Principal Investigator/Program Director (Last, First, Middle):
Cowley, Jr., Allen Wilson
BIOGRAPHICAL SKETCH
Provide the following information for the key personnel and other significant contributors in the order listed on Form Page 2.
Follow this format for each person. DO NOT EXCEED FOUR PAGES.
NAME
POSITION TITLE
Cowley, Jr., Allen Wilson, Ph.D.
Professor and Chairman
eRA COMMONS USER NAME
acowley
EDUCATION/TRAINING (Begin with baccalaureate or other initial professional education, such as nursing, and include postdoctoral training.)
INSTITUTION AND LOCATION
Trinity College, Hartford, CT
Hahnemann Medical College, Philadelphia, PA
Hahnemann Medical College, Philadelphia, PA
University of Mississippi
DEGREE
(if applicable)
YEAR(s)
B.A.
M.S.
Ph.D.
Postdoctoral
1961
1965
1968
1968
FIELD OF STUDY
Economics
Physiology
Physiology
Physiology
A. Positions and Honors.
Positions and Employment
1969-1974
Assist. Professor of Physiology & Biophysics, Univ. of Mississippi Medical Center, Jackson, MS
1974
Assoc. Prof. of Physiology & Biophysics, Univ. of Mississippi Medical Center, Jackson, MS
1974-1975
Visiting Professor of Physiology, Harvard Medical School
1975-1980
Professor of Physiology & Biophysics, Univ. of Mississippi Medical Center, Jackson, MS
1976-1980
Director of Graduate Studies, Dept. of Physiology & Biophysics, Univ. of Mississippi Medical
Center, Jackson, MS
1980-present Professor and Chair, Dept. of Physiology, Medical College of Wisconsin, Milwaukee, WI
Honors
1973-78, Established Investigator, American Heart Association (AHA); 1979-83, NIH Study SectionCardiovascular and Renal; 1999-present, Editorial Board, Physiological Reviews; 1999-present, Sr. Editor,
Physiological Genomics; Associate Editor, American Journal of Physiology, Heart and Circulation; 1999-04;
Associate Editor, American Journal of Physiology: Regulatory, Comp., Integ., 2001-2005; Member, Board of
Directors, The Medical College of Wisconsin; 1988-93, Member, Council of the American Physiology Society
(APS); 1990-92, Chairman, AHA: Council for High Blood Pressure Research; 1994-95, NASA Life and
Microgravity Sciences and Applications Advisory Committee; 1993-97, Member, NHLBI Research Review
Committee A/B; 1993, Distinguished Service Award-Medical College of Wisconsin; 1996, Distinguished
Achievement Award - AHA; 1996, Starling Distinguished Lectureship of the APS; 1997, President of the APS;
1997, Wiggers Award Recipient, APS; 1997, Novartis Award Recipient, Council for High Blood Pressure
Research, AHA; 1998-2002, NHLBI Advisory Council; Doctor Honoris Causa, Semmelweis University of
Medicine, Budapest, Hungary; 2001-2005, President of the International Union of Physiological Sciences; 2002,
Walter B. Cannon Lectureship, APS; 2004, Distinguished Scientist Award, AHA; 2006, NHLBI Strategic Plan
Committee.
B. Selected peer-reviewed publications (in chronological order).
1. Cowley AW Jr, Stoll M, Greene AS, Kaldunski ML, Roman RJ, Tonellato PJ, Schork NJ, Jacob HJ.
Genetically defined risk of salt-sensitivity within a population of F2 rats from a Brown Norway and Dahl S
derived intercross. Physiol Genomics 2:107-115, 2000.
2. Johnson RJ, Gordon KL, Giachilli C, Kurth T, Skelton MM, Cowley AW Jr. Tubulointerstitial injury and loss
of nitric oxide synthases parallel the development of hypertension in the Dahl-SS rat. J Hypertension
18:1497-1505, 2000.
3. Stoll M, Cowley AW Jr, Tonellato PJ, Greene AS, Kaldunski ML, Roman RJ, Dumas P, Schork N, Wang Z,
Jacob HJ. A genomic-systems biology map for cardiovascular function. Science 294:1723-1726, 2001.
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
Page
9
Biographical Sketch Format Page
Principal Investigator/Program Director (Last, First, Middle):
Cowley, Jr., Allen Wilson
4. Liang M, Yuan B, Rute E, Greene AS, Zou A-P, Soares P, McQuestion G, Slocum GR, Jacob HJ, Cowley
AW Jr. Rat renal medullary genes associated with salt-sensitive hypertension identified by chromosomal
substitution and cDNA microarray. Phys Genomics 8:139-149, 2002.
5. Makino A, Zou A-P, Cowley AW Jr. Increased renal medullary oxidative stress produces hypertension.
Hypertension 39:667-672, 2002.
6. Mori T, Dickhout JG, Cowley AW Jr. Vasopressin stimulates nitric oxide release via Ca2+ signaling in inner
medullary collecting duct. Hypertension 39:465-469, 2002.
