Membrane Biology Group IDG
ANNUAL REPORT
2002-2003
Dr Peter Flatman
Convener
Membrane Biology Group IDG
October 2003
Membrane Biology Group IDG — Annual Report 2002-2003
CONTENTS
1. INTRODUCTION
1
2. SUCCESSES AND ADVANCES
1
2.1. Key Publications
2
2.2. MBG Hosts International Meeting
2
2.3. Catalyst BioMedica Award
2
2.4. Wellcome Trust Biomedical Image Awards (2002-2003)
2
2.5. Student successes
2
3. MEMBERSHIP
3
4. NEW MEMBERS
4
5. GRANTS
5
6. STUDENTSHIPS AND FELLOWSHIPS
7
6.1. New Studentships
7
6.2. New Post-doctoral Fellows
7
7. PUBLICATIONS
7
Membrane Biology Group IDG Report 2002-2003
MBG
1. INTRODUCTION
The Membrane Biology Group (MBG) strives to understand membrane-based mechanisms for the
regulation of cell function and how perturbations to these may underlie disease.
The MBG is an interdisciplinary research group (IDG) within the College of Medicine and Veterinary
Medicine with a focus on biological processes occurring at cell membranes. Currently the group
comprises 8 Principal Investigators (PIs). We are strongly research-oriented with related interests, and
we collaborate closely in our scientific investigations and in our teaching, gaining from the sharing of
our knowledge and expertise. Our members are drawn from different disciplines, and the techniques
we use therefore span many fields. Our laboratories are currently located in the Hugh Robson
Building and New Medical Extension in the George Square area of the University.
2. SUCCESSES AND ADVANCES
This has been a highly successful period for the group. At the start of the 2002/3 academic year MBG
PIs were major participants, often in collaboration with others outside the College, on grants worth
£10.4 million. During the period we activated a further £1.5 million of grants, including a new
programme grant. Our multidisciplinary group makes use of techniques from molecular biology,
physiology, chemistry, physics and genetics and interacts across Centres and Colleges. There are two
major areas of continued development. The first is in laser-based imaging technology. We now have
world-class facilities for imaging live cells and monitoring intracellular protein-protein interactions in
real time using confocal and multiphoton microscopy (supported by SHEFC/JREI) and fluorescence
lifetime imaging (FLIM). We also have one of the few Total Internal Reflectance Microscopes
(TIRFM) in the world and this is capable of imaging events that occur very close to the cell
membrane. These facilities are being exploited to address fundamental issues in membrane biology
(exemplified by a recent Nature paper resulting from extensive collaboration within the MBG) as well
as providing the basis for commercially exploitable projects (exemplified by a recent Catalyst
BioMedica (Wellcome Trust Technology Transfer) Development Fund award). Our expertise
complements that of COSMIC (Collaborative Optical Spectroscopy Micromanipulation and Imaging
Centre based in the College of Science and Engineering, King’s Buildings), with whom we
collaborate, and is a basis for an application for MRC earmarked studentships. The second major area
is in functional analysis of protein diversity. Upgrading of our facilities for proteomics (including a
mass spectrometer and new protein separation/analysis facilities as part of EPIC (Edinburgh Proteins
Interaction Centre, a cross College collaborative project)) and real time analysis of mRNA expression
is facilitating our understanding of the functional impact of pre-mRNA splicing and postranslational
protein modification in diverse cellular pathways. In addition to joint investment in facilities our close
collaboration with other IDGs within the Division of Biomedical Sciences has been developed,
including joint research retreats and seminar programmes, and culminating in several cross-IDG
collaborative projects. These joint ventures are ultimately aimed at establishing a Centre for
Integrative Physiology (CIP). Plans are being drawn up to house the entire MBG in adjacent
laboratories within the Hugh Robson Building to facilitate further collaboration and provide improved
access to core facilities. Centralization and improvement of imaging equipment including laser
confocal microscopes within the Hugh Robson Building will be of particular benefit as will be the
upgrading of animal facilities.
