OIE Collaborating Centre Reports
Activities in 2010
Title of Collaborating Centre:
Address of Collaborating Centre:
Surveillance and Control of Animal
Protozoan Diseases
National Research Center for Protozoan Diseases, Obihiro
University of Agriculture and Veterinary Medicine,
Obihiro, Hokkaido 080-8555, JAPAN
Tel.:
(+81-155) 49.56.47
Fax:
(+81-155) 49.56.43
e-mail address:
website:
igarcpmi@obihiro.ac.jp
http://www.obihiro.ac.jp/~protozoa
Name of Head of Centre
(Responsible Official):
Hiroshi Suzuki, Ph.D.
Name of OIE Contact Point:
Ikuo Igarashi, D.V.M., Ph.D.
Name of writer of this report
(if different from above)
Noboru Inoue, D.V.M., Ph.D.
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
1
Surveillance and Control of Animal Protozoan Diseases
Part I: Summary of activities specifically related to the mandate of
OIE Collaborating Centres
1.
Activities as a centre of research, expertise, standardisation and dissemination of techniques
within the remit of the mandate given by the OIE
As the National Research Center for Protozoan Diseases was designated as an OIE collaborating centre in May
2008, we had established one laboratory in 2008, and installed new laboratory equipments from 2008 to 2009
(Cell culture, Molecular cloning, PCR/LAMP, ELISA, IFAT, Immunochromatographic test can be carried out)
dedicated for the OIE collaborating centre-related activities. In 2010, in addition to the above mentioned OIE
laboratory, another new BSL2 level laboratory space allotted for serological tests and blood DNA extraction for
diagnostic tests and epidemiological surveillances, which prevents biological hazards caused by imported
biological samples.
2.
Proposal or development of any procedure that will facilitate harmonisation of international
regulations applicable to the surveillance and control of animal diseases, food safety or animal
welfare
Loop-mediated isothermal amplification (LAMP) for specific detection of Trypanosoma brucei, Babesia, and
Theileria has been developed. Evaluation and standardization are ongoing.
Research project was started in order to develop a immunochormatographic test (ICT) for Trypanosoma evansi
using recombinant tandem repeat antigens.
Development of LAMP kits for human African trypanosomosis (HAT) were carried out in collaboration with the
Foundation for Innovative New Diagnostics (FIND, Switzerland) and EIKEN CHEMICAL CO LTD.,
development of Trypanozoon specific LAMP kit was carried out. This will be completed by June-2011.
3.
Placement of expert consultants at the disposal of the OIE
Dr. Ikuo Igarashi (OIE RL expert for equine piroplasmosis and bovine babesiosis) and Dr. Noboru Inoue (OIE RL
expert for Surra) participated in the 2nd Global Conference of OIE Reference Laboratories and Collaborating
Centres, 21-23 June, 2010 at the OIE Headquarters, Paris.
Dr. Noboru Inoue (OIE RL expert for Surra) participated in the Ad hoc Group meeting on Diagnostic Tests for
Trypanosomoses, which was held from 20 June, 2010 at the OIE Headquarters, Paris.
Part II: Other activities related to the mandate
of OIE Collaborating Centres
4.
Provision of scientific and technical training, within the remit of the mandate given by the OIE, to
personnel from OIE Members
1. Advanced Research Course on International Animal Health was held at The National Research Center for
Protozoan Diseases, Obihiro, Japan, financed by Japan International Cooperation Agency (JICA). Duration of the
training course was 10 months and 5 young scientists from various countries (Iraq, Mongolia Thailand, and
Zambia) were trained for basic molecular biology and molecular diagnostics.
2. Seventeen graduate students and post-docs from developing countries (Afghanistan, Bolivia, China, Egypt,
Kenya, Korea, Mongolia, Philippines, Sri Lanka, Sudan, Thailand, Viet Nam) were trained under Animal Global
Health (AGH) program supported by the global COE program ("Frontier Program for Animal Global Health and
Hygiene") of our university for developing international human resource of sciences.
2
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
Surveillance and Control of Animal Protozoan Diseases
5.
