus. Proceedings of the Second
Italy-Japan Joint Seminar on Biological Sciences. pp.70-71.
N.A
26.
Riazuddin, S., and Nasim, A., (1989). Future growth in biotechnology in the developing
countries. Genome, 31(2):1042-5.
1.972
27.
Islam, R., and Riazuddin, S. (1992). Induction of tumor formation in chickpea tissue by
treatment with Agrobacterium tumefaciens. Bangladesh J. Microbiology, 9: 31-33
N.A
28.
Islam, R., and Riazuddin, S. (1992). In vitro induction of roots in chickpea by
Agrobacterium rhizogenes. Plant Tissue Culture (Bangladesh), 2: 135-137.
0.631
29.
Islam, R. and Riazuddin, S. (1992). Transformation of chickpea tissue by A. tumefaciens
in ex-vitro conditions. International Chickpea Newsletter, 26: 11-12.
N.A
30.
Latif, Z., Strange, R.N., Bilton, J. and Riazuddin, S. (1993). Production of the phytotoxins,
solanapyrones A and C and cytochalasin D among none isolates of Ascochyta rabiei.
Plant Pathol., 42: 172-180.
2.198
8
31.
Mushtaq, R., Naeem, S., Sohail, A. and Riazuddin, S. (1993). BseRI a novel restriction
endonuclease from a Bacillus species which recognizes the sequence 5'....GAGGA....3'.
Nucl. Acids Res., 21: 3585.
6.317
0
32.
Islam, R. and Riazuddin, S. (1993). Virulence of Agrobacterium tumefaciens on chickpea
(Cicer arietinum L.). Bangladesh J. Botany, 22(2): 233-236.
0.048
0
33.
Islam, R., Farooqi, H. and Riazuddin, S. (1993). In vitro organogenesis of chickpea and its
transformation by Agrobacterium tumefaciens. Plant Tissue Culture (Bangladesh), 3(1):
29-34.
0.631
34.
Islam, R. and Riazuddin, S. (1993). Effect of genotype and age of seedling on compatible
reaction between chickpea and Agrobacterium rhizogenes. Bangladesh J. Microbiology,
10: 29-32.
N.A
35.
Islam, R. and Riazuddin, S. (1993). Shoot organogenesis in chickpea (Cicer arietinum L.).
J. Bioscience, 1: 1-5.
0.966
32
0
36.
Islam, R. and Riazuddin, S. (1993). In vitro rooting on micropropagated shoots of
chickpea. International Chickpea Newsletter, 29: 21-23.
N.A
37.
Islam, R. and Riazuddin, S. (1993). Isolation of genomic DNA from in vitro grown chickpea
(Cicer arietinum L.) tissues. International Chickpea Newsletter, 29: 23-25.
N.A
38.
Islam, R., Malik, T., Husnain, T. and Riazuddin, S. (1994). Strain and cultivar specificity in
the Agrobacterium-chickpea interaction. Plant Cell Reports, 13: 561-563.
1.727
39.
Islam, R. and Riazuddin, S. (1994). Influence of genotype and age of seedlings on
chickpea response to Agrobacterium tumefaciens. International Chickpea Newsletter, 1:
23-25.
N.A
40.
Islam,R. and Riazuddin, S. (1994). Chages in protein, RNA and DNA content of in vitro
propagated calli of chickpea (Cicer arietinum L). Bangladesh J. Bot. 24: 87-89.
N.A
41.
Riazuddin, S., Husnain, T., Khan, E. and Khanum, F. (1995). Insect resistant transgenic
Basmati rice. Rice Biotechnology Quarterly, 23: 7-8.
N.A
42.
Islam, R., Farooqui, H. and Riazuddin, S. (1995). Clonal propagation from seedling nodes
and shoot apices of chickpea (Cicer arietinum L.). Plant Tissue Culture (Bangladesh) 5(1):
53-58.
N.A
43.
Husnain, T., Malik, T., Riazuddin, S. and Gordon. M.P. (1997). Studies on the expression
of marker genes in chickpea. Plant Cell Tiss. Org., 49: 7-16.
0.951
8
44.
Khan, S.N. and Riazuddin, S. (1998). Molecular Characterization of -thalassemia in
Pakistan. Hemoglobin, 22(4): 333-345.
0.843
6
45.
Khanum, F., Husnain, T. and Riazuddin, S. (1998). Effect of age of seedling and
phytohormones on micropropagation of indica rice (oryza sativa L.) from meristem culture.
J. Plant Biol,. 41(2): 93-96.
0.670
46.
Karim, S., Nasreen, Z., Malik, K. and Riazuddin, S. (1998). A simple and fast method for
mass rearing of sweet potato whitefly (Bemisia tabaci) and cotton aphid (Aphis gossypii) in
contained conditions. Asia Life Science, 7(1): 103-108.
N.A
47.
Maqbool, S., Husnain, T., Riazuddin, S., Masson, L. and Christou, P. (1998). Effective
control of yellow stem borer and rice leaf-folder in transgenic rice indica varieties Basmati
370 and M7 using the novel delta-endotoxin cry2A Bacillus thuringiensis gene. Mol. Breed.,
4(6): 501-507.
2.357
48.
Islam, R., Farooqui, H. and Riazuddin, S. (1998). In vitor genotype phytohormone interaction
in chickpea. Plant Tissue Culture (Bangladesh) 8(2): 173-175.
N.A
49.
Islam, R., Farooqui, H. and Riazuddin, S. (1999). Improved efficiency in chickpea tissue
culture: Effects of
presoaking and age of explants on in vitro shoot proliferation. Intl.
chickpea & Pigeonpea Newslet. (India) 6: 36-37.
N.A
50.
Karim, S., Malik, K., Zafar, U. and Riazuddin, S. (1999). Evaluation of Pakistanian Bacillus
thuringiensis isolates Scirpophaga incertulas and Cnaphalocrocis medinalis. J. Asia
Pacific Entomol., 2(1): 61-67.
N.A
51.
Karim, S., Malik, K., Zafar, U. and Riazuddin, S. (1999). Efficacy of activated toxins from
Pakistanian Bacillus thuringiensis isolates to rice yellow stem borer (Scirpophaga
incertulas) and leaf folder (Cnaphalocrocis medinalis). J. Asia Pacific Entomol., 2(1): 1-7.
N.A
52.
Karim, S., Riazuddin, S., and Dean, D.H. (1999). Interaction of Bacillus thuringiensis deltaendotoxins with midgut brush border membrane vesicles of Helicoverpa armigera. J. Asiapacific Entomol., 2(2): 153-162.
N.A
33
5
28
53.
Chaudhry, B. Yasmeen, A., Husnain, T. and Riazuddin, S. (1999). Mini-scale genomic
DNA extraction from cotton. Plant Molecular Biology Reporter (17): 1-7.
0.625
0
54.
Karim, S., Riazuddin, S., Gould, F. and Dean, D.H. (2000). Determination of receptor
binding properties of Bacillus thuringiensis delta-endotoxins to cotton bollworm (Helicoverpa
zea) and pink bollworm (Pectinophora gossypiella) midgut brush border membrane
vesicles. Pesticide Biochem. Physiol., 67(3): 198-216
1.189
11
55.
Friedman, T.B., Battey, J., Kachar, B., Riazuddin, S., Noben-Trauth, K., Griffith, A. and
Wilcox, E.R. (2000). Modifier genes of hereditary hearing loss. Current Opinion in
Neurobiology, 10: 487-493.
9.286
20
56.
Majeed, A., Husnain, T. and Riazuddin, S. (2000). Transformation of Virus Resistant
Genotype of Gossypium hirsutum L., with Pesticidal Gene. Plant Biotech., 17(2): 105-110.
N.A
57.
Riazuddin, S., Castlelein, C.M., Ahmad, Z.M., Lalwani, A.K., Mastroianni, M.A., Naz, S.,
Smith, T.N., Liburd, N.A., Friedman, T.B., Griffith, A.J., Riazuddin, S., Wilcox, E.R. (2000).
Dominant modifier DFNM1 suppresses recessive deafness DFNB26. Nat. Genet., 26: 431434.
25.556
48
58.
Khan, S.N., Riazuddin, S. and Galanello, R. (2000). Identification of three rare Thalassemia mutations in the Pakistani Population. Hemoglobin, 24(1): 15-22.
0.516
7
59.
Khan, S.N., Riazuddin, S., Butt, F.I. and Galanello, R. (2000). Hb Sallanches [alpha
104(G11)Cys -> Tyr]: a rare alpha 2-globin chain variant found in the homozygous state in
three members of a Pakistani family. Hemoglobin, 24(1): 31-35.
0.843
4
60.
Old, J., Khan, SN., Verma, I., Fucharoen, S., Kleanthous, M., Iaonnou, P., Kotea, N.,
Fisher, C., Riazuddin, S., Saxena, R., Winichagoon, P., Kyriacov, K., Quobaili, FA., and
Khan, B. (2001). A multi-centre study in order to define further the molecular basis of thalassemia in Thailand, Pakistan, Sri Lanka, Mauritius, Syria and India and to develop a
simple molecular diagnostic strategy by ARMS-PCR. Hemoglobin, 25(4): 397-407.
0.843
12
61.
