Unidade Serviços Analíticos
Av. República, Qta do Marquês
2784-505 Oeiras, Portugal
Tel. 351 – 214469637; Fax 351 – 214421161;
mregalla@itqb.unl.pt
(to be filled by the laboratory)
PROTEIN SEQUENCING REQUEST FORM
RF LA ______________
A ___________________
Order date ______/____/____
Rubric ____________
Customer ID
Name:
Institution:
Address:
Department / Group:
VAT nº / Project:
Phone:
Fax:
e-mail:
Sample/s ID and characterization (fill in what is known)
I
II
III
IV
Sample name
Biological source
Estimated molecular mass (Da)
Estimated amount (μg/pmol)
Membrane bound (Y/N)
Monomeric (Y/N)
Samples in blott : Stain (X)
Samples in liquid or dried
Ponceau S
Buff er composition
Coomassie R
Buffer concentration
Coomassie G
Detergent name
and concentration
Amido Black
Aim of analysis
1 - N-terminal sequencing
2 - Internal sequencing / peptide mapping
Alkylation before sequencing
Enzyme(s) /Chemical to be used
RP-HPLC purification of peptides
Number of residues requested
N. of peptides to be sequenced
29LDr03 Anexo 1 Pág.1(2)
V
VI
Unidade Serviços Analíticos
Av. República, Qta do Marquês
2784-505 Oeiras, Portugal
Tel. 351 – 214469637; Fax 351 – 214421161;
mregalla@itqb.unl.pt
Storage (X)
Completion (X)
Room temperature
Dispose of sample
Refrigerated
Save for requester
Frozen
Light sensitive
Any other remarks:
Important Notes:
1. Warnings
Radioactive or biohazard samples are not accepted.
Only PVDF membrane is compatible with sequencing reagents.
Samples with improperly filled ordering form will not be processed.
If not requested, the remaining of the samples will be disposed of six months after analysis
2. Data measured by the Analytical Services Unit
Publications that use data measured by the Analytical Services Unit, should include this
information in the appropriate section. A typical sentence: “Data provided/obtained by the
Analytical Laboratory, Analytical Services Unit, Instituto de Tecnologia Química e Biológica,
Universidade Nova de Lisboa”.
Customer signature
_________________________________
29LDr03 Anexo 1 Pág.2(2)