In the sections below we highlight some of the successes of our staff and students. Three of this year’s
publications were in very high impact journals including Nature and Cell, and we were delighted that
our TIRFM and confocal images have been used on the front covers of Nature and Biochemical
Society Transactions. A full list of our publications can be found at the end of this report. During the
year we also hosted a highly successful international meeting, one of our post-doctoral fellows
received a Wellcome Trust Catalyst BioMedica Award and five of our post-graduates received PhD
degrees.
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Membrane Biology Group IDG Report 2002-2003
MBG
2.1. Key Publications
1. Duncan RR, Greaves J, Wiegand UK, Matskevich I, Bodammer G, Apps DK, Shipston MJ, Chow RH
(2003), Functional and spatial segregation of secretory vesicle pools according to vesicle age, Nature 422:
176-180
2. Tan TC, Valova VA, Malladi CS, Graham ME, Berven LE, Jupp OJ, Hansra G, McClure SJ, Sarcevic B,
Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003), Cyclin dependent kinase 5 (Cdk5) is essential for
synaptic vesicle endocytosis, Nature Cell Biology 5: 701-710
3. Chen H-K, Fernandez-Funez P, Acevedo SF, Lam YC, Kaytor MD, Fernandez MH, Aitken A, Skoulakis
EMC, Orr HT, Botas J, Zoghbi HY (2003), Interaction of Akt-Phosphorylated Ataxin-1 with 14-3-3
Mediates Neurodegeneration in Spinocerebellar Ataxia Type 1, Cell 113: 457-468
2.2. MBG Hosts International Meeting
During the year the MBG hosted an international meeting called Molecular Mechanisms of Exocytosis
and Endocytosis (23-25 March 2003, National e-science centre, South College Street, Edinburgh )
The principal organiser was Dr Mike Cousin with Prof Mike Shipston, Dr David Apps and associate
member Dr Robert Chow serving on the organizing committee. Major sponsors of the meeting were:
The Wellcome Trust (£5000), International Society for Neurochemistry (£4200), IUBMB (£2500)
This biannual meeting brings together senior scientists from leading international laboratories to
stimulate networking and exchange of ideas between the next generation of principal investigators in
the field of exocytosis and endocytosis. In a short space of time these meetings have become one of
the premier European conferences in the field and attract participants (83 in total) from all over the
globe.
2.3. Catalyst BioMedica Award
Dr Rory Duncan who has been working with Prof Mike Shipston was awarded a prestigious Catalyst
BioMedica Fellowship (Wellcome Trust Technology Transfer).
2.4. Wellcome Trust Biomedical Image Awards (2002-2003)
Dr Rory Duncan and Ms Linda Wilson won an “Award of Excellence” in this year’s Wellcome Trust
“Truth and Beauty” Biomedical Image Awards. The award was for an image of a PC12 cell modified
to express a fluorescently labelled hormone. The wining images are on display in the Trust’s “20-20”
Gallery, alongside original artworks inspired by the images. The image was also chosen to decorate a
huge billboard outside the Wellcome Trust’s headquarters building in Euston Road during building
work.
2.5. Student successes
During the year, six of our post-graduate students were awarded with either a PhD or MSc degree:
2

Eva Zemlickova (PhD)

Nick Karoulias (PhD)

Hasan Almulla (PhD)

Lorraine Coghill (PhD)

Sampol Tapechum (PhD)

Jenny Greaves (MSc with distinction)
Membrane Biology Group IDG Report 2002-2003
MBG
3. MEMBERSHIP
During this period the Membrane Biology Group comprised 8 Principal Investigators. This increased
to 9 in October 2003 when Dr Rory Duncan also became a PI. The group has over 40 additional
research staff as described in the table below. Dr Peter Flatman has been convenor since February
