Quick Quotation Request: Custom Polyclonal Antibody Services
Customer Information
Name:
Institution:
Shipping address* (needed to determine shipping cost):
Phone:
Email:
Antibody Information
Is your antigen toxic or harmful to humans/animals?*
Yes
No
How many antibodies are you ordering?
What species should we use to produce your antibody?
Mouse
Rabbit
Goat
Chicken
Yes
No
Rat
Do you know which GenScript custom antibody package you would like to order?
If YES, what is the catalog number:
If NO, please answer the questions below.
Do you want GenScript to synthesize the peptides?*
Yes
No
If YES, please include the sequence information:
If NO, please describe your antigen:
Do you need Express Service (33-day protocol for RABBIT polyclonal package and 60-day protocol for monoclonal
antibodies)?
Yes
No
Do you need Western-GUARANTEED Peptide pAb Service (66-day protocol for RABBIT polyclonal package with
affinity purification)?
Yes
No
Do you need a phospho-specific antibody?
Yes
No
If YES, phosphorylation at which amino acid:
Do you want GenScript to purify the antibodies?*
If YES, which type would you like:
Yes
Pooled purification
If YES, which method should we use:
Separated purification (Not available for mouse/rat)
Protein A
peptide/protein antigens, rabbit, goat or chicken)
No
Protein G
Antigen Affinity (Available only for
Other:
Are you ordering additional animals (standard protocols include 5 animals for mice and 2 animals for rabbits, goats
and chicken)?
Yes
No
If YES, how many:
Do you need a test bleed after 2nd immunization?
Yes
No
AAALAC International Accredited
Do you want GenScript to keep your animal after the final boost?*
Yes
No
If Yes, for how many days?:
Do you need sterile filtration and package your antibody?
Yes
No
Do you need your antibody digested to produce Fab or F (ab')2 fragments?
If YES, please specify:
Fab
No
F (ab')2
How many antibodies should be used for modification?
Do you need your antibody conjugated?
If YES, please specify:
Yes
Biotin
Yes
% of purified antibody
No
HRP
FITC
Texas Red
How many antibodies should be used for modification?
Other:
% of purified antibody
Do you have previous experience raising antibodies against this antigen?
Yes
No
If YES, please include any related data and/or references about previous attempts.
Special instructions:
Project Information
Is this project for grant application purpose?
Yes
No
When will the project start?
Immediately
Within one month
Within 3 months
Half a
year later
Quick Quotation Request: Custom Monoclonal Antibody Services
Customer Information
Name:
Institution:
Shipping address (needed to determine shipping cost):
Phone:
Email:
Antibody Information
Is your antigen toxic or harmful to humans/animals?
Yes
No
How many antibodies are you ordering?
Do you know which GenScript custom monoclonal antibody packages you would like to order?
No
If YES, what are the catalog numbers:
If NO, please answer the questions below:
Describe your antigen:
Peptide
Protein
Do you want GenScript to design the peptides?
Cell-based
Yes
Other:
No
If YES, please paste the protein sequence or accession number here:
AAALAC International Accredited
Yes
Do you want GenScript to synthesize the peptides?
Yes
No
If YES, and if you are designing the sequence yourself, please paste the peptide sequence here:
Do you need MonoExpress service? (60-day protocol)
Yes
What species should we use to develop your antibody?
No
Mouse
Rat
Do you want GenScript to produce additional amounts of your monoclonal antibody (standard amount is 5 ml of
culture supernatant)?
Yes
No
If YES, which method should we use:
In vivo production (ascites)
Roller bottle cell culture
If you are ordering in vivo production, how many mice should we use:
If you are ordering cell culture production, what culture volume do you need (in L):
Do you want GenScript to purify the antibodies?
If YES, which method(s) should we use:
Yes
Protein A
No
Protein G
Protein L
Ion Exchange
Low Endotoxin
Other:
Do you need your antibody digested to produce Fab or F (ab')2 fragments?
If YES, please specify:
Fab
Yes
No
F (ab')2
How many antibodies should be used for modification?
Do you need your antibody conjugated?
Yes
If YES, please specify:
FITC
Biotin
HRP
% of purified antibody
No
Other:
How many antibodies should be used for modification?
% of purified antibody
Are you ordering additional animals (standard protocols include 5 mice or 3 rats)?
Yes
No
If YES, how many:
Do you need a test bleed after 3rd immunization (2nd immunization for express service)?
Do you need test samples after fusion and initial ELISA screening?
Yes
Do you have previous experience raising antibodies against this antigen?
Yes
No
No
Yes
No
If YES, please include any related data and/or references about previous attempts.
Addition assay information
Do you need additional antigen screening?
Yes
No
If YES, please specify additional screening antigens information: (Name, buffer, concentration and storage, etc.)
Do you need additional screening services?
Yes
No
If YES, please complete following:
Antisera after immunization:
Western-blot
Parental supernatants after fusion:
Western-blot
Subclonal supernatants after subcloning:
Purified antibodies after purification:
ICC/IHC
Western-blot
Western-blot
IP
ICC/IHC
ICC/IHC
ICC/IHC
FACS
IP
other:
FACS
IP
IP
other:
FACS
FACS
Additional testing material (all materials should be provided within 2 months after the project start):
Purified protein (20-30 μg is needed)
AAALAC International Accredited
other:
other:
Cell line (2 vials frozen cells are needed, please fill in the cell line information sheet)
Cell lysate or Tissue lysate
slides (>2 slides per sample per test)
Other (such as plasmid or other materials prepared by GenScript):
Other information for testing material: (For example: buffer, concentration, inhibitors, storage and lysis
method, etc.)
Available positive control antibody (For example: epitope tag antibodies)
Expected Molecular weight in Western blot:
Special instructions
Project Information
Is this project for grant application purpose?
Yes
No
When will the project start?
Immediately
Within one month
Within 3 months
year later
AAALAC International Accredited
Half a