Bork, J.M., Peters, L.M., Riazuddin, S., Bernstein, S.L., Ahmed, Z.M., Ness, S.L.,
Polomeno, R., Ramesh, A., Schloss, M., Srisailpathy, C.R., Wayne, S., Bellman, S.,
Desmukh, D., Ahmed, Z.M., Khan, S.N., Kaloustian, V.M., Li, X.C., Lalwani, A., Riazuddin,
S., Bitner-Glindzicz, M., Nance, W.E., Liu, X.Z., Wistow, G., Smith, R.J., Griffith, A.J.,
Wilcox, E.R., Friedman, T.B. and Morell, R.J. (2001). Usher syndrome 1D and
nonsyndromic autosomal recessive deafness DFNB12 are caused by allelic mutations of
the novel cadherin-like CDH23. Am. J. Hum. Genet., 68: 26-37.
11.092
154
62.
Wilcox, E.R., Burton, Q.L., Naz, S., Riazuddin, S., Smith, T.N., Ploplis, B., Belyantseva, I.,
Ben-Yosef, T., Liburd, N.A., Morell, R.J., Kachar, B., Wu, D.K., Griffith, A.J., Riazuddin, S.,
and Friedman, T.B. (2001). Mutations in the Gene Encoding Tight Junction cladin-14
Cause Autosomal Recessive Deafness DFNB29. Cell, 104: 165-172.
29.194
139
63.
Maqbool, S.B., Riazuddin, S., Loc, N.T., Gatehouse, A.M.R., Gatehouse, J.A. and
Christou, P. (2001). Expression of multiple insecticidal genes confers broad resistance
against a range of different rice pests. Mol. Breed., 7: 85-93.
2.357
46
64.
Ben-Yousef, T, Wattenhofer, M., Riazuddin, S., Ahmed, Z.M., Scott, H.S., Kudoh, J.,
Shibuya, K., Antonarakis, S.E., Bonne-Tamir, B., Radhakrishna, U., Naz, S., Ahmad, Z.,
Riazuddin, S., Pandya, A., Nance, W.E., Wilcox, R.R., Friedman, T.B. and Morell, R.J.
(2001). Novel mutations of TMPRSS3 in four DFNB8/B10 families segregating congenital
autosomal recessive deafness. J. Med. Genet., 38(6): 396-403.
5.535
18
65.
Ahmed, Z.M., Riazuddin, S., Bernstein, S.L., Ahmad, Z., Khan, S.N., Griffith, A.J., Morell,
R.J., Friedman, T.B., Riazuddin, S. and Wilcox, E.R. (2001). Mutations of Protocadherin
Gene PCDH15 Cause Usher Syndrome Type 1F. Am. J. Hum. Genet., 69(1): 25-34.
11.092
108
34
66.
Liburd. N., Ghosh. M., Riazuddin, S., Naz, S. Khan, SN., Ahmed, Z., Riazuddin, S., Liang,
Y., Menon, PSN., Smith, T., Smith, ACM., Chen, KS., Lupski, JR., Wilcox, ER., Potocki, L
and Friedman, TB. (2001). Novel mutations MY015A associated with profound deafness in
consanguineous families and moderately-severe hearing loss in a smith-Magenis syndrome
subject. Hum. Genet., 109: 535-541.
3.974
67.
Friedman, T.B., Hinnat, J.T., Ghosh, M., Boger, E.T., Riazuddin, S., Lupski, J.R., Potocki,
L., Wilcox, E.R. (2002). DFNB3, Spectrum of MYO15A recessive mutant allele and an
emerging genotype-phenotype correlation. Adv. Otolaryyngol., 61: 124-130.
0.346
68.
Bork, J.M., Morell, R.J., Khan, S., Riazuddin, S., Wilcox, E.R., Friedman, T.B. and Griffith,
A.J. (2002). Clinical presentation of DFNB12 and Usher Syndrome Type 1D. Adv.
Otorhinolaryngol., 61: 145-152.
0.346
69.
Ahmed, Z.M., Riazuddin, S., Friedman, T.B., Riazuddin, S., Wilcox, E.R. and Griffith, A.J.
(2002). Clinical Manifestations of DFNB29 Deafness. Adv. Otolaryyngol., 61: 156-160.
0.346
70.
Riazuddin, S., Ahmed, Z.M, Friedman, T.B., Griffith, A.J. Riazuddin, S. and Wilcox, E.R.
(2002). Genetic Modifiers of Hereditary Hearing Loss. Adv. Otolaryyngol., 61: 224–229.
0.346
71.
Husnain T., Asad, J., Maqbool, S.B., Datta, S.K. and Riazuddin, S. (2002). Variability in
expression of insecticidal Cry1Ab gene in Indica Basmati rice. Euphytica, 128: 121-128.
0.907
11
72.
Kurima K, Yang Y, Riazuddin S, Ahmed Z, Naz S, Mo J, Makishima T, Ghosh M, Menon
SN, Deshmukh D, Oddoux C, Ostrer H, Khan S, Riazuddin S, Hampton LL, Battey JF Jr,
Wilcox ER, Friedman TB, and Griffith AJ (2002) Dominant and recessive deafness caused
by mutations of a novel gene, TMC1, required for cochlear hair-cell function. Nature
Genetics. 30:277-84.
25.556
55
73.
Ahmed, Z.M., Smith, T.N., Riazuddin, S., Makishima, T., Ghosh, M., Bokhari, S., Menon,
S.N.P., Deshmukh, D., Griffith, A.J., Riazuddin, S., Friedman, T.B. and Wilcox, E.R. (2002)
Nonsyndromic recessive deafness DFNB18 and Usher Syndrome Type 1C are allelic
mutations of USH1C. Hum. Genet., 110: 527-531.
3.974
37
74.
Astuto, L.M., Bork, J.M., Weston, M.D., Askew J.W., Fields, R.R., Orten, D.J., Ohliger S.J.,
Riazuddin, S, Morell, R.J., Khan, S., Riazuddin, S., Kremer, H., Hauwe P.V., Moller C.G.,
Cremers C. W. R. J., Ayuso C. J., Heckenlively R., Rohrschneider K., Spandau U.,
Greenberg J., Ramesar R., Reardon W., Bitoun P., Millan J., Legge R., Friedman T.B., and
Kimberling W.J (2002). CDH23 Mutations and Phenotype Heterogeneity: Profile of 107
Diverse Usher Syndrome and Non-Syndromic Deafness. Am. J. Hum. Genet,. 71(2): 262275.
11.092
33
75.
Naz S, Giguere CM, Kohrman DC, Mitchem KL, Riazuddin S, Morell RJ, Ramesh A,
Srisailpathy S, Deshmukh D, Riazuddin S, Griffith AJ, Friedman TB, Smith RJH, Wilcox E
R (2002). Mutations in a Novel Gene, TIME, are associated with hearing loss linked to the
DFNB6 locus. Am. J. Hum. Genet., 71: 632-636.
11.092
20
76.
Chaudhry, G.R., Mateen, A., Kaskar, B., Bloda, M. and Riazuddin, S. (2002). Purification
and Biochemical Characterization of the Carbamate Hydrolase from Pseudomonas
sp.50432. Biotch. and App. Biochem., 36: 63-70.
1.903
4
77.
Park HJ, Shaukat S, Liu XZ, Hahn SH, Naz S, Ghosh M, Kim HN, Moon SK, Abe S,
Tukamoto K, Riazuddin S, Kabra M, Erdenetungalag R, Radnaabazar J, Khan S, Pandya
A, Usami SI, Nance WE, Wilcox ER, Riazuddin S, Griffith AJ (2003). Origins and
frequencies of SLC26A4 (PDS) mutations in east south Asians: global implications for the
epidemiology of deafness. J. Med. Genet., 40: 242-248.
5.535
42
78.
Ahmed, Z.M., Riazuddin, S., Riazuddin, S., Wilcox, E.R. (2003). The molecular genetics of
Usher Syndrome. Clinical Genet., 63: 431-444.
3.181
49
35
27
79.
Ahmed, Z.M, Morell, R.J., Riazuddin, S., Gropman, A., Shaukat, S., Ahmad, A.A., Mohiddin,
S.A., Fananapazir, L., Caruso, R.C., Husnain, T., Khan, S.N., Riazuddin, S., Griffith, A.J.,
Friedman, T.B. and Wilcox, E.R. (2003). Mutations of MYO6 are associated with recessive
deafness DFNB37; Am. J. Hum. Genet., 72(5): 1315-1322.
11.092
36
80.
Naz, S., Alasti, F., Mowjoodi, A., Riazuddin, S., Sanati, M.H., Friedman, T.B., Griffith, A.J.,
Wilcox, E.R., and Riazuddin, S. (2003). Distinctive audiometric profile associated with
DFNB21 alleles of TECTA. J. Med. Genet., 4: 360-363.
5.535
11
81.
Khan, S.N., Hasan, F., Sallaino, C., Perseu, L., and Riazuddin, S. (2003).
characterization of -Thalassemia in Pakistan. Hemoglobin, 27(3): 161-166.
Molecular
0.843
0
82.
Ahmed, Z.M., Riazuddin, S., Ahmad, J., Bernstein, S.L., Guo, Y., Sabar, M.F., Sieving, F.,
Riazuddin, S., Griffith, A.J., Friedman, T.B., Belyantseva, I.A., Wilcox, E.R. (2003).