7. Dickhout JG, Mori T, Cowley AW Jr. Tubulovascular nitric oxide crosstalk: buffering of angiotensin IIinduced medullary vasoconstriction. Circ Res. 91:487-493, 2002.
8. Szentivanyi M Jr, Zou A-P, Mattson DL, Soares P, Moreno C, Roman RJ, Cowley AW Jr. Renal medullary
nitric oxide deficit of Dahl S rats enhances hypertensive actions of angiotensin II. Am J Physiol Regul Integr
and Comp Physiol. 283:R266-R272, 2002.
9. Mori T, Dickhout JG, Cowley AW Jr. Vasopressin increases intracellular NO concentration via Ca 2+
signaling in inner medullary collecting duct. Hypertension 39 [part 2]:465-469, 2002.
10. Cowley AW Jr. Genomics and homeostasis. Am J Physiol. 284:R611-R627, 2002.
11. Liang M, Yuan B, Rute E, Greene AS, Olivier M, Cowley AW Jr. Insights into Dahl salt-sensitive
hypertension revealed by temporal patterns of renal medullary gene expression. Physiol Genomics 12:229237, 2003.
12. Mori T, Cowley AW Jr. Angiotensin II-NAD(P)H oxidase-stimulated superoxide modifies tubulovascular
nitric oxide cross-talk in renal outer medulla. Hypertension 42(4):588-593, 2003.
13. Liang M, Briggs AG, Rute E, Greene AS, Cowley AW Jr. Quantitative assessment of the importance of dye
switching and biological replication in the cDNA miroarray studies. Physiol Genomics 14:199-207, 2003.
14. Makino A, Skelton MM, Zou A-P, Cowley AW Jr. Increased renal medullary H2O2 leads to hypertension.
Hypertension 42:25-30, 2003.
15. Cowley AW Jr, Mori T, Mattson DL, Zou A-P. Role of renal NO production in the regulation of medullary
blood flow. Am J Physiol 284:R1355-1369, 2003.
16. Yuan B, Liang M, Yang Z, Rute E, Taylor N, Olivier M, Cowley AW Jr. Gene expression reveals
vulnerability to oxidative stress and interstitial fibrosis of renal outer medulla to non-hypertensive elevations
of Ang II. Am J Physiol. 284(5):R1219-1230, 2003.
17. Moreno C, Dumas P, Kaldunski ML, Tonellato PJ, Greene AS, Roman RJ, Cheng Q, Wang Z, Jacob HJ,
Cowley AW Jr. Genomic map of cardiovascular phenotypes of hypertension in female Dahl S rats. Physiol
Genomics 15:243-257, 2003.
18. Mori T, Cowley AW Jr. Role of pressure in angiotensin II-induced renal injury: chronic servo-control of renal
perfusion pressure in rats. Hypertension 43:752-759, 2004.
19. Mori T, Cowley AW Jr. Renal oxidative stress in medullary thick ascending limbs produced by elevated
NaCl and glucose. Hypertension 43:341-346, 2004.
20. Cowley AW Jr, Roman RJ, Jacob HJ. Application of chromosomal substitution techniques in gene-function
discovery. J Physiol. 554:46-55, 2004.
21. Liang M, Cowley AW Jr, Greene AS. High throughput gene expression profiling: a molecular approach to
integrative physiology. J Physiol. 554:22-30, 2004.
22. Cowley AW Jr, Liang M, Roman RJ, Greene AS, Jacob HJ. Consomic rat model systems for physiological
genomics. Acta Physiol Scand. 181:585-592, 2004.
23. Liang M, Cowley AW Jr, Hessner MJ, Lazar J, Basile DP, Pietrusz JL. Transcriptome analysis and kidney
research: toward systems biology. Kidney Int. 67(6):2114-22, 2005.
24. Dwinell MR, Forster HV, Petersen J, Rider A, Kunert MP, Cowley AW Jr, Jacob HJ. Genetic determinants
on rat chromosome 6 modulate variation in the hypercapnic ventilatory response using consomic strains. J
Appl Physiol. 98(5):1630-8, 2005.
25. Mattson DL, Kunert MP, Roman RJ, Jacob HJ, Cowley AW Jr. Substitution of chromosome 1 ameliorates
L-NAME hypertension and renal disease in the fawn-hooded hypertensive rat. Am J Physiol Renal Physiol.
288(5):F1015-22, 2005.
26. Basile DP, Fredrich K, Alausa M, Vio CP, Liang M, Rieder MR, Greene AS, Cowley AW Jr. Identification of
persistently altered gene expression in the kidney after functional recovery from ischemic acute renal
failure. Am J Physiol Renal Physiol. 288(5):F953-63, 2005.
27. Taylor NE, Cowley AW Jr. Effect of renal medullary H2O2 on salt-induced hypertension and renal injury.
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
Page
10
Continuation Format Page
Principal Investigator/Program Director (Last, First, Middle):
Cowley, Jr., Allen Wilson
Am J Physiol Regul Integr Comp Physiol. 289(6):R1573-9, 2005.
28. Taylor NE, Glocka P, Liang M, Cowley AW Jr. NADPH oxidase in the renal medulla causes oxidative stress
and contributes to salt-sensitive hypertension in Dahl S rats. Hypertension. Epub 2/27, 2006.
