Curriculum Vitae
Name: Kolot Mikhail
Birthdate: 17 April 1956
A. Academic Background
From-To
1980-1985
Institution
Moscow University, former USSR
1973-1978 Tashkent University, former USSR
B. Major research interest: Molecular Biology and Genetics
C. Employment (start with present position)
Biochemistry
From-To Institution
1999-present Tel-Aviv University
1991-1999 Tel-Aviv University
1990-1991 Institute of Molecular
Genetics, USSR Academy of Sciences
Area of specialization
Biochemistry
Research area
Molecular Biology/Biochemistry
Degree
PhD
M Sc
Title
Sr. Investigator
Molecular Biology/Biochemistry Res. Assoc.
Molecular Biology/Biochemistry Sr. Res. Asst.
1986-1990 Institute of Molecular
Genetics, USSR Academy of Sciences
Molecular Biology/Biochemistry Res. Asst.
1982-1986 Institute of Molecular
Genetics, USSR Academy
Molecular Biology/Biochemistry Jr. Res. Asst. of Sciences
D. List of publication (past five years only)
1 .Kolot, M.
and Yagil E. (2003) Determinants that target the integrase of phage HK022 into the mammalian nucleus. J. Molec. Biol. 325:629-635
2 .Kolot, M.
, Meroz A. and Yagil, E. (2003) Site-specific recombination in human cells catalyzed by the wild type integrase protein of coliphage HK022. Biotechnology &
Bioengineering 84:56-60.
3.Gottfried, P., Silberstein, N., Yagil, E and Kolot, M.
(2003) Activity of coliphage HK022 excisionase (Xis) in the absence of DNA binding. FEBS Letters 545:133-138.
4.Gottfried, P., Kolot, M., Silberstein, N. and Yagil E.
(2004) Protein-protein interaction between monomers of coliphage HK022 excisionase. FEBS Letters, 577:17-20
5.Gottfried, P., Lotan, O., Kolot, M., Maslenin, L., Bendov, R., Gorovits, R., Yesodi, V.,
Yagil, E. and Rosner, A.
(2005) Site-specific recombination in Arabidopsis plants promoted by the integrase protein of coliphage HK022. Plant Molecular Biology 57:435-
444.
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6.Harel-Levi, G., Goltsman J., Tuby C., Yagil, E. and M. Kolot . Site-specific recombination in the human genome catalyzed by coliphage HK022 integrase. Submitted for publication.
7.Kolot, M ., Gorovits, R. Silberstein, N., Fichman, B. and Yagil E. Phosphorylation of the
Integrase protein of coliphage HK022. Submitted for publication.
E Grants and awards (past three years)
Total grant
Publications
Research topic
Nuclear expression of a lambdoid site-specific recombination system in mammalian cells and its recruitment for site-specific gene insertions in eukariotes
Funding agency
Israel Science
Foundation
(in collaboration with
Prof. E. Yagil), 2002-
2006
150,000$
1, 2
Int phosphorylation, Xis dimerization and specificity
Recruitment of a phage site-specific recombination system for eukaryotic gene manipulations
Temporal gene inactivation in the brain of zebrafish by the use of a phage sitespecific recombinase
US-Israel Binational
Science Foundation
(BSF)
(in collaboration with
Prof. E. Yagil)
2004-2008
Israel Cancer Research
Fund (ICRF)
2004-2005
Adams foundation,
2007-2008
140,000$
60,000$
3,000$
3, 4, 7
1, 2
1, 2, 6
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