2002. The group has a SHEFC-funded Laboratory Manager – Mark Patrizio.
PRINCIPAL INVESTIGATOR
MEMBERSHIP OF RESEARCH GROUP
Prof Alastair Aitken
Post doctoral fellows
 Dr Helen Baxter (AR2)
 Dr Rob Wakefield (AR1A)
 Dr Alex Peden (AR1A, until end January 2003)
 Dr Shaun Mackie (AR1A, until end August 2003)
Research and technical assistants
 Gaynor Campbell (joint with Chemistry)
 Henrietta Shaw (TGD, until end September 2003)
Post graduate students
 Sam Clokie (AR1B, PhD)
 Carolyn Brechin (PhD, MRC studentship)
 Ross Stevenson (PhD, joint with Chemistry,)
 Eva Zemlickova (PhD)
Dr David Apps
Post doctoral fellows
 Dr Ulrich Wiegand (AR2)
Post graduate students
 Angela McDonald (PhD) College Scholarship
Dr Richard Ashley
Research and technical assistants
 Nick Karoulias (AR1B) until end December 2002
Post graduate students
 Heather Findlay (PhD) College Scholarship
Dr Mike Cousin
Post doctoral fellows
 Dr Ruth Fowler 2001- (AR1A)
 Dr Orla Jupp 2001-end August 2003 (AR1A)
Post graduate students
 Karen Smillie 2001- (PhD) College Scholarship
Dr Peter Flatman
Post doctoral fellows
 Dr Ioulia Matskevich (AR1A)
 Dr Mike Steele (AR1A, until July 2003)
Research and technical assistants
 Catherine Naughton (TGC/D, until February 2003)
 Catriona Armstrong (TGC, from July 2003)
Post graduate students
 Hasan Almullah (PhD, State of Bahrain, completed 2002)
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Membrane Biology Group IDG Report 2002-2003
MBG
PRINCIPAL INVESTIGATOR
MEMBERSHIP OF RESEARCH GROUP
Dr Andrew Hall
Post doctoral fellows
 Dr Peter Bush
Post graduate students
 Mark Kerrigan (PhD, completed 2002) MRC Studentship
Dr Rory Mitchell
Post doctoral fellows
 Melanie Johnson 1987- (MRC External Staff & UoE
Honorary Fellow)
Research and technical assistants
 Pamela Holland 1997- (TGD)
Post graduate students
 Derek Robertson 2001-2004 (PhD) MRC Studentship
 Daniel Collins 2000-2003 (PhD) MRC Studentship
Prof Mike Shipston
Post-doctoral fellows
 Dr Lie Chen 2003- (AR1A)
 Dr Rory Duncan 1998- (AR2)
 Dr Stuart Rivers 2002- (AR1A)
 Dr Iain Rowe 2003- (AR2)
 Dr Lijun Tian 1996- (AR2)
 Dr Helene Widmer 2001- (AR2)
Research and technical assistants
 Eliane Chirnside 2002- (TGD)
 Jenny Greaves 2001- (AR1B)
 Heather McClafferty 2003- (AR1B)
 Jean-Marc Huibant 2003- (TGD)
Post graduate students
 Stephen MacDonald 2001- (PhD) BBSRC Committee
studentship
 Lorraine Coghill 1999-2003 (PhD) College Scholarship
 Sompol Tapechum 1999-2003 (PhD) self-funded
4. NEW MEMBERS
 Dr Rory Duncan will become a full member (PI) of the MBG in October 2003
4
MBG
Membrane Biology Group IDG Report 2002-2003
5. GRANTS
MBG Research Grants active at 1 October 2002
Principal MBG
Applicant
Awarding
Body
Project Title
Award
Start
End
Aitken A
MRC
Protein complexes that regulate
intracellular signal transduction:
structure and regulation by
phosphorylation
£1,650,000
1/3/98
28/2/03
Aitken A (joint with P
Sadler and 8 others in
Chemistry and ICMB
JIR/
Wellcome
Edinburgh Protein Interactions
Centre (EPIC)
£4,741,409
Nov 00
Nov 05
Aitken A (joint with M
Dutia & J Seckl)
Wellcome
Mechanisms of vestibular neuronal
plasticity and its facilitation by
glucocorticoids
£111,650
1/10/01
30/9/03
Aitken A
MRC
Development of diagnostics for
TSE
£393,615
1/3/02
28/2/05
Aitken (joint with R
Baxter, A Jones, G
Whittaker (Chemistry),
H Baxter (BMS), J
Barton (Physics), J
Ironside (WGH)
Dept of
Health
Removal of infective protein
residues from medical instruments
£1,145,479
May 02
May 05
Apps D
Wellcome
In vivo and in vitro studies of the
maturation, mobility and fusion
competence of 'time stamped'
secretory vesicles in
neuroendocrine cells
£206,973
1/7/02
30/6/05
Ashley R
Wellcome
Expression and function of an
endoplasmic reticulum chloride
channel protein
£131,997
6/1/99 31/12/02
Cousin M
Cunningham
Trust
Do neurones release glutamate by
a different mechanism during
epilepsy?