Organisation of scientific meetings on behalf of the OIE
None
6.
Coordination of scientific and technical studies in collaboration with other laboratories,
organisations or collaborating centres
1. Collaboration, training and technology transfer to the National Research Institute for Equines, India is ongoing
under the OIE Twining Program (Equine Piroplasmosis, Surra and Dourine).
2. Molecular epidemiological survey of Theileria orientalis in Viet Nam was carried out in collaboration with Hue
University (Prof. Lan, T. B.).
3. Financed by JSPS: Study of the animal trypanosomoses in Northern Province, Zambia. Molecular
epidemiological survey of human and animal African trypanosomoses was carried out. Counterpart: Dr. Boniface
Namangala, University of Zambia, and Prof. Chihiro Sugimoto, Hokkaido University. Total blood DNA from 391
cattle, 36 goats and 546 tsetse flies were subjected to PCR or LAMP tests for Trypanozoon, T. b. rhodesiense, T.
congolense and T. vivax.
4. Epidemiological studies on animal babesiosis and theileriosis in China were carried out in collaboration with
Shanghai Veterinary Research Institute (Dr. Zhou, J., Dr. Zhang, H.), Yanbian University (Dr. Zhang, S.), Fujian
Normal University (Dr. Huang, X.).
5. Epidemiological studies on animal babesiosis and theileriosis in Monglia were carried out in collaboration with
Institute of Veterinary Medicine (Dr. Battsetseg, B.)
6. Epidemiological studies on animal babesiosis and theileriosis in Thailan were carried out in collaboration with
Kasetsart University (Dr. Inpankaew, T., Dr. Jittapalapong, S.)
7. Evaluation of selorogical tests for equine piroplasmosis is carrying out in collaboration with Centre for
Foodborne & Animal Parasitology (OIE Reference Laboratory & Collaborating Centre), Canadian Food
Inspection Agency (Dr. Alvin A. Gajadhar).
8. Research project was started in order to develop a immunochormatographic test (ICT) and LAMP for Babesia
microti in collaboration with Yale School of Medicine (Dr. P. Krause).
9. Research project on molecular epidemiology in bovine babesiosis was done in collaboration with Kasetsart
University (Dr. S. Jittapalapong).
10. Research project on molecular epidemiology in bovine babesiosis was done in collaboration with Shanghai
Veterinary Institute (Dr. Prof. J. Zhou).
7.
Publication and dissemination of any information within the remit of the mandate given by the
OIE that may be useful to Members of the OIE
Scientific publications in peer-reviewed journals (last 1year)
1: Shinzawa N, Kanuka H. 2010, Host tolerance and infectious disease. Seikagaku, 82(11):1051-5. In Japanese.
PubMed PMID: 21226336.
2: Khukhuu A, Lan DT, Long PT, Ueno A, Li Y, Luo Y, Macedo AC, Matsumoto K, Inokuma H, Kawazu SI,
Igarashi I, Xuan X, Yokoyama N. 2010, Molecular Epidemiological Survey of Theileria orientalis in Thua Thien
Hue Province, Vietnam. J Vet Med Sci., PubMed PMID: 21187678. In press.
3: Nishikawa Y, Ibrahim HM, Kameyama K, Shiga I, Hiasa J, Xuan X. 2010, Host Cholesterol Synthesis
Contributes to Growth of Intracellular Toxoplasma gondii in Macrophages. J Vet Med Sci., PubMed PMID:
21187676. In Press.
Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
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Surveillance and Control of Animal Protozoan Diseases
4: Terkawi MA, Huyen NX, Wibowo PE, Seuseu FJ, Aboulaila M, Ueno A, Goo YK, Yokoyama N, Xuan X,
Igarashi I. 2010, Spherical body protein 4 is a new serological antigen for the global detection of Babesia bovis
infection in cattle. Clin Vaccine Immunol., PubMed PMID: 21123520. In Press.
