2211 Seminole Dr.
Huntsville AL, 35805
(800) 688-2248
www.operon.com
Next-Generation Sequencing Services
Customer Name Vince Buonaccorsi
Institution : Juniata College
Address :
Phone : 814-641-3579
E-mail : buonaccorsi@juniata.edu
PRICE QUOTATION for NGS SERVICES
Date : May 15, 2012
Quotation No : SE-IC-05152012-03
Valid Until : June 30, 2012
Sales Agent : Iris Cheung
Phone : 617-447-1014
Email : IrisCheung@eurofins.com
Library Constructions Services
Shotgun DNA, ChIP-Seq or RNA library
Prokaryotic RNA library
Unit Price
$276
$355
Illumina Sequencing Run Services
HiSeq2000 2X100 bases (Paired-End)
HiSeq2000 2X50 bases (Paired-End)
HiSeq2000 1X50 bases (Single-Read)
HiSeq2000 1X100 bases (Single-Read)
Lane Price
$3,100
$2,100
$1,500
$2,400
Bioinformatics Analysis
Re-sequencing or Transcriptome Analysis
Lane Price
Starting from $500
Service Terms and conditions:
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Only Biosafety level 1 samples are accepted.
Turnaround time for delivery of results is 4 to 8 weeks unless otherwise noted.
FastQ result files as well as analysis reports, if applicable, will be shipping on a hard drive and/or
made available through a web interface. We charge $150 for data delivery for all projects.
Data generated will be held confidential and Eurofins MWG|Operon will claim no right whatsoever to
all or any of the data.
Eurofins MWG|Operon will store FastQ files for three months after the completion of the project. Web
account for bioinformatic analysis will be available for one month.
2211 Seminole Dr.
Huntsville AL, 35805
(800) 688-2248
www.operon.com
Next-Generation Sequencing Services
Service Descriptions and Sample Submission Guidelines
Service Description
Library Construction
Illumina Sequencing
Bioinformatics
Exome Capture
Input QC: BioAnalyzer®, Qbit® and/or Nanodrop® measurements
Library Creation (may include): fragmentation, cDNA generation, adaptor
ligation, size selection and PCR amplification
Output QC: BioAnalyzer®, Qbit® and Q-PCR (optional)
Cluster generation on the cBot automated station. Flowcell run on the
HiSeq® 2000 per run module selected.
Personalized secure online account where secondary and tertiary
genome or transcriptome analysis can be generated and download.
Includes 90 days access to the online account and personalized
bioinformatics support.
Capture of target regions using SureSelect® technology from Agilent.
Clustering and running of libraries on HiSeq® 2000 using 100 Paired-End
reads. Alignment of data, primary and secondary analysis (optional) with
results accessible through personalized secure online account.
Minimum Sample Requirements for Library Construction
Shotgun DNA
5 µg at 20-100 ng/µL; 260/280 of 1.7 or above; 260/230 of 1.7 or above
ChIP-Seq
50 ng purified ChIP-enriched DNA at 5-10 ng/µL; gel image of processed ChIP
Standard RNA
5 µg at 0.5-1 ug/µL; RIN of 8 or above; 260/280 of 1.8; 260/230 of 1.7
FFPE RNA
500 ng at 50-60 ng/µL; 260/280 ration 1.8; no RIN necessary
Low Input RNA
5 ng at 500 pg/µl; 260/280 of 1.8; RIN of 8 preferable.
(up to 100 ng at higher concentration preferable)
Prokaryotic RNA
20 ug at 1µg/µL or higher; RIN of 8; 260/280 of 1.8.
We do not accept blood or tissue, please submit purified genomic DNA or total RNA in
water or TE buffer. Ship overnight in dry ice.
Sample Requirements for Clustering and Sequencing of Already made Libraries
1. Normalized Libraries or Library Pools at 10 nM concentration.
2. BioAnalyzer Trace and gel picture if available.
3. List of all MID/barcodes to multiplex samples.
2211 Seminole Dr.
Huntsville AL, 35805
(800) 688-2248
www.operon.com
Next-Generation Sequencing Services
SAMPLE SUBMITION SHEET
Please fill out information about your samples and include it with your sample shipment
to Eurofins MWG|Operon. Also include a copy of the Purchase Order or other payment
information.
Quotation #:_____________________
Material:
Name/ID
Total RNA
Genomic DNA
Organism / Strain / Source
*for Total RNA samples only
Vol. (µl)
Enrichment/Amplicons
Conc. (µg/µl)
280/260
RIN *
Buffer
Please attach additional sheet if sending more then 10 samples
We highly recommend concentration measurement by fluorescence (such as PicoGreen®) and quality of
the sample be checked by Bioanalyzer® or gel electrophoresis. There will be a $50 charge per additional
sample to be QCed if the initial material does not provide minimum quality/quantity for library construction.
We only accept biosafety level 1 material.
RNA isolated with phenol, TRIzol® or similar chemical reagents?
Yes
Other Notes/Comments about the samples or project:
Please ship samples overnight in dry ice to the following address:
Eurofins MWG|Operon
NGS Sequencing Department
2211 Seminole Dr.
Huntsville, AL 35805
USA
No