PCDH15 is expressed in the neurosensory epithelium of the eye and ear and mutant alleles
are responsible for both USH1F and DFNB23. Hum. Mol. Genet., 12(24): 3215-3223.
7.806
55
83.
Naz S., Griffith A.J., Riazuddin, S., Hampton L.L., Battey J.F., Khan, S.N., Riazuddin, S.,
Wilcox E.R. and Friedman T.B. (2004). Mutations of ESPN Vestibular Dysfunction and
Autosomal Recessive Deafness. J. Med. Gent., 41: 591-595.
5.535
17
84.
Ahmed, Z.M., Li, X.C., Powell, S.D., Riazuddin, S., Young, T.L., Ramzan, K, Ahmad, Z.,
Luscombe, S., Khillon, K., MacLaren, L., Ploplis, B., Shotland, L.I., Ives, E., Riazuddin, S.,
Friedman, T.B., Morell, R.J. and Wilcox, E.R. (2004). Characterization of a new full length
TMPRSS3 isoform and identification of mutant alleles responsible for nonsyndromic
recessive deafness in Newfoundland and Pakistan. BMC Med. Genet., 5: 24.
2.65
85.
Rashid, B., Husnain,T., and Riazuddin, S. (2004). In-vitro shoot tip culture of cotton
(Gossypium Hirsutum) Pak. J. Bot., 36(4): 817-823.
0.290
86.
Zhang, Q., Zulfiqar, F., Riazuddin, S.A., Xiao, Xueshan, Ahmad, Z., Riazuddin, S.,
Hejtmancik, J.F. (2004). Autosomal recessive retinitis pigmentosa in a Pakistani family
mapped to CNG1 with identificatiuon of a novel mutation. Mol. Vision, 10: 884-9.
2.377
2
87.
Bashir, K., Husnain, T., Fatima, T., Latif, Z., Riaz, N., Mehdi, S. A. and Riazuddin, S.
(2004) Field evaluation and Risk Assessment of transgenic indica basmati Rice. Mol.
Breed., 13: 301-312.
2.357
5
309.870
1638
Sub-Total:
36
INTERNATIONAL PUBLICATIONS
DURING THE LAST FIVE YEARS (2005-2011)
DURING THE YEAR 2005
LIST OF INTERNATIONAL PUBLICATIONS BY
PROF. S. RIAZUDDIN
FOR THE YEAR TO 2005
S.No.
Publications
Impact
Factor
citati
on
1.
Ramzan, K., Shaikh, R.S., Ahmad, J., Khan, S.N., Riazuddin, S., Zubair, M.A.,
Friedman, T.B., Wilcox, E.R., Riazuddin, S. (2005). A new locus for
nonsyndromic deafness DFNB49 maps to chromosome 5q13.3-q14.1. Hum.
Genet., 116: 17-22.
4.523
5
2.
Ahmad, J., Khan S.N., Khan, S.Y., Ramzan, K, Riazuddin, S., Ahmed, Z.M.,
Wilcox, E.R., Friedman, T.B. and Riazuddin, S. (2005). DFNB48, a new
nonsyndromic recessive deafness locus maps to chromosome 15q23-q25.1.
Hum. Genet., 116: 407-412.
4.523
6
3.
Shaikh, R.S., Ramzan, K., Nazli, S., Sattar, S., Khan, S.N., Riazuddin, S.,
Ahmed, Z.M., Friedman, T.B., Wilcox, E.R., Riazuddin, S. (2005). A new locus
for nonsyndromic deafness DFNB51 maps to chromosome 11p13-p12. Am. J.
Med. Genet., 138A(4): 392-395.
2.063
4
4.
Riazuddin, S.A., Yasmeen, A., Zhang, Q., Yao, W., Sabar, M.F., Riazuddin, S.
and Hejtmanic, J.F. (2005). A new locus for autosomal recessive nuclear
cataract mapped to chromosome 19q13 in a Pakistani Family.
Invest.
Opthalmol. Vis. Sci. 46(2): 623-626.
3.431
18
5.
Riaz, N., Steinberb, S., Ahmad, J., Pluznikov, A., Riazuddin, S., Cox, N. and
Drayna, D. (2005). Genomewide significant linkage to stuttering on chromosome
12. Am. J. Hum. Genet. 76: 647-657.
12.303
14
6.
Zhang, Q., Zulfiqar, F., Xiao, X., Riazuddin, S.A., Ayyagari, Radha, Sabar, F.,
Caruso, R., Sieving, P.A., Riazuddin, S. and Hejtmancik, J.F. (2005). Severe
autosomal recessive Retinitis pigmentosa maps to chromosome 1p13.3-p21.2
between D1S2896 and D1S457 but outside ABCA4. Hum. Genet., 118: 356-65.
4.523
6
7.
Riazuddin, S.A., Zulfiqar, F., Zhang, Q., Yuri, V. Sergeev, Zaheeruddin, A,Q.,
Husnain, T., Caruso, R., Riazuddin, S., Sieving, P., and Hejtmancik, J.F. (2005).
Autosomal recessive Retinitis pigmentosa is associated with mutations in RP1 in
consanguineous Pakistani families. Invest. Opthalmol. Vis. Sci,. 46 (7): 22642270.
3.431
8
37
8.
Riazuddin, S.A., Yasmeen, A., Wenliang, Yao, Yuri, Qingjiong Zhang, Zulfiqar,
F., Riaz, A., Riazuddin, S., and Hejtmancik, J.F. (2005). Mutation in B3crystallin associated with autosomal recessive cataract in two Pakistani families.
Invest. Opthalmol. Vis. Sci., 46(6): 2100-2106.
3.431
32
9.
Zhang, Q., Zulfiqar, F., Xiao, X., Riazuddin, S.A., Xueshan, X., Yasmeen, A.,
Rogan, P.K., Caruso, R., Sieving, P.A., Riazuddin, S. and Hejtmancik, J.F.
(2005). A variant form of Oguchi disease mapped to 13q34 associated with
partial deletion of GRK1 gene. Mol. Vis. 11: 977-85
2.54
4
10.
Bashir, K., Husnain. T., Fatima, T., Latif, Z., Riaz, N. and Riazuddin, S. (2005)
Novel Indica Basmati Line (B-370) expressing two unrelated genes of Bacillus
thuringiensis is highly resistant to two lepidopteran insects in the field. Crop
Protection, 24(10): 870-879.
1.331
11
11.
Rafiq, M., Fatima T., Husnain, T., Bashir, K. and Riazuddin, S. (2005). Effect of
different media on callus formation and regeneration of different genotypes of
Miaze (Zea mays L.). Plant Tissue Culture, 15(1): 57-65.
1.271
0
43.37
108
TOTAL
38
LIST OF INTERNATIONAL PUBLICATIONS BY
PROF. S. RIAZUDDIN
FOR THE YEAR TO 2006
S.No.
Publications
Impact
Factor
citati
on
1.
Riazuddin, S., Khan, S.N., Ahmed, Z.M., Ghosh, M., Caution, K., Nazli, S.,
Kabra, M., Zafar, A.U., Chen, K., Naz, S., Antonellis, A., Pavan, W.J., Green,
E.D., Wilcox, E.R., Friedman, P.L., Morell, R.J., Riazuddin, S. and Friedman T.
B. (2006). Mutations in TRIOBP, which encodes a putative cytoskeletalorganizing protein, are associated with nonsyndromic recessive deafness. Am J.
Hum. Genet., 78: 137-43.
12.303
18
2.
Rao, A.Q., Hussain, S.S., Shahzad, M.S., Bokhari, S.Y, Raza, M.H, Rakha, A.,
Majeed, A., Shahid, A.A. Saleem, Z., Husnain, T. and Riazuddin, S. (2006)
Somatic embryogenesis in wild relatives of cotton (Gossypium Spp.). J.
Zhejiang. Univ. Sci. B. 7(4): 291-8
1.041
0
3.
Riaz, N., Husnain, T., Fatima, T., Makhdoom, R., Bashir, K., Masson, L.,
Altosaar, I. and Riazuddin, S. 2006. Development of Indica Basmati rice
harboring two insecticidal genes for sustainable resistance against lepidopteran
insects. South African Journal of Botany, 72(2): 217-223
1.080
4
4.
Riazuddin SA, Zulfiqar F, Zhang Q, Yao W, Li S, Jiao X, Shahzadi A, Amer M,
Iqbal M, Hussnain T, Sieving PA, Riazuddin S, and Hejtmancik JF. (2006).
Mutations in the gene encoding the alpha-subunit of rod phosphodiesterase in
consanguineous Pakistani families. Mol Vis. 12:1283-91.
2.54
2
5.
Rafiq, M., Fatima, T., Husnain, T., Bashir, K., Khan, M.A. and Riazuddin, S.
(2006). Regeneration and transformation of an elite inbred line of maize (Zea
mays L.), with a gene from Bacillus thuringiensis. South African Journal of
Botany, 72(3): 460-466.
1.080
0
6.
Ahmed ZM, Goodyear R, Riazuddin S, Lagziel A, Legan PK, Behra M, Burgess
SM, Lilley KS, Wilcox ER, Riazuddin S, Griffith AJ, Frolenkov GI, Belyantseva
IA, Richardson GP, and Friedman TB. (2006). The tip-link antigen, a protein
associated with the transduction complex of sensory hair cells, is protocadherin15. J Neurosci. 26(26):7022-34.