Cousin M
Wellcome
Flatman P
£65,938
1/9/01
30/8/03
Control of synaptic vesicle
trafficking by PI-3-kinase in
neurones
£183,921
1/1/01
31/3/04
Wellcome
The regulation of sodium
potassium/chloride cotransport by
phosphorylation
£166,190
1/6/00 31/12/03
Flatman P
BHF
Regulation of magnesium
transport across the plasma
membrane of cardiac myocytes
£109,863
1/3/01
Hall A
Arthritis
Research
Campaign
Regulation of cell volume by
chondrocytes from normal and
degenerate cartilage
£116,639 17/11/00 16/11/03
Hall A
JREI/SHEFC
Visualisation of cell biology
dynamics studied by confocal and
multi-photon imaging
£250,000
2002
Dec 04
Mitchell R
MRC
Membrane and Adapter Proteins
Co-operative Group
£147,434
1/10/99
30/9/04
29/2/04
5
MBG
Membrane Biology Group IDG Report 2002-2003
Principal MBG
Applicant
Awarding
Body
Project Title
Award
Start
End
Mitchell R
MRC
Functional association of G
protein-coupled receptors with the
small G proteins ARF and Rho
£157,897
1/10/99
31/7/03
Shipston M
MRC
Regulation of ion channel activity
by glucocorticoids: a molecular
genetic and proteomic analysis
£289,492
1/1/02 31/12/04
Shipston M
Wellcome
BK channel subunit expression in
identified mammalian CNS
neurones
£209,437
1/10/99 30/11/03
Shipston M
Wellcome
Spacio-temporal studies of
interactions of DOC2 with Munc13
and Munc18 in vivo
£281,383
1/8/00
31/7/03
Total of existing grants: £10,359,317
New MBG Grants from 1 October 2002 – 30 September 2003
Principal
Applicant
Awarding
Body
Project Title
Award
Start
End
Cousin M
Wellcome
Control of synaptic vesicle
recycling by the phosphorylation
status of dynamin I (to be
activated)
£183,000
2003
2006
Duncan R
Wellcome
Use of photoactive proteins as
acceptor molecules in resonance
energy transfer interaction assays
£187,401
1/1/03
31/12/04
Hall A
Royal College of
Surgeons of Edin
Physiology of the Human Growth
Plate
£5,800
Aug 03
Jul 04
Shipston M
Wellcome
Alternative splicing of calciumactivated potassium channels
£835,580
1/10/02
30/9/07
Shipston M
BBSRC
Targeting of kinase/phosphatase
signaling complexes to BK
channels mediated by leucine
zipper domains
£275,528
15/2/03
15/2/06
Total of new grants: £1,487,309
6
Membrane Biology Group IDG Report 2002-2003
MBG
6. STUDENTSHIPS AND FELLOWSHIPS
6.1. New Studentships

Carolyn Brechin (MRC)
6.2. New Post-doctoral Fellows



Dr Lie Chen
Dr Iain Rowe
Dr Stuart Rivers
7. PUBLICATIONS
1.
Aitken A (2002) Functional specificity in 14-3-3 isoform interactions through dimer formation and
phosphorylation. Chromosome location of mammalian isoforms and variants, Plant Molecular Biology 50:
993-1010
2.
Aitken A (2003) Identification of phosphoamino acids by thin layer electrophoresis, in Proteins and
Proteomics. A Laboratory Manual, ed. Simpson R, Cold Spring Harbor Laboratory Press, p 880-881
3.
Aitken A (2003) Modification of phosphoamino acids to facilitate identification, in Proteins and
Proteomics. A Laboratory Manual, ed. Simpson R, Cold Spring Harbor Laboratory Press, p 877-879
4.