29. Kwitek AE, Jacob HJ, Baker JE, Dwinell MR, Forster HV, Greene AS, Kunert MP, Lombard JH, Mattson
DL, Pritchard KA Jr., Roman RJ, Tonellato PJ, Cowley AW Jr. BN phenome: detailed characterization of
the cardiovascular, renal and pulmonary systems of the sequenced rat. Physiol Genomics 25:303-313,
2006.
30. Abe M, O’Connor P, Kaldunski M, Liang M, Roman RJ, Cowley AW Jr. Effect of sodium delivery on
superoxide and nitric oxide in the medullary thick ascending limb. Am J Physio. 291:F350-F357, 2006.
31. Taylor NE, Maier KG, Roman RJ, Cowley AW Jr. NO synthase uncoupling in the kidney of Dahl S rats:
Role of dihydrobiopterin. Hypertension 48:1066-1071, 2006.
32. Kunert MP, Drenjancevic-Peric I, Dwinell MR, Lombard JH, Cowley AW Jr, Greene AS, Kwitek AE, Jacob
HJ. Consomic strategies to localize genomic regions related to vascular reactivity in the Dahl salt-sensitive
rat. Physiol. Genomics 26:218-225, 2006.
33. Cowley AW Jr. The genetic dissection of essential hypertension. Nat Rev Genet. 7(11):829-840, 2006.
34. O’Connor PM, Cowley AW Jr. Vasopressin-induced nitric oxide production in rat inner medullary collecting
duct is dependent on V2 receptor activation of the phosphoinositide pathway. Am J Physiol Renal Physiol.
293(2):F526-32, 2007.
35. Mori T, O’Connor PM, Abe M, Cowley AW Jr. Enhanced superoxide production in renal outer medulla of
Dahl salt-sensitive rats reduces nitric oxide tubular-vascular cross-talk. Hypertension 49:1336-41, 2007.
36. Michalkiewicz M, Michalkiewicz T, Geurts AM, Roman RJ, Slocum GR, Singer O, Weihrauch D, Greene
AS, Kaldunski M, Verma IM, Jacob HJ, Cowley AW Jr. Efficient transgenic rat production by a lentiviral
vector. Am J Physiol Heart Circ Physiol. 293(1):H881-94, 2007.
C. Research Support
Ongoing Research Support
R01 HL081091-03 (Cowley)
5/1/06 – 4/30/11
NIH/NHLBI
Mechanisms of Hypertension-induced Renal Injury
The goal of this grant is to determine the role that pressure plays in provoking pathways of oxidative stress and
injury in the renal outer medulla during the development of Angiotensin II + L-NAME-induced hypertension in
Sprague Dawley rats.
Role: PI
U01 HL66579-08 (Jacob) PI
9/30/00 – 7/31/09
NIH/NHLBI
Knock-Out Rats for Physiological Genomics
The major goals of this project are to develop, phenotype, and distribute 56 consomic strains in the form of
reciprocal chromosomal substitutions.
Role: Co-Investigator/Component Director
P01 HL29587-27 (Cowley)
3/1/98 – 2/28/13
NIH/NHLBI
Blood Pressure – Determinants & Controllers
The studies in the Program Project Grant focus on the role of the kidney in the long-term control of arterial
pressure.
Role: PI
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
Page
11
Continuation Format Page
Principal Investigator/Program Director (Last, First, Middle):
Cowley, Jr., Allen Wilson
P01 HL082798-03 (Cowley)
8/1/06 – 6/30/11
NIH/NHLBI
Genetic & Physiological Basis of Salt-induced Hypertension
The goal of this grant is to determine the genetic and physiological basis of the protection from salt-sensitivity
of blood pressure that occurs with substitution of Brown Norway alleles into regions of the Dahl salt-sensitive
rat chromosome 13.
Role: PI
R37-HL036279-20 (Roman)
4/1/05 – 3/31/10
NIH/NHLBI
20-HETE-TGF-beta in Hypertension-Induced Renal Injury
The goal of this study is to determine the mechanisms of the impaired relaxation of cerebral and skeletal
muscle resistance arteries that occurs when normotensive animals are subjected to an elevated dietary salt
intake.
Role: Co-Investigator
Relevant Completed Support
R37 HL49219-13 (Cowley) PI
1/01/01 – 12/31/05
NIH/NHLBI
Renal V1 Vasopressin and Hypertension
To define the role of vasopressin in the normal regulation of renal medullary blood flow by studying the effect of
small physiological changes of vasopressin on pressure flow relationships in the kidney of the V1 and V2
receptors and localize these receptors in microdissected vessels using molecular techniques.
Role: PI
P50 HL54998-10 (Cowley) PI
2/01/96 – 6/30/06
NIH/NHLBI
SCOR-Molecular Genetics of Hypertension
To determine the genetic loci and specific genes whose expression results in hypertension and in important
phenotypic changes associated with hypertension.
Role: PI
PHS 398/2590 (Rev. 09/04, Reissued 4/2006)
Page
12
Continuation Format Page