5: Luo Y, Jia H, Terkawi MA, Goo YK, Ooka H, Li Y, Yu L, Cao S, Yamagishi J, Nishikawa Y, Saito-Ito A,
Igarashi I, Xuan X. 2010, Identification and characterization of a novel secreted antigen 1 of Babesia microti and
evaluation of its potential use in enzyme-linked immunosorbent assay and immunochromatographic test. Parasitol
Int., PubMed PMID: 21070864. In Press.
6: Yokoyama N, Ueno A, Mizuno D, Kuboki N, Khukhuu A, Igarashi I, Miyahara T, Shiraishi T, Kudo R, Oshiro
M, Zakimi S, Sugimoto C, Matsumoto K, Inokuma H. 2010, Genotypic Diversity of Theileria orientalis Detected
from Cattle Grazing in Kumamoto and Okinawa Prefectures of Japan. J Vet Med Sci., PubMed PMID: 20978366.
In Press.
7: Zhang G, Huang X, Boldbaatar D, Battur B, Battsetseg B, Zhang H, Yu L, Li Y, Luo Y, Cao S, Goo YK,
Yamagishi J, Zhou J, Zhang S, Suzuki H, Igarashi I, Mikami T, Nishikawa Y, Xuan X. 2010, Construction of
Neospora caninum stably expressing TgSAG1 and evaluation of its protective effects against Toxoplasma gondii
infection in mice. Vaccine. 28(45):7243-7. PubMed PMID: 20832493.
8: Tumurjav B, Terkawi MA, Zhang H, Zhang G, Jia H, Goo YK, Yamagishi J, Nishikawa Y, Igarashi I, Sugimoto
C, Xuan X. 2010, Serodiagnosis of ovine toxoplasmosis in Mongolia by an enzyme-linked immunosorbent assay
with recombinant Toxoplasma gondii matrix antigen 1. Jpn J Vet Res. 58(2):111-9. PubMed PMID: 20715421.
9: Ibrahim HM, Xuan X, Nishikawa Y. 2010, Toxoplasma gondii cyclophilin 18 regulates the proliferation and
migration of murine macrophages and spleen cells. Clin Vaccine Immunol. 17(9):1322-9. PubMed PMID:
20660134.
10: Ooka H, Terkawi MA, Goo YK, Luo Y, Li Y, Yamagishi J, Nishikawa Y, Igarashi I, Xuan X. 2011, Babesia
microti: Molecular and antigenic characterizations of a novel 94-kDa protein (BmP94). Exp Parasitol. 127(1):28793. PubMed PMID: 20599995.
11: Goo YK, Terkawi MA, Jia H, Aboge GO, Ooka H, Nelson B, Kim S, Sunaga F, Namikawa K, Igarashi I,
Nishikawa Y, Xuan X. 2010, Artesunate, a potential drug for treatment of Babesia infection. Parasitol Int.
59(3):481-6. PubMed PMID: 20541037.
12: Doi Y, Shinzawa N, Fukumoto S, Okano H, Kanuka H. 2010, ADF2 is required for transformation of the
ookinete and sporozoite in malaria parasite development. Biochem Biophys Res Commun. 397(4):668-72.
PubMed PMID: 20529666.
13: AbouLaila M, Yokoyama N, Igarashi I. 2010, Development and evaluation of two nested PCR assays for the
detection of Babesia bovis from cattle blood. Vet Parasitol. 172(1-2):65-70. PubMed PMID: 20471176.
14: Nishikawa Y, Zhang H, Ibrahim HM, Yamada K, Nagasawa H, Xuan X. 2010, Roles of CD122+ cells in
resistance against Neospora caninum infection in a murine model. J Vet Med Sci. 72(10):1275-82. PubMed
PMID: 20460838.
15: Iseki H, Kawai S, Takahashi N, Hirai M, Tanabe K, Yokoyama N, Igarashi I. 2010, Evaluation of a loopmediated isothermal amplification method as a tool for diagnosis of infection by the zoonotic simian malaria
parasite Plasmodium knowlesi. J Clin Microbiol. 48(7):2509-14. PubMed PMID: 20444968.
16: Thekisoe OM, Rodriguez CV, Rivas F, Coronel-Servian AM, Fukumoto S, Sugimoto C, Kawazu S, Inoue N.
