Quick Quotation Request: Custom Polyclonal Antibody Services
Instructions
1. Please complete this form, including the peptide sequence (if ordering peptide synthesis from GenScript) and email it
to antibody@genscript.com or fax to 1-732-210-0262
2. Our service representative will contact you with the quote
3. * Mandatory information
Customer Information
Name:
Institution:
Shipping address* (needed to determine shipping cost):
Phone:
Email:
Antibody Information
Is your antigen toxic or harmful to humans/animals?*
Yes
No
How many antibodies are you ordering?
What species should we use to produce your antibody?
Mouse
Rabbit
Do you know which GenScript custom antibody package you would like to order?
Goat
Chicken
Yes
Rat
No
If YES, what is the catalog number:
If NO, please answer the questions below.
Do you want GenScript to synthesize the peptides? *
Yes
No
If YES, please include the sequence information:
If NO, please describe your antigen:
Do you need Express Service (33-day protocol for RABBIT polyclonal package and 60-day protocol for monoclonal
antibodies)?
Yes
No
Do you need Western-GUARANTEED Peptide pAb Service (66-day protocol for RABBIT polyclonal package with affinity
purification)?
Yes
No
Do you need a phospho-specific antibody?
Yes
No
If YES, phosphorylation at which amino acid:
Do you want GenScript to purify the antibodies?*
If YES, which type would you like:
If YES, which method should we use:
rabbit, goat or chicken)
Pooled purification
Protein A
Yes
No
Separated purification (Not available for mouse/rat)
Protein G
Antigen Affinity (Available only for peptide/protein antigens,
Other:
AAALAC International Accredited
Are you ordering additional animals (standard protocols include 5 animals for mice and 2 animals for rabbits, goats and
chicken)?
Yes
No
If YES, how many:
Do you need a test bleed after 2nd immunization?
Yes
No
Do you want GenScript to keep your animal after the final boost?*
Yes
No
If Yes, for how many days?:
Do you need sterile filtration and package your antibody?
Yes
No
Do you need your antibody digested to produce Fab or F (ab')2 fragments?
Fab
If YES, please specify:
Do you need your antibody conjugated?
Biotin
No
F (ab')2
How many antibodies should be used for modification?
If YES, please specify:
Yes
Yes
HRP
% of purified antibody
No
FITC
Texas Red
How many antibodies should be used for modification?
Other:
% of purified antibody
Do you have previous experience raising antibodies against this antigen?
Yes
No
If YES, please include any related data and/or references about previous attempts.
Special instructions:
Project Information
Is this project for grant application purpose?
Yes
No
When will the project start?
Immediately
Within one month
Within 3 months
Half a year later
Quick Quotation Request: Custom Monoclonal Antibody Services
Instructions
1. Please complete this form, including the antigen sequence (if ordering peptide synthesis from
GenScript) and email it to antibody@genscript.com or fax to 1-732-210-0262
2. Our service representative will contact you with the quote
Customer Information
Name:
Institution:
Shipping address (needed to determine shipping cost):
Phone:
Email:
Antibody Information
Is your antigen toxic or harmful to humans/animals?
Yes
No
How many antibodies are you ordering?
Do you know which GenScript custom monoclonal antibody packages you would like to order?
No
AAALAC International Accredited
Yes
If YES, what are the catalog numbers:
If NO, please answer the questions below:
Describe your antigen:
Peptide
Protein
Cell-based
Do you want GenScript to design the peptides?
Yes
Other:
No
If YES, please paste the protein sequence or accession number here:
Do you want GenScript to synthesize the peptides?
Yes
No
If YES, and if you are designing the sequence yourself, please paste the peptide sequence here:
Do you need MonoExpress service? (60-day protocol)
Yes
What species should we use to develop your antibody?
No
Mouse
Rat
Do you want GenScript to produce additional amounts of your monoclonal antibody (standard amount is 5 ml of
culture supernatant)?
Yes
No
If YES, which method should we use:
In vivo production (ascites)
Roller bottle cell culture
If you are ordering in vivo production, how many mice should we use:
If you are ordering cell culture production, what culture volume do you need (in L):
Do you want GenScript to purify the antibodies?
If YES, which method(s) should we use:
Protein A
Yes
No
Protein G
Protein L
Ion Exchange
Do you need your antibody digested to produce Fab or F (ab')2 fragments?
If YES, please specify:
Fab
Do you need your antibody conjugated?
Biotin
Yes
No
Yes
No
Other:
F (ab')2
How many antibodies should be used for modification?
If YES, please specify:
Low Endotoxin
HRP
% of purified antibody
Yes
No
FITC
Other:
How many antibodies should be used for modification?
% of purified antibody
Are you ordering additional animals (standard protocols include 5 mice or 3 rats)?
If YES, how many:
Do you need a test bleed after 3rd immunization (2nd immunization for express service)?
Do you need test samples after fusion and initial ELISA screening?
Yes
Do you have previous experience raising antibodies against this antigen?
Yes
No
Yes
No
If YES, please include any related data and/or references about previous attempts.
Addition assay information
Do you need additional antigen screening?
Yes
No
If YES, please specify additional screening antigens information: (Name, buffer, concentration and storage, etc.)
Do you need additional screening services?
Yes
No
If YES, please complete following:
AAALAC International Accredited
No
Antisera after immunization:
Western-blot
Parental supernatants after fusion:
Subclonal supernatants after subcloning:
Purified antibodies after purification:
ICC/IHC
Western-blot
Western-blot
Western-blot
IP
ICC/IHC
FACS
IP
ICC/IHC
IP
ICC/IHC
other:
FACS
IP
FACS
FACS
other:
other:
other:
Additional testing material (all materials should be provided within 2 months after the project start):
Purified protein (20-30 μg is needed)
Cell line (2 vials frozen cells are needed, please fill in the cell line information sheet)
Cell lysate or Tissue lysate
slides (>2 slides per sample per test)
Other (such as plasmid or other materials prepared by GenScript):
Other information for testing material: (For example: buffer, concentration, inhibitors, storage and lysis method, etc.)
Available positive control antibody (For example: epitope tag antibodies)
Expected Molecular weight in Western blot:
Special instructions
Project Information
Is this project for grant application purpose?
Yes
No
When will the project start?
Immediately
Within one month
Within 3 months
AAALAC International Accredited
Half a year later