2211 Seminole Dr. Huntsville AL, 35805 (800) 688-2248 www.operon.com Next-Generation Sequencing Services Customer Name Vince Buonaccorsi Institution : Juniata College Address Line 1 Address Line 2 Phone : 814-641-3579 E-mail : buonaccorsi@juniata.edu PRICE QUOTATION for NGS SERVICES Date : Feb 14, 2012 Quotation No : SE-IC-02142012-01 Valid Until : Mar 31, 2012 Sales Agent : Iris Cheung Phone : 617-447-1014 Email : IrisCheung@eurofins.com Library Constructions Services Shotgun DNA, ChIP-Seq or RNA library Prokaryotic RNA library Unit Price $276 $355 Illumina Sequencing Run Services HiSeq2000 2X100 bases (Paired-End) HiSeq2000 2X50 bases (Paired-End) Lane Price $2,850 $1,950 Bioinformatics Analysis Re-sequencing or Transcriptome Analysis Lane Price Starting from $500 Service Terms and conditions: Only Biosafety level 1 samples are accepted. Turnaround time for delivery of results is 4 to 8 weeks unless otherwise noted. FastQ result files as well as analysis reports, if applicable, will be shipping on a hard drive and/or made available through a web interface. We charge $150 for data delivery for all projects. Data generated will be held confidential and Eurofins MWG|Operon will claim no right whatsoever to all or any of the data. Eurofins MWG|Operon will store FastQ files for three months after the completion of the project. Web account for bioinformatic analysis will be available for one month. 2211 Seminole Dr. Huntsville AL, 35805 (800) 688-2248 www.operon.com Next-Generation Sequencing Services Service Descriptions and Sample Submission Guidelines Service Description Library Construction Illumina Sequencing Bioinformatics Exome Capture Input QC: BioAnalyzer®, Qbit® and/or Nanodrop® measurements Library Creation (may include): fragmentation, cDNA generation, adaptor ligation, size selection and PCR amplification Output QC: BioAnalyzer®, Qbit® and Q-PCR (optional) Cluster generation on the cBot automated station. Flowcell run on the HiSeq® 2000 per run module selected. Personalized secure online account where secondary and tertiary genome or transcriptome analysis can be generated and download. Includes 90 days access to the online account and personalized bioinformatics support. Capture of target regions using SureSelect® technology from Agilent. Clustering and running of libraries on HiSeq® 2000 using 100 Paired-End reads. Alignment of data, primary and secondary analysis (optional) with results accessible through personalized secure online account. Minimum Sample Requirements for Library Construction Shotgun DNA 5 µg at 20-100 ng/µL; 260/280 of 1.7 or above; 260/230 of 1.7 or above ChIP-Seq 50 ng purified ChIP-enriched DNA at 5-10 ng/µL; gel image of processed ChIP Standard RNA 5 µg at 0.5-1 ug/µL; RIN of 8 or above; 260/280 of 1.8; 260/230 of 1.7 FFPE RNA 500 ng at 50-60 ng/µL; 260/280 ration 1.8; no RIN necessary Low Input RNA 5 ng at 500 pg/µl; 260/280 of 1.8; RIN of 8 preferable. (up to 100 ng at higher concentration preferable) Prokaryotic RNA 20 ug at 1µg/µL or higher; RIN of 8; 260/280 of 1.8. We do not accept blood or tissue, please submit purified genomic DNA or total RNA in water or TE buffer. Ship overnight in dry ice. Sample Requirements for Clustering and Sequencing of Already made Libraries 1. Normalized Libraries or Library Pools at 10 nM concentration. 2. BioAnalyzer Trace and gel picture if available. 3. List of all MID/barcodes to multiplex samples. 2211 Seminole Dr. Huntsville AL, 35805 (800) 688-2248 www.operon.com Next-Generation Sequencing Services SAMPLE SUBMITION SHEET Please fill out information about your samples and include it with your sample shipment to Eurofins MWG|Operon. Also include a copy of the Purchase Order or other payment information. Quotation #:_____________________ Material: Name/ID Total RNA Genomic DNA Organism / Strain / Source *for Total RNA samples only Vol. (µl) Enrichment/Amplicons Conc. (µg/µl) 280/260 RIN * Buffer Please attach additional sheet if sending more then 10 samples We highly recommend concentration measurement by fluorescence (such as PicoGreen®) and quality of the sample be checked by Bioanalyzer® or gel electrophoresis. There will be a $50 charge per additional sample to be QCed if the initial material does not provide minimum quality/quantity for library construction. We only accept biosafety level 1 material. RNA isolated with phenol, TRIzol® or similar chemical reagents? Yes Other Notes/Comments about the samples or project: Please ship samples overnight in dry ice to the following address: Eurofins MWG|Operon NGS Sequencing Department 2211 Seminole Dr. Huntsville, AL 35805 USA No