Animal Hazardous Agents Form – Part II
It is the Principal Investigator’s responsibility to post the final version of
this form on the lab/animal room door two weeks prior to start of protocol.
Department:
Protocol #:
Room:
Building:
Campus
Phone
Authorized Personnel
Date last revised:
Date Posted:
Home Phone
Pager/Mobile
Principal Investigator:
Authorized Personnel:
Authorized Personnel:
Precautions for this protocol (for assistance, contact OEH&S and/or DLAR)
Animal Species:
Type of Hazard
Biohazardous Agents**:
Check category and list agent(s)
[ ] BSL 1:
[ ] BSL 2:
[ ] human cell lines or human tumors:
Location of Biosafety Cabinet
Chemical Agents (including gas
anesthetics)**:
Indicate which toxin is being used here:
Radioisotopes**:
How long should precautions be
followed?
(Note: You must notify DLAR each time
these precautions should be instituted)
[x] 1 week (treat bedding as hazardous for 7 days)
[ ] Duration of project
[ ] Other:
Protective Clothing:
[x] gloves
[x] dust mask
[ ] N95 respirator
[ ] foot covers
[ ] head cover/ bonnet
[ ] safety
glasses
[ ] goggles [x] sleeves (while working in biological safety cabinet – for
personal protection and maintains sterile conditions)
[x] for lab/ research staff: gown or lab coat
[ ] other:
Route of Animal Excretion:
[ ] urine
[ ] feces
[ ] respiratory
[x] none expected
[ ] other:____________________
Animal Carcass Disposal:
[x] routine incineration
[x] chemical waste
[ ] biohazardous waste [ ] radioactive waste
[x] other: carcasses stored in biohazard bag prior to incineration ^
Biohazardous Caging/Bedding Disposal:
[ ] regular waste
[ ] autoclave then treat as regular waste
[ ] saliva
Chemical or Radioactive Caging/Bedding
Disposal:
Bedding:
[ ] regular waste
[x] chemotherapeutic/chemical waste
[ ] radioactive decay
[ ] radioactive waste
Caging:
[x] regular waste
[ ] autoclave then treat as regular waste
[ ] radioactive decay
[ ] other:
Surface and Equipment Decontamination
[ ] autoclave
[x] bleach
[x] Spor-Klenz
[x] quat. ammonia compound
[ ] regular cage wash procedure
[
[x] any of the above marked options
Note: avoid using bleach on stainless steel surfaces (biosafety cabinets)
Animal Handling Precautions:
[ ] room pressurization negative to corridor
[x] Class II biological safety cabinet used for cage changes and animal
handling (req. for BSL 2). Chemical fume hoods may also be acceptable®.
Bedding must be collected as a hazardous waste for the first 7 days after
toxin injection. Contaminated bedding must be emptied from cages using a
Class I dump station, a Class II biosafety cabinet or a chemical fume hood®.
Waste is placed in a yellow chemical hazards bag for disposal by OEHS.
Additional Precautions: (e.g.:
immunizations, restricted access, special
decontamination, specific waste disposal,
etc.)
After this first cage change there is no need for further special precautions
to be taken regarding the animals or the cages as long as the animals have
not received additional toxin. Routine cage change procedures can be
followed after the first week.
Avoid using bleach (any strength) on stainless steel surfaces. Bleach will
begin to corrode stainless steel surfaces or equipment.
Toxin will not be deactivated by steam heat.
®Check with the PI first to make sure if sterile conditions must be maintained before using a chemical fume hood for cage
changes.
**NOTE: Individual cages must be identified with the hazardous agent, date of administration and appropriate hazard label
(chemical hazard sticker).
^Bags containing carcasses/ tissue must be labeled or tagged with the PI name and IACUC protocol number.
1. My staff and I will comply with all standards for animal care and investigation established in the Guide for the Care and Use of
Laboratory Animals and the Federal Animal Welfare Act, and will follow all policies established by the University to assure that these
standards are met.
2. I assume responsibility for the work described here.
3. All individuals working with the animals on this protocol are qualified by virtue of training or experience to perform proper handling,
experimental, and restraint techniques required for the species to be used.
4. I recognize my responsibility to identify occupational health hazards related to this protocol including identifying hazards, providing
the necessary training for those involved, and supplying the appropriate protective clothing and equipment to minimize the risks.
5. This research does not represent unnecessary duplication of previous experiments.
6. I realize that failure to adhere to policies related to animal care and use may result in suspension or revocation of permission to
perform animal research in Wayne State University facilities.
7. For more information please read the safety standard operation procedures (SOPs). The PI and OEHS will maintain a copy of the SOPs.
Signature of Principal Investigator
(must be original; no copies, image files, etc.)
Date
Approval Date (to be filled in by OEH&S):