8.238
53
7.
Shabbir, M.I., Ahmed, Z.M., Khan, S.Y., Riazuddin, S., Waryah, A.M., Khan,
S.N., Camps, R.D., Ghosh, M., Kabra, M., Belyantseva, I.A., Friedman, T.B. and
Riazuddin, S. (2006). Mutations of human TMHS cause recessively inherited
nonsyndromic hearing loss. J. Med. Genet., 43(8): 634-40.
5.751
14
8.
Riazuddin, S., Ahmed, Z.M., Fanning, A.S., Lagziel, A., Kitajiri, S., Ramzan, K.,
Khan, S.N., Chattaraj, P., Friedman, P.L., Anderson, J.M., Belyantseva, I.A.,
Forge, A., Riazuddin, S., and Friedman, T.B. (2006) Tricellulin Is a TightJunction Protein Necessary for Hearing. Am. J. Hum. Genet., 79:1040-1051.
12.303
34
44.336
125
TOTAL
39
LIST OF INTERNATIONAL PUBLICATIONS BY
PROF. S. RIAZUDDIN
FOR THE YEAR TO 2007
S.No.
Publications
Impact
Factor
citati
on
1.
Khan, S.Y., Riazuddin, S., Tariq, M., Anwar, S., Shabbir, M.I., Riazuddin, S.A.,
Khan, S.N., Husnain, T., Ahmed, Z.M., Friedman, T.B. and Riazuddin, S.
(2007). Autosomal recessive nonsyndromic deafness locus DFNB63 at
chromosome 11q13.2–q13.3. Hum. Genet. 120(6): 789-793.
4.523
8
2.
Khan, S.Y., Ahmed, Z.M., Shabbir, M.I., Kitajiri, S., Kalsoom, S., Tasneem, S.,
Riazuddin, S., Khan, S.N., Friedman, T.B., Tariq, M., Riazuddin, A., Husnain, T.,
Riazuddin, S. (2007). Mutations in RDX encoding radixin cause nonsyndromic
hearing loss in humans. Hum. Mutat. 28(5): 417-423.
6.887
22
3.
Nal, N. Ahmed, Z.M., Erkal, E., Alper, O.M., Lu¨leci, G., Dinc, O., Waryah, A.M., Ain,
Q., Tasneem, S., Husnain, T., Chattaraj, P., Riazuddin, S., Boger, E., Ghosh, M.,
Kabra, M., Riazuddin, S., Morell, R.J. and Friedman, T.B. (2007). Mutational
spectrum of MYO15A: The large N-terminal extension of Myosin XVA is required for
hearing. Hum. Mutat., 28(10): 1014-19.
6.887
11
4.
Maqbool, A., Zahur, M., Irfan, M., Qaiser, U., Rashid, B., Husnain, T. and
Riazuddin, S. (2007). Identification, characterization and expression of drought
related alpha-crystalline heat shock protein gene (GHSP26) from desi cotton.
Crop Sci., 47(6): 2437-2444.
1.735
2
5.
Zhang, Q., Zulfiqar, F., Xiao, X., Riazuddin, S.A., Ahmad, Z., Caruso, R.,
MacDonald, I., Sieving, P., Riazuddin, S. and Hejtmancik, J.F. (2007) Severe
retinitis pigmentosa mapped to 4p15 and associated with a novel mutation in the
PROM1 gene. Hum. Genet., 122(3-4): 293-9.
4.523
15
6.
Sharif, S., Nakagawa ,T., Ohno, T., Matsumoto, M., Kita, T., Riazuddin, S. and
Ito, J.. (2007) The potential use of bone marrow stromal cells for cochlear cell
therapy. Neuroreport, 18(4):351-4.
1.805
4
7.
Ain, Q., Nazli, S., Riazuddin, S., Jaleel, A.U., Riazuddin, S.A., Zafar, A.U., Khan,
S.HN., Husnain T., Griffith, A.J., Ahmad. Z.M., Friedman. T.B. and Riazuddin, S.
(2007). The autosomal recessive nonsyndromic deafness locus DFNB72 is
located on chromosome 19p13.3. Hum. Genet., 122(5): 445-450.
4.523
2
8.
Rahman, M., Rashid, H., Shahid, A. A., Bashir, K., Husnain, T. and Riazuddin, S.
(2007). Insect resistance and risk assessment studies of advanced generations
of Basmati rice expressing two genes of Bacillus thuringiensis (2007). Electro. J.
Biotech. 10(2): 240-251.
2.881
0
9.
Nasir, I.A. and Riazuddin, S. (2007). New approaches to generate diseaseresistant Gladiolus. World J. Micro. Biotech., 24(3): 367-373
1.082
0
10.
Kitajiri, S.I., McNamara, R., Makishima, T., Husnain, T., Zafar, A.U., Kittles, R.A.,
Ahmed, Z.M., Friedman, T.B., Riazuddin, S. and Griffith A.J. (2007). Identities,
frequencies and origins of TMC1 mutations causing DFNB7/B11 deafness in
3.304
10
40
Pakistan. Clin. Genet. 72: 546-550.
11.
Butt, T., Yao. W., Kaul. H., Xiaodong. J., Gradstein. L., Zhang. Y., Husnain, T.,
Riazuddin, S., Hejtmancik . F. J and Riazuddin. S.A. (2007) Localization of
autosomal recessive congenital cataracts in consanguineous Pakistani families
to a new locus on chromosome 1p. Mol. Vis., 13:1635-1640.
2.54
6
12.
Verma, I.C., Kleanthous, M., Saxena, R., Fucharoen, S., Winichagoon, P.,
Riazuddin, S., Khan, S.N., Akbari, M.T., Izadyar, M., Kotea, N., Old, J.M.,
Ioannou, P.A., and Khan, B. (2007). Multicenter Study of the Molecular Basis of
Thalassemia Intermedia in Different Ethnic Populations. Hemoglobin, 31(4):
439-452.
1.129
6
41.819
86
TOTAL
41
LIST OF INTERNATIONAL PUBLICATIONS BY
PROF. S. RIAZUDDIN
FOR THE YEAR TO 2008
S.No.
Publications
Impact
Factor
citati
on
1.
Pasha, Z., Wang, Y., Riazuddin, S., Zhang, D., Zhao, T. and Ashraf, M. (2008).
Preconditioning enhances cell survival and differentiation of stem cells during
transplantation in infarcted myocardium. Cardiovascular Res., 77(1): 134-142.
5.801
42
2.
Riazuddin, S., Nazli, S., Ahmed, Z.M., Yang, Y., Zulfiqar, F., Shaikh, R.S., Zafar,
A.U., Khan, S.N., Sabar, M.F., Javid, F.T., Wilcox, E.R., Ekaterini, T., Boger,
E.T., Sellers, J.R., Belyantseva, I.A., Riazuddin, S. and Friedman T. (2008).
Mutation Spectrum of MYO7A and Evaluation of a Novel Nonsyndromic
Deafness DFNB2 Allele with Residual Function. Hum. Mutat., 29(4): 502-511.
6.887
12
3.
Collin, R.W.J., Kalay, E., Tariq, M., Peters, T., Zwaag, B van der., Venselaar, H.,
Oostrik, J., Lee, K.M., Ahmed, Z.M., Caylan, R., Li, Y., Spierenburg, H.A.,
Eyupoglu, E., Heister, A., Riazuddin, S., Bahat, E., Ansar, M., Arslan, S.,
Wollnik, B., Brunner, H., Cremers, Cor W.R.J., Karaguzel, A., Ahmad, W.,
Cremers, F.P.M. Vriend, G., Friedman, T.B., Riazuddin, S., Leal, S.M. and
Kremer, H. (2008). Mutations of ESRRB encoding estrogen-related receptor beta
cause autosomal recessive nonsyndromic hearing impairment DFNB35. Am. J.
Hum. Genet. 83(1): 25-138
12.303
12
4.
Ahmed, Z.M., Riazuddin, S., Aye, S., Ali, R.A., Venselaar, H., Anwar, S.,
Belyantseva, P.P., Qasim,M., Riazuddin, S. and Friedman, T.B. (2008). Gene
structure and mutant alleles of PCDH15: nonsyndromic deafness DFNB23 and
type 1 Usher syndrome. Hum. Genet. 124(3):215-23.
4.523
6
5.
Mahmood-Ur-Rahman, Ali, I., Husnain, T. and Riazuddin, S. (2008) RNA
interference: The story of gene silencing in plants and humans. Biotechnol.
Adv., 26(3): 202-9.
8.250
0
6.
Ahmed, Z.M., Masmoudi, S., Kalay, E., Belyantseva, I.A., Mosrati, M.A., Collin,
R.W., Riazuddin, S., Hmani-Aifa, M., Venselaar, H., Kawar, M.N., Tlili, A., van
der Zwaag, B., Khan, S.Y., Ayadi, L., Riazuddin, S.A., Morell, R.J., Griffith, A.J.,
Charfedine, I., Caylan, R., Oostrik, J., Karaguzel, A., Ghorbel, A., Riazuddin, S.,
Friedman, T.B., Ayadi, H. and Kremer, H. (2008). Mutations of LRTOMT, a
fusion gene with alternative reading frames, cause nonsyndromic deafness in
humans. Nat. Genet., 40(11):1335-40.
34.284
7
7.
