Mapping, Expression, SNP Identification and Association Analysis with Growth traits
of the Porcine INPP5F Gene
Quan-Yong Zhou1
Zhen-Fang Wu2
Shu-Hong Zhao1
1
Key Laboratory of Agricultural Animal Genetics, Breeding, and Reproduction of Ministry of
Education & Key Laboratory of Swine Genetics and Breeding of Ministry of Agriculture,
Huazhong Agricultural University, Wuhan, P.R. China; 2Huanong Wenshi cooperation,
Guangdong, P.R. China
INPP5F (inositol polyphosphate-5-phosphatase F) is a member of the large family of
phosphoinositide phosphatases, 5-phosphatase genes. Genes in this family contain a
Sac domain whose amino acids are essential for inositol polyphosphate phosphatase
activities. Increasing expression of this gene will prevent cell hypertrophy through
activating the glycogen synthase kinase 3β(Gsk3β) via inactivating thymoma viral
proto-oncogene (Akt) and 3-phosphoinositide-dependent protein kinase-1 (Pdk1).
Here, we cloned partial cDNA sequence of the INPP5F gene, assigned this gene to
C14G by using somatic cell hybrid panel (SCHP). Sequencing of PCR products of
INPP5F from different breeds identified a synonymous A/G polymorphism in the last
exon. The allele frequency analysis of this SNP showed that the Yorkshire and Duroc
pigs have high allele frequencies at the G allele （32 Yorkshire pigs, allele frequency
of G is 0.641; 30 Duroc pigs, allele frequency of G is 0.717）, whereas the local pig
breeds have high allele frequencies at the A allele (31 Dahuabai pigs, allele frequency
of A is 0.742; 26 Erhuanian pigs, allele frequency of A is 1; 30 Yusan pigs, allele
frequency of A is 0.850). Association analysis with growth and carcass traits in 232
Yorkshire pigs showed that different genotypes of INPP5F have significant
differences in average daily gain from birth to market and average daily gain from 30Kg
to 100Kg (ADG) (p<0.05). Expression analysis showed that this gene was expressed in
all the tissues examined. The imprinting analysis found that INPP5F were not
imprinted in porcine placenta tissues, although one transcript of this gene showed
monoallelic expression in the mouse brain.