CHEM 2204 – Chemical Laboratory Techniques
Practical Lab Techniques
Preparation of Chemicals and Equipment
Technique #1 – Use of a Burette
-------------------------2 stations
Technique #2 – Use of a Pipette
-------------------------2 stations
Technique #3 – Use of a Volumetric Flask -----------------2 stations
Technique #4 – Weighing
----------------------------- balance room
Technique #5 – Use of a Bottle-Top Dispenser ------------2 stations
Technique #6 - Gravity Filtration -------------------------2 stations
- Vacuum Filtration ---------------------------2 stations
Technique #7 – Preparation of a Standard Solution ------2 stations
Technique #8 – Titration -------------------------------------4 stations
Total: -----------------------------------------------------------18 stations
January 4, 2011
Page 1
Technique #1 Station – Use of a Burette
– Set up two stations
Chemical: A reagent bottle of a slightly coloured distilled water (Use food
colouring to prepare the coloured water. Coloured water is easier to
see the air bubble.).
Glassware and equipment at each station:
1.
2.
3.
4.
5.
An Erlenmeyer flask
Beakers - 50 mL, 100 mL, 250 mL, 500 mL
Pasteur pipettes
Burette reader
Masking tape for labeling
January 4, 2011
Page 2
Technique #2 Station - Use of a Pipette
– Set up two stations
Chemical: A reagent bottle of distilled water (no additives).
Glassware and equipment at each station:
1. 4 volumetric pipettes: 5.00, 10.00, 25.00, and 50.00 mL on a pipette
rack.
2. Pipette bulbs: two size – we will use the simple pipette bulbs for this
course.
3. Pasteur pipettes
4. Masking tape for labeling
5. Beakers - 50 mL, 100 mL, 250 mL, 500 mL
January 4, 2011
Page 3
Technique #3 Station - Use of a Volumetric Flask
– Set up two stations
Chemical: A reagent bottle of a slightly coloured distilled water (Use food
colouring to prepare the coloured water.). Put a label on the reagent
bottle that says “Solution A - 2.010 M”
Draw Box: In the draw box, place these numbers:
Dilution Factor = 2
Dilution Factor = 5
Dilution Factor = 10
Dilution
Factor
Ci
(M)
Cf
(M)
Vi
(mL)
Vf
(mL)
2
2.010
1.005
50.00
100.0
5
2.010
0.4020
20.00
100.0
10
2.010
0.2010
10.00
100.0
Glassware and equipment at each station:
1. Beakers - 50 mL, 100 mL, 250 mL, 500 mL
2. 5 volumetric pipettes: 5.00, 10.00, 20.00, 25.00, and 50.00 mL on a
pipette rack
3. A 100.0 mL volumetric flask
4. Wash bottle
5. Small funnel
6. Masking tape for labelling
January 4, 2011
Page 4
Technique #4 Station – In the balance room
Chemical: Several vials of sand in the balance room. These can be the same sand
that is used for TE.
Glassware and equipment at each station:
1. Beakers - 50 mL, 100 mL, 250 mL, 500 mL
2. Weigh boats
January 4, 2011
Page 5
Technique #5 Station – Use of a Bottle-Top Dispenser
– Set up two stations
Chemical:
1. A reagent bottle of distilled water (no additives).
2. Small reagent bottle with a bottle-top dispenser – do not assemble
Draw Box: In the draw box, place these numbers:
Dispenser volume = 10.0 mL
Dispenser volume = 15.0 mL
Dispenser volume = 20.0 mL
Dispenser volume = 35.0 mL
Dispenser volume = 40.0 mL
Glassware and equipment at each station:
1. Beakers - 50 mL, 100 mL, 250 mL, 500 mL
2. A 50-mLgraduated cylinder
3. Masking tape for labeling
January 4, 2011
Page 6
Technique #6 Station – Gravity Filtration
– Set up two stations
Chemical:
1. A large beaker of sand and water mixture – Student will get a sample
of sand/water mixture from this stock mixture.
Glassware and equipment at each station:
1.
2.
3.
4.
5.
6.
7.
Long stem funnel
Funnel rack
Beakers - 50 mL, 100 mL, 250 mL, 500 mL
Rubber policeman
Wash bottle
Fast flow filter paper of different diameter (One of these should be
the correct size for the funnel provided.)
Masking tape for labeling
January 4, 2011
Page 7
Technique #7 Station – Preparation of a Standard Solution
– Set up two stations
Chemical:
1. Vials of CuSO4 5H2O in the balance room
Draw Box: In the draw box, place these numbers:
Standard solution concentration approximately
Standard solution concentration approximately
Standard solution concentration approximately
Standard solution concentration approximately
0.08 M
0.15 M
0.22 M
0.28 M
Mass of CuSO4 5H2O (g)
Concentraion (M)
0. 9980 g
0.0800 M
1.8713 g
0.1500 M
2.7445 g
0.2200 M
3.4930 g
0.2800 M
Molar Mass CuSO4 5H2O = 249.5 g/mole
Glassware and equipment at each station:
1.
2.
3.
4.
5.
January 4, 2011
A 50-mL volumetric flask
Beakers - 50 mL, 100 mL, 250 mL, 500 mL
A stir rod
A wash bottle
Masking tape for labeling
Page 8
Technique #8 Station – Titration
– Set up four stations
Chemical:
1. 0.01 M NaOH
2. 0.01 M HCl
3. Phenolpthalein
Glassware and equipment at each station:
1.
2.
3.
4.
5.
6.
7.
8.
January 4, 2011
Burette already acclimatized with the base. Label the burette NaOH/
A 125 mL Erlenmeyer flask
Beakers - 50 mL, 100 mL, 250 mL, 500 mL
A stir rod
A wash bottle
Masking tape for labeling
Burette reader
Pasteur pipettes
Page 9