Idrees, M., Riazuddin, S. (2008) Frequency distribution of hepatitis C virus
genotypes in different geographical regions of Pakistan and their possible routes
of transmission. BMC. Infect. Dis. 23;8:69.
2.55
12
8.
Firasat, S., Riazuddin, S.A., Khan, S.N., Riazuddin S. (2008) Novel CYP1B1
mutations in consanguineous Pakistani families with primary congenital
glaucoma. Mol. Vis. 14:2002-09
2.54
0
42
9.
Firasat, S., Riazuddin, S.A., Hejtmancik, J.F., and Riazuddin, S. (2008) Primary
congenital glaucoma localizes to chromosome 14q24.2-24.3 in two
consanguineous Pakistani families. Mol. Vis. 14:1659-65.
2.54
3
10.
Tariq, M.A., Sabir, M.F., Riazuddin, S.A., and Riazuddin, S. (2008) Haplotype
analysis of two X-chromosome STR clusters in the Pakistani population. Int. J.
Legal Med. 123(1):85-7.
2.793
1
11.
Khan Barozai MY, Irfan, M., Yousaf, R., Ali, I., Qaisar, U., Maqbool, A., Zahoor,
M., Rashid, B., Hussnain, T., and Riazuddin S. (2008) Identification of microRNAs in cotton. Plant Physiol. Biochem., 46(8-9):739-51.
2.485
5
12.
Tariq MA, Ullah O, Riazuddin SA, Riazuddin S. (2008) Allele frequency
distribution of 13 X-chromosomal STR loci in Pakistani population. Int. J. Legal
Med., 122(6):525-8.
2.793
4
13.
Nasir IA, Riazuddin S. (2008) In vitro selection for Fusarium wilt resistance in
Gladiolus. J. Integr. Plant Biol., 50(5):601-12.
1.395
2
14.
Rashid B, Saleem Z, Husnain T, Riazuddin S. (2008). Transformation and
Inheritance of Bt Genes in Gossypium hirsutum. J. Plant Bio. 51(4): 248-254.
0.785
0
15.
Maqbool A, Zahur M, Husnain T and Riazuddin S (2008). GUSP1 and GUSP2,
two droughts – responsive genes in Gossypium arboretum have homology to
universal stress Proteins. Plant Mol. Biol Rep
3.847
1
16.
Zahur M, Maqbool A, Irfan M, Barozai MYK, Rashid B, Husnain T. and
Riazuddin, S. (2008). Isolation and Functional Analysis of Cotton Universal
Stress Protein Promoter in Response to Phytohormones and Abiotic Stresses.
Mol. Biol. 43(4):628-635.
0.805
0
17.
Maqbool, A., Zahur, M., Irfan, M., Barozai, M.Y.K., Rashid, B., Husnain, T and
Riazuddin, S. (2008). Identification and Expression of six drought responsive
transcripts through Differential Display in desi cotton (Gossypium arboreum).
Mol. Biol. 42(4):492-498.
0.805
1
18.
Irfan, M., Barozai, M.Y.K., Maqbool, A., Qaiser, U., Zahur, M., Rashid, B.,
Hernandez, A.G., Bohnert, H.J., Husnain, T and Riazuddin, S. (2008). Drought
stressed cDNA library from leaves of Gossypium arboreum. GenBank accession
number FL576377- FL577777 (1401 EST’s).
N.A
0
19.
Shahid A.A, Husnain T and Riazuddin S (2008). Ascochyta blight of chickpea:
Production of phytotoxins and disease management. Biotech Adv. 211-515
8.250
0
103.636
108
TOTAL
43
LIST OF INTERNATIONAL PUBLICATIONS BY
PROF. S. RIAZUDDIN
FOR THE YEAR TO 2009
S.No.
Publications
Impact
Factor
citati
on
1.
Anwar S, Riazuddin S, Ahmed ZM, Tasneem S, Jaleel AU, Han SY, Griffith AJ,
Friedman TB and Riazuddin S. (2009) SLC26A4 mutation spectrum associated
with DFNB4 deafness and Pendred syndrome in Pakistan. J. Hum. Genet.,
54(5):266-70.
2.547
2
2.
Hmani-Aifa M, Benzina Z, Zulfiqar F, Dhouib H, Shahzadi A, Ghorbel A, Rebaï A,
Söderkvist P, Riazuddin S, Kimberling WJ, and Ayadi H. (2009) Identification of
two new mutations in the GPR98 and the PDE6B genes segregating in a
Tunisian family. Eur. J. Hum. Genet., 17(4):474-82.
3.564
3
3.
Ahmed, Z.M., Riazuddin, S., Khan, S., Friedman, P., Riazuddin, S. and
Friedman, T. (2009). USH1H, a novel locus for type I Usher syndrome, maps to
chromosome 15q22-23. Clin. Genet., 75(1):86-91.
3.304
6
4.
Zahur, M., Maqbool, A., Irfan, M., Barozai, M.Y., Qaiser, U., Rashid, B., Husnain,
T., and Riazuddin, S. (2009) Functional analysis of cotton small heat shock
protein promoter region in response to abiotic stresses in tobacco using
Agrobacterium-mediated transient assay. Mol. Biol. Rep., 36(7):1915-21.
2.038
0
5.
Choi, B.Y., Ahmed, Z.M., Riazuddin, S., Bhinder, M.A., Shahzad, M., Husnain,
T., Riazuddin S., Griffith, A.J. and Friedman, T.B. (2009). Identities and
frequencies of mutations of the otoferlin gene (OTOF) causing DFNB9 deafness
in Pakistan. Clin. Genet., 75(3):237-43
3.304
5
6.
Waryah, A.M., Rehman, A.U., Ahmed, Z.M., Bashir, Z.H., Khan, S.Y., Zafar,
A.U., Friedman, T.B., Riazuddin, S., and Riazuddin S. (2009) DFNB74, a new
autosomal recessive nonsyndromic hearing impairment locus at chromosome
12q14.2-q15. Clin. Genet., 76(3):270-5.
3.304
0
7.
Ali, M., Mckibbin, M., Booth, A., Parry, D.A., Jain, P., Riazuddin, S.A.,
Hejtmancik, J.F., Khan, S.N., Firasat, S., Shires, M., Gilmour, D.F., Towns, K.,
Murphy, A., Azmanov, D., Tournev, I., Cherninkova, S., Jafri, H., Raashid, Y.,
Toomes, C., Craig, J., Mackey, D.A., Kalaydjieva, L., Riazuddin, S., and
Inglehearn, C.F. (2009) Null mutations in ltbp2 cause primary congenital
glaucoma. Am. J. Hum. Genet., 84(5):664-71.
12.303
12
8.
Zahur, M., Maqbool, A., Irfan, M., Barozai, M.Y.K., Qaiser, U., Rashid, B.,
Husnain, T., and Riazuddin, S. (2009). Functional analysis of cotton small heat
shock protein promoter region in response to abiotic stresses in tobacco Using
Agrobacterium-Mediated transient assay. Mol. Biol. Rep., 36(7):1915-21.
2.038
0
9.
Riazuddin, S.A., Kaul, H., Butt, T., and Riazuddin, S. (2009) Novel SIL1
mutations in consanguineous Pakistani families mapping to chromosome 5q31.
Mol. Vis.15:1050-6.
2.54
0
44
10.
Idrees, M., Riazuddin, S. (2009). A study of best positive predictors for
sustained virologic response to interferon alpha plus ribavirin therapy in naive
chronic hepatitis C patients. BMC Gastroenterol., 9:5.
1.89
2
11.
Schultz, J.M., Khan, S.N., Ahmed, Z.M., Riazuddin, S., Waryah, A.M. Chhatre,
D., Starost, M.F., Ploplis, B., Buckley, S., Velasquez, D., Kabra, M., Lee, K.,
Hassan, M.J., Ali, G., Ansar, M., Ghosh, M., Wilcox, E.R., Ahmad, W., Merlino,
G., Leal, S.M., Riazuddin, S., Friedman, T.B., and Morell, R.J. (2009). Mutations
affecting regulation of the HGF gene are the cause of nonsyndromic hearing loss
DFNB39. Am. J. Hum. Genet., 85(1):25-39.
12.303
3
12.
Riazuddin, S., Anwar, S., Fischer, M., Ahmed, Z.M., Khan, S.Y., Janssen, A.G.,
Zafar, A.U., Scholl, U., Husnain, T., Belyantseva, I.A., Friedman, P.L.,
Riazuddin S., Friedman, T.B., and Fahlke, C. (2009). Molecular basis of
DFNB73: mutations of BSND can cause nonsyndromic deafness or Bartter
syndrome. Am. J. Hum. Genet., 85(2):273-80.
12.303
2
13.
Idrees, M., Butt, S., Awan, Z., Aftab, M., Khubaib, B., Rehman, I.U., Akram, M.,
Manzoor, S., Akbar, H., Rafiqe, S., and Riazuddin S. (2009). Nucleotide identity
and variability among different Pakistani hepatitis C virus isolates. Virol J, 6:130.
2.44
0
14.
Zaidi, M.A., Ye, G., Yao, H., You, T.H., Loit, E., Dean, D.H., Riazuddin, S., and
Altosaar, I. (2009). Transgenic rice plants expressing a modified cry1Ca1 gene
are resistant to Spodoptera litura and Chilo suppressalis. Mol. Biotechnol.,
43(3):232-42.
