University of Pennsylvania
DNA SEQUENCING FACILITY
Tel:
(215) 573-7407
Fax:
(215) 573-9327
E-mail: dnaseq@mail.med.upenn.edu
FTP: dnaseq.med.upenn.edu
Web: www.med.upenn.edu/genetics/dnaseq/
Mailing Address:
B1 Richards Building
3700 Hamilton Walk
Philadelphia, PA 19104
Request For Cloning and/or Sub-cloning
PI ____________________________
Fund No __________________________
Tel____________________
Date__________
Contact person (full name) ________________________
Tel ______________________
*E-mail (Required) ___________________________________ Fax ________________________
Type of DNA to be cloned and/or subcloned –
Donor vector (if applicable)
Clone Name
Vector
_____________
________
_____________
________
_____________
________
_____________
________
Recipient vector
Clone Name
(Final)
_____________
_____________
_____________
_____________
Insert size
________
________
________
________
Host strain
_________
_________
_________
_________
Total size
_______
_______
_______
_______
Vector
Host strain
Total size
Anibiotic
________
________
________
________
________
________
________
________
_________
_________
_________
_________
_______
_______
_______
_______
Anibiotic
__________
__________
__________
__________
Sequencing Primer
Name
Sequence
After completion do you want-
___ ___ ___ ___ ___ ___ ___ ___ ___
just the DNA ( )
or DNA transformed and prepped ( ), Scale of Preparation ____
Please attach a separate sheet with the maps of the donor vector (if applicable) and
the recipient vector. Mention if you have any preferred restriction site(s) to be used for
cloning.
Revised Sep 10, 2012