2010, Detection of Trypanosoma cruzi and T. rangeli infections from Rhodnius pallescens bugs by loop-mediated
isothermal amplification (LAMP). Am J Trop Med Hyg. 82(5):855-60. PubMed PMID: 20439966.
17: Ueno A, Dautu G, Saiki E, Haga K, Igarashi M. 2010, Toxoplasma gondii deoxyribose phosphate aldolase-like
protein (TgDPA) interacts with actin depolymerising factor (TgADF) to enhance the actin filament dynamics in
the bradyzoite stage. Mol Biochem Parasitol. 173(1):39-42. PubMed PMID: 20433874.
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
Surveillance and Control of Animal Protozoan Diseases
18: Herbas MS, Ueta YY, Ichikawa C, Chiba M, Ishibashi K, Shichiri M, Fukumoto S, Yokoyama N, Takeya M,
Xuan X, Arai H, Suzuki H. 2010, Alpha-tocopherol transfer protein disruption confers resistance to malarial
infection in mice. Malar J. 9:101. PubMed PMID: 20403155.
19: Iseki H, Zhou L, Kim C, Inpankaew T, Sununta C, Yokoyama N, Xuan X, Jittapalapong S, Igarashi I. 2010,
Seroprevalence of Babesia infections of dairy cows in northern Thailand. Vet Parasitol. 170(3-4):193-6. PubMed
PMID: 20363078.
20: Kawazu S, Takemae H, Komaki-Yasuda K, Kano S. 2010, Target proteins of the cytosolic thioredoxin in
Plasmodium falciparum. Parasitol Int. 59(2):298-302. PubMed PMID: 20307687.
21: AbouLaila M, Sivakumar T, Yokoyama N, Igarashi I. 2010, Inhibitory effect of terpene nerolidol on the
growth of Babesia parasites. Parasitol Int. 59(2):278-82. PubMed PMID: 20178862.
22: Goto Y, Carter D, Guderian J, Inoue N, Kawazu S, Reed SG. 2010, Upregulated expression of B-cell antigen
family tandem repeat proteins by Leishmania amastigotes. Infect Immun. 78(5):2138-45. PubMed PMID:
20160013.
23: Kawase O, Nishikawa Y, Bannai H, Igarashi M, Matsuo T, Xuan X. 2010, Characterization of a novel
thrombospondin-related protein in Toxoplasma gondii. Parasitol Int. 59(2):211-6. PubMed PMID: 20144733.
24: Zhang H, Nishikawa Y, Yamagishi J, Zhou J, Ikehara Y, Kojima N, Yokoyama N, Xuan X. 2010, Neospora
caninum: application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for
reduction of offspring mortality from infection in BALB/c mice. Exp Parasitol. 125(2):130-6. PubMed PMID:
20097198.
25: Aonuma H, Yoshimura A, Kobayashi T, Okado K, Badolo A, Nelson B, Kanuka H, Fukumoto S. 2010, A
single fluorescence-based LAMP reaction for identifying multiple parasites in mosquitoes. Exp Parasitol.
125(2):179-83. PubMed PMID: 20064511.
26: AbouLaila M, Yokoyama N, Igarashi I. 2010, Development and evaluation of a nested PCR based on spherical
body protein 2 gene for the diagnosis of Babesia bovis infection. Vet Parasitol. 169(1-2):45-50. PubMed PMID:
20056327.
27: Aboulaila M, Yokoyama N, Igarashi I. 2010, Inhibitory effects of (-)-epigallocatechin-3-gallate from green tea
on the growth of Babesia parasites. Parasitology. 137(5):785-91. PubMed PMID: 20025823.
28: Jia H, Nishikawa Y, Luo Y, Yamagishi J, Sugimoto C, Xuan X. 2010, Characterization of a leucine
aminopeptidase from Toxoplasma gondii. Mol Biochem Parasitol. 170(1):1-6. PubMed PMID: 19931316.
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Annual reports of OIE Reference Laboratories and Collaborating Centres, 2010
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