2.44
1
15.
Idrees, M., Rafique, S., Rehman, I., Akbar, H., Yousaf, M.Z., Butt, S., Awan, Z.,
Manzoor, S., Akram, M., Aftab, M., Khubaib, B., and Riazuddin, S. (2009).
Hepatitis C virus genotype 3a infection and hepatocellular carcinoma: Pakistan
experience. World J. Gastroenterol., 15(40):5080-5.
2.092
2
16.
Hussain, S.S., Rao, A.Q., Husnain, T., and Riazuddin, S. (2009). Cotton
somatic embryo morphology affects its conversion to plant. Biolog. Plant, 53
(2): 307-311
17.
Rao, A.Q., Bakhsh, A., Kiani, S, Shahzad, K., Shahid, A.A., Husnain T. and
Riazuddin S. (2009) The Myth of Plant Transformation. Biotechnol. Adv,
27(6):753-63.
TOTAL
1.656
3
8.250
3
78.316
44
45
LIST OF INTERNATIONAL PUBLICATIONS BY
PROF. S. RIAZUDDIN
FOR THE YEAR TO 2010
S.No.
Publications
Impact
Factor
citati
on
1.
Maqbool, A., Abbas, W., Rao, A.Q., Irfan, M., Zahur, M., Bakhsh, A., Riazuddin,
S., and Husnain, T. (2010). Gossypium arboreum GHSP26 enhances drought
tolerance in Gossypium hirsutum. Biotechnol. Prog. 26(1):21-5.
2.398
0
2.
Khan, S.Y., Riazuddin, S., Shahzad, M., Ahmed, N., Zafar, A.U., Rehman, A.U.,
Morell, R.J., Griffith, A.J., Ahmed, Z.M., Riazuddin, S., and Friedman, T.B.
(2010) DFNB79: reincarnation of a nonsyndromic deafness locus on
chromosome 9q34.3. Eur. J. Hum. Genet., 18(1):125-129.
3.564
1
3.
Khaliq, S., Khaliq, S.A., Zahur, M., Ijaz, B., Jahan, S., Ansar, M., Riazuddin, S.,
and Hassan, S. (2010). RNAi as a new therapeutic strategy against HCV.
Biotechnol. Adv. 28(1):27-34.
8.250
2
4.
Naz S, Riazuddin SA, Li L, Shahid M, Kousar S, Sieving PA, Hejtmancik JF, and
Riazuddin S. (2010). A novel locus for autosomal recessive retinitis pigmentosa
in a consanguineous Pakistani family maps to chromosome 2p. Am J
Ophthalmol. 149(5):861-6.
3.833
1
5.
Shehzadi, A., Riazuddin, S.A., Ali, S., Li, D., Sabar, M.F., Khan, S.N., Sieving,
P.A., Hejtmancik, J.F. and Riazuddin, S. (2010). Nonsense mutation of MERTK
causes autosomal recessive retinitis pigmentosa in a consanguineous Pakistani
family. Br J Ophthalmol. 94(8):1094-9.
2.917
0
6.
Kang, C., Riazuddin, S., Mundorff, J., Krasnewich, D., Friedman, P.L., Mullikan,
J.C., and Drayna, D. (2010) Association of persistent stuttering with mutations in
the lysosomal enzyme targeting pathway. N Engl J Med. 362(8):677-85.
47.050
9
7.
Rehman, A.U., Morell, R.J., Khan, S.Y., Belyantseva, I.A., Boger, E.T., Shahzad,
M., Ahmed, Z.M., Riazuddin, S., Khan S.N., Riazuddin, S., and Friedman, T.B.
(2010) Targeted Capture and Next-Generation Sequencing of the 2.9 Megabase
DFNB79 Locus Identifies Mutations in C9orf75, Encoding TAPERIN, as a Cause
of Nonsyndromic Deafness. Am J Hum Genet. 86(3):378-88.
12.303
5
8.
Yasmeen A, Riazuddin SA, Kaul H, Mohsin S, Khan M, Qazi ZA, Nasir IA, Zafar
AU, Khan SN, Husnain T, Akram J, Hejtmancik JF, and Riazuddin S. (2010).
Autosomal recessive congenital cataract in consanguineous Pakistani families is
associated with mutations in GALK1. Mol Vis.16:682-8.
2.54
0
9.
Hertzano, R., Puligilla, C., Chan, S.L., Timothy, C., Depireux, D., Wolf, J.,
Ahmed, Z.M., Friedman, T.B., Riazuddin, S., Kelley, M.W. and Strome, S.E.
(2010). CD44 is a marker for the outer pillar cell in the early post-natal mouse
inner ear. J Assoc Res Otolaryngol. 11(3):407-18.
2.436
0
10.
Kitajiri, S., Sakamoto, T., Belyantseva, I.A., Goodyear, R.J., Stepanyan, R.,
Fujiwara, I., Bird, J.E., Riazuddin, S., Riazuddin, S., Ahmed, Z.M., Hinshaw,
J.E., Sellers, J., Bartles, J.R., Hammer, J.A., Richardson, G.P., Griffith, A.J.,
31.152
1
46
Frolenkov, G.I., and Friedman, T.B. (2010). Actin-Bundling Protein TRIOBP
Forms Resilient Rootlets of Hair Cell Stereocilia Essential for Hearing. Cell.
141(5):786-98.
11.
Riazuddin SA, Iqbal M, Wang Y, Masuda T, Chen Y, Bowne S, Sullivan LS,
Waseem NH, Bhattacharya S, Daiger SP, Zhang K, Khan SN, Riazuddin S,
Hejtmancik JF, Sieving PA, Zack DJ, Katsanis N. (2010) A splice-site mutation in
a retina-specific exon of BBS8 causes nonsyndromic retinitis pigmentosa. Am J
Hum Genet. 86(5):805-12.
12.303
1
12.
Kaul H, Riazuddin SA, Shahid M, Kousar S, Butt NH, Zafar AU, Khan SN,
Husnain T, Akram J, Hejtmancik JF, and Riazuddin S. (2010). Autosomal
recessive congenital cataract linked to EPHA2 in a consanguineous Pakistani
family. Mol Vis. 16:511-7.
2.54
4
13.
Kaul H, Riazuddin SA, Yasmeen A, Mohsin S, Khan M, Nasir IA, Khan SN,
Husnain T, Akram J, Hejtmancik JF, and Riazuddin S. (2010). A new locus for
autosomal recessive congenital cataract identified in a Pakistani family. Mol Vis.
16:240-5.
2.54
4
14.
Khan, M., Mohsin, S., Khan, S.N., and Riazuddin, S. (2010) Repair of senescent
myocardium by mesenchymal stem cells is dependent on the age of donor mice.
J. Cell. Mol. Med. Available online
5.228
0
15.
Khan M, Akhtar S, Mohsin S, N Khan S, and Riazuddin S. (2010). Growth
Factor Preconditioning Increases the Function of Diabetes-Impaired
Mesenchymal Stem Cells. Stem Cells Dev. Available online
4.146
0
16.
Raza MH, Riazuddin S, Drayna D. (2010). Identification of an autosomal
recessive stuttering locus on chromosome 3q13.2-3q13.33. Hum Genet.
128(4):461-3.
4.523
0
17.
Riazuddin SA, Shahzadi A, Zeitz C, Ahmed ZM, Ayyagari R, Chavali VR,
Ponferrada VG, Audo I, Michiels C, Lancelot ME, Nasir IA, Zafar AU, Khan SN,
Husnain T, Jiao X, MacDonald IM, Riazuddin S, Sieving PA, Katsanis N, and
Hejtmancik JF. (2010). A mutation in SLC24A1 implicated in autosomalrecessive congenital stationary night blindness. Am J Hum Genet. 87(4):523-31.
12.303
0
18.
Li L, Nakaya N, Chavali VR, Ma Z, Jiao X, Sieving PA, Riazuddin S, Tomarev
SI, Ayyagari R, Riazuddin SA, and Hejtmancik JF. (2010). A mutation in
ZNF513, a putative regulator of photoreceptor development, causes autosomalrecessive retinitis pigmentosa. Am J Hum Genet. 87(3):400-9.
12.303
0
19.
Kaul H, Riazuddin SA, Qazi ZA, Nasir IA, Zafar AU, Khan SN, Husnain T, Akram
J, Hejtmancik JF, and Riazuddin S. (2010). Ectopia lentis in a consanguineous
pakistani family and a novel locus on chromosome 8q. Arch Ophthalmol.;
128(8):1046-9.
3.86
0
20.
Khan, M., Mohsin, S., Khan, S.N. and Riazuddin S, (2010). Lin-c-kit+BM-derived
stem cells repair Infarcted Heart. Journal of Stem Cells Regenerative
Medicine 16: 15-25.
176.189
28
TOTAL
47
LIST OF INTERNATIONAL PUBLICATIONS BY
PROF. S. RIAZUDDIN
FOR THE YEAR TO 2011
S.No.
Publications
Impact
Factor
citati
on
1.
Mohsin S, Shams S, Ali Nasir G, Khan M, Javaid Awan S, Khan SN, and
Riazuddin S. (2011) Enhanced hepatic differentiation of mesenchymal stem
cells after pretreatment with injured liver tissue. Differentiation. 81(1):42-8.