Aitken A (2003) Protein consensus sequence motifs, Methods in Molecular Biology 211: 465-485
5. Aitken A (2003) Solid Phase microsequencing of phosphopeptides, in Proteins and Proteomics. A
Laboratory Manual, ed. Simpson R, Cold Spring Harbor Laboratory Press, p 329-331
6.
Aitken A, Baxter H, Dubois T, Clokie S, Mackie S, Mitchell K, Peden A, Zemlickova E (2002) Specificity
of 14-3-3 isoform dimer interactions and phosphorylation, Biochemical Society Transactions 30: 351-360
7.
Aitken A, Learmonth M (2002) Protein identification by in-gel digestion and mass spectrometric analysis,
Molecular Biotechnology 20: 95-97
8.
Aitken A, Learmonth M (2003) Analysis of sites of protein phosphorylation, Methods in Molecular Biology
211: 385-398
9.
Aitken A, Learmonth M (2003) Quantitation and location of disulfide bonds in proteins, Methods in
Molecular Biology 211: 399-410
10.
Ashley RH (2002) Challenging accepted ion channel biology: p64 and the CLIC family of putative
intracellular anion channel proteins, Molecular Membrane Biology 20: 1-11
11.
Baxter H, Liu WG, Forster JL, Aitken A, Fraser JR (2002) Immunolocalisation of 14-3-3 isoforms in
normal and scrapie-infected murine brain, Neuroscience 109: 5-14
12.
Baxter HC, Fraser JR, Liu WG, Forster JL, Clokie S, Steinacker P, Otto M, Bahn E, Wiltfang J, Aitken A
(2002) Specific 14-3-3 isoform detection and immunolocalization in prion diseases, Biochemical Society
Transactions 30: 387-391
13.
Bush PG, Hall AC (2003) The volume and morphology of chondrocytes within normal and degenerate
human articular cartilage, Osteoarthritis and Cartilage 11: 242-251
14.
Bush PG, Huntley JS, Brenkel IJ, Hall AC (2003). The shape of things to come – chondrocytes and
osteoarthrosis. Clinical and Investigative Medicine 26(5):249-251
15.
Chaudhri M, Scarabel M, Aitken A (2003) Mammalian and yeast 14-3-3 isoforms form distinct patterns of
dimers in vivo, Biochemical and Biophysical Research Communications 300: 679-685
16.
Chen H-K, Fernandez-Funez P, Acevedo SF, Lam YC, Kaytor MD, Fernandez MH, Aitken A, Skoulakis
EMC, Orr HT, Botas J & Zoghbi HY (2003) Interaction of Akt-Phosphorylated Ataxin-1 with 14-3-3
Mediates Neurodegeneration in Spinocerebellar Ataxia Type 1. Cell 113: 457-468
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Membrane Biology Group IDG Report 2002-2003
MBG
17.
Chin MC, Shipston MJ & Antoni FA (2002) Post-translational modulation of glucocorticoid feedback
inhibition at the pituitary level. Endocrinology 143: 3796-3801
18.
Cousin MA, Malladi CS, Tan TC, Raymond CR, Smillie KJ, Robinson PJ (2003) Synapsin l-associated
phosphatidylinositol 3-kinase mediates synaptic vesicle delivery to the readily releasble pool, Journal of
Biological Chemistry 278: 29065-29071
19.
Cousin MA (2003) Use of FM1-43 and other derivatives to investigate neuronal function. Current
Protocols in Neuroscience. Unit 2.6: 1-12
20.
Dubois T, Howell S, Zemlickova E, Aitken A (2002) Identification of casein kinase Ialpha interacting
protein partners, FEBS Letters 517: 167-171
21.
Dubois T, Howell S, Zemlickova E, Learmonth M, Cronshaw A, Aitken A (2003) Novel in vitro and in
vivo phosphorylation sites on protein phosphatase 1 inhibitor CPI-17, Biochemical and Biophysical
Research Communications 302: 186-192
22. Dubois T, Kerai P, Learmonth M, Cronshaw A, Aitken A (2002) Identification of syntaxin-1A sites of
phosphorylation by casein kinase I and casein kinase II, European Journal of Biochemistry 269: 909-914
23.