3.311
0
2.
Ahmed ZM, Yousaf R, Lee BC, Khan SN, Lee S, Lee K, Husnain T, Rehman AU,
Bonneux, S, Ansar M, Ahmad W, Leal SM, Gladyshev VN, Belyantseva IA, Van
Camp G, Riazuddin S, and Friedman TB, Riazuddin S. (2011) Functional null
mutations of MSRB3 encoding methionine sulfoxide reductase are associated
with human deafness DFNB74. Am J Hum Genet. 88: 19-29.
12.303
0
3.
Borck G, Rehman AU, Lee K, Pogoda HM, Kakar N, von Ameln S, Grillet N,
Hildebrand MS, Ahmed ZM, Nürnberg G, Ansar M, Basit S, Javed Q, Morell RJ,
Nasreen N, Shearer AE, Ahmad A, Kahrizi K, Shaikh RS, Ali RA, Khan SN,
Goebel I, Meyer NC, Kimberling WJ, Webster JA, Stephan DA, Schiller M, Bahlo
M, Najmabadi H, Gillespie PG, Nürnberg P, Wollnik B, Riazuddin S, Smith RJH,
Ahmad W, Müller U, Hammerschmidt M, Friedman TB, Riazuddin S, Leal SM,
Ahmad J, Kubisch C. (2011) Loss-of-function mutations of ILDR1 cause
autosomal recessive hearing impairment DFNB42. Am J Hum Genet. 88: 127137.
Riazuddin S, Ahmed ZM, Hegde RS, Khan SN, Nasir I, Shaukat U, Riazuddin S,
Butman JA, Griffith AJ, Friedman TB, and Choi BY. (2011) Variable expressivity
of FGF3 mutations associated with deafness and LAMM syndrome. BMC Med
Genet . 12(1):21.
12.303
1
2.84
0
5.
Rehman AU, Gul K, Morell RJ, Lee K, Ahmed ZM, Riazuddin S, Ali RA, Shahzad
M, Jaleel AU, Andrade PB, Khan SN, Khan S, Brewer CC, Ahmad W, Leal SM,
Riazuddin S, Friedman TB. Mutations of GIPC3 cause nonsyndromic hearing
loss DFNB72 but not DFNB81 that also maps to chromosome 19p. Hum Genet
(2011). Available online.
5.047
0
6.
Waryah AM, Ahmed ZM, Bhinder MA, Choo DI, Sisk RA, Shahzad M, Khan SN,
Friedman TB, Riazuddin S, Riazuddin S. Molecular and Clinical studies of Xlinked deafness among Pakistani families. J Hum Genet (2011). 56(7): 534-540.
2.496
0
7.
Ali RA, Rehman AU, Khan SN, Husnain T, Riazuddin S, Friedman TB, Ahmed
ZM, Riazuddin S. DFNB86, A novel autosomal recessive nonsyndromic
deafness locus on chromosome 16p13.3. Clin Genet (2011). In press.
2.942
8.
Schultz JM, Bhatti R, Madeo AC, Turriff A, Muskett JA, Zalewski CK, King KA,
Ahmed ZM, Riazuddin S, Ahmad N, Hussain Z, Qasim M, Khan SN, Meltzer MR,
Liu XZ, Munisamy M, Ghosh M, Rehm HL, Tsilou EK, Griffith AJ, Zein WM,
Brewer CC, Riazuddin S, Friedman TB. Allelic hierarchy of CDH23 mutations
causing nonsyndromic deafness DFNB12 or Usher syndrome USH1D in
compound heterozygotes. J Med Genet (2011). In press.
7.037
9.
Iqbal M, Naeem MA, Riazuddin SA, Ali S, Farooq T, Qazi ZA, Khan SN, Husnain
T, Riazuddin S, Sieving PA, Hejtmancik JF, Riazuddin S. Association of
Pathogenic Mutations in TULP1 With Retinitis Pigmentosa in Consanguineous
Pakistani Families. Arch Ophthalmol (2011). 129:1351-1357.
3.52
4.
48
0
10.
Ashfaq UA, Masoud MS, Nawaz Z, Riazuddin S. Glycyrrhizin as antiviral agent
against Hepatitis C Virus. J Transl Med (2011). 9:112.
3.51
0
11.
Ali S, Riazuddin SA, Shahzadi A, Nasir IA, Khan SN, Husnain T, Akram J,
Sieving PA, Hejtmancik JF, Riazuddin S. Mutations in the β-subunit of rod
phosphodiesterase identified in consanguineous Pakistani families with
autosomal recessive retinitis pigmentosa. Mol Vision (2011). 17:1373-1380.
2.54
0
12.
Chen J, Smaoui N, Hammer MB, Jiao X, Riazuddin SA, Harper S, Katsanis N,
Riazuddin S, Chaabouni H, Berson EL, Hejtmancik JF. Molecular analysis of
Bardet-Biedl syndrome families: report of 21 novel mutations in 10 genes. IOVS
(2011). 52:5317-5324.
3.466
0
13.
Chen J, Ma Z, Jiao X, Fariss R, Kantorow WL, Kantorow M, Pras E, Frydman M,
Pras E, Riazuddin S, Riazuddin SA, Hejtmancik JF. Mutations in FYCO1 cause
autosomal-recessive congenital cataracts. Am J Hum Genet (2011). 88:627638.
11.680
2
14.
Ashfaq UA, Masoud MS, Khaliq S, Nawaz Z, Riazuddin S. Inhibition of hepatitis
C virus 3a genotype entry through Glanthus Nivalis Agglutinin. Virol J (2011).
8:248.
Rehman S, Ashfaq UA, Riaz S, Javed T, Riazuddin S. Antiviral activity of Acacia
nilotica against Hepatitis C Virus in liver infected cells. Virol J (2011). 8:220.
2.55
0
2.55
0
16.
Ansar M, Ashfaq UA, Shahid I, Sarwar MT, Javed T, Rehman S, Hassan S,
Riazuddin S. Inhibition of full length hepatitis C virus particles of 1a genotype
through small interference RNA. Virol J (2011). 8:203.
2.55
0
17.
Ashfaq UA, Javed T, Rehman S, Nawaz Z, Riazuddin S. Lysosomotropic agents
as HCV entry inhibitors. Virol J (2011). 8:163.
Ashfaq UA, Javed T, Rehman S, Nawaz Z, Riazuddin S. An overview of HCV
molecular biology, replication and immune responses. Virol J (2011). 8:161.
2.55
0
2.55
0
Ashfaq UA, Khan SN, Nawaz Z, Riazuddin S. In-vitro model systems to study
Hepatitis C Virus. Genet Vaccines (2011). 9:7.
Rao AQ, Irfan M, Saleem Z, Nasir IA, Riazuddin S, Husnain T. Overexpression
of the phytochrome B gene from Arabidopsis thaliana increases plant growth and
yield of cotton (Gossypium hirsutum). J Zhejiang Univ Sci B (2011). 12:326334.
Ashfaq UA, Javed T, Rehman S, Nawaz Z, Riazuddin S. Inhibition of HCV 3a
core gene through Silymarin and its fractions. Virol J (2011). 8:153.
2.10
0
0.322
0
2.55
1
15.
18.
19.
20.
21.
22.
Naz S, Ali S, Riazuddin SA, Farooq T, Butt NH, Zafar AU, Khan SN, Husnain T,
Macdonald IM, Sieving PA, Hejtmancik JF, Riazuddin S. Mutations in RLBP1
associated with fundus albipunctatus in consanguineous Pakistani families. Br J
Ophthalmol (2011). 95:1019-1024.
2.934
0
23.
Ali Ashfaq U, Ansar M, Sarwar MT, Javed T, Rehman S, Riazuddin S. Post2.55
transcriptional inhibition of hepatitis C virus replication through small interference
RNA. Virol J (2011). 8:112.
Javed T, Ashfaq UA, Riaz S, Rehman S, Riazuddin S. In-vitro antiviral activity of
2.55
Solanum nigrum against Hepatitis C Virus. Virol J (2011). 8:26.
TOTAL (2011)
98.751
0
24.
49
0
4
LIST OF INTERNATIONAL PUBLICATIONS BY
PROF. S. RIAZUDDIN
FOR THE YEAR TO 2012
S.No.
Publications
1.
Choudhery, M.S., Khan. M., Mahmood, R., Mehmood, A., Khan, S.N. and
Riazuddin, S. (2012) Bone marrow derived mesenchymal stem cells from aged
mice have reduced wound healing, angiogenesis, proliferation and antiapoptosis Capabilities. Cell Biol. Inter. Manuscript No. CBI20110183
1.8
2.
Choudhery, M.S., Khan, M., Mahmood, R., Mohsin, S., Akhtar, S., Ali, F., Khan,
S.N., and Riazuddin, S. (2012) Mesenchymal stem cells conditioned with
glucose depletion augments their ability to repair-infarcted myocardium. J. Cell.
Mol. Med. Vol XX, No X, pp.1-12.
4.6
3.
Ali, G., Mohsin, S., Nasir, G.A., Shams, S., Khan, N.S., and Riazuddin, S. (2012)
Nitric oxide augments mesenchymal stem cell ability to repair liver fibrosis.