Dubois T, Zemlickova E, Howell S, Aitken A (2003) Centaurin-alpha(1) associates in vitro and in vivo with
nucleolin, Biochemical and Biophysical Research Communications 301: 502-508
24.
Duncan RR, Greaves J, Tapechum S, Apps DK, Shipston MJ, Chow RH (2002) Efficacy of Semliki Forest
virus transduction of bovine adrenal chromaffin cells: an analysis of heterologous protein targeting and
distribution, Annals of the New York Academy of Sciences 971: 641-646
25.
Duncan RR, Greaves J, Wiegand UK, Matskevich I, Bodammer G, Apps DK, Shipston MJ, Chow RH
(2003) Functional and spatial segregation of secretory vesicle pools according to vesicle age, Nature 422:
176-180
26.
Erxleben C, Everhart AL, Romeo C, Florance H, Bauer MB, Alcorta DA, Rossie S, Shipston MJ &
Armstrong DL (2002) Interacting effects of N-terminal variation and STREX-exon splicing on slo
potassium channel regulation by calcium, phosphorylation and oxidation. Journal of Biological Chemistry
277: 27045-27052
27.
Erxleben C, Everhart A, Romeo C, Florance H, Bauer MB, Alcorta DA, Rossie S, Shipston MJ &
Armstrong DL (2002) Full length KCNMA1 channels cannot make heads and tails with STREX.
Biophysical Journal 82(1): 997
28.
Flatman PW (2002) Regulation of Na-K-2Cl cotransport by phosphorylation and protein- protein
interactions, Biochimica et Biophysica Acta 1566: 140-151
29.
Flatman PW (2003) Magnesium transport, in Red Cell Membrane Transport in Health and Disease, ed.
Bernhardt I and Ellory JC, Springer-Verlag, Berlin p 407-434
30.
Garry EM, Moss A, Rosie R, Delaney A, Mitchell R, Fleetwood-Walker SM. (2003) Specific involvement
in neuropathic pain of AMPA receptors and adapter proteins for the GluR2 subunit. Molecular and Cellular
Neuroscience 24: 10-22
31.
Garry EM, Moss A, Delaney A, O'Neill F, Blakemore J, Bowen J, Husi H, Mitchell R, Grant SG,
Fleetwood-Walker SM (2003) Neuropathic Sensitization of Behavioral Reflexes and Spinal NMDA
Receptor/CaM Kinase II Interactions Are Disrupted in PSD-95 Mutant Mice. Curr Biol 13(4):321-328
32.
Graeser R, Gannon J, Poon RY, Dubois T, Aitken A, Hunt T (2002) Regulation of the CDK-related protein
kinase PCTAIRE-1 and its possible role in neurite outgrowth in Neuro-2A cells, Journal of Cell Science
115: 3479-3490
33.
Greaves J, Duncan RR, Tapechum S, Apps DK, Shipston MJ, Chow RH (2002) Use of ANF-EGFP for the
visualization of secretory vesicles in bovine adrenal chromaffin cells, Annals of the New York Academy of
Sciences 971: 275-276
34.
Hall AC, Bush PG, Davidson M, & Kempson SA (2003) Equine Articular Cartilage Chondrocytes –
Opening the Black Box. Equine Vet J 35(5), 425-428
35.
Henriksson ML, Francis MS, Peden A, Aila M, Stefansson K, Palmer R, Aitken A, Hallberg B (2002) A
nonphosphorylated 14-3-3 binding motif on exoenzyme S that is functional in vivo, European Journal of
Biochemistry 269: 4921-4929
36.
Huntley JS, Bush PG, Hall AC, Macnicol MF (2003) Looking at the living human growth plate, Canadian
Medical Association Journal 168: 459-460
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Membrane Biology Group IDG Report 2002-2003
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37.
Johnson MS, Lutz EM, Firbank S, Holland PJ, Mitchell R (2003) Functional interactions between native
Gs-coupled 5-HT receptors in HEK-293 cells and the heterologously expressed serotonin transporter. Cell
Signal 15(8):803-811
38.
Martin NC, McCullough CT, Bush PG, Sharp L, Hall AC, Harrison DJ (2002) Functional analysis of mouse
hepatocytes differing in DNA content: volume, receptor expression, and effect of IFNgamma, Journal of
Cellular Physiology 191: 138-144
39.
McCulloch DA, MacKenzie CJ, Johnson MS, Robertson DN, Holland PJ, Ronaldson E, Lutz EM, Mitchell
R (2002) Additional signals from VPAC/PAC family receptors, Biochemical Society Transactions 30: 441446
40.
Mitchell R, Robertson DN, Holland PJ, Collins D, Lutz EM, Johnson MS (2003) ADP-ribosylation factordependent phospholipase D activation by the M3 muscarinic receptor. J Biol Chem 278(36):33818-33830
41.
Moss A, Blackburn-Munro G, Garry EM, Blakemore JA, Dickinson T, Rosie R, Mitchell R, FleetwoodWalker SM (2002) A role of the ubiquitin-proteasome system in neuropathic pain. J Neurosci 22(4):13631372
42.
Pollard HB, Apps DK (2002) New technologies in exocytosis and ion movement, Annals of the New York
Academy of Sciences 971: 617-619
43.
Proutski I, Karoulias N, Ashley RH (2002) Overexpressed chloride intracellular channel protein CLIC4
(p64H1) is an essential component of novel plasma membrane anion channels, Biochemical and
Biophysical Research Communications 297: 317-322
44.
Robertson DN, Johnson MS, Moggach LO, Holland PJ, Lutz EM, Mitchell R (2003) Selective interaction of
ARF1 with the carboxy-terminal tail domain of the 5-HT2A receptor. Molecular Pharmacology 64: 1-13
45.
Ronaldson E, Robertson DN, Johnson MS, Holland PJ, Mitchell R, Lutz EM (2002) Specific interaction
between the hop1 intracellular loop 3 domain of the human PAC(1) receptor and ARF, Regulatory Peptides
109: 193-198
46.
Sribar J, Sherman NE, Prijatelj P, Faure G, Gubensek F, Fox JW, Aitken A, Pungercar J, Krizaj I (2003)
The neurotoxic phospholipase A(2) associates, through a non-phosphorylated binding motif, with 14-3-3
protein gamma and varepsilon isoforms, Biochemical and Biophysical Research Communications 302: 691696
47.
Tan TC, Valova VA, Malladi CS, Graham ME, Berven LE, Jupp OJ, Hansra G, McClure SJ, Sarcevic B,
Boadle RA, Larsen MR, Cousin MA, Robinson PJ (2003) Cyclin dependent kinase 5 (Cdk5) is essential for
synaptic vesicle endocytosis, Nature Cell Biology 5: 701-710
48.
Tian L., Coghill LC, MacDonald SH-F, Armstrong DL & Shipston MJ (2003) Leucine zipper domain
targets PKA to mammalian BK channels. Journal of Biological Chemistry 278:8669-8677
49.
Widmer H, Rowe ICM & Shipston MJ (2003) Conditional protein phosphorylation regulates BK channel
activity in rat cerebellar Purkinje neurones. Journal of Physiology (in press)
50.
Wiegand UK, Don-Wauchope A, Matskevich I, Duncan RR, Greaves J, Shipston MJ, Apps DK, Chow RH
(2002) Exocytosis studies in a chromaffin cell-free system: imaging of single-vesicle exocytosis in a
chromaffin cell-free system using total internal reflection fluorescence microscopy, Annals of the New York
Academy of Sciences 971: 257-261
51.
Wiegand UK, Duncan RR, Greaves J, Chow RH, Shipston MJ, Apps DK (2003) Red, yellow, green go! – a
novel tool for microscopic segregation of secretory vesicle pools according to their age. Biochem Soc Trans
31: 851-856
52.
Zemlickova E, Dubois T, Kerai P, Clokie S, Cronshaw AD, Wakefield RID, Johannes F-J & Aitken A
(2003) Centaurin-a1 associates with and is phosphorylated by isoforms of protein kinase C. Biochem
Biophys Res Commun 306, 459-465
Dr Peter Flatman
Convener
MBG Group IDG
October 2003
9