Submitted to Translational Medicine
TOTAL (2012)
3.5
GRAND TOTAL
Impact
Factor
citati
on
9.9
906.187
50
499
LIST OF CHAPTERS IN THE BOOKS WRITTEN/ EDITED
BY PROF. S. RIAZUDDIN
TITLE OF THE BOOK/ CHAPTER/ PUBLISHER
1.
Riazuddin, S. (1980). Purification and properties of pyrimidine dimer specific endonucleases from Micrococcus luteus.
Methods Enzymol., 65: 185-191.
2.
Riazuddin, S. (1980). Purification and properties of an endonuclease specific for non-pyrimidine dimer damage induced
by ultraviolet radiations. Methods Enzymol. 65: 231-235.
3.
Riazuddin, S. (1980). Purification and properties of a 3-methyladenine DNA glycosylase from Escherchia coli. Methods
Enzymol., 65: 290-295.
4.
Khan, A.M., Riazuddin, S., Qadir, A., Qazi, M.N. Physics and Contemporary Needs. Vol. 6. Plenum Press, New York
(1984).
5.
Riazuddin, S. Base modifications and their repair. In: "Repairable lesions in microorganisms," Eds: J. E. Hurst, J. Dillon
and A. Nasim, pp. 149-186, Plenum Press, New York (1984).
6.
Riazuddin, S. Establishment of International Centre for Genetic Engineering and Biotechnology. UNIDO-IS.254.
7.
Riazuddin, S. IARC Monograph on the Evaluation of Carcinogenic Risks of Chemicals to Humans. Vol. 31. (1982).
8.
Riazuddin, S. Centre for the production of enzymes. UNIDO-IS.271.
9.
Riazuddin, S. Capability building in biotechnology and genetic engineering in developing countries. UNIDO-IS.608.
10.
Grossman, L., Riazuddin, S. Doniger, J. and Hamilton, L. (1977). DNA synthesis: Present and future "NATO Advanced
study Institutes Series." Eds.: Molineuse, I. and Kohiyama, M., Plenum Press. NY and London, 17, 915-965.
11.
Lindahl, T., Karan, P., and Riazuddin, S. (1979). DNA G-glycosylases of E. coli. In: DNA repair mechanisms. Eds:
Hanawalt, P. and Friedberg, F.C., Academic Press, NY.
12.
Riazuddin, S. and Nasim, A. Future growth in biotechnology in the developing countries at the XVI International Congress
of Genetics at Toronto, Ontario, Canada, August 20-27, 1989. Genome, Vol. 31(2): 1042-1045.
13.
Grossman, L. and Riazuddin. S. (1979). Correndonucleases.
Friedberg, F.C., Academic Press, NY.
14.
Riazuddin, S. and Husnain, T. (1992). Transformation of chickpea (Cicer arietinum L.) In: Biotechnology in Agriculture and
Forestry, Ed. Y.P.S. Bajaj, Springer-Verlag, N.Y. USA.
15.
Riazuddin, S. (1994). Plant Genetic Engineering and Future Agriculture.
Methods, vol.16, p.93-113, Plenum Press, New York.
16.
Riazuddin, S. (2000). National Policy and Regulation. In: Biotechnology in the developing world and countries in
economic transition edited by G.T. Tzotzos and K.G. Skryabin, CABI Publishing, pages 142-146
17.
Ahmed, Z.M., Riazuddin, S., Friedman, T.B., Riazuddin, S., Wilcox, E.R. and Griffith, A.J. (2002). Clinical Manifestations
of DFNB29 Deafness. Adv. Otolaryyngol., 61: 156-160.
18.
Riazuddin, S., Ahmed, Z.M, Friedman, T.B., Griffith, A.J. Riazuddin, S. and Wilcox, E.R. (2002). Genetic Modifiers of
Hereditary Hearing Loss. Adv. Otolaryyngol., 61: 224–229.
18.
Rashid, B., Husnain, T., Riazuddin, S. (2010) Plant Adaptation and Phytoremediation, Chapter 19 Herbicides and
pesticides as potential pollutants – A global problem. Ashraf, M.; Ozturk, M.; Ahmad, M. S. A. (Eds.), pp 427-447,
Springer Netherlands-Dordrecht, Hardcover ISBN: 978-90-481-9369-1.http://dx.doi.org/10.1007/978-90-481-9370-7
In: DNA repair mechanisms. Eds: Hanawalt, P. and
In: Genetic Engineering Principles and
BOOKS
1.
Khan, A.M., Riazuddin, S., Qadir, A., Qazi, M.N. Physics and Contemporary Needs. Vol. 6. Plenum Press, New
York (1984).
2.
Shakoori, A.R., Hashmi, T.H., Riazuddin, S. and Shaikh, K.H., 1988. (Eds .) An Annotated Textbook of Biology for
Higher Secondary School , based on modernized curricula in Biology proposed for Secondary Schools in ISESCO
Member State , Islamic Educational, Scientific and Cultural Organization, Rabbat , Morocco , pages, 833
3.
Shakoori, A.R., Hashmi, T.H., Riazuddin, S. and Shaikh, K.H., 1988. (Eds .) An Annotated Textbook of Biology for
Secondary School , based on modernized curricula in Biology proposed for Secondary Schools in ISESCO
Member State , Islamic Educational, Scientific and Cultural Organization, Rabbat , Morocco , pages, 377.
Rashid, B., Husnain, T., Riazuddin, S. (2010). Gene Pyramiding: An Approach Towards Sustainable Insect
Resistance. by Publishers VDM Verlag Dr. Mϋllar Aktiengesellschaft & Co. Kg Dudweiler Landstr. 99,66123
Saarbrϋcken, Germany. ISBN 978-3-639-25629-1.
4.
51
LIST OF PATENTS

Patent #6150156 on “Bacillus thuringiensis isolates active against sucking
insects”

Patent No.137087 “Process for the Making of Bacillus thuringiensis
Biopesticides/ Bioinsecticides” through Patent Officer, Govt. of Pakistan,
Karachi.

Patent No.137121 on “Process Development for Fungal Biopesticide”
through Patent Officer, Govt. of Pakistan, Karachi.

Patent No. 138279 on “Methods for determination of Protein and DNA
continents for detection of Bacillus thuringiensis in Plant Products” through
Patent Officer, Govt. of Pakistan, Karachi.

Patent No.138287 on “Development of Basmati Rice containing multiple
transgenes” through Patent Officer, Govt. of Pakistan, Karachi.

Patent No. 138719 on “Assay system for molecular diagnosis (PCR-based
of Hepatitis C. Virus.

Patent No. PCT/US10/23437 “Diagnostic and Therapeutic uses of
GNPTAB, CNTPG and NAGPA in stuttering” (2010).

Patent No. 140587 “A method of purification of the recombinant protein
products. (2009)” through Pakistan patent office, Karachi.

Patent No. 140586 “A process for modification of recombinant human
interferon for therapeutic use. (2009) through Pakistan patent office,
Karachi.

Patent No. 140574 “An improved expression of codon optimized human
interferon” (2007) through Pakistan patent office, Karachi.
Patents submitted to USFDA

3-[(2-furyl-1-(3-hydroxyl-10, 13-dimethyl-tetra deca hydroxy-1H-cyclopenta
(ß) phenanthren-17-yl]-3-phenyl-2-propen-1-one or derivatives thereof for
treating or preventing antiviral infections

7-Nitro-2-(3-nitro phenyl)-4H-3,1-benzoxazin-4-one or derivatives thereof
for treating or preventing antiviral infections

3-amino-2-(4-nitrophenyl)-4-(3H)-quinazolinone or derivatives thereof for
treating or preventing antiviral infections
52
SUGGESTED REFEREES
Richard J. Roberts (Nobel Laureate), New England Biolabs., Inc., 240 County
Road, Ipswich, MA 01932-2723, USA.
[T.N.978-380-7405; Fax: 978-380-7406; Email: roberts@neb.com]
Thomas B. Friedman, Chief, Laboratory of Molecular Genetics, National Institute
on Deafness and Other Communication Disorders, National Institutes of Health, 5
Research court, Rockville, MD, USA.
[T.N. 301 496 7882; Fax: 301-402-7580 (private); Email: friedman@nidcd.nih.gov]
Lawrence A. Loeb, Ex-President, American Association for Cancer Research and
Director, Joseph Gottstein Memorial Cancer Res. Lab., K-Block, Health Sciences
Building, University of Washington, Seattle, WA98195, USA.
[T.N.206-435-6015; Fax: 206-543-3967; Email: lploeb@excite.com, phyllisloeb@earthlink.net]
Dennis Drayna, Chief, Section on Systems Biology of Communication Disorders
Laboratory of Molecular Genetics, National Institute of Deafness and
Communication Disorders,5 Research court, Rockville, MD, USA.
Email: drayna@nidcd.nih.gov
Amir Muhammed, Rector, National University of Computer & Emerging Sciences,
Founding Member BOG, CEMB, FAST House, Rohtas, Road, Sector G-9/4,
Islamabad, Pakistan.
[Tel: 92-51-2855072-4; Fax: 92-51-2855075; Email: amir@nu.edu.pk]
Muhammad Ashraf, PhD, Professor of Pathology, Pathology & Lab Med,
University of Cincinnati, MSB 1253A, P. O. Box 670529, Cincinnati OH 45267
[Tel: (513)558-0145; Email: muhammad.ashraf@uc.edu]
53